##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935935.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641339
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17902076748802	33.0	31.0	34.0	30.0	34.0
2	32.283597286302566	34.0	31.0	34.0	30.0	34.0
3	32.342170053591005	34.0	31.0	34.0	30.0	34.0
4	35.86792944137188	37.0	35.0	37.0	35.0	37.0
5	35.908087610452505	37.0	35.0	37.0	35.0	37.0
6	35.935141945211505	37.0	35.0	37.0	35.0	37.0
7	36.09827407969888	37.0	35.0	37.0	35.0	37.0
8	35.79965197812701	37.0	35.0	37.0	35.0	37.0
9	37.534544133445806	39.0	37.0	39.0	35.0	39.0
10	37.26529807169064	39.0	37.0	39.0	34.0	39.0
11	37.23304991587912	39.0	37.0	39.0	34.0	39.0
12	37.171405450159746	39.0	37.0	39.0	34.0	39.0
13	37.16257236812357	39.0	37.0	39.0	34.0	39.0
14	38.36822179845604	40.0	38.0	41.0	34.0	41.0
15	38.49916190969206	40.0	38.0	41.0	34.0	41.0
16	38.57710352871103	40.0	38.0	41.0	35.0	41.0
17	38.55021915087029	40.0	38.0	41.0	34.0	41.0
18	38.52668713426129	40.0	38.0	41.0	34.0	41.0
19	38.54970304316438	40.0	38.0	41.0	34.0	41.0
20	38.42981169085304	40.0	37.0	41.0	34.0	41.0
21	38.4234811854573	40.0	37.0	41.0	34.0	41.0
22	38.41195374053348	40.0	37.0	41.0	34.0	41.0
23	38.45049342079618	40.0	37.0	41.0	34.0	41.0
24	38.3663070544595	40.0	37.0	41.0	34.0	41.0
25	38.289949621027255	40.0	37.0	41.0	34.0	41.0
26	38.1488760234447	40.0	37.0	41.0	34.0	41.0
27	38.11859562571433	40.0	37.0	41.0	34.0	41.0
28	38.097980942995825	40.0	37.0	41.0	34.0	41.0
29	38.1212962255531	40.0	37.0	41.0	34.0	41.0
30	38.00965012263405	40.0	37.0	41.0	34.0	41.0
31	38.026068896480645	40.0	37.0	41.0	34.0	41.0
32	37.97948822697513	40.0	37.0	41.0	34.0	41.0
33	37.949115834215604	40.0	37.0	41.0	34.0	41.0
34	37.87978744470553	40.0	37.0	41.0	33.0	41.0
35	37.885511406603996	40.0	37.0	41.0	33.0	41.0
36	37.80969814715775	40.0	37.0	41.0	33.0	41.0
37	37.799609878706896	40.0	36.0	41.0	33.0	41.0
38	37.71976598959365	40.0	36.0	41.0	33.0	41.0
39	37.662131883450094	40.0	36.0	41.0	33.0	41.0
40	37.60797175908529	40.0	36.0	41.0	33.0	41.0
41	37.51888158992358	40.0	36.0	41.0	33.0	41.0
42	37.487901094429	40.0	36.0	41.0	33.0	41.0
43	37.408715203659845	40.0	35.0	41.0	33.0	41.0
44	37.36361425080963	40.0	35.0	41.0	33.0	41.0
45	37.260200923380616	40.0	35.0	41.0	32.0	41.0
46	37.195135489967086	39.0	35.0	41.0	32.0	41.0
47	37.150843781525836	39.0	35.0	41.0	32.0	41.0
48	37.07371920310475	39.0	35.0	41.0	32.0	41.0
49	37.011301355445404	39.0	35.0	41.0	32.0	41.0
50	36.858823492723815	39.0	35.0	41.0	32.0	41.0
51	35.897336665944216	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	11.0
10	11.0
11	22.0
12	14.0
13	15.0
14	19.0
15	21.0
16	36.0
17	67.0
18	95.0
19	171.0
20	306.0
21	517.0
22	787.0
23	1151.0
24	1629.0
25	2287.0
26	3316.0
27	4146.0
28	5062.0
29	6236.0
30	7640.0
31	9938.0
32	13309.0
33	18481.0
34	36170.0
35	47773.0
36	40967.0
37	64354.0
38	125573.0
39	250988.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.774353345110775	21.504695644581105	24.64125836725975	10.079692643048373
2	32.16972615106831	24.224318184298788	25.168124813865987	18.43783085076691
3	33.0564646778069	23.98107709027519	25.75658115286923	17.20587707904868
4	29.726400546356917	25.43241561794932	25.288497970652024	19.552685865041735
5	25.9811113935064	29.52588256756567	23.894383469584728	20.598622569343203
6	26.58874635723073	34.77474471379411	23.378431687453904	15.258077241521253
7	80.63738522060876	6.219331741871303	7.7511269391070865	5.392156098412852
8	80.50032821955315	5.900623539189103	8.02867126433914	5.570376976918603
9	73.83458670063726	8.84150192020133	10.303755112350878	7.020156266810533
10	38.290358141326195	31.299203697264627	16.848187931811413	13.562250229597764
11	29.007436004983322	25.080963421840867	25.838752983991302	20.0728475891845
12	28.751876932480325	22.603802357255677	28.471525979240308	20.172794731023686
13	25.02077684344785	24.375408325394215	29.27921115042123	21.324603680736708
14	20.23656755631577	27.13853359923535	29.308212973170196	23.316685871278683
15	20.111672609961346	26.66826748412306	33.42709549863645	19.792964407279147
16	23.259773692228293	26.466034343771387	30.275564093248658	19.998627870751662
17	22.725734751823918	26.087139562696173	27.956353816000583	23.230771869479323
18	22.974121330528785	26.786457708014016	29.351559783515423	20.887861177941776
19	24.180659526397115	28.43581943402787	26.90698678857827	20.476534250996743
20	25.377686371794013	27.39424859551657	27.809629540695326	19.418435491994092
21	24.16506714857509	27.06103948145988	28.608115208961248	20.165778161003775
22	22.20713226546335	25.485117854987767	28.397929955920347	23.909819923628532
23	22.153026714420925	25.93636126915719	29.641422087226882	22.26918992919501
24	23.09932812443965	24.187676096417025	30.097810986077565	22.615184793065758
25	22.031406167409123	26.75636441881751	27.392845281512585	23.819384132260783
26	21.297628867104603	27.700171048384707	27.579486044042227	23.422714040468456
27	21.971999207907206	27.349498471167355	28.748758456915922	21.929743864009517
28	20.033554797073	28.041332275130625	29.39693360297752	22.528179324818858
29	22.736961263855775	26.118792089674887	28.90686516803126	22.23738147843808
30	23.899840801822435	26.97980319300713	27.557812638869617	21.56254336630082
31	23.68809631099933	28.216746525628412	25.593952652185504	22.50120451118675
32	24.423900620420714	27.54908090728928	26.89264180098201	21.134376671307997
33	23.83404096741349	27.649651744241343	26.48739590138757	22.0289113869576
34	21.297005171991724	26.941601867343167	28.58160816666381	23.1797847940013
35	23.151874437699874	25.992805676872916	27.747415953185445	23.107903932241765
36	22.9580611813721	28.762011978064645	27.208855223212687	21.071071617350572
37	22.78763649177736	28.236860693018823	27.888994743809437	21.08650807139438
38	21.87719755074929	28.14923152965904	27.296796234128905	22.676774685462757
39	22.17641528115396	25.77529200625566	28.836075772719266	23.212216939871112
40	23.493191588224015	25.092813628985606	28.763415292068622	22.650579490721757
41	21.49721130322653	26.180693829628325	27.9510524075411	24.37104245960405
42	22.850161926843683	25.92638214735109	28.48493542416725	22.73852050163798
43	23.059879408549925	25.054300455765205	29.04236293130466	22.84345720438021
44	21.256932760989116	26.033813630544845	27.99439921788633	24.71485439057971
45	22.515705422561236	25.018282062996327	27.187493665596506	25.278518848845934
46	22.914402523470425	26.97917949789425	27.116236498949853	22.99018147968547
47	21.414415776991575	25.209912386429018	30.016886545181254	23.358785291398153
48	21.731876589448014	24.625042294324842	30.259192096535532	23.383889019691615
49	22.245177667349093	24.029569385301688	30.30659292511449	23.41866002223473
50	20.680638476687058	23.799737736205035	30.581642469895016	24.93798131721289
51	19.841456702305646	23.933052566583353	28.771211480979638	27.454279250131364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	307.0
1	571.5
2	836.0
3	1223.0
4	1610.0
5	1189.0
6	768.0
7	755.0
8	742.0
9	754.0
10	766.0
11	764.0
12	762.0
13	779.0
14	796.0
15	906.0
16	1016.0
17	1080.0
18	1144.0
19	1133.0
20	1122.0
21	1453.0
22	1784.0
23	1960.0
24	2136.0
25	2843.0
26	4848.5
27	6147.0
28	7001.0
29	7855.0
30	9559.0
31	11263.0
32	13151.0
33	15039.0
34	16854.0
35	18669.0
36	19867.5
37	21066.0
38	23251.5
39	25437.0
40	27796.5
41	30156.0
42	33449.5
43	36743.0
44	41374.5
45	46006.0
46	50643.5
47	55281.0
48	61427.5
49	67574.0
50	65196.5
51	62819.0
52	55820.0
53	48821.0
54	43064.0
55	37307.0
56	33759.5
57	30212.0
58	28272.0
59	26332.0
60	25490.0
61	24648.0
62	22363.5
63	20079.0
64	16565.0
65	13051.0
66	10221.0
67	7391.0
68	6142.5
69	4894.0
70	4002.5
71	3111.0
72	2413.0
73	1715.0
74	1466.0
75	980.5
76	744.0
77	478.0
78	212.0
79	144.0
80	76.0
81	103.0
82	130.0
83	66.5
84	3.0
85	1.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	641339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.870498905474847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.74531500060192	20.833273553874783
2	11.573861437449109	6.914340307988823
3	4.294091610848938	3.847999762856159
4	2.110178365363417	2.521283222117786
5	1.2501377410447878	1.8671119012785569
6	0.9061142930001818	1.623965159837823
7	0.7206967035316881	1.5069299065815833
8	0.6143992276717399	1.4681929166154637
9	0.565528226615529	1.520334924672086
>10	8.03317314467021	51.84030305982209
>50	0.12997179972751668	2.548602686047565
>100	0.05230572428055311	2.4338690088677866
>500	0.003170043895791098	0.6525773798254635
>1k	0.0010566812985970326	0.42121620961406575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1586	0.24729511225732412	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1083	0.16886545181253595	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	971	0.1514019886518674	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	890	0.1387721626160268	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.1010386082867251	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5592377822025482E-4	0.0	0.0	0.1479716655310218	0.0
2	1.5592377822025482E-4	0.0	0.0	0.74656305011858	0.0
3	1.5592377822025482E-4	0.0	0.0	1.0691693472562873	0.0
4	1.5592377822025482E-4	0.0	0.0	1.5438013281587428	0.0
5	1.5592377822025482E-4	0.0	0.0	2.8548084554346453	0.0
6	1.5592377822025482E-4	0.0	0.0	3.5684715883487517	0.0
7	1.5592377822025482E-4	0.0	0.0	4.29616786130268	0.0
8	1.5592377822025482E-4	0.0	0.0	5.452654524362311	0.0
9	1.5592377822025482E-4	0.0	0.0	5.850416082602181	0.0
10	1.5592377822025482E-4	0.0	0.0	6.79266347438718	0.0
11	1.5592377822025482E-4	0.0	0.0	8.233555108920555	0.0
12	1.5592377822025482E-4	0.0	0.0	9.433388582325415	0.0
13	1.5592377822025482E-4	0.0	0.0	9.887750472059238	0.0
14	1.5592377822025482E-4	0.0	0.0	10.074235310810664	0.0
15	1.5592377822025482E-4	0.0	0.0	10.466383613034605	0.0
16	1.5592377822025482E-4	0.0	0.0	11.258944177728159	0.0
17	1.5592377822025482E-4	0.0	0.0	12.162990243849197	0.0
18	1.5592377822025482E-4	0.0	0.0	13.12613142191571	0.0
19	1.5592377822025482E-4	0.0	0.0	13.746084364119444	0.0
20	1.5592377822025482E-4	0.0	0.0	14.362451059424112	0.0
21	1.5592377822025482E-4	0.0	0.0	15.130687514715307	0.0
22	1.5592377822025482E-4	0.0	0.0	15.990607151600011	0.0
23	1.5592377822025482E-4	0.0	0.0	16.77350044204391	0.0
24	1.5592377822025482E-4	0.0	0.0	17.376925463756297	0.0
25	1.5592377822025482E-4	0.0	0.0	17.916265812620157	0.0
26	1.5592377822025482E-4	0.0	0.0	18.41225935113879	0.0
27	1.5592377822025482E-4	0.0	0.0	18.911527289000045	0.0
28	1.5592377822025482E-4	0.0	0.0	19.418591415772315	0.0
29	1.5592377822025482E-4	0.0	0.0	19.958555459749057	0.0
30	1.5592377822025482E-4	0.0	0.0	20.57819655439635	0.0
31	3.1184755644050963E-4	0.0	0.0	21.14295247911011	0.0
32	3.1184755644050963E-4	0.0	0.0	21.713789431174465	0.0
33	4.6777133466076445E-4	0.0	0.0	22.252194237369004	0.0
34	4.6777133466076445E-4	0.0	0.0	22.791066814898205	0.0
35	4.6777133466076445E-4	0.0	0.0	23.352860187825783	0.0
36	4.6777133466076445E-4	0.0	0.0	23.90077634449176	0.0
37	4.6777133466076445E-4	0.0	0.0	24.450251738939937	0.0
38	4.6777133466076445E-4	0.0	0.0	25.00767924607735	0.0
39	4.6777133466076445E-4	0.0	0.0	25.5654186007712	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTA	20	7.030953E-4	45.0	16
CGACAAT	20	7.030953E-4	45.0	20
ACGGACG	25	3.8888702E-5	45.0	1
ATACGCG	20	7.030953E-4	45.0	1
TGTCGAA	20	7.030953E-4	45.0	35
TCAACGG	20	7.030953E-4	45.0	2
TAACGCG	30	2.1639953E-6	44.999996	1
CGAATAT	155	0.0	43.54839	14
GCTACGA	165	0.0	42.272728	10
TACGAAT	160	0.0	42.1875	12
CGCGACC	65	0.0	41.538464	10
CGACCCG	60	3.6379788E-12	41.249996	32
GTTTACG	55	6.002665E-11	40.909092	1
TTGCACG	50	1.0804797E-9	40.5	1
ACAACGA	95	0.0	40.263157	13
TTTCGCA	45	1.925946E-8	40.0	14
GCCGTCA	45	1.925946E-8	40.0	29
GGCGTAG	40	3.455043E-7	39.375	1
TACGCGG	80	0.0	39.375	2
CGTAAGG	110	0.0	38.86364	2
>>END_MODULE