SRR2935929.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935929.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680621
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	6088	0.894477249453073	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	5463	0.8026493452303117	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	5097	0.7488749245174627	No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2849	0.418588318609035	TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1476	0.21686077861247302	TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1303	0.19144281472361271	No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	1216	0.17866037045580432	TruSeq Adapter, Index 16 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	1146	0.16837564518285508	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT	1143	0.1679348712425858	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1141	0.167641021949073	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1128	0.16573100154123954	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT	1106	0.16249865931259835	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC	813	0.11944973781296787	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA	782	0.1148950737635189	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	735	0.10798961536596725	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	40	6.8084773E-9	45.000004	24
GTTCGCA	20	7.0312555E-4	45.000004	23
CATCGTT	20	7.0312555E-4	45.000004	36
TATAGCG	20	7.0312555E-4	45.000004	1
CGGTCTA	40	6.8084773E-9	45.000004	31
TAGGACG	20	7.0312555E-4	45.000004	1
TATCGAG	20	7.0312555E-4	45.000004	1
CGAATAT	190	0.0	45.0	14
TCGACCA	35	1.2109558E-7	45.0	14
TAATGCG	35	1.2109558E-7	45.0	1
GCGTTAG	65	0.0	44.999996	1
TCGACGT	30	2.1641918E-6	44.999996	26
GCTACGA	190	0.0	43.81579	10
TACGAAT	195	0.0	42.692303	12
CGTTAGG	85	0.0	42.35294	2
CTACGAA	200	0.0	41.625	11
CGATGAA	740	0.0	41.351353	19
AACCCGC	50	1.0804797E-9	40.5	21
CGTAAGG	50	1.0804797E-9	40.5	2
TACGGCT	720	0.0	39.6875	7