##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935931.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422779
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.044999869908395	33.0	31.0	34.0	30.0	34.0
2	32.161207155511505	33.0	31.0	34.0	30.0	34.0
3	32.233497879506785	34.0	31.0	34.0	30.0	34.0
4	35.78702584565458	37.0	35.0	37.0	35.0	37.0
5	35.81509724939034	37.0	35.0	37.0	35.0	37.0
6	35.82723597908127	37.0	35.0	37.0	35.0	37.0
7	36.00885332525977	37.0	35.0	37.0	35.0	37.0
8	35.767968607712305	37.0	35.0	37.0	35.0	37.0
9	37.50017384969452	39.0	37.0	39.0	35.0	39.0
10	37.218892139865034	39.0	37.0	39.0	34.0	39.0
11	37.12340963009043	39.0	37.0	39.0	34.0	39.0
12	36.830329794053156	39.0	35.0	39.0	33.0	39.0
13	36.68748920830978	39.0	35.0	39.0	33.0	39.0
14	37.36147490769409	40.0	36.0	41.0	31.0	41.0
15	37.67170554828882	40.0	36.0	41.0	32.0	41.0
16	37.939330004565036	40.0	36.0	41.0	33.0	41.0
17	37.999687780140455	40.0	36.0	41.0	33.0	41.0
18	38.00519893372187	40.0	36.0	41.0	33.0	41.0
19	38.02131846662204	40.0	36.0	41.0	34.0	41.0
20	37.8804670998323	40.0	36.0	41.0	33.0	41.0
21	37.84730793156708	40.0	36.0	41.0	33.0	41.0
22	37.902019731349	40.0	35.0	41.0	34.0	41.0
23	37.94744772091329	40.0	35.0	41.0	34.0	41.0
24	37.84656286144771	40.0	35.0	41.0	34.0	41.0
25	37.737718760865604	40.0	35.0	41.0	33.0	41.0
26	37.578221718675714	39.0	35.0	41.0	33.0	41.0
27	37.539660200719524	39.0	35.0	41.0	33.0	41.0
28	37.52068811364803	39.0	35.0	41.0	33.0	41.0
29	37.49310159681536	39.0	35.0	41.0	33.0	41.0
30	37.37855475319257	39.0	35.0	41.0	33.0	41.0
31	37.332086030763115	39.0	35.0	41.0	32.0	41.0
32	37.303560489049836	39.0	35.0	41.0	33.0	41.0
33	37.288770255854715	39.0	35.0	41.0	33.0	41.0
34	37.14220195421248	39.0	35.0	41.0	32.0	41.0
35	37.137230089479374	39.0	35.0	41.0	32.0	41.0
36	37.04761825918506	39.0	35.0	41.0	32.0	41.0
37	36.9610884173528	39.0	35.0	41.0	32.0	41.0
38	36.872692352269155	39.0	35.0	41.0	31.0	41.0
39	36.80184209717134	39.0	35.0	41.0	31.0	41.0
40	36.66354052590124	39.0	35.0	41.0	31.0	41.0
41	36.48510214556541	39.0	35.0	41.0	31.0	41.0
42	36.537093848086116	39.0	35.0	41.0	31.0	41.0
43	36.5168539591607	38.0	35.0	41.0	31.0	41.0
44	36.425848966008246	38.0	35.0	41.0	31.0	41.0
45	36.31616045262418	38.0	35.0	40.0	31.0	41.0
46	36.27980339610056	38.0	35.0	40.0	31.0	41.0
47	36.19114005189473	38.0	35.0	40.0	31.0	41.0
48	36.10752426208492	38.0	35.0	40.0	31.0	41.0
49	36.02584801988746	37.0	35.0	40.0	31.0	41.0
50	35.85335600869485	37.0	35.0	40.0	30.0	41.0
51	34.755030405956774	36.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	9.0
10	8.0
11	15.0
12	10.0
13	12.0
14	14.0
15	20.0
16	36.0
17	63.0
18	106.0
19	181.0
20	289.0
21	508.0
22	757.0
23	1024.0
24	1429.0
25	2067.0
26	2713.0
27	3425.0
28	4276.0
29	5215.0
30	6814.0
31	8658.0
32	11910.0
33	18586.0
34	34918.0
35	33243.0
36	31865.0
37	49216.0
38	82724.0
39	122571.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42221349688608	22.035389648019414	24.518483652215462	10.023913202879045
2	32.29961753067205	23.86613336991667	25.847783357262305	17.986465742148972
3	33.98986231577255	22.577043798296508	26.144155693636627	17.28893819229432
4	30.313709999787125	24.5497056381703	24.79664316344946	20.339941198593117
5	25.85984639729031	29.26256980597428	24.69635436007938	20.181229436656032
6	27.22155073927513	33.056750690076846	24.00828801808983	15.713410552558194
7	80.39519465252532	6.25362186863586	8.171172172695428	5.180011306143398
8	81.44846361810781	4.857857178336673	8.166678098959505	5.527001104596018
9	75.36987409497632	7.004132182535083	10.735396034334723	6.890597688153859
10	47.77176728267014	20.265907247048695	16.988781372773957	14.973544097507208
11	34.59159513599304	22.67473076950369	21.953077139593027	20.780596954910248
12	31.632838906378986	19.941387817275693	28.070221084774786	20.355552191570535
13	29.17836505597487	20.52112332920982	30.953287651468024	19.347223963347282
14	22.226506046894475	23.682585937333688	33.39073132771495	20.70017668805688
15	20.628034978085477	23.298460898010546	35.330042409864255	20.743461714039725
16	27.496398827756348	23.190366598151755	28.392848272974767	20.920386301117134
17	28.127461392358654	22.752549204194153	25.728808668358642	23.391180735088547
18	27.770300795451053	23.287343978769048	28.716185051764633	20.226170174015266
19	27.89613486005691	24.223057436627645	26.0485974941991	21.832210209116347
20	25.33995302510295	28.384096655699548	26.25508835585495	20.02086196334255
21	25.162791907828915	24.745079580584655	30.992315133911568	19.09981337767486
22	23.998353749831473	21.390844862209335	31.04364218657975	23.567159201379447
23	23.444400029329746	22.90274587905265	30.727874374081964	22.92497971753564
24	24.517301001232322	25.444735902208954	26.844994666244066	23.192968430314657
25	26.281579737877237	24.086342982976923	26.076744587597776	23.555332691548067
26	22.90369199983916	24.918929275105906	25.988755354452326	26.18862337060261
27	23.99740762904496	23.934490596742034	29.33707681791196	22.731024956301045
28	21.332894964035585	24.417485258255496	27.4968718881496	26.75274788955932
29	24.404003037047726	25.957060308104236	26.32439170346682	23.31454495138122
30	28.271744812301463	23.604057912053342	25.780608781420078	22.343588494225113
31	26.519765645881183	23.763006204187057	26.29009482495583	23.427133324975934
32	29.50099224417485	23.879142530731183	23.427842915565815	23.192022309528145
33	28.730613393758915	23.068317016691935	25.13795623718302	23.06311335236613
34	23.589629570059063	24.152807968229265	28.661310046147044	23.596252415564635
35	24.795460512466324	26.984547482254325	25.13322563325047	23.086766372028887
36	29.688087629707248	24.384607560924266	24.051099983679418	21.876204825689072
37	24.382005728761364	27.166912263854165	25.117378110076423	23.33370389730805
38	24.370179218929984	26.29175053633222	24.68665662201765	24.651413622720145
39	24.163215296880878	24.148313894493338	26.287492992792927	25.40097781583286
40	26.714193467509027	22.634520636077006	27.065204279304318	23.58608161710965
41	22.166663907147708	22.887371416271858	26.54980498085288	28.396159695727558
42	22.322537306725263	22.283509824281715	30.308742865657944	25.085210003335074
43	23.354518554611275	21.993760333412965	29.42956012479333	25.22216098718243
44	24.44303051949127	22.552208127650616	27.349750105847264	25.65501124701085
45	24.050390393089533	22.464455424701796	25.486128686618777	27.999025495589898
46	24.651413622720145	23.341272863600132	26.487834069336465	25.51947944434326
47	21.478597565158157	23.897355355871507	30.04926923995752	24.574777839012818
48	22.233128892400046	25.826968699959078	27.14869943871384	24.791202968927028
49	25.206076933811755	22.89588650335045	28.461442029996757	23.436594532841035
50	22.729842305317906	23.768209868512862	27.25703026876926	26.244917557399965
51	22.20498179900137	23.96500299210699	25.595405637460704	28.234609571430937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	338.5
2	525.0
3	757.0
4	989.0
5	708.0
6	427.0
7	442.5
8	458.0
9	462.5
10	467.0
11	484.0
12	501.0
13	480.5
14	460.0
15	477.0
16	494.0
17	511.0
18	528.0
19	558.0
20	588.0
21	727.5
22	867.0
23	971.5
24	1076.0
25	1213.0
26	1689.0
27	2028.0
28	2479.5
29	2931.0
30	3605.0
31	4279.0
32	4995.0
33	5711.0
34	6527.0
35	7343.0
36	8200.0
37	9057.0
38	10225.5
39	11394.0
40	12759.5
41	14125.0
42	15514.5
43	16904.0
44	19200.5
45	21497.0
46	23786.0
47	26075.0
48	30421.0
49	34767.0
50	39262.0
51	43757.0
52	43885.0
53	44013.0
54	40045.0
55	36077.0
56	34021.0
57	31965.0
58	30081.0
59	28197.0
60	24586.0
61	20975.0
62	18683.0
63	16391.0
64	14598.5
65	12806.0
66	10775.0
67	8744.0
68	7483.5
69	6223.0
70	5035.0
71	3847.0
72	3089.5
73	2332.0
74	1750.5
75	895.0
76	621.0
77	500.0
78	379.0
79	259.5
80	140.0
81	109.5
82	79.0
83	55.0
84	31.0
85	27.0
86	23.0
87	17.0
88	11.0
89	8.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.91174195353737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2591228272657	23.45261862326393
2	10.85256670425802	7.143537498081829
3	4.061410240313544	4.010042573899606
4	2.1833613375473613	2.8743289973075585
5	1.3738381898878942	2.260770399575262
6	0.9684825977280576	1.912466960575042
7	0.7565392409083794	1.7429316992151138
8	0.6277031058517876	1.6527042114582386
9	0.5738957949117497	1.699911927932014
>10	7.2197526690093365	48.09586376252326
>50	0.08197637665856351	1.8009649143189577
>100	0.036998187695457874	1.9895851293941238
>500	0.0029018186427810094	0.6990885946077036
>1k	0.0014509093213905047	0.6651847078473574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1536	0.3633103820199206	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1250	0.2956627457844406	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	892	0.2109849353917768	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	818	0.1934817008413379	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	706	0.16699031881905205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	512	0.12110346067330686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17645152668415412	0.0
2	0.0	0.0	0.0	0.8697215329995104	0.0
3	0.0	0.0	0.0	1.233504975412686	0.0
4	0.0	0.0	0.0	1.7051461874880256	0.0
5	0.0	0.0	0.0	3.0992551664108197	0.0
6	0.0	0.0	0.0	3.856624856012243	0.0
7	0.0	0.0	0.0	4.661064054742549	0.0
8	0.0	0.0	0.0	5.8851078222901325	0.0
9	0.0	0.0	0.0	6.347760886893625	0.0
10	0.0	0.0	0.0	7.346627907251779	0.0
11	0.0	0.0	0.0	8.865388299797294	0.0
12	0.0	0.0	0.0	10.02060178012626	0.0
13	0.0	0.0	0.0	10.495790945151013	0.0
14	0.0	0.0	0.0	10.697787733070943	0.0
15	0.0	0.0	0.0	11.060388524500981	0.0
16	0.0	0.0	0.0	11.753895061012964	0.0
17	0.0	0.0	0.0	12.541540615782713	0.0
18	0.0	0.0	0.0	13.473233060298643	0.0
19	0.0	0.0	0.0	14.048711028693479	0.0
20	0.0	0.0	0.0	14.667237492874527	0.0
21	0.0	0.0	0.0	15.397406209863782	0.0
22	0.0	0.0	0.0	16.125682685280015	0.0
23	0.0	0.0	0.0	16.812329846089803	0.0
24	0.0	0.0	0.0	17.365337445805018	0.0
25	2.3653019662755244E-4	0.0	0.0	17.878844502683435	2.3653019662755244E-4
26	2.3653019662755244E-4	0.0	0.0	18.36562364734294	2.3653019662755244E-4
27	2.3653019662755244E-4	0.0	0.0	19.00803966138337	2.3653019662755244E-4
28	2.3653019662755244E-4	0.0	0.0	19.530534865733635	2.3653019662755244E-4
29	2.3653019662755244E-4	0.0	0.0	20.119021994942983	2.3653019662755244E-4
30	2.3653019662755244E-4	0.0	0.0	20.879466577100565	2.3653019662755244E-4
31	2.3653019662755244E-4	0.0	0.0	21.443827626253906	2.3653019662755244E-4
32	2.3653019662755244E-4	0.0	0.0	21.91807067049215	2.3653019662755244E-4
33	2.3653019662755244E-4	0.0	0.0	22.415020613606636	2.3653019662755244E-4
34	2.3653019662755244E-4	0.0	0.0	23.01888220559678	2.3653019662755244E-4
35	4.730603932551049E-4	0.0	0.0	23.62936664309249	2.3653019662755244E-4
36	4.730603932551049E-4	0.0	0.0	24.137906565841728	2.3653019662755244E-4
37	4.730603932551049E-4	0.0	0.0	24.732780010360024	2.3653019662755244E-4
38	4.730603932551049E-4	0.0	0.0	25.419663701366435	2.3653019662755244E-4
39	4.730603932551049E-4	0.0	0.0	26.809515136749933	2.3653019662755244E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.02822E-4	45.000004	25
ATTAGCG	20	7.02822E-4	45.000004	1
CAATTCA	20	7.02822E-4	45.000004	32
TCTACTA	20	7.02822E-4	45.000004	22
TCGTAAG	20	7.02822E-4	45.000004	1
CGGCTAT	20	7.02822E-4	45.000004	41
AACTCTA	20	7.02822E-4	45.000004	12
AATACCC	20	7.02822E-4	45.000004	20
TACGTAC	20	7.02822E-4	45.000004	30
TACCGGT	20	7.02822E-4	45.000004	40
TGCCGAA	20	7.02822E-4	45.000004	41
AACACCG	20	7.02822E-4	45.000004	37
CGGGTTA	25	3.8866077E-5	45.0	6
TACGGAA	25	3.8866077E-5	45.0	5
CCATACG	25	3.8866077E-5	45.0	2
ATACGGA	25	3.8866077E-5	45.0	4
CGCGGGT	55	6.002665E-11	40.909092	4
CGTTTGG	55	6.002665E-11	40.909092	2
CGGGTAG	45	1.9233994E-8	40.0	6
TAGCACG	45	1.9233994E-8	40.0	1
>>END_MODULE