##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935939.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843773
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17915244977026	33.0	31.0	34.0	30.0	34.0
2	32.2951255847248	34.0	31.0	34.0	30.0	34.0
3	32.351237832924255	34.0	31.0	34.0	30.0	34.0
4	35.88367605979333	37.0	35.0	37.0	35.0	37.0
5	35.90089159051072	37.0	35.0	37.0	35.0	37.0
6	35.90887122484365	37.0	35.0	37.0	35.0	37.0
7	36.0672100197565	37.0	35.0	37.0	35.0	37.0
8	35.79071740859212	37.0	35.0	37.0	35.0	37.0
9	37.52238220469249	39.0	37.0	39.0	35.0	39.0
10	37.20387236851618	39.0	37.0	39.0	34.0	39.0
11	37.17132925561733	39.0	37.0	39.0	34.0	39.0
12	37.08273433731584	39.0	37.0	39.0	34.0	39.0
13	37.04023831054087	39.0	37.0	39.0	33.0	39.0
14	38.12471363743566	40.0	37.0	41.0	33.0	41.0
15	38.31131595820203	40.0	38.0	41.0	33.0	41.0
16	38.44120041764788	40.0	38.0	41.0	34.0	41.0
17	38.4302555308122	40.0	38.0	41.0	34.0	41.0
18	38.42464501708398	40.0	37.0	41.0	34.0	41.0
19	38.43291145841358	40.0	37.0	41.0	34.0	41.0
20	38.282745477752904	40.0	37.0	41.0	34.0	41.0
21	38.268512976831445	40.0	37.0	41.0	34.0	41.0
22	38.27183614550359	40.0	37.0	41.0	34.0	41.0
23	38.28772430499672	40.0	37.0	41.0	34.0	41.0
24	38.20449575893042	40.0	37.0	41.0	34.0	41.0
25	38.12569968463082	40.0	37.0	41.0	34.0	41.0
26	37.98897570792144	40.0	36.0	41.0	34.0	41.0
27	37.950173802669674	40.0	36.0	41.0	34.0	41.0
28	37.930462339989546	40.0	36.0	41.0	34.0	41.0
29	37.91705589062461	40.0	36.0	41.0	34.0	41.0
30	37.808747139337235	40.0	36.0	41.0	33.0	41.0
31	37.829063029985555	40.0	36.0	41.0	33.0	41.0
32	37.79645828913701	40.0	36.0	41.0	33.0	41.0
33	37.76996301137865	40.0	36.0	41.0	33.0	41.0
34	37.685395242559316	40.0	36.0	41.0	33.0	41.0
35	37.675916389834704	40.0	36.0	41.0	33.0	41.0
36	37.60556334464364	40.0	36.0	41.0	33.0	41.0
37	37.576117036217084	40.0	36.0	41.0	33.0	41.0
38	37.512974461140615	40.0	35.0	41.0	33.0	41.0
39	37.46059662966224	40.0	35.0	41.0	33.0	41.0
40	37.360964382600535	40.0	35.0	41.0	33.0	41.0
41	37.254020927429536	40.0	35.0	41.0	33.0	41.0
42	37.255146822664386	40.0	35.0	41.0	33.0	41.0
43	37.203802444496326	39.0	35.0	41.0	33.0	41.0
44	37.13161004203737	39.0	35.0	41.0	32.0	41.0
45	37.02789375815534	39.0	35.0	41.0	32.0	41.0
46	36.9913033481754	39.0	35.0	41.0	32.0	41.0
47	36.95401606830273	39.0	35.0	41.0	32.0	41.0
48	36.887897574347605	39.0	35.0	41.0	32.0	41.0
49	36.8266571696416	39.0	35.0	41.0	32.0	41.0
50	36.71690608730073	39.0	35.0	41.0	31.0	41.0
51	35.76838083228546	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	16.0
10	29.0
11	21.0
12	18.0
13	21.0
14	26.0
15	42.0
16	81.0
17	111.0
18	206.0
19	325.0
20	521.0
21	794.0
22	1233.0
23	1829.0
24	2531.0
25	3675.0
26	4946.0
27	5995.0
28	7200.0
29	8606.0
30	10947.0
31	13940.0
32	18537.0
33	27455.0
34	52602.0
35	60713.0
36	56438.0
37	86472.0
38	160984.0
39	317112.0
40	331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.520677954852786	21.821982926687628	24.993452030344653	10.663887088114931
2	32.75703299347099	24.38001690028005	24.974608099571803	17.888342006677153
3	33.02760339570003	24.04675191076273	25.40043352892306	17.52521116461418
4	29.41004274846434	25.3735305585744	25.577021307863607	19.63940538509765
5	26.04290490451816	29.440975238600902	24.158274796657395	20.357845060223543
6	26.734441609295388	34.35651531869354	23.59354944991129	15.315493622099781
7	80.59335864029781	6.4114400437084385	7.996582019097554	4.998619296896203
8	81.36027106816644	5.828463342628883	7.634399299337619	5.176866289867061
9	75.24156378552051	7.8434602671571625	10.118835279156835	6.79614066816549
10	41.76869845325698	25.67657414968244	17.522366797705068	15.032360599355513
11	30.972666819156338	26.47394500653612	23.423598527092	19.129789647215542
12	29.91017726331608	22.040880663401175	27.848485315363252	20.20045675791949
13	26.09647381463972	22.759675884390706	29.737026427724047	21.40682387324553
14	20.261373615889582	26.550979943657833	30.12670469427204	23.06094174618055
15	20.289935800268555	26.30861617994413	32.70204189989488	20.699406119892437
16	24.312818732052342	25.900686559062684	29.15867182287179	20.62782288601318
17	23.574231457986922	26.31928255585329	27.381772111693547	22.724713874466236
18	24.07389191168715	26.67364326661318	28.074968030501097	21.17749679119858
19	25.060294652708727	27.77547989802945	26.035912502533265	21.128312946728563
20	25.361797545074328	27.912246540242457	27.015559872145705	19.710396042537507
21	24.58552240946321	27.007974893721414	28.49628987891293	19.910212817902444
22	23.379510840000805	24.716718833145883	29.17206405040218	22.73170627645113
23	23.33269730128838	25.305858329195175	29.45543410372221	21.906010265794237
24	23.236462887530177	25.48552750562059	28.530066735958602	22.74794287089063
25	23.315038523394325	26.834349996977856	26.25457320867105	23.59603827095676
26	21.28096063751744	27.830233961029805	26.47050806318761	24.41829733826515
27	22.39251552253983	27.310188877814294	28.031354404561416	22.26594119508446
28	20.099126186782463	28.32681301724516	27.347047132344837	24.227013663627538
29	22.37912329500944	26.28029102614092	27.397416129693653	23.943169549155993
30	24.015226844186767	25.55557004075741	27.611691770179895	22.817511344875932
31	23.07314882083214	28.07567912222837	25.701699390712907	23.149472666226583
32	24.494621183659586	27.274515776162545	25.22906042264922	23.00180261752865
33	24.33498109088582	26.96898336400904	26.502033129763575	22.19400241534157
34	21.37731356656352	26.921932794720853	28.360708389578715	23.340045249136914
35	21.856826421324218	26.475367189990674	27.420052549678648	24.247753839006464
36	23.822639501382483	28.299198955169224	26.13748010424605	21.740681439202252
37	22.832088725285118	28.938470417991567	26.919325458387505	21.310115398335807
38	22.134626256113908	27.208621276101514	27.242398133147184	23.414354334637398
39	23.026927858559116	25.493586545196393	27.46508835907288	24.014397237171607
40	23.320253196061024	24.64952066491817	29.93980608528597	22.09042005373483
41	21.243272775971736	26.06720053853347	28.577354335822548	24.112172349672246
42	22.296636654645265	25.417855276241358	28.650952329595757	23.63455573951762
43	22.646493784465726	25.14325535422442	28.19182410435034	24.018426756959514
44	22.581784437283485	25.290925402922348	27.109898041297836	25.017392118496325
45	21.97557873977954	24.38890554687102	27.07718782184308	26.558327891506366
46	22.127041277689617	26.167227441503815	27.38414241745114	24.32158886335543
47	20.74894551022609	25.93778184416899	29.970620060134657	23.342652585470265
48	20.98289468850034	25.144084961239578	29.323763618888016	24.54925673137206
49	22.246267657296453	24.929453774889694	29.134613219432243	23.689665348381613
50	20.51262602619425	24.158393311945275	29.774832804557622	25.55414785730285
51	19.940671246887494	24.540723630644734	28.484438350124975	27.0341667723428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	269.0
1	632.5
2	996.0
3	1869.5
4	2743.0
5	1911.0
6	1079.0
7	1118.5
8	1158.0
9	1138.5
10	1119.0
11	1082.5
12	1046.0
13	1088.5
14	1131.0
15	1141.0
16	1151.0
17	1294.0
18	1437.0
19	1592.5
20	1748.0
21	2126.5
22	2505.0
23	2611.0
24	2717.0
25	3603.0
26	5989.5
27	7490.0
28	9217.5
29	10945.0
30	12766.5
31	14588.0
32	16767.0
33	18946.0
34	22468.0
35	25990.0
36	26746.0
37	27502.0
38	29272.5
39	31043.0
40	33639.5
41	36236.0
42	40452.0
43	44668.0
44	48730.0
45	52792.0
46	59348.0
47	65904.0
48	72662.5
49	79421.0
50	79019.0
51	78617.0
52	70731.5
53	62846.0
54	56541.5
55	50237.0
56	48684.5
57	47132.0
58	45071.0
59	43010.0
60	40443.5
61	37877.0
62	33401.0
63	28925.0
64	25062.0
65	21199.0
66	17570.5
67	13942.0
68	11571.0
69	9200.0
70	7281.5
71	5363.0
72	4288.5
73	3214.0
74	2426.5
75	1186.5
76	734.0
77	541.0
78	348.0
79	271.5
80	195.0
81	173.0
82	151.0
83	84.0
84	17.0
85	11.0
86	5.0
87	3.5
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	843773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.336873645163365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.59460098628165	20.710248891652697
2	10.811949430702104	6.343775886128071
3	3.8547045090167313	3.39254937361396
4	1.9163911531864501	2.2488370046295922
5	1.2426439527708544	1.8227644314182456
6	0.9315916728362504	1.639799231693007
7	0.7524827027682364	1.545284297989794
8	0.6451910071437202	1.514230964285681
9	0.5669417069231614	1.4969067498160222
>10	8.51151082273059	53.32375619827806
>50	0.12051699905351189	2.339842777081955
>100	0.04698120244035567	2.452861845153643
>500	0.0028597253659346927	0.5336946055149601
>1k	0.0016341287805341099	0.6354477427442888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1540	0.18251354333452244	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.16805467821321612	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1216	0.14411459006154498	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1128	0.1336852447281437	RNA PCR Primer, Index 38 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	858	0.1016861170006625	RNA PCR Primer, Index 38 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	854	0.10121205584914426	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.740611515182401E-4	0.0	0.0	0.10547860621280843	0.0
2	4.740611515182401E-4	0.0	0.0	0.5464739924126513	0.0
3	4.740611515182401E-4	0.0	0.0	0.8208368838538327	0.0
4	4.740611515182401E-4	0.0	0.0	1.233151570386822	0.0
5	4.740611515182401E-4	0.0	0.0	2.4234006065612435	0.0
6	4.740611515182401E-4	0.0	0.0	3.152980718747815	0.0
7	4.740611515182401E-4	0.0	0.0	3.807422138418745	0.0
8	4.740611515182401E-4	0.0	0.0	4.7857658398645135	0.0
9	4.740611515182401E-4	0.0	0.0	5.158496420245729	0.0
10	4.740611515182401E-4	0.0	0.0	6.002680815811836	0.0
11	4.740611515182401E-4	0.0	0.0	7.261905749532161	0.0
12	5.925764393978001E-4	0.0	0.0	8.225909101144502	0.0
13	5.925764393978001E-4	0.0	0.0	8.628268503495608	0.0
14	7.110917272773601E-4	0.0	0.0	8.797271304011861	0.0
15	7.110917272773601E-4	0.0	0.0	9.117499611862431	0.0
16	7.110917272773601E-4	0.0	0.0	9.735556838154338	0.0
17	7.110917272773601E-4	0.0	0.0	10.444041229098348	0.0
18	7.110917272773601E-4	0.0	0.0	11.20301313267905	0.0
19	8.296070151569202E-4	0.0	0.0	11.69319236334891	0.0
20	9.481223030364802E-4	0.0	0.0	12.19154914888246	0.0
21	0.0010666375909160401	0.0	0.0	12.817309868886538	0.0
22	0.0011851528787956002	0.0	0.0	13.476017838921132	0.0
23	0.0013036681666751602	0.0	0.0	14.091349213591807	0.0
24	0.0013036681666751602	0.0	0.0	14.603809318383025	0.0
25	0.0013036681666751602	0.0	0.0	15.050967499552605	0.0
26	0.0013036681666751602	0.0	0.0	15.455815722949183	0.0
27	0.0014221834545547203	0.0	0.0	15.95215774858878	0.0
28	0.0014221834545547203	0.0	0.0	16.397893746303804	0.0
29	0.0014221834545547203	0.0	0.0	16.88096205970089	0.0
30	0.0014221834545547203	0.0	0.0	17.466072035962277	0.0
31	0.0014221834545547203	0.0	0.0	17.954947598465463	0.0
32	0.0014221834545547203	0.0	0.0	18.429482811135223	0.0
33	0.0014221834545547203	0.0	0.0	18.88007793565331	0.0
34	0.0014221834545547203	0.0	0.0	19.33505812582294	0.0
35	0.0014221834545547203	0.0	0.0	19.847873776477797	0.0
36	0.0015406987424342804	0.0	0.0	20.328216238253653	0.0
37	0.0016592140303138404	0.0	0.0	20.82467677918113	0.0
38	0.0016592140303138404	0.0	0.0	21.362617670866452	0.0
39	0.0017777293181934003	0.0	0.0	22.085797957507527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.0322177E-4	45.000004	21
TCTAGCG	20	7.0322177E-4	45.000004	1
ACGACCC	20	7.0322177E-4	45.000004	15
TATAGCG	45	3.8380676E-10	45.0	1
TACGGGT	75	0.0	42.0	4
CGGTCTA	50	1.0804797E-9	40.500004	31
CGGGTAC	85	0.0	39.705883	6
CGAAAGG	120	0.0	39.375004	2
TTGAGCG	65	9.094947E-12	38.07692	1
TCGTCCC	190	0.0	37.894737	38
ACAACGA	125	0.0	37.800003	13
ACGGGAG	435	0.0	37.75862	5
TGATCGG	30	1.1396644E-4	37.500004	2
TCGTTAG	30	1.1396644E-4	37.500004	1
TAACGCC	30	1.1396644E-4	37.500004	12
CTAGGCG	30	1.1396644E-4	37.500004	1
CGTTTTT	860	0.0	37.15116	1
CTCGTCC	205	0.0	36.219513	37
CCTCGTC	205	0.0	36.219513	36
TCACGAC	50	4.8763468E-8	36.000004	25
>>END_MODULE