##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935930.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537337
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.197806962855715	33.0	31.0	34.0	30.0	34.0
2	32.318338398435245	34.0	31.0	34.0	30.0	34.0
3	32.36184740674847	34.0	31.0	34.0	30.0	34.0
4	35.895339423862495	37.0	35.0	37.0	35.0	37.0
5	35.90866997805846	37.0	35.0	37.0	35.0	37.0
6	35.9245873632376	37.0	35.0	37.0	35.0	37.0
7	36.07433696172048	37.0	35.0	37.0	35.0	37.0
8	35.78863357632175	37.0	35.0	37.0	35.0	37.0
9	37.53231584647995	39.0	37.0	39.0	35.0	39.0
10	37.260821421193775	39.0	37.0	39.0	34.0	39.0
11	37.19840993640862	39.0	37.0	39.0	34.0	39.0
12	37.09481386913613	39.0	37.0	39.0	34.0	39.0
13	37.09026365204704	39.0	37.0	39.0	33.0	39.0
14	38.28068232785012	40.0	37.0	41.0	34.0	41.0
15	38.43026629470891	40.0	38.0	41.0	34.0	41.0
16	38.51096239417721	40.0	38.0	41.0	34.0	41.0
17	38.44629161959813	40.0	38.0	41.0	34.0	41.0
18	38.44176745692182	40.0	38.0	41.0	34.0	41.0
19	38.451988230849544	40.0	37.0	41.0	34.0	41.0
20	38.32770495982968	40.0	37.0	41.0	34.0	41.0
21	38.31636012409344	40.0	37.0	41.0	34.0	41.0
22	38.29291859670933	40.0	37.0	41.0	34.0	41.0
23	38.28755324870612	40.0	37.0	41.0	34.0	41.0
24	38.205265969028744	40.0	37.0	41.0	34.0	41.0
25	38.13426583317359	40.0	37.0	41.0	34.0	41.0
26	37.97864468666777	40.0	36.0	41.0	34.0	41.0
27	37.94550161258205	40.0	36.0	41.0	34.0	41.0
28	37.93464250554121	40.0	36.0	41.0	34.0	41.0
29	37.902256870455595	40.0	36.0	41.0	34.0	41.0
30	37.790314085946065	40.0	36.0	41.0	33.0	41.0
31	37.81046903526092	40.0	36.0	41.0	33.0	41.0
32	37.74876101962084	40.0	36.0	41.0	33.0	41.0
33	37.68167276774166	40.0	36.0	41.0	33.0	41.0
34	37.6031280183572	40.0	36.0	41.0	33.0	41.0
35	37.613002640800836	40.0	36.0	41.0	33.0	41.0
36	37.522711073311534	40.0	35.0	41.0	33.0	41.0
37	37.514403437693666	40.0	35.0	41.0	33.0	41.0
38	37.422375529695515	40.0	35.0	41.0	33.0	41.0
39	37.36234430162077	40.0	35.0	41.0	33.0	41.0
40	37.26778911558296	40.0	35.0	41.0	33.0	41.0
41	37.20487329180756	39.0	35.0	41.0	33.0	41.0
42	37.20162021226902	39.0	35.0	41.0	33.0	41.0
43	37.14301453277924	39.0	35.0	41.0	32.0	41.0
44	37.04946616369243	39.0	35.0	41.0	32.0	41.0
45	36.92360287864041	39.0	35.0	41.0	32.0	41.0
46	36.838907426810366	39.0	35.0	41.0	31.0	41.0
47	36.777755858986076	39.0	35.0	41.0	31.0	41.0
48	36.72146716120424	39.0	35.0	41.0	31.0	41.0
49	36.64912150103194	39.0	35.0	41.0	31.0	41.0
50	36.527123574218784	39.0	35.0	41.0	31.0	41.0
51	35.54474007931708	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	8.0
10	17.0
11	18.0
12	12.0
13	17.0
14	25.0
15	25.0
16	47.0
17	70.0
18	116.0
19	195.0
20	341.0
21	518.0
22	774.0
23	1240.0
24	1623.0
25	2340.0
26	3012.0
27	3934.0
28	4658.0
29	5493.0
30	6829.0
31	8835.0
32	11584.0
33	16569.0
34	33288.0
35	42593.0
36	36910.0
37	55754.0
38	104159.0
39	196138.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93916108512907	21.873796146552348	24.483145586475526	10.703897181843052
2	31.782103223861373	25.526810921265426	24.35808440513123	18.333001449741968
3	32.80008635176807	24.385813744447155	25.636983866735402	17.177116037049377
4	30.706800387838545	25.276874661525262	24.730104199040824	19.28622075159537
5	26.53139463688523	30.4473728777285	22.6195106609074	20.401721824478866
6	26.41303316168438	34.928545772950685	23.34493995388369	15.31348111148125
7	80.16961422719821	6.5487766522685025	8.30484407364466	4.976765046888638
8	80.79063976610581	6.413479808760609	7.544241323415286	5.251639101718288
9	74.54539702272504	8.587534452308327	9.653904346806566	7.213164178160075
10	42.308085987006294	26.24740153758256	16.938345954214952	14.506166521196196
11	31.555429832674843	26.24516830220141	23.23551142020743	18.963890444916316
12	30.33180294675408	22.449226463094853	27.087656349739547	20.13131424041151
13	26.061856897998837	23.4938223126269	28.523254493920945	21.92106629545332
14	20.20314997850511	27.34540893331373	29.070397162302243	23.381043925878917
15	20.639375289622713	26.994232669628186	31.808902048435154	20.557489992313947
16	23.679180849262195	25.744179165030513	30.0958244081461	20.480815577561195
17	22.417216755965065	26.05701822133968	27.887154616190585	23.63861040650467
18	23.140040607663348	26.566940300035174	28.399496033215655	21.893523059085826
19	24.544931765353958	27.964015133891767	26.001745645656264	21.48930745509801
20	27.478472541440475	26.70279545238835	26.376743086740724	19.44198891943045
21	25.042198843556278	27.80061674517109	26.863402296882587	20.293782114390037
22	23.19773252167634	25.43320113820563	27.05769377504248	24.311372565075548
23	23.771674014631415	26.007142631160708	27.52592879328987	22.695254560918006
24	24.080977114920433	25.09058561014782	27.92214197049524	22.906295304436508
25	23.32986561506094	26.47388882582067	25.84597747782118	24.350268081297212
26	22.441782345157694	27.80526931888182	26.31197926068743	23.44096907527306
27	23.43557208976862	27.358250036755333	26.435179412547434	22.770998460928617
28	20.81766191421771	28.910348626653292	27.389329229142977	22.882660229986023
29	24.19021954564826	26.36241315971169	27.10235848266544	22.34500881197461
30	24.901691117492376	25.846721889614898	27.056577157351903	22.195009835540823
31	24.607648458974534	27.544539088132776	25.082583183365376	22.765229269527318
32	26.523578313051217	27.160236499626862	24.847162953602677	21.469022233719247
33	25.286179808946713	26.89373707747652	25.950009025992998	21.870074087583767
34	23.255238332740905	26.30379073095655	28.15123470001135	22.28973623629119
35	24.489100880825255	25.83257806553429	26.80645479466331	22.871866258977143
36	23.43315275143904	28.822880240891656	25.7274298996719	22.0165371079974
37	24.029240495257167	28.67288126445787	26.547957799295414	20.74992044098955
38	22.95319324744062	28.71642935439026	25.176937378218884	23.153440019950235
39	24.555539633414412	24.68599780026315	27.235422090792184	23.523040475530255
40	23.73259239546132	24.376322494077275	29.519835782758307	22.3712493277031
41	20.932115227501548	27.279156283673	27.772887405855172	24.015841082970276
42	22.315790649071253	26.53325566636952	26.72252236492183	24.428431319637397
43	23.74822504312936	24.982645900058994	26.737038394899294	24.532090661912356
44	22.517340142219876	25.832205859637437	26.12829565058799	25.5221583475547
45	23.756041366963377	24.881591999062042	26.698701187522918	24.66366544645167
46	23.110450238863134	27.100869659078008	26.781889205470684	23.006790896588175
47	21.99252982765006	25.491451361063916	29.16661238664004	23.349406424645984
48	22.511943156715432	24.584757796317767	28.90178789102556	24.001511155941245
49	22.705676326030034	24.42582587835939	29.151910253714146	23.716587541896423
50	20.853951989161366	24.449460952809876	29.1266002527278	25.569986805300953
51	20.723493822312626	24.01677159771242	28.390563091691064	26.86917148828389
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	591.5
2	935.0
3	1317.0
4	1699.0
5	1236.5
6	774.0
7	785.5
8	797.0
9	795.0
10	793.0
11	768.0
12	743.0
13	752.0
14	761.0
15	769.0
16	777.0
17	809.0
18	841.0
19	936.0
20	1031.0
21	1173.5
22	1316.0
23	1606.0
24	1896.0
25	2330.5
26	3707.5
27	4650.0
28	5366.5
29	6083.0
30	7236.0
31	8389.0
32	9685.5
33	10982.0
34	12821.5
35	14661.0
36	15046.5
37	15432.0
38	17416.0
39	19400.0
40	21840.0
41	24280.0
42	25739.0
43	27198.0
44	30792.5
45	34387.0
46	39452.0
47	44517.0
48	47139.0
49	49761.0
50	49276.5
51	48792.0
52	43571.0
53	38350.0
54	34790.5
55	31231.0
56	29511.5
57	27792.0
58	27507.0
59	27222.0
60	26741.0
61	26260.0
62	24502.0
63	22744.0
64	18678.5
65	14613.0
66	11694.0
67	8775.0
68	7389.5
69	6004.0
70	5054.5
71	4105.0
72	3403.5
73	2702.0
74	2249.5
75	1367.0
76	937.0
77	675.0
78	413.0
79	344.5
80	276.0
81	171.0
82	66.0
83	65.5
84	65.0
85	48.5
86	32.0
87	32.5
88	33.0
89	19.0
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	537337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.52683212923902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86901150875545	21.634064174928856
2	10.366947920978427	6.329401577525437
3	3.61676941634729	3.3122553846899847
4	1.887765220008961	2.3050996788251803
5	1.2339127719488259	1.8833724025702894
6	0.9970993250349336	1.8262970226911368
7	0.8646839166478506	1.8477242537853285
8	0.7426237171230444	1.8135959638245325
9	0.7060360743641327	1.9397740247370718
>10	8.565938524794332	51.57865936159601
>50	0.10050203079118977	2.0960333755452676
>100	0.043160381321369845	2.215930399631325
>500	0.004932615008156554	0.991550271405574
>1k	6.165768760195692E-4	0.22624210824402047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1202	0.2236957440116724	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	974	0.18126427176985765	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	731	0.13604125530160774	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	730	0.13585515235317874	TruSeq Adapter, Index 27 (95% over 23bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	639	0.11891978404613864	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	578	0.10756750419196892	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	578	0.10756750419196892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14255485849662317	0.0
2	0.0	0.0	0.0	0.7014220126289461	0.0
3	0.0	0.0	0.0	1.0023504802386585	0.0
4	0.0	0.0	0.0	1.4285262321410959	0.0
5	0.0	0.0	0.0	2.71803356180572	0.0
6	0.0	0.0	0.0	3.424666456990678	0.0
7	0.0	0.0	0.0	4.097428615561556	0.0
8	0.0	0.0	0.0	5.150585200721335	0.0
9	0.0	0.0	0.0	5.5414013924222605	0.0
10	0.0	0.0	0.0	6.481035179040341	0.0
11	0.0	0.0	0.0	7.779847656126416	0.0
12	0.0	0.0	0.0	8.799505710568972	0.0
13	0.0	0.0	0.0	9.22549535952298	0.0
14	1.8610294842901195E-4	0.0	0.0	9.406945734241267	0.0
15	1.8610294842901195E-4	0.0	0.0	9.756447071390951	0.0
16	1.8610294842901195E-4	0.0	0.0	10.410785038067358	0.0
17	1.8610294842901195E-4	0.0	0.0	11.185531612377336	0.0
18	1.8610294842901195E-4	0.0	0.0	12.001406938290124	0.0
19	1.8610294842901195E-4	0.0	0.0	12.55208556269157	0.0
20	1.8610294842901195E-4	0.0	0.0	13.079873524436248	0.0
21	1.8610294842901195E-4	0.0	0.0	13.731792152783077	0.0
22	1.8610294842901195E-4	0.0	0.0	14.429678209391872	0.0
23	1.8610294842901195E-4	0.0	0.0	15.097043382458308	0.0
24	1.8610294842901195E-4	0.0	0.0	15.632833770985433	0.0
25	1.8610294842901195E-4	0.0	0.0	16.112793274983854	0.0
26	1.8610294842901195E-4	0.0	0.0	16.536921894453574	0.0
27	1.8610294842901195E-4	0.0	0.0	16.95397860188299	0.0
28	1.8610294842901195E-4	0.0	0.0	17.405278251823344	0.0
29	1.8610294842901195E-4	0.0	0.0	17.86699966687572	0.0
30	1.8610294842901195E-4	0.0	0.0	18.41321182051487	0.0
31	1.8610294842901195E-4	0.0	0.0	18.884238382988702	0.0
32	1.8610294842901195E-4	0.0	0.0	19.35582325430782	0.0
33	1.8610294842901195E-4	0.0	0.0	19.81903349294763	0.0
34	1.8610294842901195E-4	0.0	0.0	20.266424980970974	0.0
35	3.722058968580239E-4	0.0	0.0	20.748059411505256	0.0
36	3.722058968580239E-4	0.0	0.0	21.228577224348964	0.0
37	3.722058968580239E-4	0.0	0.0	21.71318930205811	0.0
38	3.722058968580239E-4	0.0	0.0	22.219761527681882	0.0
39	3.722058968580239E-4	0.0	0.0	22.70642073782375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.0299284E-4	45.000004	1
CGCATGG	50	2.1827873E-11	45.000004	2
AATTGCG	20	7.0299284E-4	45.000004	1
CGCTAGT	45	3.8380676E-10	45.000004	4
ACTACGG	25	3.8880222E-5	45.000004	2
CGTAAGG	45	3.8380676E-10	45.000004	2
ACGTATT	45	3.8380676E-10	45.000004	26
GCGCTAG	25	3.8880222E-5	45.000004	1
TCGACGT	25	3.8880222E-5	45.000004	26
ATGCACG	20	7.0299284E-4	45.000004	1
ACGCTAG	30	2.163335E-6	44.999996	1
TCCGCTA	55	6.002665E-11	40.909092	2
TAACGGG	160	0.0	40.781254	3
TTAACGG	45	1.9250365E-8	40.000004	2
GCGTTAG	40	3.4536424E-7	39.375004	1
TAGGGCG	80	0.0	39.375004	5
ACGGTCT	40	3.4536424E-7	39.375004	30
TAAGGGA	275	0.0	39.272728	4
CGAATAT	155	0.0	39.193546	14
GCTACGA	155	0.0	39.193546	10
>>END_MODULE