SRR2935939.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935939.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843773
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1540	0.18251354333452244	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.16805467821321612	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1216	0.14411459006154498	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1128	0.1336852447281437	RNA PCR Primer, Index 38 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	858	0.1016861170006625	RNA PCR Primer, Index 38 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	854	0.10121205584914426	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.0322177E-4	45.000004	21
TCTAGCG	20	7.0322177E-4	45.000004	1
ACGACCC	20	7.0322177E-4	45.000004	15
TATAGCG	45	3.8380676E-10	45.0	1
TACGGGT	75	0.0	42.0	4
CGGTCTA	50	1.0804797E-9	40.500004	31
CGGGTAC	85	0.0	39.705883	6
CGAAAGG	120	0.0	39.375004	2
TTGAGCG	65	9.094947E-12	38.07692	1
TCGTCCC	190	0.0	37.894737	38
ACAACGA	125	0.0	37.800003	13
ACGGGAG	435	0.0	37.75862	5
TGATCGG	30	1.1396644E-4	37.500004	2
TCGTTAG	30	1.1396644E-4	37.500004	1
TAACGCC	30	1.1396644E-4	37.500004	12
CTAGGCG	30	1.1396644E-4	37.500004	1
CGTTTTT	860	0.0	37.15116	1
CTCGTCC	205	0.0	36.219513	37
CCTCGTC	205	0.0	36.219513	36
TCACGAC	50	4.8763468E-8	36.000004	25