##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935941.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426120
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99392189993429	33.0	31.0	34.0	30.0	34.0
2	32.12095419130761	33.0	31.0	34.0	30.0	34.0
3	32.20267060921806	34.0	31.0	34.0	30.0	34.0
4	35.79955646296818	37.0	35.0	37.0	35.0	37.0
5	35.77103867455177	37.0	35.0	37.0	35.0	37.0
6	35.7765230451516	37.0	35.0	37.0	35.0	37.0
7	35.91341171500986	37.0	35.0	37.0	35.0	37.0
8	35.28224913169999	37.0	35.0	37.0	32.0	37.0
9	37.175722801088895	39.0	37.0	39.0	34.0	39.0
10	37.03173753872149	39.0	37.0	39.0	33.0	39.0
11	37.02403078944898	39.0	37.0	39.0	33.0	39.0
12	36.94258894208204	39.0	37.0	39.0	33.0	39.0
13	36.83822632122407	39.0	36.0	39.0	33.0	39.0
14	38.05398244625927	40.0	37.0	41.0	33.0	41.0
15	38.08285459494978	40.0	37.0	41.0	33.0	41.0
16	38.35851403360556	40.0	37.0	41.0	34.0	41.0
17	38.138315497981786	40.0	37.0	41.0	33.0	41.0
18	38.20068525298038	40.0	37.0	41.0	34.0	41.0
19	38.22743593353985	40.0	37.0	41.0	34.0	41.0
20	37.75058199568197	40.0	36.0	41.0	32.0	41.0
21	37.996299164554586	40.0	37.0	41.0	33.0	41.0
22	37.85851403360556	40.0	37.0	41.0	33.0	41.0
23	37.70441424950718	40.0	36.0	41.0	33.0	41.0
24	37.72720360461842	40.0	36.0	41.0	33.0	41.0
25	37.877119121374264	40.0	36.0	41.0	33.0	41.0
26	37.55220125786163	40.0	36.0	41.0	32.0	41.0
27	37.38101473763259	40.0	36.0	41.0	32.0	41.0
28	37.37100581995682	40.0	36.0	41.0	32.0	41.0
29	37.295818079414246	40.0	36.0	41.0	31.0	41.0
30	37.322477236459214	40.0	36.0	41.0	32.0	41.0
31	37.417046841265375	40.0	36.0	41.0	32.0	41.0
32	37.1084952595513	40.0	36.0	41.0	31.0	41.0
33	36.97538956162583	40.0	35.0	41.0	31.0	41.0
34	36.77621092649958	40.0	35.0	41.0	30.0	41.0
35	36.67710034732001	40.0	35.0	41.0	30.0	41.0
36	36.77014690697456	40.0	35.0	41.0	30.0	41.0
37	36.52939782220971	40.0	35.0	41.0	30.0	41.0
38	36.482784192246314	40.0	35.0	41.0	30.0	41.0
39	36.391718295315876	40.0	35.0	41.0	30.0	41.0
40	36.32742654651272	39.0	35.0	41.0	29.0	41.0
41	36.31958368534685	39.0	35.0	41.0	29.0	41.0
42	36.313463343659066	39.0	35.0	41.0	30.0	41.0
43	36.22563831784474	39.0	35.0	41.0	30.0	41.0
44	36.34165962639632	39.0	35.0	41.0	30.0	41.0
45	36.279634844644704	39.0	35.0	41.0	30.0	41.0
46	36.210391439031255	39.0	35.0	41.0	29.0	41.0
47	36.069931005350604	39.0	35.0	41.0	29.0	41.0
48	36.00585046465784	39.0	35.0	41.0	29.0	41.0
49	36.026893832723175	39.0	35.0	41.0	29.0	41.0
50	35.86408288744954	39.0	35.0	41.0	28.0	41.0
51	35.015063831784474	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	9.0
11	9.0
12	4.0
13	6.0
14	7.0
15	12.0
16	23.0
17	39.0
18	137.0
19	317.0
20	726.0
21	1768.0
22	2923.0
23	3702.0
24	4019.0
25	3985.0
26	4134.0
27	4117.0
28	4242.0
29	4617.0
30	5686.0
31	7688.0
32	10333.0
33	13737.0
34	23693.0
35	30792.0
36	28146.0
37	42285.0
38	77603.0
39	151211.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.84999530648644	18.60485309302544	21.051816389749366	9.493335210738758
2	27.89003097718952	35.684783629024686	20.734065521449356	15.691119872336431
3	27.77621327325636	36.01121749741857	21.384117150098565	14.828452079226508
4	26.036797146343755	21.22899652679996	36.01778841640852	16.716417910447763
5	34.72073594292687	25.1107669201164	20.60358584436309	19.56491129259364
6	23.080822303576458	41.361822960668356	21.758894208204264	13.798460527550924
7	70.60687130385807	5.247348164836197	19.415657561250352	4.730122970055383
8	68.34905660377359	16.149676147564065	9.577818454895334	5.9234487937670135
9	63.16131606120342	7.507040270346381	9.752651835163803	19.5789918332864
10	42.770346381301046	24.45203229137332	18.077302168403268	14.70031915892237
11	34.86881629587909	23.19956819675209	23.427672955974842	18.503942551393973
12	28.197456115648173	19.196001126443253	27.165117807190462	25.441424950718105
13	27.858115084952594	21.126208579742794	31.97690791326387	19.03876842204074
14	19.245283018867926	33.02543884351826	24.718154510466537	23.01112362714728
15	15.838730873932224	24.532760724678496	36.772036046184176	22.856472355205106
16	17.968647329390784	24.81437153853375	28.84563033887168	28.371350793203792
17	17.68445508307519	28.89702431240026	30.098563784849336	23.31995681967521
18	18.567304984511406	26.545104665352483	27.43968835069933	27.44790199943678
19	20.809161738477425	27.493429081010046	24.935464188491505	26.761944992021025
20	27.723645921336715	25.235614380925558	29.59494977940486	17.445789918332864
21	22.431240026283675	32.76025532713789	25.713179386088424	19.095325260490004
22	19.320848587252417	23.803623392471604	30.50807284333052	26.36745517694546
23	23.621045714822117	29.29878907350042	27.27729278137614	19.802872430301324
24	22.84473857129447	26.616446071529147	25.66577489908946	24.873040458086923
25	19.29785037078757	35.114991082324224	23.671735661316063	21.915422885572138
26	19.26663850558528	26.67136018023092	29.648690509715575	24.413310804468225
27	25.844597765887546	29.262649019055665	23.51309490284427	21.37965831221252
28	18.363606495822772	30.91523514502957	28.76020839200225	21.960949967145403
29	22.47301229700554	27.749929597296536	27.248897024312402	22.528161081385527
30	20.524500140805408	34.15094339622641	25.072280108889515	20.252276354078663
31	25.96967990237492	26.278747770581056	20.87393222566413	26.87764010137989
32	30.065943865577772	29.78691448418286	21.2167933915329	18.930348258706466
33	25.70801652116775	28.706467661691544	21.53712569229325	24.04839012484746
34	27.035342157138835	26.74786445132826	23.020745329954003	23.196048061578896
35	24.03689101661504	26.440908664226036	23.121421195907256	26.400779123251667
36	22.09213367126631	33.0780061954379	24.55904440063832	20.270815732657468
37	24.191307612879	28.79705247348165	26.220548202384304	20.791091711255046
38	24.22674364028912	26.377780906786818	22.722003191589224	26.673472261334837
39	28.958509340091993	25.16708908288745	24.695625645358117	21.178775931662443
40	21.362526987702996	26.16891955317751	27.559842297944243	24.908711161175255
41	21.81263493851497	26.364639068806913	26.80113583028255	25.02159016239557
42	21.362057636346567	24.748662348634188	28.694029850746265	25.195250164272977
43	23.670092931568572	26.822256641321694	26.83727588472731	22.670374542382426
44	23.564958227729278	25.096686379423637	27.249366375668828	24.088989017178257
45	22.40472167464564	22.772223786726745	25.241246597202665	29.581807941424948
46	27.483103351168687	27.94517976156951	23.20731249413311	21.364404393128698
47	19.52642448136675	27.04097437341594	32.64714165023937	20.78545949497794
48	23.362198441753495	25.124847460809164	26.04430676804656	25.46864732939078
49	21.534778935511124	22.244672862104572	32.12968177978034	24.090866422603963
50	22.20900215901624	23.460058199568195	28.980334178165773	25.350605463249785
51	20.65122500704027	22.993288275603117	25.62611470947151	30.729372007885104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	322.0
2	495.0
3	876.0
4	1257.0
5	914.5
6	572.0
7	560.5
8	549.0
9	532.5
10	516.0
11	552.0
12	588.0
13	552.0
14	516.0
15	565.5
16	615.0
17	693.0
18	771.0
19	751.5
20	732.0
21	980.0
22	1228.0
23	1261.5
24	1295.0
25	1819.0
26	3121.5
27	3900.0
28	4230.5
29	4561.0
30	5188.0
31	5815.0
32	6702.5
33	7590.0
34	8715.5
35	9841.0
36	11027.0
37	12213.0
38	13391.5
39	14570.0
40	15255.5
41	15941.0
42	18629.5
43	21318.0
44	22855.0
45	24392.0
46	45661.0
47	66930.0
48	54079.0
49	41228.0
50	39909.5
51	38591.0
52	34160.0
53	29729.0
54	26849.0
55	23969.0
56	22695.5
57	21422.0
58	20253.0
59	19084.0
60	18885.5
61	18687.0
62	16171.5
63	13656.0
64	11257.0
65	8858.0
66	6862.0
67	4866.0
68	3914.0
69	2962.0
70	2478.0
71	1994.0
72	1596.5
73	1199.0
74	904.5
75	428.5
76	247.0
77	191.5
78	136.0
79	124.5
80	113.0
81	82.5
82	52.0
83	35.0
84	18.0
85	9.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.44212625908429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38575781056896	22.035763786288374
2	10.004604123296973	6.091228437871235
3	3.5977842211362185	3.2857260453831008
4	1.8400906114418125	2.2406508288666913
5	1.269738846351546	1.9326775138348895
6	0.9817034143825719	1.7931083573765039
7	0.7978956012589293	1.700274704456461
8	0.7480705746247556	1.8218287106746038
9	0.7037639026110244	1.9281662621883637
>10	7.526888765162089	42.59181250585542
>50	0.0869980457648277	1.7642636952458386
>100	0.0473828642112008	2.816059019793954
>500	0.00233030479727217	0.4935019879343339
>1k	0.00466060959454434	2.5531101886569254
>5k	0.0015535365315147802	4.42401949797003
>10k+	7.767682657573901E-4	2.5278084576032724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	10690	2.5086830000938702	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	9474	2.223317375387215	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	9235	2.1672298882943775	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2536	0.5951375199474327	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT	2070	0.4857786539003098	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	1913	0.4489345724209143	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTA	1507	0.353656247066554	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC	1394	0.3271378954285178	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	1377	0.32314840889890173	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	769	0.180465596545574	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	709	0.1663850558528114	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	609	0.14291748803154042	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATG	497	0.1166338120717169	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	476	0.11170562282924999	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.040270346381301E-4	0.0	0.0	0.17600675865953253	0.0
2	7.040270346381301E-4	0.0	0.0	1.1367689852623675	0.0
3	7.040270346381301E-4	0.0	0.0	1.462498826621609	0.0
4	7.040270346381301E-4	0.0	0.0	2.216746456397259	0.0
5	7.040270346381301E-4	0.0	0.0	5.664836196376608	0.0
6	7.040270346381301E-4	0.0	0.0	6.415094339622642	0.0
7	7.040270346381301E-4	0.0	0.0	7.507744297381019	0.0
8	7.040270346381301E-4	0.0	0.0	8.757157608185487	0.0
9	7.040270346381301E-4	0.0	0.0	9.103069557871022	0.0
10	7.040270346381301E-4	0.0	0.0	12.13460996902281	0.0
11	7.040270346381301E-4	0.0	0.0	13.363841171500985	0.0
12	7.040270346381301E-4	0.0	0.0	16.952970994086172	0.0
13	7.040270346381301E-4	0.0	0.0	17.469022810475924	0.0
14	7.040270346381301E-4	0.0	0.0	17.727400732188116	0.0
15	7.040270346381301E-4	0.0	0.0	18.408664226039612	0.0
16	7.040270346381301E-4	0.0	0.0	19.15329015300854	0.0
17	7.040270346381301E-4	0.0	0.0	19.906364404393127	0.0
18	7.040270346381301E-4	0.0	0.0	20.62681873650615	0.0
19	7.040270346381301E-4	0.0	0.0	21.64014831502863	0.0
20	9.387027128508401E-4	0.0	0.0	22.221674645639727	0.0
21	9.387027128508401E-4	0.0	0.0	22.81446540880503	0.0
22	9.387027128508401E-4	0.0	0.0	23.557917957382898	0.0
23	9.387027128508401E-4	0.0	0.0	24.204684126537124	0.0
24	9.387027128508401E-4	0.0	0.0	24.69867642917488	0.0
25	9.387027128508401E-4	0.0	0.0	25.12719421759129	0.0
26	9.387027128508401E-4	0.0	0.0	25.518163897493665	0.0
27	9.387027128508401E-4	0.0	0.0	26.010982821740356	0.0
28	9.387027128508401E-4	0.0	0.0	26.424716042429363	0.0
29	9.387027128508401E-4	0.0	0.0	26.82366469539097	0.0
30	0.0014080540692762602	0.0	0.0	27.319534403454426	0.0
31	0.0014080540692762602	0.0	0.0	27.75626584060828	0.0
32	0.0014080540692762602	0.0	0.0	28.199098845395664	0.0
33	0.0014080540692762602	0.0	0.0	28.621749741856753	0.0
34	0.0014080540692762602	0.0	0.0	29.02421852999155	0.0
35	0.0014080540692762602	0.0	0.0	29.458133859006853	0.0
36	0.0014080540692762602	0.0	0.0	29.89674270158641	0.0
37	0.0014080540692762602	0.0	0.0	30.364216652586126	0.0
38	0.0014080540692762602	0.0	0.0	30.786867549047216	0.0
39	0.0014080540692762602	0.0	0.0	31.20975312118652	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	60	0.0	45.000004	6
CGAACGC	60	0.0	45.000004	34
AATACGG	30	2.1622745E-6	45.000004	2
AACCGAT	25	3.8866594E-5	45.0	16
AGTCATT	20	7.028285E-4	45.0	40
GGTAAGT	25	3.8866594E-5	45.0	8
GAATGCG	20	7.028285E-4	45.0	1
CGTGCAT	20	7.028285E-4	45.0	38
TAGGTCG	20	7.028285E-4	45.0	1
CCGTGCA	20	7.028285E-4	45.0	37
TCGGATC	20	7.028285E-4	45.0	38
CCGTAAG	20	7.028285E-4	45.0	42
TTCGGAT	25	3.8866594E-5	45.0	37
ATAACGG	20	7.028285E-4	45.0	2
TGTCGCG	20	7.028285E-4	45.0	1
CCCATAC	25	3.8866594E-5	45.0	16
ATCGACG	25	3.8866594E-5	45.0	1
ATTTCCG	80	0.0	45.0	32
CGAATGC	255	0.0	44.11765	45
CGATGAA	1405	0.0	43.55872	19
>>END_MODULE