##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376794
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08597536054184	33.0	31.0	34.0	30.0	34.0
2	32.19659814115936	33.0	31.0	34.0	30.0	34.0
3	32.27084030000478	34.0	31.0	34.0	30.0	34.0
4	35.83299097119381	37.0	35.0	37.0	35.0	37.0
5	35.867856176053756	37.0	35.0	37.0	35.0	37.0
6	35.88250343689125	37.0	35.0	37.0	35.0	37.0
7	36.04198315259797	37.0	35.0	37.0	35.0	37.0
8	35.71188766275471	37.0	35.0	37.0	35.0	37.0
9	37.45815219987579	39.0	37.0	39.0	34.0	39.0
10	37.220582068716595	39.0	37.0	39.0	34.0	39.0
11	37.15336496865661	39.0	37.0	39.0	34.0	39.0
12	37.01820888867657	39.0	37.0	39.0	34.0	39.0
13	36.95161812555401	39.0	37.0	39.0	33.0	39.0
14	37.92143186993423	40.0	37.0	41.0	33.0	41.0
15	38.13171653476435	40.0	37.0	41.0	33.0	41.0
16	38.29678551144657	40.0	37.0	41.0	34.0	41.0
17	38.31307292578969	40.0	37.0	41.0	34.0	41.0
18	38.31578793717522	40.0	37.0	41.0	34.0	41.0
19	38.32630296660775	40.0	37.0	41.0	34.0	41.0
20	38.20501653423356	40.0	37.0	41.0	34.0	41.0
21	38.17029729772767	40.0	37.0	41.0	34.0	41.0
22	38.206924738716644	40.0	37.0	41.0	34.0	41.0
23	38.238294664989354	40.0	37.0	41.0	34.0	41.0
24	38.15400722941448	40.0	37.0	41.0	34.0	41.0
25	38.07635737299426	40.0	36.0	41.0	34.0	41.0
26	37.95270890725436	40.0	36.0	41.0	34.0	41.0
27	37.889740813282586	40.0	36.0	41.0	34.0	41.0
28	37.9002478808049	40.0	36.0	41.0	34.0	41.0
29	37.92118239674729	40.0	36.0	41.0	34.0	41.0
30	37.8329007362113	40.0	36.0	41.0	34.0	41.0
31	37.8124227031216	40.0	36.0	41.0	33.0	41.0
32	37.78873071227249	40.0	36.0	41.0	33.0	41.0
33	37.75846749152056	40.0	36.0	41.0	33.0	41.0
34	37.668901309468836	40.0	36.0	41.0	33.0	41.0
35	37.69553655312983	40.0	36.0	41.0	33.0	41.0
36	37.61483197715462	40.0	35.0	41.0	33.0	41.0
37	37.557113435988896	40.0	35.0	41.0	33.0	41.0
38	37.49023073615822	40.0	35.0	41.0	33.0	41.0
39	37.45231877365351	40.0	35.0	41.0	33.0	41.0
40	37.3601437390192	40.0	35.0	41.0	33.0	41.0
41	37.27071025547116	39.0	35.0	41.0	33.0	41.0
42	37.27233448515634	39.0	35.0	41.0	33.0	41.0
43	37.236986257742956	39.0	35.0	41.0	33.0	41.0
44	37.16439752225354	39.0	35.0	41.0	33.0	41.0
45	37.082251840528244	39.0	35.0	41.0	32.0	41.0
46	37.01959691502518	39.0	35.0	41.0	32.0	41.0
47	36.970779789487096	39.0	35.0	41.0	32.0	41.0
48	36.94101286113898	39.0	35.0	41.0	32.0	41.0
49	36.932690010987436	39.0	35.0	41.0	32.0	41.0
50	36.82141435373175	39.0	35.0	41.0	32.0	41.0
51	35.84456228071572	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	6.0
11	3.0
12	5.0
13	7.0
14	15.0
15	19.0
16	25.0
17	42.0
18	66.0
19	109.0
20	198.0
21	316.0
22	493.0
23	734.0
24	1001.0
25	1437.0
26	1873.0
27	2475.0
28	2955.0
29	3688.0
30	4831.0
31	6376.0
32	8467.0
33	13025.0
34	25385.0
35	30838.0
36	25565.0
37	38818.0
38	71687.0
39	136214.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13182269356731	21.076503341348324	23.138638088716913	10.65303587636746
2	32.5737670982022	24.224377245922174	24.414401503208648	18.787454152666974
3	33.215231665047746	24.044438074916265	25.00942159376211	17.73090866627388
4	29.5094932509541	24.637069592403275	24.56833176749099	21.28510538915163
5	25.229701109890286	29.067607233660837	24.708195990382013	20.99449566606687
6	26.501748966278655	32.746009755993995	24.788611283619165	15.963629994108185
7	77.61217004517057	6.271065887461052	9.710611103149201	6.406152964219175
8	76.50599531839678	6.1954277403568	10.068100872094567	7.230476069151845
9	70.25961135262239	8.187232280768802	12.843091981294819	8.710064385313991
10	41.29550895184106	23.77797947950339	19.79330881064985	15.133202758005702
11	30.309134434200118	23.747724220661688	25.24270556325207	20.700435781886124
12	28.35342388679225	21.60145862195258	30.11380223676598	19.931315254489192
13	25.63336995812035	23.839551585216327	30.876818632993093	19.650259823670226
14	19.72828654384093	27.403037203352493	30.898050393583766	21.97062585922281
15	18.482778388190894	25.31675132831202	36.00959675578698	20.190873527710103
16	22.297329575311707	26.190438276617993	30.13609558538618	21.37613656268412
17	22.819896282849513	25.1482242286236	27.239021852789584	24.7928576357373
18	24.3992738737878	24.456865024389984	29.841239510183282	21.302621591638932
19	22.8870417257175	26.500952775256508	28.345461976570753	22.26654352245524
20	24.288868718716326	27.703466615710443	27.845984808675293	20.161679856897933
21	22.747708296841246	27.073148723175	30.349209382315006	19.829933597668752
22	21.48494933571129	23.868214462013725	29.8903379565492	24.75649824572578
23	20.796509498558894	25.9911251240731	29.933863065760068	23.278502311607934
24	22.782740701815847	24.52772602536134	29.11564409199722	23.573889180825596
25	21.006969325413888	27.32102952807104	26.944961968608844	24.727039177906228
26	20.10435410330313	26.154609680621242	29.037617371826514	24.70341884424911
27	22.296533384289557	26.15646745967293	28.80088324124057	22.746115914796945
28	17.339182683376062	26.886840023991887	30.526759980254464	25.247217312377586
29	20.314548533150738	24.8310748048005	31.515098435750037	23.33927822629872
30	24.15245465692129	25.164148049066597	29.482422756201	21.200974537811113
31	21.96478712506038	28.606878028843347	27.470182646220483	21.958152199875794
32	23.06061136854621	26.19256145267706	28.185427581118596	22.561399597658134
33	23.225953704146033	24.73898204323848	28.044236373190657	23.99082787942483
34	18.557354947265615	26.150097931495726	31.43813330360887	23.854413817629794
35	19.258799237779794	25.179806472502214	29.964383721609156	25.597010568108836
36	23.09166281841006	26.311459311984798	27.807236845597327	22.789641024007814
37	20.738652950949323	28.265312080340983	28.980822412246482	22.01521255646321
38	19.44882349506627	27.265561553527924	28.301936867359885	24.983678084045923
39	20.703620545974726	24.307711906240545	30.551972695955882	24.43669485182885
40	22.264420346396175	23.185878756031148	30.859302430505792	23.690398467066885
41	19.549143563857175	24.458722803441667	27.327399056248243	28.664734576452915
42	20.254038015467337	23.399257949967357	29.656523193044475	26.69018084152083
43	22.440643959298715	22.660392681412127	29.20216351640419	25.69679984288497
44	21.06827603411944	23.078658365048277	29.641395563623625	26.211670037208663
45	21.018912190746136	22.748504487863393	28.83007691205274	27.40250640933773
46	22.19276315440267	24.220396290811426	29.40837699114105	24.178463563644854
47	18.79249669580726	24.69227216993901	32.1419661671895	24.37326496706423
48	19.940869546754993	23.141822852805515	30.286575688572537	26.630731911866963
49	21.633040865831195	21.33314224748802	32.697707500650225	24.336109386030564
50	19.813213586203602	22.282732739905626	31.68919887259351	26.21485480129726
51	19.275519249244947	22.361821048105863	28.207455532731412	30.15520416991778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	259.5
2	396.0
3	653.0
4	910.0
5	665.0
6	420.0
7	396.5
8	373.0
9	414.5
10	456.0
11	498.5
12	541.0
13	568.0
14	595.0
15	617.5
16	640.0
17	605.5
18	571.0
19	567.0
20	563.0
21	769.5
22	976.0
23	1042.5
24	1109.0
25	1459.5
26	2163.0
27	2516.0
28	3035.5
29	3555.0
30	4178.5
31	4802.0
32	5594.5
33	6387.0
34	7405.0
35	8423.0
36	9574.0
37	10725.0
38	11520.5
39	12316.0
40	14356.5
41	16397.0
42	19659.5
43	22922.0
44	29549.5
45	36177.0
46	41674.5
47	47172.0
48	45890.5
49	44609.0
50	38463.5
51	32318.0
52	28113.0
53	23908.0
54	22352.0
55	20796.0
56	19216.5
57	17637.0
58	16441.5
59	15246.0
60	13582.0
61	11918.0
62	10626.0
63	9334.0
64	8425.5
65	7517.0
66	6111.5
67	4706.0
68	4026.5
69	3347.0
70	2700.0
71	2053.0
72	1635.0
73	1217.0
74	955.5
75	508.5
76	323.0
77	256.0
78	189.0
79	124.0
80	59.0
81	46.5
82	34.0
83	21.5
84	9.0
85	5.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	376794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.18275658250525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.90154765255981	23.195260404952833
2	12.473194652832158	8.27789963942271
3	4.731309297586046	4.709936542150249
4	2.4208333603299237	3.213196964905443
5	1.4078872882651987	2.335879059105374
6	0.9363448525856337	1.864230199237851
7	0.7123417103346656	1.6546223104320472
8	0.5448843573642248	1.4464611996825498
9	0.49416984239824874	1.4758125831644462
>10	6.210082000956744	44.622613824220295
>50	0.1167005903565804	2.5619577092590333
>100	0.046680236142632156	3.0351974737547076
>500	0.0016096633152631777	0.31860893851204275
>1k	0.0024144949728947666	1.2883231512004145
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2112	0.5605184795936241	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1604	0.42569679984288494	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1108	0.29405988418074597	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	672	0.17834678896160766	Illumina PCR Primer Index 2 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	521	0.13827184084672262	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	428	0.11358991916007155	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	418	0.1109359490862381	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	410	0.10881277302717135	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	400	0.1061588029533379	No Hit
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	397	0.10536261193118787	Illumina PCR Primer Index 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6539700738334476E-4	0.0	0.0	0.30467576447607975	0.0
2	2.6539700738334476E-4	0.0	0.0	1.6608544722049714	0.0
3	2.6539700738334476E-4	0.0	0.0	2.309484758249866	0.0
4	5.307940147666895E-4	0.0	0.0	3.17706757538602	0.0
5	5.307940147666895E-4	0.0	0.0	5.508845682256087	0.0
6	5.307940147666895E-4	0.0	0.0	6.608385483845284	0.0
7	5.307940147666895E-4	0.0	0.0	7.799752649989118	0.0
8	5.307940147666895E-4	0.0	0.0	9.509970965567392	0.0
9	5.307940147666895E-4	0.0	0.0	10.14135044613237	0.0
10	5.307940147666895E-4	0.0	0.0	11.601564780755533	0.0
11	5.307940147666895E-4	0.0	0.0	13.734029735080707	0.0
12	5.307940147666895E-4	0.0	0.0	15.479280455633582	0.0
13	5.307940147666895E-4	0.0	0.0	16.181255540162528	0.0
14	5.307940147666895E-4	0.0	0.0	16.46947669018084	0.0
15	5.307940147666895E-4	0.0	0.0	17.03663009495905	0.0
16	5.307940147666895E-4	0.0	0.0	18.14439720377713	0.0
17	5.307940147666895E-4	0.0	0.0	19.384332022272115	0.0
18	5.307940147666895E-4	0.0	0.0	20.710255471159307	0.0
19	5.307940147666895E-4	0.0	0.0	21.538824928210108	0.0
20	5.307940147666895E-4	0.0	0.0	22.329708010212478	0.0
21	5.307940147666895E-4	0.0	0.0	23.379087777406223	0.0
22	5.307940147666895E-4	0.0	0.0	24.41758626729725	0.0
23	5.307940147666895E-4	0.0	0.0	25.33798308890269	0.0
24	5.307940147666895E-4	0.0	0.0	26.07419438738409	0.0
25	5.307940147666895E-4	0.0	0.0	26.735829126790765	0.0
26	5.307940147666895E-4	0.0	0.0	27.362696858230226	0.0
27	5.307940147666895E-4	0.0	0.0	28.07236845597329	0.0
28	5.307940147666895E-4	0.0	0.0	28.737984150490718	0.0
29	5.307940147666895E-4	0.0	0.0	29.425097002606197	0.0
30	5.307940147666895E-4	0.0	0.0	30.18705181080378	0.0
31	5.307940147666895E-4	0.0	0.0	30.844174801084943	0.0
32	5.307940147666895E-4	0.0	0.0	31.456976491133087	0.0
33	5.307940147666895E-4	0.0	0.0	32.04138070139121	0.0
34	5.307940147666895E-4	0.0	0.0	32.68231447422199	0.0
35	5.307940147666895E-4	0.0	0.0	33.346072389687734	0.0
36	5.307940147666895E-4	0.0	0.0	33.97055154806075	0.0
37	5.307940147666895E-4	0.0	0.0	34.58733419321964	0.0
38	5.307940147666895E-4	0.0	0.0	35.26011560693642	0.0
39	5.307940147666895E-4	0.0	0.0	36.15238034575922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCCG	30	2.1616033E-6	45.000004	7
CTACGGG	65	0.0	45.000004	3
GCGTGAA	30	2.1616033E-6	45.000004	36
CGAACCC	25	3.8857976E-5	45.0	19
AATTTAC	20	7.027243E-4	45.0	15
CGTATGG	25	3.8857976E-5	45.0	2
CACGGGT	20	7.027243E-4	45.0	4
AACCCGC	20	7.027243E-4	45.0	21
TGGAACG	20	7.027243E-4	45.0	1
TAACGCG	20	7.027243E-4	45.0	1
TCGATAG	20	7.027243E-4	45.0	1
ACGGGCC	20	7.027243E-4	45.0	5
GTATGCG	25	3.8857976E-5	45.0	1
TTGACGG	40	6.7939254E-9	45.0	2
CCATACG	25	3.8857976E-5	45.0	2
GTTCCGA	20	7.027243E-4	45.0	15
CACACGA	20	7.027243E-4	45.0	27
TAGGACG	20	7.027243E-4	45.0	1
GACCTAT	25	3.8857976E-5	45.0	33
TACGGGA	65	0.0	41.538464	4
>>END_MODULE