FastQCFastQC Report
Sat 14 Jan 2017
SRR2935946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380677
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC29090.7641648956989784TruSeq Adapter, Index 27 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC23520.6178466258796828No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG21360.5611056092172629No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC20240.5316843413182304TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC18560.48755243946968163TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT14100.3703927476574629TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC10140.26636755044302646TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC6670.17521415793441683TruSeq Adapter, Index 27 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.13003149651804546No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT4870.1279299773824003No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT4830.12687921781457773No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG4580.12031197051568653TruSeq Adapter, Index 27 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG4510.118473141271997TruSeq Adapter, Index 27 (100% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG4490.1179477614880857TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC4010.10533864667421461No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG3890.10218636797074684TruSeq Adapter, Index 27 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG650.045.0000041
CGAAAGG650.045.0000042
GAATCGG302.1616615E-645.0000042
TTACGAG351.209064E-745.0000041
ACCGGCA207.0273364E-445.037
GTCGACG207.0273364E-445.01
CACGGGT207.0273364E-445.04
TTTCGGC207.0273364E-445.041
GCGCACT207.0273364E-445.028
ACCTCCG253.8858732E-545.015
CGTTAAT207.0273364E-445.017
CCCCGTA207.0273364E-445.040
GAGCGAA207.0273364E-445.010
AACGCTA207.0273364E-445.043
GTTCCGC253.8858732E-545.013
GATGCGC207.0273364E-445.012
ACGCTAC207.0273364E-445.044
TTCGGTA207.0273364E-445.025
TACTAGG253.8858732E-545.02
CCGAACA207.0273364E-445.039
Produced by FastQC (version 0.11.5)