##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462519
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.126844518819766	33.0	31.0	34.0	30.0	34.0
2	32.237283225121566	34.0	31.0	34.0	30.0	34.0
3	32.28966810012129	34.0	31.0	34.0	30.0	34.0
4	35.85102882259972	37.0	35.0	37.0	35.0	37.0
5	35.8719295855954	37.0	35.0	37.0	35.0	37.0
6	35.89085421355663	37.0	35.0	37.0	35.0	37.0
7	36.03392293073366	37.0	35.0	37.0	35.0	37.0
8	35.66158147016663	37.0	35.0	37.0	35.0	37.0
9	37.394385960360545	39.0	37.0	39.0	34.0	39.0
10	37.22341136256024	39.0	37.0	39.0	34.0	39.0
11	37.20982921782673	39.0	37.0	39.0	34.0	39.0
12	37.13448312393653	39.0	37.0	39.0	34.0	39.0
13	37.12306737669155	39.0	37.0	39.0	34.0	39.0
14	38.33237337276955	40.0	38.0	41.0	34.0	41.0
15	38.44980854840558	40.0	38.0	41.0	34.0	41.0
16	38.54270635368493	40.0	38.0	41.0	34.0	41.0
17	38.49703687848499	40.0	38.0	41.0	34.0	41.0
18	38.48176831654484	40.0	38.0	41.0	34.0	41.0
19	38.50677918096338	40.0	38.0	41.0	34.0	41.0
20	38.356930201786305	40.0	37.0	41.0	34.0	41.0
21	38.34883107504773	40.0	37.0	41.0	34.0	41.0
22	38.32554338308264	40.0	37.0	41.0	34.0	41.0
23	38.337363438042544	40.0	37.0	41.0	34.0	41.0
24	38.24635960901065	40.0	37.0	41.0	34.0	41.0
25	38.157044359258755	40.0	37.0	41.0	34.0	41.0
26	38.01249029769588	40.0	37.0	41.0	34.0	41.0
27	37.96352582272296	40.0	37.0	41.0	33.0	41.0
28	37.91713853917353	40.0	37.0	41.0	33.0	41.0
29	37.93485456813666	40.0	37.0	41.0	33.0	41.0
30	37.8270211602118	40.0	36.0	41.0	33.0	41.0
31	37.850568300977905	40.0	36.0	41.0	33.0	41.0
32	37.821311124515965	40.0	36.0	41.0	33.0	41.0
33	37.783204581865824	40.0	36.0	41.0	33.0	41.0
34	37.73401092711867	40.0	36.0	41.0	33.0	41.0
35	37.71971746025569	40.0	36.0	41.0	33.0	41.0
36	37.646604788127625	40.0	36.0	41.0	33.0	41.0
37	37.61158568620965	40.0	36.0	41.0	33.0	41.0
38	37.53841031395467	40.0	36.0	41.0	33.0	41.0
39	37.46894073540763	40.0	36.0	41.0	33.0	41.0
40	37.36419909236161	40.0	35.0	41.0	33.0	41.0
41	37.27501789115691	40.0	35.0	41.0	32.0	41.0
42	37.23949070200359	40.0	35.0	41.0	32.0	41.0
43	37.16962762610833	40.0	35.0	41.0	32.0	41.0
44	37.066047016446895	39.0	35.0	41.0	32.0	41.0
45	36.951031200880394	39.0	35.0	41.0	32.0	41.0
46	36.86745193170443	39.0	35.0	41.0	31.0	41.0
47	36.799582287430354	39.0	35.0	41.0	31.0	41.0
48	36.72625989418813	39.0	35.0	41.0	31.0	41.0
49	36.65135486325967	39.0	35.0	41.0	31.0	41.0
50	36.48113050490899	39.0	35.0	41.0	31.0	41.0
51	35.47589612534836	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	7.0
10	8.0
11	11.0
12	8.0
13	13.0
14	13.0
15	13.0
16	31.0
17	43.0
18	85.0
19	184.0
20	279.0
21	519.0
22	771.0
23	1185.0
24	1514.0
25	2114.0
26	2810.0
27	3439.0
28	3971.0
29	4816.0
30	6101.0
31	7509.0
32	10066.0
33	13966.0
34	26488.0
35	34111.0
36	31806.0
37	48125.0
38	90713.0
39	171646.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85865661734977	20.427917555819327	23.056566324842873	10.656859501988027
2	32.1294908965902	25.419496280152813	23.789292980396482	18.66171984286051
3	32.44558601916894	26.112873200884724	24.485048181804423	16.956492598141914
4	29.656295200845804	24.660176122494427	25.63224429699104	20.051284379668726
5	26.89511133596674	29.519003543638206	23.091808120315058	20.494077000079997
6	25.923907990806867	35.906849232139656	23.33071722458969	14.838525552463791
7	78.22662420354624	6.918850901260272	9.456260175257665	5.398264719935829
8	77.36157865947129	7.797733714723071	8.698453468938574	6.142234156867069
9	70.53656174124738	9.553553475641001	11.379208205500747	8.530676577610866
10	38.83018859765761	28.290081056129583	17.90348072187305	14.976249624339758
11	30.879812504999794	26.664418110391143	23.406605998888693	19.04916338572037
12	29.629053076738472	22.646853426561936	27.814208713587984	19.90988478311161
13	26.064010343358866	24.595530129573056	28.902380226542046	20.43807930052603
14	20.32003009606092	29.32009279618783	27.059861324615852	23.300015783135393
15	19.011110894903776	27.298554221556305	33.18523130941648	20.505103574123442
16	21.32409695601694	27.23758375331608	30.64371409606957	20.79460519459741
17	21.435443733122312	26.560422382648063	27.4942218589939	24.50991202523572
18	22.926625716997574	27.398009595281493	28.09225134535014	21.5831133423708
19	25.191181335253255	26.79479113290481	26.186599901841873	21.827427630000066
20	27.053158897256115	26.19654543921439	27.36471366581697	19.385581997712524
21	24.875086212674507	26.89684099464022	27.54762507053764	20.68044772214763
22	22.293138227834962	26.563016870658284	26.68474159980455	24.4591033017022
23	23.06326875220261	27.100292096108486	27.468709393560047	22.367729758128853
24	23.11472609773869	24.906003861462988	28.094629626026173	23.88464041477215
25	21.780726845816066	28.180031523029324	26.267461444827134	23.771780186327483
26	22.033905634146922	28.03949675580895	26.61814974087551	23.30844786916862
27	24.635960901065687	27.732482341265978	26.366268196549765	21.26528856111857
28	20.637854877313146	28.521422903707737	28.022632583742507	22.818089635236607
29	23.790806431735778	26.86657196785429	26.20022096389554	23.14240063651439
30	23.747348757564556	26.288433556243096	26.210815123270613	23.75340256292174
31	23.274719525035728	28.835572160278822	24.33910823122942	23.55060008345603
32	24.949461535634214	27.51411293373894	24.746875263502687	22.789550267124163
33	22.945868169740056	28.10954793208495	24.787089827661134	24.15749407051386
34	21.499224896706945	26.876949919895182	28.263055139356435	23.360770044041434
35	23.13267130647606	24.729578676767876	26.965378719576925	25.17237129717914
36	22.880573554816127	29.522679068319356	25.103184950239882	22.493562426624635
37	22.555830138869972	28.44423688540363	27.43173793941438	21.56819503631202
38	20.80865867131945	27.469574222896785	26.031363035896902	25.690404069886856
39	23.16985896795591	25.06188934941051	26.735334116003884	25.0329175666297
40	22.922950192316424	24.412186310184016	29.90277156181692	22.762091935682644
41	20.12220038528147	27.06396926396537	28.26932515204781	24.544505198705348
42	22.192385610104665	27.160830149680336	28.14435731288877	22.50242692732623
43	23.80637335979711	26.05665929399657	26.327999498398984	23.808967847807335
44	22.376594258830448	26.259245566128094	26.23373310069424	25.130427074347217
45	22.28211165379152	23.703674876059146	27.128182842218372	26.886030627930964
46	22.244707784977482	26.719118565939993	26.499884329076213	24.536289320006315
47	21.723431902256987	25.45668394163267	29.989686910159367	22.830197245950977
48	21.528196679487763	24.924165277534545	28.45612828878381	25.09150975419388
49	22.18503456074237	24.173493413243563	29.696077350335877	23.945394675678187
50	20.592451337134257	24.29240744704542	29.11880376806142	25.996337447758904
51	20.371271234262807	23.83793963058815	27.41746825535816	28.373320879790885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	211.0
1	439.0
2	667.0
3	913.0
4	1159.0
5	866.5
6	574.0
7	594.5
8	615.0
9	588.0
10	561.0
11	590.0
12	619.0
13	694.5
14	770.0
15	810.5
16	851.0
17	861.5
18	872.0
19	931.0
20	990.0
21	1054.0
22	1118.0
23	1283.0
24	1448.0
25	2221.0
26	3580.5
27	4167.0
28	5234.0
29	6301.0
30	6814.5
31	7328.0
32	7851.5
33	8375.0
34	9710.5
35	11046.0
36	12305.5
37	13565.0
38	14799.5
39	16034.0
40	18183.0
41	20332.0
42	22696.5
43	25061.0
44	27885.5
45	30710.0
46	35898.5
47	41087.0
48	44140.0
49	47193.0
50	46333.5
51	45474.0
52	40092.0
53	34710.0
54	31050.5
55	27391.0
56	25731.0
57	24071.0
58	22531.5
59	20992.0
60	20827.0
61	20662.0
62	19014.5
63	17367.0
64	13635.5
65	9904.0
66	8285.5
67	6667.0
68	5343.0
69	4019.0
70	3589.0
71	3159.0
72	2326.0
73	1493.0
74	1221.5
75	792.0
76	634.0
77	386.0
78	138.0
79	151.0
80	164.0
81	110.0
82	56.0
83	33.0
84	10.0
85	6.5
86	3.0
87	2.5
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	462519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.22344168745217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2563792834078	22.24869403414458
2	10.9026359364911	6.8083563480510065
3	3.962895509647884	3.712057105768702
4	1.9811079574739945	2.47428035146947
5	1.2695977115401254	1.9820605056397913
6	0.871053786445431	1.6318378264627997
7	0.7191069480089034	1.5717095700738388
8	0.6449441143413324	1.6109899956641658
9	0.5866184069110765	1.6484621058876678
>10	7.607598690989445	48.709179242077006
>50	0.13717344286080918	2.8567353007953447
>100	0.056689024855742565	3.391676631503446
>500	0.002799458017567534	0.7130365858253813
>1k	0.001399729008783767	0.6409243966367894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1594	0.34463449069119323	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1339	0.2895016204739697	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	918	0.19847833278200464	TruSeq Adapter, Index 20 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	838	0.18118174604718942	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	793	0.17145241600885586	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	714	0.15437203660822582	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	493	0.10659021575329879	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	488	0.10550917908237283	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	479	0.10356331307470612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.207991455486153	0.0
2	0.0	0.0	0.0	1.0533621321502469	0.0
3	0.0	0.0	0.0	1.4600481277525896	0.0
4	0.0	0.0	0.0	2.007917512577862	0.0
5	0.0	0.0	0.0	3.681362279171234	0.0
6	0.0	0.0	0.0	4.462735584916511	0.0
7	0.0	0.0	0.0	5.236325426631122	0.0
8	0.0	0.0	0.0	6.436492338693113	0.0
9	0.0	0.0	0.0	6.863934238377234	0.0
10	0.0	0.0	0.0	8.05804734508204	0.0
11	0.0	0.0	0.0	9.507933728127925	0.0
12	0.0	0.0	0.0	10.81577189261414	0.0
13	0.0	0.0	0.0	11.300508735857338	0.0
14	0.0	0.0	0.0	11.516283655374158	0.0
15	0.0	0.0	0.0	11.955616958438464	0.0
16	0.0	0.0	0.0	12.799041769094892	0.0
17	0.0	0.0	0.0	13.701491181983876	0.0
18	0.0	0.0	0.0	14.673775563814676	0.0
19	0.0	0.0	0.0	15.284345075553652	0.0
20	2.1620733418519023E-4	0.0	0.0	15.91653532071115	0.0
21	2.1620733418519023E-4	0.0	0.0	16.630884352859017	0.0
22	2.1620733418519023E-4	0.0	0.0	17.39625831587459	0.0
23	2.1620733418519023E-4	0.0	0.0	18.11687736071383	0.0
24	2.1620733418519023E-4	0.0	0.0	18.695664394327583	0.0
25	2.1620733418519023E-4	0.0	0.0	19.23077754643593	0.0
26	2.1620733418519023E-4	0.0	0.0	19.706866096311717	0.0
27	2.1620733418519023E-4	0.0	0.0	20.204142964937656	0.0
28	2.1620733418519023E-4	0.0	0.0	20.708122260923336	0.0
29	2.1620733418519023E-4	0.0	0.0	21.183778396130755	0.0
30	2.1620733418519023E-4	0.0	0.0	21.751322648366877	0.0
31	4.3241466837038047E-4	0.0	0.0	22.280598202452225	0.0
32	4.3241466837038047E-4	0.0	0.0	22.807063061193162	0.0
33	6.486220025555707E-4	0.0	0.0	23.324231004564137	0.0
34	6.486220025555707E-4	0.0	0.0	23.823237531863555	0.0
35	6.486220025555707E-4	0.0	0.0	24.327649242517605	0.0
36	8.648293367407609E-4	0.0	0.0	24.854546515926913	0.0
37	8.648293367407609E-4	0.0	0.0	25.36803893461674	0.0
38	8.648293367407609E-4	0.0	0.0	25.866180632579418	0.0
39	8.648293367407609E-4	0.0	0.0	26.37361924591206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	30	2.1626784E-6	45.000004	2
CATATCG	20	7.0289115E-4	45.0	16
GGTCGTT	20	7.0289115E-4	45.0	8
TTCACGG	25	3.887178E-5	45.0	2
TTCGTTA	20	7.0289115E-4	45.0	42
GCGTTAA	20	7.0289115E-4	45.0	34
CGTTAAT	20	7.0289115E-4	45.0	44
CGTTAAC	20	7.0289115E-4	45.0	35
TCGGACG	20	7.0289115E-4	45.0	2
CTACGGT	25	3.887178E-5	45.0	28
TACGACC	20	7.0289115E-4	45.0	35
ACGTGCG	20	7.0289115E-4	45.0	1
ACTACGG	20	7.0289115E-4	45.0	27
TGTAGCG	20	7.0289115E-4	45.0	1
TTACGCG	20	7.0289115E-4	45.0	1
TTACGAC	20	7.0289115E-4	45.0	34
ACGTAAG	20	7.0289115E-4	45.0	1
TATACGG	20	7.0289115E-4	45.0	2
GCATCGT	20	7.0289115E-4	45.0	25
GGCTAAT	25	3.887178E-5	45.0	8
>>END_MODULE