##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935929.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680621
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97905883009781	33.0	31.0	34.0	30.0	34.0
2	32.10062428282407	33.0	31.0	34.0	30.0	34.0
3	32.17771123723776	34.0	31.0	34.0	30.0	34.0
4	35.7653025692713	37.0	35.0	37.0	35.0	37.0
5	35.77529197600427	37.0	35.0	37.0	35.0	37.0
6	35.78330524623836	37.0	35.0	37.0	35.0	37.0
7	35.949619538627225	37.0	35.0	37.0	35.0	37.0
8	35.55204879073669	37.0	35.0	37.0	33.0	37.0
9	37.36285245386199	39.0	37.0	39.0	34.0	39.0
10	37.12099244660391	39.0	37.0	39.0	33.0	39.0
11	37.05162344388434	39.0	37.0	39.0	33.0	39.0
12	36.85319289296099	39.0	35.0	39.0	33.0	39.0
13	36.73582066965316	39.0	35.0	39.0	33.0	39.0
14	37.63018772562116	40.0	36.0	41.0	32.0	41.0
15	37.80496634690966	40.0	36.0	41.0	33.0	41.0
16	38.0357893747034	40.0	37.0	41.0	33.0	41.0
17	37.996021280565834	40.0	36.0	41.0	33.0	41.0
18	38.01898707210033	40.0	36.0	41.0	33.0	41.0
19	38.03736293767016	40.0	36.0	41.0	34.0	41.0
20	37.799583027852506	40.0	36.0	41.0	33.0	41.0
21	37.8425658332611	40.0	36.0	41.0	33.0	41.0
22	37.856027069396916	40.0	36.0	41.0	33.0	41.0
23	37.82973490385986	40.0	36.0	41.0	33.0	41.0
24	37.76145167427981	40.0	36.0	41.0	33.0	41.0
25	37.73721057681147	40.0	35.0	41.0	33.0	41.0
26	37.55399701155269	40.0	35.0	41.0	33.0	41.0
27	37.4739245483169	39.0	35.0	41.0	33.0	41.0
28	37.45536796543157	39.0	35.0	41.0	33.0	41.0
29	37.427462567273125	39.0	35.0	41.0	33.0	41.0
30	37.32816942174867	39.0	35.0	41.0	32.0	41.0
31	37.301105901816136	39.0	35.0	41.0	32.0	41.0
32	37.198825778223124	39.0	35.0	41.0	32.0	41.0
33	37.1462370394096	39.0	35.0	41.0	32.0	41.0
34	37.05621924683488	39.0	35.0	41.0	31.0	41.0
35	37.01962031732785	40.0	35.0	41.0	31.0	41.0
36	36.943525104279765	39.0	35.0	41.0	31.0	41.0
37	36.877309104479586	39.0	35.0	41.0	31.0	41.0
38	36.824918713939184	39.0	35.0	41.0	31.0	41.0
39	36.72783384585548	39.0	35.0	41.0	31.0	41.0
40	36.63608381169549	39.0	35.0	41.0	31.0	41.0
41	36.55881173222689	39.0	35.0	41.0	30.0	41.0
42	36.51332680008404	39.0	35.0	41.0	31.0	41.0
43	36.459592049025815	39.0	35.0	41.0	31.0	41.0
44	36.414547890823236	39.0	35.0	41.0	31.0	41.0
45	36.29202302015366	39.0	35.0	41.0	30.0	41.0
46	36.189548956026925	38.0	35.0	41.0	30.0	41.0
47	36.11245024764149	38.0	35.0	40.0	30.0	41.0
48	36.08810630292042	38.0	35.0	40.0	30.0	41.0
49	36.09186169689151	38.0	35.0	40.0	30.0	41.0
50	35.94673100007199	38.0	35.0	40.0	30.0	41.0
51	34.93084550726469	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	8.0
10	23.0
11	15.0
12	14.0
13	16.0
14	18.0
15	34.0
16	56.0
17	104.0
18	172.0
19	316.0
20	712.0
21	1216.0
22	1990.0
23	2836.0
24	3772.0
25	4677.0
26	5441.0
27	6247.0
28	7123.0
29	8541.0
30	10689.0
31	13736.0
32	18373.0
33	27429.0
34	49937.0
35	51844.0
36	48842.0
37	73057.0
38	127583.0
39	215623.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.42363371097865	20.493637428172214	22.773320247244797	10.309408613604342
2	30.611162453112673	27.733643246388223	23.34897101323644	18.306223287262664
3	30.738693046497247	27.326661974872945	24.96675829867136	16.96788667995845
4	27.65356931390598	23.959589845156117	29.260190326187406	19.1266505147505
5	28.5492219605331	27.289783888537084	23.87540202256469	20.285592128365128
6	25.098255857518353	34.93618327968135	24.31338439454557	15.652176468254725
7	75.84984888800082	5.879042815311311	12.510192897368727	5.760915399319151
8	75.18942260083071	8.693090574636987	9.695998213396296	6.421488611136008
9	69.98505776342488	7.276296205964846	11.301590753150432	11.43705527745985
10	43.44738114163389	23.826329190548044	18.17913346781836	14.547156199999705
11	33.69129074771422	22.46345616723551	24.144567975422447	19.700685109627823
12	29.363184503563655	20.212864428220698	28.72479691340702	21.69915415480862
13	28.00324997318625	21.8462257262118	31.266446377646297	18.88407792295565
14	21.638327351051466	25.939105610905333	30.27088497122481	22.15168206681839
15	19.242427136394557	23.35514184840021	35.4352863047129	21.967144710492327
16	24.254467611196244	24.853332471375406	27.90716125420755	22.985038663220795
17	24.014539663043017	26.291871687767493	27.469472731520185	22.224115917669305
18	25.214032479162412	23.563627922147568	28.911244290140914	22.311095308549103
19	23.546143889183554	26.465536620233582	26.338446800789278	23.649872689793586
20	24.961322086741372	27.81063176128859	27.93125689627561	19.29678925569443
21	23.101109134158364	28.534823345150972	29.384488577343337	18.979578943347324
22	22.79726896466609	22.40850634934861	31.401470128015447	23.392754557969855
23	22.239543005578728	26.241182684636534	29.78280129469999	21.736473015084755
24	23.07378114986167	25.08958730335973	28.303857800449883	23.53277374632872
25	22.226319787370652	28.553482775289034	25.51684417612739	23.70335326121292
26	20.77543890065102	24.370685006780572	27.856619175723345	26.997256916845057
27	23.036168440292027	24.851275526320816	28.38025861676322	23.732297416623936
28	18.080987803785074	26.841369866636498	28.16633633108588	26.91130599849255
29	21.126294957105348	24.408297716350216	27.341795213488858	27.12361211305558
30	23.117858543888595	25.42971786060083	28.49897373134241	22.953449864168164
31	22.88101601331725	25.609406703583932	25.13998245719718	26.369594825901636
32	24.251529118261118	24.525543584461836	25.02993589677662	26.192991400500425
33	23.120356262883455	23.132551008564235	25.209624739759718	28.537467988792585
34	19.843495866275063	23.439623520285153	27.919355999888335	28.797524613551445
35	19.842467393747768	24.127083942458434	29.076093743801618	26.954354919992184
36	20.861977517590553	26.50623474738511	24.672909005158523	27.958878729865816
37	18.8886325870051	26.364452463265163	28.086850097190652	26.66006485253908
38	20.314977057716412	26.09249494211904	25.32731138181161	28.265216618352945
39	21.256176344838025	22.641381914457533	24.729915768100014	31.372525972604432
40	22.36986516725167	20.654813765664002	28.90815887255903	28.06716219452529
41	18.813994866452845	22.696331732344433	26.512552507195636	31.97712089400709
42	19.66968400916222	21.819044666561858	31.17167998048841	27.33959134378751
43	21.210629704343535	22.4105632944032	27.899521172576218	28.47928582867705
44	21.39825247825148	21.997998886311176	27.2866984709552	29.317050164482144
45	20.21697831832988	21.251621680788574	27.04353818057333	31.487861820308215
46	22.75010615305728	23.029850680481502	26.783187706520955	27.43685545994026
47	17.502986243445324	23.472387716511832	32.28610342613584	26.738522613907005
48	19.361729949560768	23.86790886558011	28.54834041265256	28.22202077220656
49	21.464809343232137	21.212245875457857	30.699317241166526	26.623627540143485
50	20.11001717549121	21.754103972695525	29.596941616553117	28.538937235260153
51	19.31735870624033	21.599833093601283	26.860176221421316	32.22263197873706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	455.5
2	719.0
3	1560.0
4	2401.0
5	1640.5
6	880.0
7	887.0
8	894.0
9	907.5
10	921.0
11	945.0
12	969.0
13	950.0
14	931.0
15	1008.5
16	1086.0
17	1082.0
18	1078.0
19	1114.0
20	1150.0
21	1314.0
22	1478.0
23	1523.0
24	1568.0
25	2160.0
26	3471.0
27	4190.0
28	4816.0
29	5442.0
30	6485.0
31	7528.0
32	9184.5
33	10841.0
34	12914.0
35	14987.0
36	16821.0
37	18655.0
38	19386.5
39	20118.0
40	21916.5
41	23715.0
42	26256.0
43	28797.0
44	31456.0
45	34115.0
46	38053.5
47	41992.0
48	59087.0
49	76182.0
50	74306.5
51	72431.0
52	72370.5
53	72310.0
54	63520.0
55	54730.0
56	49803.0
57	44876.0
58	40552.0
59	36228.0
60	32068.0
61	27908.0
62	24328.0
63	20748.0
64	18073.5
65	15399.0
66	13285.0
67	11171.0
68	9741.5
69	8312.0
70	6776.0
71	5240.0
72	4364.0
73	3488.0
74	2834.5
75	1563.0
76	945.0
77	810.5
78	676.0
79	442.5
80	209.0
81	163.0
82	117.0
83	71.0
84	25.0
85	15.5
86	6.0
87	20.5
88	35.0
89	18.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	680621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.531640445777896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28935536010475	23.191596761862165
2	11.550504014711128	7.515136871481929
3	4.236438613092097	4.134548931951661
4	2.160840615712522	2.8118275988397237
5	1.3721793685392496	2.2319622922216715
6	0.969769265352906	1.8928911033496147
7	0.7348543553203233	1.6734212367106267
8	0.5900432399353773	1.535605962323164
9	0.5169353701544035	1.5135080036011341
>10	6.418221388870274	43.67882845350586
>50	0.1084541109218895	2.3125952186363685
>100	0.044201885536112276	2.591885563912632
>500	0.0027341372496564294	0.5940108358841178
>1k	0.0041012058744846445	1.854226986583116
>5k	0.0013670686248282147	2.4679541791362105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	6088	0.894477249453073	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	5463	0.8026493452303117	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	5097	0.7488749245174627	No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2849	0.418588318609035	TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1476	0.21686077861247302	TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1303	0.19144281472361271	No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	1216	0.17866037045580432	TruSeq Adapter, Index 16 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	1146	0.16837564518285508	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT	1143	0.1679348712425858	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1141	0.167641021949073	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1128	0.16573100154123954	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT	1106	0.16249865931259835	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC	813	0.11944973781296787	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA	782	0.1148950737635189	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	735	0.10798961536596725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.876985870256721E-4	0.0	0.0	0.1889450957287536	0.0
2	7.346232337820902E-4	0.0	0.0	1.0372880061003114	0.0
3	8.815478805385082E-4	0.0	0.0	1.4648387281614879	0.0
4	8.815478805385082E-4	0.0	0.0	2.1922626542525134	0.0
5	8.815478805385082E-4	0.0	0.0	4.6184293461412445	0.0
6	8.815478805385082E-4	0.0	0.0	5.612374581448413	0.0
7	8.815478805385082E-4	0.0	0.0	6.697266173097804	0.0
8	8.815478805385082E-4	0.0	0.0	8.227633293712653	0.0
9	8.815478805385082E-4	0.0	0.0	8.801961737883492	0.0
10	8.815478805385082E-4	0.0	0.0	10.742395547595505	0.0
11	8.815478805385082E-4	0.0	0.0	12.375609920939848	0.0
12	8.815478805385082E-4	0.0	0.0	14.538193796547565	0.0
13	8.815478805385082E-4	0.0	0.0	15.156893484038841	0.0
14	8.815478805385082E-4	0.0	0.0	15.451183551491946	0.0
15	8.815478805385082E-4	0.0	0.0	16.002444826122026	0.0
16	8.815478805385082E-4	0.0	0.0	16.78981400808967	0.0
17	8.815478805385082E-4	0.0	0.0	17.663868731643603	0.0
18	8.815478805385082E-4	0.0	0.0	18.538217304491045	0.0
19	8.815478805385082E-4	0.0	0.0	19.34116049901487	1.4692464675641803E-4
20	8.815478805385082E-4	0.0	0.0	20.008639169229276	1.4692464675641803E-4
21	8.815478805385082E-4	0.0	0.0	20.76412570285078	1.4692464675641803E-4
22	8.815478805385082E-4	0.0	0.0	21.59998001824804	1.4692464675641803E-4
23	8.815478805385082E-4	0.0	0.0	22.292582803057797	1.4692464675641803E-4
24	0.0010284725272949261	0.0	0.0	22.893945382231816	1.4692464675641803E-4
25	0.0010284725272949261	0.0	0.0	23.43932967099164	1.4692464675641803E-4
26	0.0010284725272949261	0.0	0.0	23.936081901675088	1.4692464675641803E-4
27	0.0011753971740513442	0.0	0.0	24.58974965509439	1.4692464675641803E-4
28	0.0013223218208077623	0.0	0.0	25.118825308064253	1.4692464675641803E-4
29	0.0013223218208077623	0.0	0.0	25.68668906777781	1.4692464675641803E-4
30	0.0013223218208077623	0.0	0.0	26.41719841145072	1.4692464675641803E-4
31	0.0014692464675641803	0.0	0.0	26.991233006328045	1.4692464675641803E-4
32	0.0014692464675641803	0.0	0.0	27.51399089948738	1.4692464675641803E-4
33	0.0014692464675641803	0.0	0.0	28.05173510661587	1.4692464675641803E-4
34	0.0014692464675641803	0.0	0.0	28.630765139482914	1.4692464675641803E-4
35	0.0014692464675641803	0.0	0.0	29.25519488819769	1.4692464675641803E-4
36	0.0014692464675641803	0.0	0.0	29.77648353488946	1.4692464675641803E-4
37	0.0016161711143205984	0.0	0.0	30.342878048135454	1.4692464675641803E-4
38	0.0016161711143205984	0.0	0.0	30.986261076281806	1.4692464675641803E-4
39	0.0016161711143205984	0.0	0.0	32.03148301330697	1.4692464675641803E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	40	6.8084773E-9	45.000004	24
GTTCGCA	20	7.0312555E-4	45.000004	23
CATCGTT	20	7.0312555E-4	45.000004	36
TATAGCG	20	7.0312555E-4	45.000004	1
CGGTCTA	40	6.8084773E-9	45.000004	31
TAGGACG	20	7.0312555E-4	45.000004	1
TATCGAG	20	7.0312555E-4	45.000004	1
CGAATAT	190	0.0	45.0	14
TCGACCA	35	1.2109558E-7	45.0	14
TAATGCG	35	1.2109558E-7	45.0	1
GCGTTAG	65	0.0	44.999996	1
TCGACGT	30	2.1641918E-6	44.999996	26
GCTACGA	190	0.0	43.81579	10
TACGAAT	195	0.0	42.692303	12
CGTTAGG	85	0.0	42.35294	2
CTACGAA	200	0.0	41.625	11
CGATGAA	740	0.0	41.351353	19
AACCCGC	50	1.0804797E-9	40.5	21
CGTAAGG	50	1.0804797E-9	40.5	2
TACGGCT	720	0.0	39.6875	7
>>END_MODULE