##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935943.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	523003
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19006583136235	33.0	31.0	34.0	30.0	34.0
2	32.29514935860789	34.0	31.0	34.0	30.0	34.0
3	32.3473421758575	34.0	31.0	34.0	30.0	34.0
4	35.88153414033954	37.0	35.0	37.0	35.0	37.0
5	35.919119010789615	37.0	35.0	37.0	35.0	37.0
6	35.93531394657392	37.0	35.0	37.0	35.0	37.0
7	36.08689051496837	37.0	35.0	37.0	35.0	37.0
8	35.795450504108004	37.0	35.0	37.0	35.0	37.0
9	37.54481140643553	39.0	37.0	39.0	35.0	39.0
10	37.26584933547226	39.0	37.0	39.0	34.0	39.0
11	37.220981524006554	39.0	37.0	39.0	34.0	39.0
12	37.15212149834704	39.0	37.0	39.0	34.0	39.0
13	37.1610583495697	39.0	37.0	39.0	34.0	39.0
14	38.385976753479426	40.0	38.0	41.0	34.0	41.0
15	38.525408076053104	40.0	38.0	41.0	34.0	41.0
16	38.60260457396994	40.0	38.0	41.0	35.0	41.0
17	38.57487624353971	40.0	38.0	41.0	34.0	41.0
18	38.54432957363533	40.0	38.0	41.0	34.0	41.0
19	38.5335992336564	40.0	38.0	41.0	34.0	41.0
20	38.4195960635025	40.0	37.0	41.0	34.0	41.0
21	38.397087588407715	40.0	37.0	41.0	34.0	41.0
22	38.398420276747935	40.0	37.0	41.0	34.0	41.0
23	38.38558287428562	40.0	37.0	41.0	34.0	41.0
24	38.31170758102726	40.0	37.0	41.0	34.0	41.0
25	38.22613828218959	40.0	37.0	41.0	34.0	41.0
26	38.0873933801527	40.0	37.0	41.0	34.0	41.0
27	38.042020791467735	40.0	37.0	41.0	34.0	41.0
28	38.0466001151045	40.0	37.0	41.0	34.0	41.0
29	38.01646453270823	40.0	37.0	41.0	34.0	41.0
30	37.92447653263939	40.0	36.0	41.0	34.0	41.0
31	37.95302130198106	40.0	37.0	41.0	34.0	41.0
32	37.87895671726548	40.0	36.0	41.0	34.0	41.0
33	37.84982877727279	40.0	36.0	41.0	33.0	41.0
34	37.8084466054688	40.0	36.0	41.0	33.0	41.0
35	37.763379942371266	40.0	36.0	41.0	33.0	41.0
36	37.68566719502565	40.0	36.0	41.0	33.0	41.0
37	37.65419701225423	40.0	36.0	41.0	33.0	41.0
38	37.56716309466676	40.0	36.0	41.0	33.0	41.0
39	37.52443867434795	40.0	36.0	41.0	33.0	41.0
40	37.42903769194441	40.0	35.0	41.0	33.0	41.0
41	37.33091205977786	40.0	35.0	41.0	33.0	41.0
42	37.30456421856089	40.0	35.0	41.0	33.0	41.0
43	37.24187050552291	39.0	35.0	41.0	33.0	41.0
44	37.1584503339369	39.0	35.0	41.0	33.0	41.0
45	37.050670837452174	39.0	35.0	41.0	32.0	41.0
46	36.97686246541607	39.0	35.0	41.0	32.0	41.0
47	36.92789333904395	39.0	35.0	41.0	32.0	41.0
48	36.838434961176134	39.0	35.0	41.0	32.0	41.0
49	36.805939927686836	39.0	35.0	41.0	32.0	41.0
50	36.67259078819816	39.0	35.0	41.0	31.0	41.0
51	35.71779320577511	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	14.0
11	13.0
12	10.0
13	12.0
14	15.0
15	22.0
16	30.0
17	61.0
18	95.0
19	173.0
20	280.0
21	452.0
22	716.0
23	1030.0
24	1435.0
25	2137.0
26	2864.0
27	3510.0
28	4206.0
29	5067.0
30	6419.0
31	8140.0
32	10642.0
33	15495.0
34	29871.0
35	39851.0
36	36387.0
37	55619.0
38	103282.0
39	194955.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87909820784967	21.264505174922515	23.84498750485179	11.011409112376029
2	32.88508861325843	24.37175312569909	24.914962246870477	17.828196014172004
3	33.482981933182025	24.41840677778139	25.029493138662684	17.0691181503739
4	29.711110643724798	25.85166815486718	25.41648900675522	19.020732194652805
5	26.313615791878824	30.02793483020174	23.36143387322826	20.297015504691178
6	26.163712254040604	35.24511331674962	23.251491865247427	15.339682563962349
7	80.01445498400582	6.73973189446332	8.041445268956391	5.204367852574459
8	80.04466513576403	6.0783590151490525	8.17547891694694	5.701496932139968
9	73.65521803890226	8.912185972164597	10.506823096617037	6.925772892316105
10	40.16286713460534	26.902331344179668	17.763378030336348	15.171423490878638
11	30.17936799597708	25.094119919006204	24.891635420829324	19.834876664187394
12	27.753186884205256	23.148050775999373	28.37077416381933	20.727988175976044
13	24.47825347082139	24.124335806869176	30.456613059580924	20.94079766272851
14	21.138693277094013	27.022024730259673	29.422966981068942	22.41631501157737
15	20.323975197083	26.1273835905339	32.439010866094456	21.109630346288643
16	22.942698225440388	25.240390590493746	30.243421165844175	21.57349001822169
17	22.52855146146389	25.740961332917784	27.915709852524746	23.81477735309358
18	23.693936746060732	26.010175849851723	28.417045408917346	21.878841995170202
19	23.67768444922878	27.384164144373933	26.766768068252	22.171383338145287
20	25.970979133962903	26.613614071047394	27.308447561486265	20.106959233503442
21	25.234080875253106	26.794301371120245	28.005384290338682	19.966233463287974
22	23.66181455938111	24.377871637447587	27.56064496761969	24.399668835551612
23	22.0362024692019	25.95721248252878	28.7174260950702	23.28915895319912
24	23.222237730949917	24.72452356869846	28.977271640889253	23.075967059462375
25	22.552260694489323	26.530822958950523	26.996977072789257	23.919939273770897
26	21.390699479735297	28.042095360829673	27.19276944874121	23.37443571069382
27	22.335627137894047	27.243820781142748	27.725271174352727	22.695280906610478
28	20.677319250558792	27.403093290095846	28.466184706397478	23.45340275294788
29	21.653030670952177	26.475756353214035	27.636170347015216	24.235042628818572
30	22.850920549212912	26.150519213082905	26.936747972764973	24.061812264939206
31	24.182270464987774	27.37020628944767	24.976529771339745	23.470993474224812
32	23.81496855658572	28.669625222035055	24.532364059097176	22.983042162282054
33	23.02243008166301	27.702709162280144	24.027969246830324	25.246891509226526
34	22.207903205144138	27.86217287472538	26.344781961097734	23.58514195903274
35	22.61382821895859	27.70385638323298	25.202341095557767	24.479974302250657
36	22.289738299780307	29.907859037137452	24.556264495614748	23.24613816746749
37	22.97673244704141	29.538262686829714	25.234080875253106	22.25092399087577
38	22.702546639311823	29.40900912614268	24.36181054410778	23.526633690437723
39	24.129115894172692	26.30309959981109	25.41323854738883	24.154545958627388
40	24.486092813999154	25.484939856941548	27.096402888702357	22.93256444035694
41	20.91919166811663	28.001177813511585	26.156828928323545	24.92280159004824
42	21.836777226899272	28.33253346539121	25.88455515551536	23.94613415219416
43	22.48782511763795	26.63254321676931	26.966958124523188	23.912673541069555
44	22.206373577207014	26.31227736743384	25.977480052695682	25.503869002663464
45	23.195660445542376	24.940201107833033	25.785702950078683	26.07843549654591
46	23.190115544270302	26.785506010481775	25.865243602809162	24.15913484243876
47	22.141173186387075	26.84038141272612	27.698693888945186	23.319751511941615
48	21.458003108968782	26.280728791230644	27.708445267044358	24.55282283275622
49	22.638684672936865	24.811712361114562	28.571155423582656	23.978447542365913
50	21.634483932214536	24.734274946797626	28.300602482203736	25.330638638784098
51	21.1866853536213	24.132748760523363	26.848603162888168	27.831962722967173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	535.5
2	845.0
3	1209.5
4	1574.0
5	1146.5
6	719.0
7	728.5
8	738.0
9	770.0
10	802.0
11	809.0
12	816.0
13	839.0
14	862.0
15	874.5
16	887.0
17	921.0
18	955.0
19	972.5
20	990.0
21	1131.5
22	1273.0
23	1523.0
24	1773.0
25	2191.0
26	3241.5
27	3874.0
28	5104.0
29	6334.0
30	7279.0
31	8224.0
32	9091.0
33	9958.0
34	11700.0
35	13442.0
36	14507.5
37	15573.0
38	17434.5
39	19296.0
40	21062.0
41	22828.0
42	25044.5
43	27261.0
44	30949.5
45	34638.0
46	38250.0
47	41862.0
48	47121.5
49	52381.0
50	51631.5
51	50882.0
52	45536.5
53	40191.0
54	35771.5
55	31352.0
56	28979.0
57	26606.0
58	26023.5
59	25441.0
60	23400.0
61	21359.0
62	19580.5
63	17802.0
64	15629.0
65	13456.0
66	11065.5
67	8675.0
68	7313.0
69	5951.0
70	5224.0
71	4497.0
72	3517.0
73	2537.0
74	2220.5
75	1340.0
76	776.0
77	621.0
78	466.0
79	332.0
80	198.0
81	165.5
82	133.0
83	80.0
84	27.0
85	15.0
86	3.0
87	3.0
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	523003.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.69822494392241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97535839697555	21.788175175458676
2	11.03268482449313	6.773676809553783
3	3.9779734490635392	3.6635017128091016
4	2.0613285831636206	2.5311651411717477
5	1.220439425679271	1.8732662009966865
6	0.9252463670668282	1.7042052662858698
7	0.7117522372353285	1.52946711980931
8	0.5852537348429364	1.4373000641183344
9	0.5497456942764269	1.5188595286365496
>10	7.7877011315505	51.13972586105528
>50	0.12151685416422423	2.4233756430381783
>100	0.04785119645845099	2.6883659131454793
>500	0.0018888630180967498	0.42154907301533473
>1k	0.0012592420120644998	0.5073664909056643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1490	0.2848932032894649	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1135	0.21701596357955882	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	881	0.16845027657585138	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.12734152576562657	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	634	0.12122301401712801	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9120349214058046E-4	0.0	0.0	0.17552480578505286	0.0
2	1.9120349214058046E-4	0.0	0.0	0.8694022787632193	0.0
3	1.9120349214058046E-4	0.0	0.0	1.2521916700286615	0.0
4	1.9120349214058046E-4	0.0	0.0	1.757351296264075	0.0
5	1.9120349214058046E-4	0.0	0.0	3.0969229622009817	0.0
6	1.9120349214058046E-4	0.0	0.0	3.892520692997937	0.0
7	1.9120349214058046E-4	0.0	0.0	4.692516104114126	0.0
8	1.9120349214058046E-4	0.0	0.0	6.017365101156208	0.0
9	1.9120349214058046E-4	0.0	0.0	6.530364070569385	0.0
10	1.9120349214058046E-4	0.0	0.0	7.620415179262834	0.0
11	1.9120349214058046E-4	0.0	0.0	9.136467668445496	0.0
12	1.9120349214058046E-4	0.0	0.0	10.326518203528469	0.0
13	3.8240698428116093E-4	0.0	0.0	10.821735248172573	0.0
14	3.8240698428116093E-4	0.0	0.0	11.016380403171684	0.0
15	3.8240698428116093E-4	0.0	0.0	11.376034171888115	0.0
16	3.8240698428116093E-4	0.0	0.0	12.140465733466156	0.0
17	3.8240698428116093E-4	0.0	0.0	13.050211949071038	0.0
18	5.736104764217414E-4	0.0	0.0	14.000684508501863	0.0
19	5.736104764217414E-4	0.0	0.0	14.572382950002199	0.0
20	5.736104764217414E-4	0.0	0.0	15.15937767087378	0.0
21	5.736104764217414E-4	0.0	0.0	15.848857463532713	0.0
22	5.736104764217414E-4	0.0	0.0	16.59569830383382	0.0
23	5.736104764217414E-4	0.0	0.0	17.344068772072053	0.0
24	5.736104764217414E-4	0.0	0.0	17.910222312300313	0.0
25	5.736104764217414E-4	0.0	0.0	18.435267101718345	0.0
26	5.736104764217414E-4	0.0	0.0	18.89721473872999	0.0
27	5.736104764217414E-4	0.0	0.0	19.38516605067275	0.0
28	7.648139685623219E-4	0.0	0.0	19.874838194044777	0.0
29	9.560174607029022E-4	0.0	0.0	20.39644132060428	0.0
30	9.560174607029022E-4	0.0	0.0	20.960491622418992	0.0
31	9.560174607029022E-4	0.0	0.0	21.494522975967634	0.0
32	9.560174607029022E-4	0.0	0.0	22.00752194538081	0.0
33	9.560174607029022E-4	0.0	0.0	22.50445982145418	0.0
34	9.560174607029022E-4	0.0	0.0	23.00216251149611	0.0
35	9.560174607029022E-4	0.0	0.0	23.524721655516316	0.0
36	0.0011472209528434827	0.0	0.0	24.01095213602981	0.0
37	0.0011472209528434827	0.0	0.0	24.518597407663055	0.0
38	0.0011472209528434827	0.0	0.0	25.02394823739061	0.0
39	0.0011472209528434827	0.0	0.0	25.542683311568002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTAT	20	7.029758E-4	45.0	23
ATAGCCG	20	7.029758E-4	45.0	1
CTACGAT	20	7.029758E-4	45.0	30
TTCGGTA	20	7.029758E-4	45.0	22
CGGTCTA	35	1.2102282E-7	45.0	31
ATAACGG	35	1.2102282E-7	45.0	2
GCGCTAG	25	3.8878796E-5	45.0	1
TATCGCG	20	7.029758E-4	45.0	32
AATCCGG	30	2.163224E-6	44.999996	2
TAACGGG	80	0.0	42.1875	3
CACGGGT	55	6.002665E-11	40.909092	4
ATAGCGG	55	6.002665E-11	40.909092	2
CGCGCAT	55	6.002665E-11	40.909092	36
CCCGATG	50	1.0786607E-9	40.5	15
CGTTCGG	45	1.9248546E-8	40.000004	2
GTACGAG	45	1.9248546E-8	40.000004	1
TCGACGT	40	3.453406E-7	39.375	26
CGTATGG	70	0.0	38.571426	2
TAGAACG	35	6.2417603E-6	38.571426	1
GCGATAA	35	6.2417603E-6	38.571426	9
>>END_MODULE