##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935936.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445013
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10494974304122	33.0	31.0	34.0	30.0	34.0
2	32.223905818481704	33.0	31.0	34.0	30.0	34.0
3	32.28619838072146	34.0	31.0	34.0	30.0	34.0
4	35.845413504774015	37.0	35.0	37.0	35.0	37.0
5	35.879513632186026	37.0	35.0	37.0	35.0	37.0
6	35.91071496787734	37.0	35.0	37.0	35.0	37.0
7	36.051717590272645	37.0	35.0	37.0	35.0	37.0
8	35.72310921253986	37.0	35.0	37.0	35.0	37.0
9	37.48493190086582	39.0	37.0	39.0	35.0	39.0
10	37.26461698871719	39.0	37.0	39.0	34.0	39.0
11	37.258601434115405	39.0	37.0	39.0	34.0	39.0
12	37.10839458622557	39.0	37.0	39.0	34.0	39.0
13	37.064499239348066	39.0	37.0	39.0	33.0	39.0
14	38.22688550671553	40.0	37.0	41.0	34.0	41.0
15	38.353466078519055	40.0	37.0	41.0	34.0	41.0
16	38.44963630275969	40.0	38.0	41.0	34.0	41.0
17	38.380806852833516	40.0	37.0	41.0	34.0	41.0
18	38.38949873374486	40.0	37.0	41.0	34.0	41.0
19	38.39470307608991	40.0	37.0	41.0	34.0	41.0
20	38.26028003676297	40.0	37.0	41.0	34.0	41.0
21	38.24984663369385	40.0	37.0	41.0	34.0	41.0
22	38.24432994092307	40.0	37.0	41.0	34.0	41.0
23	38.263787799457546	40.0	37.0	41.0	34.0	41.0
24	38.18608669859083	40.0	37.0	41.0	34.0	41.0
25	38.10806425879694	40.0	36.0	41.0	34.0	41.0
26	37.93288061247649	40.0	36.0	41.0	34.0	41.0
27	37.90182084568316	40.0	36.0	41.0	34.0	41.0
28	37.894832285798394	40.0	36.0	41.0	34.0	41.0
29	37.902836546348084	40.0	36.0	41.0	34.0	41.0
30	37.81242345729226	40.0	36.0	41.0	33.0	41.0
31	37.84045409909373	40.0	36.0	41.0	34.0	41.0
32	37.73879190046134	40.0	36.0	41.0	33.0	41.0
33	37.70179298132864	40.0	36.0	41.0	33.0	41.0
34	37.61678872302607	40.0	36.0	41.0	33.0	41.0
35	37.606609245123174	40.0	36.0	41.0	33.0	41.0
36	37.56595874727255	40.0	36.0	41.0	33.0	41.0
37	37.49160361607414	40.0	35.0	41.0	33.0	41.0
38	37.43906807216868	40.0	35.0	41.0	33.0	41.0
39	37.361108551885	40.0	35.0	41.0	33.0	41.0
40	37.244199607651915	39.0	35.0	41.0	32.0	41.0
41	37.19142811558314	39.0	35.0	41.0	32.0	41.0
42	37.180987971137924	39.0	35.0	41.0	32.0	41.0
43	37.090269273032476	39.0	35.0	41.0	32.0	41.0
44	37.021484765613586	39.0	35.0	41.0	32.0	41.0
45	36.93099302716999	39.0	35.0	41.0	32.0	41.0
46	36.881284366973546	39.0	35.0	41.0	32.0	41.0
47	36.82966115596623	39.0	35.0	41.0	32.0	41.0
48	36.79428466134697	39.0	35.0	41.0	32.0	41.0
49	36.74307716853215	39.0	35.0	41.0	32.0	41.0
50	36.62140431852553	39.0	35.0	41.0	31.0	41.0
51	35.714280256981255	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	8.0
10	12.0
11	5.0
12	13.0
13	9.0
14	8.0
15	15.0
16	25.0
17	39.0
18	66.0
19	136.0
20	240.0
21	421.0
22	680.0
23	971.0
24	1415.0
25	1953.0
26	2583.0
27	3114.0
28	3714.0
29	4470.0
30	5755.0
31	7363.0
32	9751.0
33	13790.0
34	27897.0
35	36954.0
36	30611.0
37	46129.0
38	85852.0
39	160862.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40432077265159	22.12317392975037	22.795064413848586	10.677440883749464
2	31.587391829002748	25.17656787554521	24.33794068937312	18.898099606078926
3	33.322397323224266	25.188702352515545	24.750512906364534	16.738387417895655
4	29.014657998755094	25.468244747906244	25.44307694381962	20.074020309519046
5	26.641693613445	29.864520811751564	23.543806585425596	19.94997898937784
6	26.788880324844445	35.3018900571444	22.8568603613827	15.05236925662846
7	78.77859747917476	6.743173794922845	9.315458200097524	5.162770525804864
8	78.29411725050728	6.884742692910095	8.949401478159064	5.871738578423551
9	72.17497016941078	8.273690880940556	11.25562624013231	8.295712709516351
10	40.0804021455553	28.556469136856677	17.51813991950797	13.844988798080058
11	33.07948307128106	25.270722428333553	22.32519050005281	19.324604000332574
12	31.406273524593665	21.346567403648883	27.018311824598385	20.22884724715907
13	27.417401289400534	24.255695901018623	27.57896960313519	20.747933206445655
14	19.805938253489224	29.615314608786708	27.212238743587264	23.3665083941368
15	19.60414639572327	26.569785601768935	33.79204652448355	20.03402147802424
16	22.92225170950062	25.632284899542263	31.14695525748686	20.298508133470257
17	21.375555320855796	26.194290953297994	27.29493295701474	25.135220768831473
18	23.2678595906187	27.05359169282695	27.99378894549148	21.68475977106287
19	25.03477426502147	28.645005876232833	25.172073624815454	21.148146233930245
20	28.684105857581688	26.236312197621192	26.603492482242093	18.476089462555027
21	25.66846361791678	27.345942702797448	26.147775458245043	20.83781822104073
22	23.09820162557049	25.527119432466016	26.758094707345627	24.616584234617864
23	23.915481120776246	26.75719585719968	27.627507511016532	21.699815511007543
24	24.60062964452724	24.156148247354572	27.109769826948877	24.133452281169316
25	22.60900243363677	27.230665171579254	25.413414889003246	24.74691750578073
26	21.433980580342595	27.86075912389076	28.03288892684034	22.6723713689263
27	23.285611881001227	27.484815050346846	27.192688752912836	22.03688431573909
28	19.347974104127292	28.71511618761699	27.908398181626154	24.028511526629558
29	22.174857813142538	26.620795347551645	28.1870417268709	23.01730511243492
30	22.118454966484123	25.292968969445834	28.79061959987686	23.79795646419318
31	22.548105336248604	26.67405221869923	24.75163646904697	26.026205976005194
32	23.63301746241121	28.029967663866	25.439930968308794	22.897083905413997
33	23.01011431126731	26.544842510218803	25.158366160089706	25.286677018424182
34	20.858941199470575	25.942837624968256	27.88098325217466	25.31723792338651
35	19.808185378854102	25.693181996930427	27.598070168736644	26.900562455478827
36	21.015116412329526	28.35018302836097	25.884637078017946	24.750063481291555
37	21.691725859693985	27.55065582353774	26.34979202854748	24.407826288220793
38	20.993993433899682	27.332235238071696	23.799978877021573	27.873792451007052
39	23.968063854314366	24.273897616474123	24.95657430232375	26.801464226887756
40	22.72135870188062	21.804082127937836	28.8811787520814	26.593380418100143
41	19.248426450463242	25.034100127412007	27.34167316460418	28.375800257520567
42	22.54181338522695	24.382209059061196	27.59537361829879	25.480603937413065
43	23.156402172520803	24.730288778080638	26.87134982573543	25.24195922366313
44	21.697793098179154	24.90915995712485	26.1722691247222	27.220777819973797
45	22.031715927399873	23.253028563210513	26.121484091475978	28.593771417913633
46	21.740039055038842	24.831184706963615	26.246648974299625	27.182127263697915
47	20.076941572493386	24.258167738919987	29.63621287468007	26.028677813906558
48	20.961634828645455	22.458894459262986	28.77556385993218	27.803906852159376
49	21.58296499203394	22.313954873228422	30.193724677706047	25.90935545703159
50	19.35920973095168	23.175727450658744	28.836460957320348	28.628601861069225
51	18.96281681658738	22.032390065009334	27.054041117899924	31.950752000503357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	485.0
2	717.0
3	1037.0
4	1357.0
5	991.5
6	626.0
7	617.0
8	608.0
9	632.0
10	656.0
11	645.5
12	635.0
13	655.0
14	675.0
15	697.5
16	720.0
17	797.0
18	874.0
19	835.5
20	797.0
21	862.5
22	928.0
23	1201.0
24	1474.0
25	1763.5
26	2606.0
27	3159.0
28	3837.0
29	4515.0
30	5513.5
31	6512.0
32	7235.0
33	7958.0
34	8760.5
35	9563.0
36	10219.5
37	10876.0
38	12166.0
39	13456.0
40	15432.0
41	17408.0
42	19105.5
43	20803.0
44	23075.5
45	25348.0
46	27640.0
47	29932.0
48	34807.5
49	39683.0
50	43998.0
51	48313.0
52	48316.5
53	48320.0
54	41157.0
55	33994.0
56	29399.0
57	24804.0
58	23676.5
59	22549.0
60	21988.0
61	21427.0
62	19378.0
63	17329.0
64	14266.0
65	11203.0
66	8786.0
67	6369.0
68	5022.5
69	3676.0
70	2921.0
71	2166.0
72	1815.0
73	1464.0
74	1285.5
75	738.0
76	369.0
77	256.5
78	144.0
79	127.5
80	111.0
81	83.0
82	55.0
83	35.0
84	15.0
85	9.5
86	4.0
87	4.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.573827705951285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.59761866610275	21.974632205080276
2	12.16071574721232	7.67920687567061
3	4.444200973088281	4.209613074447313
4	2.286122188279233	2.887265123499233
5	1.3488811811238817	2.129467100430275
6	0.9404555947174196	1.7816269747623454
7	0.7474507039633662	1.6519915821941926
8	0.6477486891017856	1.6361524405164467
9	0.523246212014086	1.486879717133197
>10	7.126775008823472	47.1194298242014
>50	0.1135448602463299	2.4221749680253977
>100	0.05820970683514381	3.280090055060904
>500	0.0014372767119788593	0.33399920870570354
>1k	0.0035931917799471485	1.407470850272744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1735	0.38987625080615623	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1186	0.26650906827440995	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1125	0.2528016035486604	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGC	1106	0.2485320653553941	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG	1051	0.2361728758485707	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	935	0.21010622161599773	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.12067063209389389	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	491	0.11033385541545979	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.494250729753962E-4	0.0	0.0	0.2615653924716806	0.0
2	4.494250729753962E-4	0.0	0.0	1.1534494497913543	0.0
3	4.494250729753962E-4	0.0	0.0	1.5990544096464598	0.0
4	4.494250729753962E-4	0.0	0.0	2.238136863417473	0.0
5	4.494250729753962E-4	0.0	0.0	4.0338147424906685	0.0
6	4.494250729753962E-4	0.0	0.0	4.866599402714078	0.0
7	4.494250729753962E-4	0.0	0.0	5.755562197059412	0.0
8	4.494250729753962E-4	0.0	0.0	7.199340243992872	0.0
9	6.741376094630944E-4	0.0	0.0	7.688539435926591	0.0
10	6.741376094630944E-4	0.0	0.0	9.115014617550498	0.0
11	6.741376094630944E-4	0.0	0.0	10.833391384071925	0.0
12	6.741376094630944E-4	0.0	0.0	12.484579102183531	0.0
13	6.741376094630944E-4	0.0	0.0	13.067708134369107	0.0
14	6.741376094630944E-4	0.0	0.0	13.29781377173251	0.0
15	8.988501459507924E-4	0.0	0.0	13.809259504778511	0.0
16	8.988501459507924E-4	0.0	0.0	14.709682638484718	0.0
17	8.988501459507924E-4	0.0	0.0	15.751449957641688	0.0
18	8.988501459507924E-4	0.0	0.0	16.89523676836407	0.0
19	8.988501459507924E-4	0.0	0.0	17.626001937022064	0.0
20	8.988501459507924E-4	0.0	0.0	18.295420583218917	0.0
21	8.988501459507924E-4	0.0	0.0	19.1232615676396	0.0
22	8.988501459507924E-4	0.0	0.0	20.052897331089206	0.0
23	8.988501459507924E-4	0.0	0.0	20.911973358081674	0.0
24	8.988501459507924E-4	0.0	0.0	21.534651796689086	0.0
25	8.988501459507924E-4	0.0	0.0	22.111713590389495	0.0
26	8.988501459507924E-4	0.0	0.0	22.650349540350508	0.0
27	8.988501459507924E-4	0.0	0.0	23.195951578942637	0.0
28	0.0011235626824384905	0.0	0.0	23.770316822205196	0.0
29	0.0011235626824384905	0.0	0.0	24.333446438643364	0.0
30	0.0011235626824384905	0.0	0.0	25.012078298836215	0.0
31	0.0011235626824384905	0.0	0.0	25.614083184086756	0.0
32	0.0011235626824384905	0.0	0.0	26.223278870504906	0.0
33	0.0011235626824384905	0.0	0.0	26.809329165664824	0.0
34	0.0011235626824384905	0.0	0.0	27.33875190162984	0.0
35	0.0011235626824384905	0.0	0.0	27.937610811369556	0.0
36	0.0011235626824384905	0.0	0.0	28.56972717650945	0.0
37	0.0011235626824384905	0.0	0.0	29.219371119495385	0.0
38	0.0011235626824384905	0.0	0.0	29.866318512043467	0.0
39	0.0011235626824384905	0.0	0.0	30.495963039282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAGCG	30	2.1624928E-6	45.000004	1
TATCAAC	20	7.0286234E-4	45.0	26
GCGAAAG	25	3.8869395E-5	45.0	1
TAGCGCG	20	7.0286234E-4	45.0	1
GGATCGA	25	3.8869395E-5	45.0	8
TTCGTAC	35	1.2096825E-7	45.0	33
ATCACCC	40	6.7993824E-9	45.0	36
TCTAGCG	35	1.2096825E-7	45.0	1
CGTACAT	35	1.2096825E-7	45.0	35
TCGTAAG	25	3.8869395E-5	45.0	1
CGCACGG	80	0.0	45.0	2
ATTATCG	25	3.8869395E-5	45.0	30
CCACGTT	20	7.0286234E-4	45.0	43
AATCGAT	20	7.0286234E-4	45.0	40
CACAACG	210	0.0	42.857143	12
TAGGGTC	85	0.0	42.352943	5
ACAACGA	220	0.0	41.93182	13
AAACGGG	70	0.0	41.785713	3
CGTAAGG	60	3.6379788E-12	41.250004	2
AGCGACT	200	0.0	40.5	19
>>END_MODULE