SRR2935926.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376794
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2112	0.5605184795936241	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1604	0.42569679984288494	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1108	0.29405988418074597	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	672	0.17834678896160766	Illumina PCR Primer Index 2 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	521	0.13827184084672262	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	428	0.11358991916007155	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	418	0.1109359490862381	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	410	0.10881277302717135	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	400	0.1061588029533379	No Hit
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	397	0.10536261193118787	Illumina PCR Primer Index 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCCG	30	2.1616033E-6	45.000004	7
CTACGGG	65	0.0	45.000004	3
GCGTGAA	30	2.1616033E-6	45.000004	36
CGAACCC	25	3.8857976E-5	45.0	19
AATTTAC	20	7.027243E-4	45.0	15
CGTATGG	25	3.8857976E-5	45.0	2
CACGGGT	20	7.027243E-4	45.0	4
AACCCGC	20	7.027243E-4	45.0	21
TGGAACG	20	7.027243E-4	45.0	1
TAACGCG	20	7.027243E-4	45.0	1
TCGATAG	20	7.027243E-4	45.0	1
ACGGGCC	20	7.027243E-4	45.0	5
GTATGCG	25	3.8857976E-5	45.0	1
TTGACGG	40	6.7939254E-9	45.0	2
CCATACG	25	3.8857976E-5	45.0	2
GTTCCGA	20	7.027243E-4	45.0	15
CACACGA	20	7.027243E-4	45.0	27
TAGGACG	20	7.027243E-4	45.0	1
GACCTAT	25	3.8857976E-5	45.0	33
TACGGGA	65	0.0	41.538464	4