##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380677
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92139530363011	33.0	31.0	34.0	30.0	34.0
2	32.04956432881419	33.0	31.0	34.0	30.0	34.0
3	32.12377159639274	33.0	31.0	34.0	30.0	34.0
4	35.714308981104715	37.0	35.0	37.0	33.0	37.0
5	35.75616598848893	37.0	35.0	37.0	35.0	37.0
6	35.77440980148525	37.0	35.0	37.0	35.0	37.0
7	35.973770414288225	37.0	35.0	37.0	35.0	37.0
8	35.64460421827428	37.0	35.0	37.0	33.0	37.0
9	37.44906574339926	39.0	37.0	39.0	34.0	39.0
10	37.040837770603424	39.0	37.0	39.0	33.0	39.0
11	36.993734846076855	39.0	37.0	39.0	33.0	39.0
12	36.99672951084515	39.0	37.0	39.0	33.0	39.0
13	36.97060500109016	39.0	37.0	39.0	33.0	39.0
14	38.11572277810322	40.0	37.0	41.0	33.0	41.0
15	38.2439049377819	40.0	37.0	41.0	34.0	41.0
16	38.36217318093817	40.0	37.0	41.0	34.0	41.0
17	38.340222813566356	40.0	37.0	41.0	34.0	41.0
18	38.359829987101925	40.0	37.0	41.0	34.0	41.0
19	38.374472321679534	40.0	37.0	41.0	34.0	41.0
20	38.20006724861234	40.0	37.0	41.0	34.0	41.0
21	38.237813159187446	40.0	37.0	41.0	34.0	41.0
22	38.219306656299175	40.0	37.0	41.0	34.0	41.0
23	38.238372688657314	40.0	37.0	41.0	34.0	41.0
24	38.15623481324062	40.0	37.0	41.0	34.0	41.0
25	38.10870370419017	40.0	37.0	41.0	34.0	41.0
26	37.96067269627532	40.0	37.0	41.0	34.0	41.0
27	37.881739637540484	40.0	36.0	41.0	33.0	41.0
28	37.91093499213244	40.0	37.0	41.0	33.0	41.0
29	37.91641733017755	40.0	37.0	41.0	34.0	41.0
30	37.82215368934819	40.0	36.0	41.0	33.0	41.0
31	37.91509074622318	40.0	37.0	41.0	34.0	41.0
32	37.800150783997985	40.0	36.0	41.0	33.0	41.0
33	37.80097037646088	40.0	37.0	41.0	33.0	41.0
34	37.80828628995185	40.0	37.0	41.0	33.0	41.0
35	37.798769035166295	40.0	37.0	41.0	33.0	41.0
36	37.727277981070564	40.0	37.0	41.0	33.0	41.0
37	37.67796846145157	40.0	36.0	41.0	33.0	41.0
38	37.61800161291594	40.0	36.0	41.0	33.0	41.0
39	37.595278937261774	40.0	36.0	41.0	33.0	41.0
40	37.51973195123425	40.0	36.0	41.0	33.0	41.0
41	37.46932176096796	40.0	36.0	41.0	33.0	41.0
42	37.4741500011821	40.0	36.0	41.0	33.0	41.0
43	37.4011169574206	40.0	36.0	41.0	33.0	41.0
44	37.32051844477076	40.0	35.0	41.0	33.0	41.0
45	37.24070012110004	39.0	35.0	41.0	32.0	41.0
46	37.18538288365202	39.0	35.0	41.0	32.0	41.0
47	37.13587634661406	39.0	35.0	41.0	32.0	41.0
48	37.13382999235572	39.0	35.0	41.0	32.0	41.0
49	37.13947519813385	39.0	35.0	41.0	32.0	41.0
50	36.98536029232131	39.0	35.0	41.0	32.0	41.0
51	36.07697601903976	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	7.0
11	3.0
12	1.0
13	10.0
14	5.0
15	9.0
16	24.0
17	35.0
18	59.0
19	108.0
20	204.0
21	381.0
22	606.0
23	896.0
24	1245.0
25	1607.0
26	2217.0
27	2512.0
28	2996.0
29	3681.0
30	4850.0
31	6458.0
32	8624.0
33	11945.0
34	21110.0
35	29605.0
36	26049.0
37	39477.0
38	75010.0
39	140830.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07326158396751	21.287338084517845	25.394494545244395	10.24490578627025
2	30.82271847261589	25.440202586444677	25.18329187211205	18.55378706882738
3	30.500660665078268	26.460490126800412	26.333873598877787	16.704975609243533
4	27.950992573756757	25.01096730298915	28.102564641415164	18.935475481838935
5	26.10428263330855	29.281779566404065	25.159649781836045	19.454288018451336
6	24.278587884216805	34.793538879417454	25.32882207225548	15.599051164110255
7	76.64450439611534	5.85903535017876	11.57332856988996	5.923131683815938
8	76.149071259887	6.421454408855802	10.078097704878413	7.351376626378793
9	70.89895107926142	6.35578193586689	12.305707988662304	10.439558996209385
10	39.54980206316641	23.87089317190164	21.12105538290992	15.45824938202203
11	27.012138899907274	25.911730942505063	26.567142222934404	20.508987934653263
12	24.477706822319185	21.86052742876507	32.62687265056728	21.03489309834847
13	22.57924697315572	24.87699545809177	33.86414204167838	18.679615527074134
14	18.912621461238793	29.443071160064832	30.28709378291833	21.35721359577805
15	17.808798535241163	26.663812103174084	35.68300685357928	19.844382508005474
16	18.83066221494863	27.485506085211348	30.717379825941677	22.966451873898343
17	19.12329875458722	27.41720671330288	28.593006669696358	24.866487862413543
18	20.08553182882076	26.246660554748512	31.336802591173097	22.33100502525763
19	19.905589252831142	27.850382345137742	28.827326053320796	23.416702348710324
20	21.22613133969218	27.625782487515664	30.760198278330446	20.38788789446171
21	21.59599870756573	28.789761398771134	29.628267533893567	19.98597235976957
22	19.409893426710834	24.650294081334046	29.274424249429305	26.665388242525818
23	19.066295048032845	26.469158893234944	29.918539864504556	24.54600619422765
24	21.078499620413105	24.705984338428642	30.27606080745619	23.939455233702063
25	17.800392458698582	28.621114488135614	28.09678546379214	25.48170758937367
26	17.932525474352275	27.629722835895	29.005955179850634	25.431796509902092
27	20.93743514843292	27.939959598294617	27.368609083291084	23.753996169981377
28	15.917694003052457	26.15524447234795	30.230090076363954	27.69697144823564
29	18.112205360449934	24.03980277242912	28.958408309406664	28.889583557714282
30	19.16585451708404	27.55039048852439	29.403404986379527	23.88035000801204
31	19.195801164766984	26.008663512636698	26.56320187455507	28.232333448041253
32	19.109376190313572	26.960651681083963	26.644635741061318	27.285336387541143
33	18.266141637135945	25.286003619866708	28.764805859035352	27.68304888396199
34	16.715745894813715	22.735284768977372	31.218066760009144	29.33090257619977
35	16.473808504322562	23.71984648402714	30.63647133921934	29.169873672430956
36	18.045219438001244	24.83943080354211	29.184321616488518	27.931028141968124
37	17.223788145855938	24.73750712546332	32.07942691573171	25.959277812949033
38	16.984214964392386	24.534447838981606	29.39158394124152	29.089753255384487
39	19.436425105798357	21.761230649605835	31.826719239670375	26.975625004925437
40	19.013757069641716	21.681110232559362	34.73653517286308	24.568597524935836
41	16.487205688812303	24.412034349330273	30.43840316068478	28.66235680117265
42	18.586623305321833	22.42531069646971	31.205194955303316	27.78287104290514
43	20.505310276165883	22.971705671737457	28.188201546192705	28.334782505903956
44	19.042127577972927	22.96119807605923	28.791600228014826	29.205074117953018
45	19.507614066518332	21.481203224781115	29.073203792191283	29.937978916509273
46	21.980839399280754	22.69588128518403	30.543479117467037	24.779800198068177
47	16.702874090107887	21.848706383627064	34.81928248882911	26.629137037435935
48	17.54216829490618	21.219301402501333	32.3578782012047	28.88065210138779
49	19.38703940611069	19.35761813821166	33.96554033997326	27.289802115704394
50	18.747126829306733	19.50314833835509	32.62161885272817	29.12810597961001
51	17.702934508783034	19.332137218691962	29.632470572164856	33.332457700360145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	198.0
1	269.0
2	340.0
3	626.0
4	912.0
5	654.5
6	397.0
7	404.0
8	411.0
9	447.5
10	484.0
11	493.5
12	503.0
13	462.5
14	422.0
15	426.5
16	431.0
17	485.0
18	539.0
19	513.0
20	487.0
21	691.5
22	896.0
23	1186.5
24	1477.0
25	1677.5
26	2306.0
27	2734.0
28	3041.5
29	3349.0
30	4109.0
31	4869.0
32	5713.5
33	6558.0
34	7784.0
35	9010.0
36	10096.5
37	11183.0
38	12254.5
39	13326.0
40	14646.0
41	15966.0
42	19353.5
43	22741.0
44	25879.5
45	29018.0
46	38476.0
47	47934.0
48	50318.5
49	52703.0
50	50745.5
51	48788.0
52	39749.0
53	30710.0
54	26247.0
55	21784.0
56	18219.0
57	14654.0
58	12922.5
59	11191.0
60	9905.5
61	8620.0
62	7315.5
63	6011.0
64	5247.0
65	4483.0
66	3576.5
67	2670.0
68	2047.5
69	1425.0
70	1081.0
71	737.0
72	599.5
73	462.0
74	326.0
75	163.0
76	136.0
77	84.5
78	33.0
79	18.0
80	3.0
81	2.0
82	1.0
83	3.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	380677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.18152753998983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79695175742953	22.827879494064995
2	13.238296079438538	8.785337718848586
3	5.1237927468576965	5.100458104171764
4	2.6671888642103427	3.540056030085988
5	1.5263285040222943	2.5322955645643614
6	1.0298980093762569	2.050415349689938
7	0.724916031344122	1.6837674880757578
8	0.5900862647930297	1.5663970916959715
9	0.45132469488298416	1.3478078513463518
>10	5.680576528945772	40.56719321272295
>50	0.09967320017490017	2.141548747380766
>100	0.06458823371333532	4.055290048320361
>500	7.973856013992013E-4	0.1764780101931024
>1k	0.00558169920979441	3.625075288839125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2909	0.7641648956989784	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	2352	0.6178466258796828	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	2136	0.5611056092172629	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2024	0.5316843413182304	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1856	0.48755243946968163	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	1410	0.3703927476574629	TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1014	0.26636755044302646	TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	667	0.17521415793441683	TruSeq Adapter, Index 27 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	495	0.13003149651804546	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	487	0.1279299773824003	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	483	0.12687921781457773	No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	458	0.12031197051568653	TruSeq Adapter, Index 27 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	451	0.118473141271997	TruSeq Adapter, Index 27 (100% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	449	0.1179477614880857	TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	401	0.10533864667421461	No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	389	0.10218636797074684	TruSeq Adapter, Index 27 (100% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.880696758669423E-4	0.0	0.0	0.3921960086897816	0.0
2	7.880696758669423E-4	0.0	0.0	2.171920026689293	0.0
3	7.880696758669423E-4	0.0	0.0	3.0151545798669215	0.0
4	7.880696758669423E-4	0.0	0.0	4.137103108409492	0.0
5	7.880696758669423E-4	0.0	0.0	7.271781589116232	0.0
6	7.880696758669423E-4	0.0	0.0	8.622270323660215	0.0
7	7.880696758669423E-4	0.0	0.0	10.143770177867326	0.0
8	7.880696758669423E-4	0.0	0.0	12.393446412575491	0.0
9	7.880696758669423E-4	0.0	0.0	13.125300451563925	0.0
10	7.880696758669423E-4	0.0	0.0	15.340827000317855	0.0
11	7.880696758669423E-4	0.0	0.0	17.828762967029792	0.0
12	7.880696758669423E-4	0.0	0.0	20.243933833670013	0.0
13	7.880696758669423E-4	0.0	0.0	20.996540374122944	0.0
14	7.880696758669423E-4	0.0	0.0	21.294430711600647	0.0
15	7.880696758669423E-4	0.0	0.0	21.921208793806823	0.0
16	7.880696758669423E-4	0.0	0.0	23.189475592168687	0.0
17	7.880696758669423E-4	0.0	0.0	24.6807661088009	0.0
18	7.880696758669423E-4	0.0	0.0	26.263472707833674	0.0
19	7.880696758669423E-4	0.0	0.0	27.351534240313967	0.0
20	7.880696758669423E-4	0.0	0.0	28.282244527512827	0.0
21	7.880696758669423E-4	0.0	0.0	29.361899983450538	0.0
22	7.880696758669423E-4	0.0	0.0	30.4625706307447	0.0
23	7.880696758669423E-4	0.0	0.0	31.558250170091704	0.0
24	7.880696758669423E-4	0.0	0.0	32.42249991462579	0.0
25	7.880696758669423E-4	0.0	0.0	33.1929693677317	0.0
26	7.880696758669423E-4	0.0	0.0	33.88384378357505	0.0
27	7.880696758669423E-4	0.0	0.0	34.556592596873465	0.0
28	0.0010507595678225898	0.0	0.0	35.257974608395045	0.0
29	0.0010507595678225898	0.0	0.0	35.964610417755736	0.0
30	0.0010507595678225898	0.0	0.0	36.77133107595153	0.0
31	0.0015761393517338847	0.0	0.0	37.5071254633193	0.0
32	0.0015761393517338847	0.0	0.0	38.18854304305225	0.0
33	0.0015761393517338847	0.0	0.0	38.842903563913765	0.0
34	0.0015761393517338847	0.0	0.0	39.50278057250635	0.0
35	0.0015761393517338847	0.0	0.0	40.215458249382024	0.0
36	0.001838829243689532	0.0	0.0	40.882165195165456	0.0
37	0.001838829243689532	0.0	0.0	41.528119639484395	0.0
38	0.001838829243689532	0.0	0.0	42.16908297585617	0.0
39	0.001838829243689532	0.0	0.0	42.83500185196374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAG	65	0.0	45.000004	1
CGAAAGG	65	0.0	45.000004	2
GAATCGG	30	2.1616615E-6	45.000004	2
TTACGAG	35	1.209064E-7	45.000004	1
ACCGGCA	20	7.0273364E-4	45.0	37
GTCGACG	20	7.0273364E-4	45.0	1
CACGGGT	20	7.0273364E-4	45.0	4
TTTCGGC	20	7.0273364E-4	45.0	41
GCGCACT	20	7.0273364E-4	45.0	28
ACCTCCG	25	3.8858732E-5	45.0	15
CGTTAAT	20	7.0273364E-4	45.0	17
CCCCGTA	20	7.0273364E-4	45.0	40
GAGCGAA	20	7.0273364E-4	45.0	10
AACGCTA	20	7.0273364E-4	45.0	43
GTTCCGC	25	3.8858732E-5	45.0	13
GATGCGC	20	7.0273364E-4	45.0	12
ACGCTAC	20	7.0273364E-4	45.0	44
TTCGGTA	20	7.0273364E-4	45.0	25
TACTAGG	25	3.8858732E-5	45.0	2
CCGAACA	20	7.0273364E-4	45.0	39
>>END_MODULE