##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935947.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681865
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19261290724704	33.0	31.0	34.0	30.0	34.0
2	32.300128324521715	34.0	31.0	34.0	30.0	34.0
3	32.35650751981697	34.0	31.0	34.0	30.0	34.0
4	35.886134352107824	37.0	35.0	37.0	35.0	37.0
5	35.92149032433106	37.0	35.0	37.0	35.0	37.0
6	35.943792392922354	37.0	35.0	37.0	35.0	37.0
7	36.08245767123991	37.0	35.0	37.0	35.0	37.0
8	35.80401105790736	37.0	35.0	37.0	35.0	37.0
9	37.540260902084725	39.0	37.0	39.0	35.0	39.0
10	37.239626612305955	39.0	37.0	39.0	34.0	39.0
11	37.19925058479318	39.0	37.0	39.0	34.0	39.0
12	37.16987966826278	39.0	37.0	39.0	34.0	39.0
13	37.19236945729727	39.0	37.0	39.0	34.0	39.0
14	38.44277092972949	40.0	38.0	41.0	34.0	41.0
15	38.57883891972751	40.0	38.0	41.0	34.0	41.0
16	38.66078182631459	40.0	38.0	41.0	35.0	41.0
17	38.63053390333864	40.0	38.0	41.0	35.0	41.0
18	38.59480982305882	40.0	38.0	41.0	35.0	41.0
19	38.58635360371921	40.0	38.0	41.0	34.0	41.0
20	38.455304202444765	40.0	38.0	41.0	34.0	41.0
21	38.42689535318575	40.0	38.0	41.0	34.0	41.0
22	38.415953304539755	40.0	38.0	41.0	34.0	41.0
23	38.413278288224205	40.0	38.0	41.0	34.0	41.0
24	38.321166213253356	40.0	37.0	41.0	34.0	41.0
25	38.23055003556423	40.0	37.0	41.0	34.0	41.0
26	38.079813452809574	40.0	37.0	41.0	34.0	41.0
27	38.0510863587367	40.0	37.0	41.0	34.0	41.0
28	38.05007002852471	40.0	37.0	41.0	34.0	41.0
29	38.03957674906323	40.0	37.0	41.0	34.0	41.0
30	37.93960388053354	40.0	37.0	41.0	34.0	41.0
31	37.96407646674928	40.0	37.0	41.0	34.0	41.0
32	37.91048961304657	40.0	37.0	41.0	34.0	41.0
33	37.871236975061045	40.0	37.0	41.0	33.0	41.0
34	37.817482932838615	40.0	37.0	41.0	33.0	41.0
35	37.792818226481785	40.0	37.0	41.0	33.0	41.0
36	37.72319887367734	40.0	36.0	41.0	33.0	41.0
37	37.686896966408305	40.0	36.0	41.0	33.0	41.0
38	37.61940413424945	40.0	36.0	41.0	33.0	41.0
39	37.56131052334406	40.0	36.0	41.0	33.0	41.0
40	37.44733634957066	40.0	36.0	41.0	33.0	41.0
41	37.347923709238636	40.0	35.0	41.0	33.0	41.0
42	37.33593746562736	40.0	35.0	41.0	33.0	41.0
43	37.29806633277848	40.0	35.0	41.0	33.0	41.0
44	37.24632881875444	39.0	35.0	41.0	33.0	41.0
45	37.1326611572672	39.0	35.0	41.0	32.0	41.0
46	37.0678741393091	39.0	35.0	41.0	32.0	41.0
47	37.02720919830172	39.0	35.0	41.0	32.0	41.0
48	36.95078351286545	39.0	35.0	41.0	32.0	41.0
49	36.89813086168083	39.0	35.0	41.0	32.0	41.0
50	36.780972773202905	39.0	35.0	41.0	32.0	41.0
51	35.863393780293755	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	8.0
10	21.0
11	20.0
12	16.0
13	22.0
14	27.0
15	29.0
16	49.0
17	68.0
18	149.0
19	264.0
20	396.0
21	637.0
22	985.0
23	1390.0
24	2079.0
25	2862.0
26	3888.0
27	4793.0
28	5712.0
29	6516.0
30	8380.0
31	10557.0
32	13782.0
33	19530.0
34	36898.0
35	47583.0
36	47268.0
37	71058.0
38	133404.0
39	263216.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91406656742904	21.222529386315472	24.060481180292285	11.802922865963204
2	31.319689381329148	24.753433597559635	25.58189670975926	18.344980311351954
3	32.80869380302553	24.600764080866448	25.595535773210237	16.995006342897785
4	29.603220578853584	26.09798127195266	24.856826497913808	19.441971651279946
5	25.81522735438833	30.091147074567548	23.785060092540313	20.30856547850381
6	26.042838391763766	35.366091528381716	23.416218752978963	15.174851326875554
7	80.56374795597368	6.5213788653179146	7.99205121248341	4.922821966224985
8	81.23382194422649	5.920820103686213	7.625263065269518	5.220094886817773
9	75.00868940332764	8.268058926620373	9.995380317218219	6.727871352833772
10	39.5287923562582	28.64525969216781	16.82591128742493	15.00003666414906
11	29.463163529437647	26.741070446496007	24.520982892508048	19.274783131558298
12	28.652592521980157	23.465788682510468	27.722203075388823	20.159415720120553
13	25.543032711753792	23.7981125296063	29.148731787083953	21.510122971555955
14	20.37030790552382	27.97357248135628	28.128001877204433	23.528117735915465
15	19.403400966466968	27.114018170752274	32.16267149655724	21.31990936622352
16	21.840833596093066	26.39158777763927	30.66765415441473	21.099924471852933
17	21.476098641226635	26.926004414363547	27.976065643492483	23.621831300917336
18	22.26804426096075	27.786731977737528	27.865779883114694	22.07944387818703
19	24.357607444288824	28.411489077749998	26.000454635448367	21.230448842512814
20	26.187295139067118	26.697806750603124	26.85150286347004	20.263395246859716
21	25.075784796110668	27.04846267222984	26.968534827275192	20.907217704384298
22	23.23993752429	25.36528491710236	27.185879902913335	24.208897655694308
23	22.900134190785565	26.808972450558393	27.839528352386466	22.45136500626957
24	22.67472300235384	25.60169535025262	28.64701957132277	23.076562076070775
25	22.815513334751014	27.447955240406824	26.287168281111363	23.449363143730796
26	21.56863895345853	29.08830926943017	26.49322079883848	22.849830978272827
27	22.89192142139573	28.196490507651806	26.671408563278654	22.24017950767381
28	20.425450785712716	28.670777939914792	27.187053155683312	23.716718118689183
29	21.897443042244433	27.076474082113027	26.838010456615315	24.188072419027225
30	22.26129805753338	26.681821181612197	26.68783410205833	24.369046658796094
31	22.46148431141062	27.752267677619468	24.66455970023392	25.121688310735994
32	23.19315407008719	28.81772784935435	24.4560140203706	23.533104060187867
33	22.81522002155852	28.112456278002245	24.16592727299392	24.906396427445316
34	21.5177491145608	27.49708520014959	26.611572672009853	24.373593013279756
35	21.826167936468362	26.791520315604995	25.547579066237454	25.834732681689193
36	21.536374502284175	30.42347092166338	24.765312781855645	23.2748417941968
37	21.470965660357987	28.215849178356418	26.793426851356205	23.519758309929383
38	21.82323480454342	27.58449253151284	25.486716578794923	25.105556085148823
39	23.212952710580538	26.090648442140306	26.68519428332588	24.011204563953275
40	23.275575077178033	25.7917622989888	29.662616500333645	21.27004612349952
41	19.76476281961972	28.370425230800816	27.948640859994278	23.91617108958518
42	21.08364558966951	28.39828998408776	26.652783175555278	23.865281250687453
43	22.123440857061148	27.261408049980567	26.583561262126665	24.031589830831614
44	22.48113629530772	26.202400768480565	25.990188673711074	25.326274262500643
45	22.17330409978515	25.1463266189055	26.59192068811275	26.0884485931966
46	21.966518299076796	27.563080668460767	26.002801140988318	24.46759989147412
47	21.1512542805394	26.6989800033731	28.151760245796453	23.99800547029104
48	20.725070211845452	25.7746034772279	28.139441091711703	25.360885219214946
49	21.510562941344695	25.55813834116724	28.40562281390011	24.525675903587953
50	20.014079033239717	24.49047832048866	29.47240289500121	26.02303975127041
51	20.266768348573397	24.692131140328364	26.92204468626488	28.11905582483336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	636.0
2	1052.0
3	1896.0
4	2740.0
5	1928.0
6	1116.0
7	1054.0
8	992.0
9	988.5
10	985.0
11	976.0
12	967.0
13	1095.0
14	1223.0
15	1161.0
16	1099.0
17	1152.5
18	1206.0
19	1331.0
20	1456.0
21	1912.5
22	2369.0
23	2434.0
24	2499.0
25	3139.0
26	4743.0
27	5707.0
28	6935.5
29	8164.0
30	10146.0
31	12128.0
32	13423.0
33	14718.0
34	16631.5
35	18545.0
36	19676.5
37	20808.0
38	22753.0
39	24698.0
40	27445.0
41	30192.0
42	32740.0
43	35288.0
44	40036.0
45	44784.0
46	50407.5
47	56031.0
48	60842.5
49	65654.0
50	64862.5
51	64071.0
52	57771.5
53	51472.0
54	46048.0
55	40624.0
56	38049.5
57	35475.0
58	34068.5
59	32662.0
60	31285.5
61	29909.0
62	27284.0
63	24659.0
64	20517.0
65	16375.0
66	13357.5
67	10340.0
68	8400.5
69	6461.0
70	5632.5
71	4804.0
72	3869.5
73	2935.0
74	2413.5
75	1424.5
76	957.0
77	666.5
78	376.0
79	295.0
80	214.0
81	180.0
82	146.0
83	95.0
84	44.0
85	28.5
86	13.0
87	11.0
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	681865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.08123020286819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9878918818572	21.354031173144644
2	10.359659072409201	6.232625787607462
3	3.728114613861048	3.3643882176669373
4	1.9313258001426419	2.3238662396331766
5	1.2333505084241418	1.8550350282365569
6	0.9392438286110791	1.6952165895043891
7	0.7940929364594742	1.672110469687632
8	0.6744986729919258	1.6231799883039386
9	0.636356996821365	1.722816118133041
>10	8.555326013510955	52.19037075038826
>50	0.1044603417886189	2.064725355965815
>100	0.053215645816085774	3.0942718256033506
>500	9.85474922520107E-4	0.21091918029476756
>1k	0.0014782123837801604	0.5964432758300384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1463	0.2145586003094454	No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1406	0.20619917432336313	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1155	0.16938836866535165	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	907	0.13301753279608133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.799395774823462E-4	0.0	0.0	0.13888379664596365	0.0
2	8.799395774823462E-4	0.0	0.0	0.6523285401069127	0.0
3	8.799395774823462E-4	0.0	0.0	0.950628056873428	0.0
4	8.799395774823462E-4	0.0	0.0	1.3732923672574484	0.0
5	8.799395774823462E-4	0.0	0.0	2.5434653487127217	0.0
6	8.799395774823462E-4	0.0	0.0	3.355062952343939	0.0
7	8.799395774823462E-4	0.0	0.0	4.09817192552778	0.0
8	8.799395774823462E-4	0.0	0.0	5.313955108415889	0.0
9	0.001026596173729404	0.0	0.0	5.793082208355027	0.0
10	0.001026596173729404	0.0	0.0	6.813372148445807	0.0
11	0.001026596173729404	0.0	0.0	8.089431192391455	0.0
12	0.001026596173729404	0.0	0.0	9.205634546427811	0.0
13	0.001026596173729404	0.0	0.0	9.64941740667141	0.0
14	0.001026596173729404	0.0	0.0	9.851510196299854	0.0
15	0.001026596173729404	0.0	0.0	10.164622029287322	0.0
16	0.001026596173729404	0.0	0.0	10.800378374018317	0.0
17	0.001026596173729404	0.0	0.0	11.535567891004817	0.0
18	0.001026596173729404	0.0	0.0	12.310941315363012	0.0
19	0.001026596173729404	0.0	0.0	12.792414920842102	0.0
20	0.001026596173729404	0.0	0.0	13.274181839513687	0.0
21	0.001026596173729404	0.0	0.0	13.932083330277987	0.0
22	0.001026596173729404	0.0	0.0	14.590718104023523	0.0
23	0.001026596173729404	0.0	0.0	15.223687973425825	0.0
24	0.001026596173729404	0.0	0.0	15.741532414774186	0.0
25	0.001026596173729404	0.0	0.0	16.21625981682591	0.0
26	0.001026596173729404	0.0	0.0	16.625725033547695	0.0
27	0.001026596173729404	0.0	0.0	17.048536000527964	0.0
28	0.0011732527699764616	0.0	0.0	17.489532385442867	0.0
29	0.0013199093662235194	0.0	0.0	17.963233191320864	0.0
30	0.0013199093662235194	0.0	0.0	18.509822325533648	0.0
31	0.0013199093662235194	0.0	0.0	19.008748065966138	0.0
32	0.0013199093662235194	0.0	0.0	19.490221671445227	0.0
33	0.0016132225587176348	0.0	0.0	19.925938418895235	0.0
34	0.0016132225587176348	0.0	0.0	20.351975830992938	0.0
35	0.0017598791549646923	0.0	0.0	20.79707860060276	0.0
36	0.0017598791549646923	0.0	0.0	21.28148533800679	0.0
37	0.0017598791549646923	0.0	0.0	21.751813042171104	0.0
38	0.0017598791549646923	0.0	0.0	22.226100474434087	0.0
39	0.0017598791549646923	0.0	0.0	22.694814956039686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGACA	40	6.8084773E-9	45.0	26
CGCATGT	20	7.031266E-4	45.0	38
ATCCGTG	20	7.031266E-4	45.0	10
TACGCGC	20	7.031266E-4	45.0	34
TCGTAAG	55	1.8189894E-12	45.0	1
CGCACGA	20	7.031266E-4	45.0	40
CGCATCG	25	3.8891318E-5	44.999996	21
GGTTAAC	25	3.8891318E-5	44.999996	9
AATACGG	55	6.002665E-11	40.909092	2
TTTGCGG	135	0.0	40.0	2
TACGGGA	320	0.0	38.671875	4
TAGAACG	35	6.2445524E-6	38.571426	1
TCTACGG	35	6.2445524E-6	38.571426	2
GGCGTAG	65	9.094947E-12	38.076927	1
TAGCGCG	30	1.1394351E-4	37.499996	1
TAACGCC	30	1.1394351E-4	37.499996	12
TCTAACG	30	1.1394351E-4	37.499996	32
CGTACTA	30	1.1394351E-4	37.499996	37
GCGATAC	30	1.1394351E-4	37.499996	9
TGTAGCG	30	1.1394351E-4	37.499996	1
>>END_MODULE