SRR2935927.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935927.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556201
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1741	0.3130163376189543	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1571	0.28245184744364	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	1222	0.21970474702490647	TruSeq Adapter, Index 22 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	713	0.12819106761764182	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	679	0.12207816958257896	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.1141673603607329	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAG	35	1.2104101E-7	45.000004	1
CGGCCTA	20	7.030142E-4	45.000004	43
CCGATAG	35	1.2104101E-7	45.000004	11
ATTCGAT	20	7.030142E-4	45.000004	14
TGCGATC	30	2.1634733E-6	45.000004	10
CGTAATT	20	7.030142E-4	45.000004	23
AATAGCG	20	7.030142E-4	45.000004	1
TGTAACG	20	7.030142E-4	45.000004	1
TCGATCA	25	3.888201E-5	45.0	17
TAATCCG	25	3.888201E-5	45.0	1
CTACGAA	110	0.0	40.909092	11
TACGAAT	110	0.0	40.909092	12
CGAATAT	110	0.0	40.909092	14
CGGTCTA	50	1.0786607E-9	40.5	31
GCGTAAG	50	1.0786607E-9	40.5	1
CTCACGT	45	1.9252184E-8	40.0	45
ATAGCGG	90	0.0	40.0	2
TACGGGT	80	0.0	39.375004	4
GTGTAAG	80	0.0	39.375004	1
AATATAC	115	0.0	39.130436	16