##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935944.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311197
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10201255153488	33.0	31.0	34.0	30.0	34.0
2	32.21428869815583	33.0	31.0	34.0	30.0	34.0
3	32.28645199021842	34.0	31.0	34.0	30.0	34.0
4	35.841679065029545	37.0	35.0	37.0	35.0	37.0
5	35.863411279671716	37.0	35.0	37.0	35.0	37.0
6	35.88200721729323	37.0	35.0	37.0	35.0	37.0
7	36.04249719630973	37.0	35.0	37.0	35.0	37.0
8	35.64521187543582	37.0	35.0	37.0	33.0	37.0
9	37.43709290256654	39.0	37.0	39.0	34.0	39.0
10	37.24003444763285	39.0	37.0	39.0	34.0	39.0
11	37.201293714271024	39.0	37.0	39.0	34.0	39.0
12	37.09535117626455	39.0	37.0	39.0	34.0	39.0
13	36.99935410688406	39.0	37.0	39.0	33.0	39.0
14	38.14101357018223	40.0	37.0	41.0	33.0	41.0
15	38.29096038843562	40.0	37.0	41.0	34.0	41.0
16	38.42998165149407	40.0	37.0	41.0	34.0	41.0
17	38.38852238292785	40.0	37.0	41.0	34.0	41.0
18	38.39991388091787	40.0	37.0	41.0	34.0	41.0
19	38.41957024007301	40.0	37.0	41.0	34.0	41.0
20	38.20989598228775	40.0	37.0	41.0	34.0	41.0
21	38.21546158864	40.0	37.0	41.0	34.0	41.0
22	38.217010446758806	40.0	37.0	41.0	34.0	41.0
23	38.22177913026154	40.0	37.0	41.0	34.0	41.0
24	38.13556364617911	40.0	37.0	41.0	34.0	41.0
25	38.083262370781206	40.0	36.0	41.0	34.0	41.0
26	37.90308711202229	40.0	36.0	41.0	34.0	41.0
27	37.82445524860458	40.0	36.0	41.0	33.0	41.0
28	37.82159211046379	40.0	36.0	41.0	33.0	41.0
29	37.81821482854912	40.0	36.0	41.0	33.0	41.0
30	37.742208311776785	40.0	36.0	41.0	33.0	41.0
31	37.78789641288316	40.0	36.0	41.0	33.0	41.0
32	37.672827822890326	40.0	36.0	41.0	33.0	41.0
33	37.59793956882618	40.0	36.0	41.0	33.0	41.0
34	37.5449538395293	40.0	36.0	41.0	33.0	41.0
35	37.536187688184654	40.0	36.0	41.0	33.0	41.0
36	37.48484079216702	40.0	35.0	41.0	33.0	41.0
37	37.42978242078169	40.0	35.0	41.0	33.0	41.0
38	37.33887537476261	40.0	35.0	41.0	33.0	41.0
39	37.3017059933097	40.0	35.0	41.0	33.0	41.0
40	37.165965610208325	39.0	35.0	41.0	32.0	41.0
41	37.07912672680006	39.0	35.0	41.0	32.0	41.0
42	37.06715681706443	39.0	35.0	41.0	32.0	41.0
43	36.988695263771824	39.0	35.0	41.0	32.0	41.0
44	36.93070948627397	39.0	35.0	41.0	32.0	41.0
45	36.8082050919514	39.0	35.0	41.0	31.0	41.0
46	36.70504535712105	39.0	35.0	41.0	31.0	41.0
47	36.60141325269845	39.0	35.0	41.0	31.0	41.0
48	36.567370508070454	39.0	35.0	41.0	31.0	41.0
49	36.565015086906364	39.0	35.0	41.0	31.0	41.0
50	36.375119297422536	38.0	35.0	40.0	31.0	41.0
51	35.31905834567814	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	8.0
11	7.0
12	4.0
13	6.0
14	6.0
15	4.0
16	18.0
17	30.0
18	54.0
19	121.0
20	241.0
21	404.0
22	599.0
23	832.0
24	1182.0
25	1443.0
26	1926.0
27	2314.0
28	2688.0
29	3175.0
30	4074.0
31	5292.0
32	7113.0
33	9796.0
34	18948.0
35	27006.0
36	21362.0
37	32200.0
38	59803.0
39	110426.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76811473118314	20.99923842453494	23.012432639132125	10.220214205149793
2	30.97105691892917	27.227768905227233	24.47613569539552	17.325038480448075
3	32.21978360973917	26.78303454082141	24.51212575956709	16.485056089872334
4	29.17187505020935	24.757950751453258	26.549741803423554	19.520432394913833
5	28.619170493288813	28.039473388239607	23.236727860487086	20.10462825798449
6	26.136820085026525	35.85349473163302	22.89032349283573	15.119361690504729
7	77.81566017667265	6.128593784644454	10.896955947518775	5.158790091164118
8	76.24495094747057	8.441919427243835	9.234022178877046	6.079107446408545
9	69.86764011221189	8.346160149358766	11.458015340764852	10.3281843976645
10	40.5193494795901	27.14229250281976	18.34015109400155	13.998206923588594
11	33.07743969254202	23.814175586525575	24.284938479484055	18.823446241448345
12	31.152613939080386	20.498912264578387	27.9263617579861	20.422112038355124
13	25.906098066498068	24.563540136955048	29.26474226936635	20.26561952718053
14	19.731231342204456	30.70209545721842	27.14293518253711	22.42373801804002
15	18.386745373509385	25.899349929465902	35.78729872074602	19.92660597627869
16	19.917287120377125	25.408342625410913	32.46721530091871	22.20715495329325
17	20.489593408676818	26.15738583598171	28.343782234404575	25.0092385209369
18	21.4651812196133	26.547813764271506	29.102144300876937	22.884860715238258
19	23.913148262997392	27.343451254350136	26.185342403686413	22.558058078966056
20	27.44338795039798	26.398712069846432	27.724238986879694	18.433660992875893
21	23.94014081112607	27.924755058692725	27.55746360022751	20.577640529953694
22	21.280089461016654	25.520490236088396	27.606307258746064	25.593113044148886
23	22.560628797835456	27.089271426138428	27.81807022561271	22.532029550413405
24	22.557415399248708	23.89933064907438	28.755739933225577	24.787514018451333
25	20.672757128121415	29.151630639112845	26.693702060109835	23.48191017265591
26	19.5564224590854	29.193726160599237	27.87334068130477	23.376510699010595
27	22.931133654887418	29.46911441948348	26.289456517897026	21.31029540773208
28	19.106546656940782	29.170589690774655	29.337365077426842	22.385498574857728
29	21.56865265410656	28.871100942489807	26.41156566419342	23.14868073921021
30	21.5872903659097	29.121103352538746	27.299749033570375	21.991857247981184
31	25.062580937476902	29.59989974196409	22.78331731989704	22.55420200066196
32	25.898064570031202	30.75800859262782	23.39097099265096	19.95295584469002
33	23.624585069907486	28.68247444544774	24.98417401196027	22.708766472684506
34	24.43243347461576	27.507977261991602	25.68855098217528	22.371038281217366
35	23.246689396106003	27.802003232678977	24.617846573071077	24.333460798143943
36	23.50568932219784	29.966227180853284	24.181145705132117	22.34693779181676
37	24.84021375527399	29.507032522807098	26.16831139117665	19.48444233074226
38	22.969694437928386	27.73741392108536	25.122671491049076	24.170220149937176
39	24.70139493632651	25.184047404055953	26.64228768272188	23.472269976895667
40	24.77626712339772	23.894831891052934	29.85375823031713	21.475142755232216
41	21.364280503989434	26.567736835509344	26.35500984906667	25.712972811434557
42	24.026581233109574	25.37267390109802	27.008293781752396	23.592451084040015
43	23.66346719280713	26.521785235718852	25.6361725852113	24.17857498626272
44	22.76532228781126	26.34922573161052	26.77917846251731	24.106273518060906
45	22.54681118391244	25.28848285812524	25.65673833616648	26.507967621795842
46	24.07317551261741	27.91479352307381	25.326079621590182	22.6859513427186
47	21.485104290851133	25.34793073198006	30.290780438114762	22.87618453905404
48	22.29102465640736	24.446572428397445	27.7470541168456	25.515348798349596
49	22.528173472109305	23.655112356481585	30.143606782841736	23.67310738856737
50	20.77365784374528	23.911862903562696	29.860827707207978	25.453651545484053
51	21.031372410402412	23.354338248762037	26.42538327811643	29.188906062719113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	319.5
2	438.0
3	646.5
4	855.0
5	622.5
6	390.0
7	403.5
8	417.0
9	416.0
10	415.0
11	423.5
12	432.0
13	467.5
14	503.0
15	545.0
16	587.0
17	579.0
18	571.0
19	607.0
20	643.0
21	702.5
22	762.0
23	871.5
24	981.0
25	1350.0
26	2099.5
27	2480.0
28	2912.0
29	3344.0
30	3785.5
31	4227.0
32	4693.5
33	5160.0
34	6371.5
35	7583.0
36	8107.5
37	8632.0
38	9205.5
39	9779.0
40	11524.0
41	13269.0
42	15435.5
43	17602.0
44	20253.5
45	22905.0
46	28674.5
47	34444.0
48	33969.0
49	33494.0
50	32683.5
51	31873.0
52	27805.0
53	23737.0
54	20428.0
55	17119.0
56	15380.0
57	13641.0
58	13713.5
59	13786.0
60	12713.5
61	11641.0
62	11108.5
63	10576.0
64	8488.0
65	6400.0
66	5297.5
67	4195.0
68	3415.0
69	2635.0
70	2129.5
71	1624.0
72	1330.5
73	1037.0
74	747.0
75	379.5
76	302.0
77	205.0
78	108.0
79	127.0
80	146.0
81	95.5
82	45.0
83	37.5
84	30.0
85	19.0
86	8.0
87	5.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	311197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.38845533029546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39788660364987	23.12467260939814
2	11.606981565429898	7.518644079029782
3	4.211149406798312	4.09177873353862
4	2.1659992363594114	2.8061347804912344
5	1.223998106422933	1.9821703997122697
6	0.8229986940176335	1.599339386284897
7	0.6349989915038506	1.4396645529773593
8	0.5099991900041589	1.3214468787149254
9	0.4869992265326655	1.4195837424997506
>10	6.773989241339032	46.925615092019946
>50	0.11299982053016099	2.3896164390058474
>100	0.04599992694148146	2.657792423215232
>500	0.0019999968235426724	0.48355887007803333
>1k	0.003999993647085345	2.239982013033944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	2084	0.6696722654781376	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	1767	0.5678075302782482	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	1640	0.5269973682265574	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1425	0.4579092986114905	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	808	0.25964260580918197	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	685	0.22011780319219015	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT	452	0.14524561612097803	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	394	0.1266079043178437	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	388	0.1246798651657953	No Hit
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	381	0.12243048615507218	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	327	0.10507813378663677	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.254822507929061	0.0
2	0.0	0.0	0.0	1.3297043351960334	0.0
3	0.0	0.0	0.0	1.8261744168484915	0.0
4	0.0	0.0	0.0	2.5408342625410913	0.0
5	0.0	0.0	0.0	4.81656314167553	0.0
6	0.0	0.0	0.0	5.716957425682124	0.0
7	0.0	0.0	0.0	6.706041510682944	0.0
8	0.0	0.0	0.0	8.287354955221291	0.0
9	0.0	0.0	0.0	8.804712127687607	0.0
10	0.0	0.0	0.0	10.565975893083802	0.0
11	0.0	0.0	0.0	12.218948126106614	0.0
12	0.0	0.0	0.0	14.23631975886657	0.0
13	3.2133985867473014E-4	0.0	0.0	14.8552203266741	0.0
14	3.2133985867473014E-4	0.0	0.0	15.105222736723041	0.0
15	3.2133985867473014E-4	0.0	0.0	15.648929777600683	0.0
16	3.2133985867473014E-4	0.0	0.0	16.60170245857126	0.0
17	3.2133985867473014E-4	0.0	0.0	17.640272881807988	0.0
18	3.2133985867473014E-4	0.0	0.0	18.834693136501958	0.0
19	6.426797173494603E-4	0.0	0.0	19.65539513555722	0.0
20	6.426797173494603E-4	0.0	0.0	20.33020883877415	0.0
21	6.426797173494603E-4	0.0	0.0	21.088570905246517	0.0
22	6.426797173494603E-4	0.0	0.0	21.991214568263832	0.0
23	6.426797173494603E-4	0.0	0.0	22.830232939263553	0.0
24	6.426797173494603E-4	0.0	0.0	23.497977165589642	0.0
25	6.426797173494603E-4	0.0	0.0	24.104345478908858	0.0
26	6.426797173494603E-4	0.0	0.0	24.611419775897584	0.0
27	6.426797173494603E-4	0.0	0.0	25.13423972596137	0.0
28	6.426797173494603E-4	0.0	0.0	25.660594414470577	0.0
29	6.426797173494603E-4	0.0	0.0	26.219083088847256	0.0
30	6.426797173494603E-4	0.0	0.0	26.855978688740574	0.0
31	6.426797173494603E-4	0.0	0.0	27.49158892919919	0.0
32	9.640195760241905E-4	0.0	0.0	28.06357387764021	0.0
33	9.640195760241905E-4	0.0	0.0	28.62334791145159	0.0
34	9.640195760241905E-4	0.0	0.0	29.165126913177183	0.0
35	9.640195760241905E-4	0.0	0.0	29.741289279780975	0.0
36	9.640195760241905E-4	0.0	0.0	30.328055861721033	0.0
37	9.640195760241905E-4	0.0	0.0	30.90261152903145	0.0
38	9.640195760241905E-4	0.0	0.0	31.45435206637596	0.0
39	9.640195760241905E-4	0.0	0.0	32.010912701600596	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCGA	30	2.160381E-6	45.000004	41
GTAAGTA	30	2.160381E-6	45.000004	10
GTCGCTA	20	7.025349E-4	45.0	20
GGTACAT	20	7.025349E-4	45.0	8
GTCGAAG	20	7.025349E-4	45.0	1
ATAGCGG	45	3.8198777E-10	45.0	2
AGGCGTC	35	1.2081182E-7	45.0	35
CGACAGG	20	7.025349E-4	45.0	2
ACGGGTA	25	3.884227E-5	45.0	5
CCCGTCT	20	7.025349E-4	45.0	40
TCGGAGT	20	7.025349E-4	45.0	27
ACCTACG	20	7.025349E-4	45.0	35
TACGAAT	80	0.0	45.0	12
CGCTAGG	20	7.025349E-4	45.0	2
TGCGAAG	20	7.025349E-4	45.0	1
TCGCTAT	20	7.025349E-4	45.0	21
CAGGCGT	35	1.2081182E-7	45.0	34
CGAATAT	80	0.0	45.0	14
CTCGCGG	20	7.025349E-4	45.0	2
TTAACGG	20	7.025349E-4	45.0	2
>>END_MODULE