##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935925.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227223
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04407564375085	33.0	31.0	34.0	30.0	34.0
2	32.17178718703652	33.0	31.0	34.0	30.0	34.0
3	32.22936498505873	34.0	31.0	34.0	30.0	34.0
4	35.79319875188691	37.0	35.0	37.0	35.0	37.0
5	35.821488141605386	37.0	35.0	37.0	35.0	37.0
6	35.84339173411142	37.0	35.0	37.0	35.0	37.0
7	35.9918978272446	37.0	35.0	37.0	35.0	37.0
8	35.61793040317222	37.0	35.0	37.0	33.0	37.0
9	37.405751178357825	39.0	37.0	39.0	34.0	39.0
10	37.15876033676168	39.0	37.0	39.0	34.0	39.0
11	37.147397050474645	39.0	37.0	39.0	34.0	39.0
12	37.081840306659096	39.0	37.0	39.0	34.0	39.0
13	37.028975939935656	39.0	37.0	39.0	33.0	39.0
14	38.191411080744466	40.0	37.0	41.0	33.0	41.0
15	38.324676639248665	40.0	37.0	41.0	34.0	41.0
16	38.45044295691898	40.0	38.0	41.0	34.0	41.0
17	38.43862637145007	40.0	38.0	41.0	34.0	41.0
18	38.446213631542584	40.0	37.0	41.0	34.0	41.0
19	38.452757863420516	40.0	37.0	41.0	34.0	41.0
20	38.284491446728545	40.0	37.0	41.0	34.0	41.0
21	38.27699220589465	40.0	37.0	41.0	34.0	41.0
22	38.2580416595151	40.0	37.0	41.0	34.0	41.0
23	38.26633307367652	40.0	37.0	41.0	34.0	41.0
24	38.19193919629615	40.0	37.0	41.0	34.0	41.0
25	38.11315755887388	40.0	37.0	41.0	34.0	41.0
26	37.98619858024936	40.0	37.0	41.0	34.0	41.0
27	37.95588474758277	40.0	36.0	41.0	34.0	41.0
28	37.93968040207197	40.0	36.0	41.0	34.0	41.0
29	37.9510568912478	40.0	36.0	41.0	34.0	41.0
30	37.856563816162975	40.0	36.0	41.0	33.0	41.0
31	37.8742204794409	40.0	36.0	41.0	33.0	41.0
32	37.82821281296348	40.0	36.0	41.0	33.0	41.0
33	37.81636542075406	40.0	36.0	41.0	33.0	41.0
34	37.78010148620518	40.0	36.0	41.0	33.0	41.0
35	37.755601325570034	40.0	36.0	41.0	33.0	41.0
36	37.71141125678298	40.0	36.0	41.0	33.0	41.0
37	37.63838167791113	40.0	36.0	41.0	33.0	41.0
38	37.56914572908553	40.0	36.0	41.0	33.0	41.0
39	37.53580403392262	40.0	36.0	41.0	33.0	41.0
40	37.45553927199271	40.0	36.0	41.0	33.0	41.0
41	37.385462739247345	40.0	35.0	41.0	33.0	41.0
42	37.376071084353256	40.0	35.0	41.0	33.0	41.0
43	37.35551418650401	40.0	35.0	41.0	33.0	41.0
44	37.26200252615272	39.0	35.0	41.0	33.0	41.0
45	37.16411630864833	39.0	35.0	41.0	32.0	41.0
46	37.10841772179753	39.0	35.0	41.0	32.0	41.0
47	37.06049563644525	39.0	35.0	41.0	32.0	41.0
48	37.04252650479925	39.0	35.0	41.0	32.0	41.0
49	37.0411710082166	39.0	35.0	41.0	32.0	41.0
50	36.93367308767158	39.0	35.0	41.0	32.0	41.0
51	35.990819591326584	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	6.0
11	2.0
12	5.0
13	13.0
14	6.0
15	9.0
16	21.0
17	24.0
18	37.0
19	68.0
20	121.0
21	234.0
22	318.0
23	551.0
24	722.0
25	912.0
26	1285.0
27	1519.0
28	1852.0
29	2131.0
30	2781.0
31	3592.0
32	4808.0
33	7022.0
34	13067.0
35	18482.0
36	15523.0
37	23448.0
38	43555.0
39	85024.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.409676837292	21.628092226579177	23.13058097111648	10.831649965012344
2	31.31593192590539	26.564212249640224	23.854979469507928	18.264876354946463
3	31.760429181905003	25.478054598346116	25.62944772316183	17.132068496587053
4	30.180483489787562	24.405099835844084	26.80978598117268	18.60463069319567
5	25.932233972793245	29.96571649876993	23.357230562046976	20.744818966389847
6	25.03707811269106	35.08051561681696	24.064465305008735	15.817940965483249
7	75.74893386673004	7.134401006940319	10.949155675261748	6.167509451067894
8	74.97392429463567	7.689362432500231	10.160943214375305	7.175770058488798
9	68.76592598460543	8.624567055271694	12.759711824947297	9.849795135175576
10	38.55199517654463	26.46563067999278	19.857584839562897	15.124789303899695
11	29.596035612592036	24.63527019711913	25.496538642655015	20.272155547633822
12	27.519661301892857	22.389458813588416	29.704739396980056	20.386140487538672
13	24.23698305189175	25.38299379904323	30.86527332180281	19.514749827262204
14	18.880130972656815	29.876817047570007	28.39237225104853	22.850679728724646
15	17.164195525981086	27.388072510265243	34.89391478855573	20.553817175197935
16	19.519590886485965	26.520642716626398	31.293046918665805	22.666719478221836
17	19.350153813654426	26.933453039525045	28.48435237629993	25.23204077052059
18	20.219343992465554	27.35858605862963	30.059457009193615	22.36261293971121
19	21.4960633386585	28.055698586850802	27.965918943064743	22.482319131425957
20	23.876104091575236	27.679856352569942	28.77745650748384	19.666583048370985
21	22.389458813588416	28.273986348212986	29.28840830373686	20.048146534461743
22	20.119882230231976	26.35736699189783	27.943474032118225	25.579276745751972
23	20.185896674192314	27.409637228625623	28.692517922921535	23.711948174260527
24	21.998213209050142	25.831892017973534	28.671833397147296	23.49806137582903
25	19.416168257614768	28.479951413369246	27.846652847643067	24.257227481372926
26	18.32737002856225	28.306113377607023	29.19642817848545	24.17008841534528
27	21.62765213028611	27.4241604062969	27.948315091341986	22.999872372075007
28	17.079256941418784	28.674914071198778	30.32967613313793	23.91615285424451
29	18.976512060838914	24.775660914608117	31.95187106938998	24.29595595516299
30	21.09161484532816	27.049198364602173	27.997605876165704	23.861580913903964
31	20.581103145368207	29.771193937233463	24.628668752723097	25.01903416467523
32	20.856163328536283	29.15417893435084	27.256483718637636	22.733174018475243
33	21.792248143893882	26.2741887925078	25.313458584738342	26.620104478859975
34	19.405165850288043	25.644410997126172	28.492274109575177	26.45814904301061
35	19.533673967864168	25.36318946585513	28.44650409509601	26.656632471184693
36	21.028240979126235	27.285970170273256	25.884703573141802	25.801085277458707
37	19.144188748498173	27.84445236617772	29.22107357089731	23.790285314426797
38	19.89763360223217	29.047675631428156	26.227978681735564	24.82671208460411
39	22.36657380634883	23.79336598847828	28.807383055412522	25.032677149760367
40	22.592343204693186	24.134000519313624	28.741368611452184	24.532287664541002
41	18.047908882463485	26.304995533022623	26.966900357798284	28.680195226715604
42	18.909617424292435	25.7091051522073	28.955695506176752	26.42558191732351
43	21.852541336044325	24.245784977753132	28.015649824181533	25.886023862021013
44	20.625992967261237	24.877763254600108	27.686457796965975	26.80978598117268
45	20.79234936604129	23.819771766062413	27.678536063690736	27.709342804205562
46	22.287796569889494	25.766317670306265	27.207633030106987	24.73825272969726
47	18.14737064469706	25.036197920104918	31.5522636352834	25.26416779991462
48	19.421009316838525	23.52358696082703	29.13217411969739	27.923229602637058
49	20.128244059800284	22.3344467769548	31.984438195077082	25.552870968167834
50	19.604529471048266	22.076990445509477	30.838867544218672	27.47961253922358
51	18.67944706301739	22.6728808263248	26.922450632198323	31.72522147845949
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	210.0
2	304.0
3	499.0
4	694.0
5	501.5
6	309.0
7	313.5
8	318.0
9	317.0
10	316.0
11	318.5
12	321.0
13	372.0
14	423.0
15	378.0
16	333.0
17	331.5
18	330.0
19	381.5
20	433.0
21	601.0
22	769.0
23	787.5
24	806.0
25	1158.0
26	1780.0
27	2050.0
28	2118.0
29	2186.0
30	3086.5
31	3987.0
32	4511.5
33	5036.0
34	5169.5
35	5303.0
36	5893.5
37	6484.0
38	7119.5
39	7755.0
40	8934.0
41	10113.0
42	11728.0
43	13343.0
44	15345.5
45	17348.0
46	21059.5
47	24771.0
48	26187.0
49	27603.0
50	26801.0
51	25999.0
52	21572.5
53	17146.0
54	14449.5
55	11753.0
56	10273.0
57	8793.0
58	8212.0
59	7631.0
60	7148.0
61	6665.0
62	6116.5
63	5568.0
64	4875.5
65	4183.0
66	3342.5
67	2502.0
68	2091.0
69	1680.0
70	1389.0
71	1098.0
72	846.0
73	594.0
74	445.0
75	226.0
76	156.0
77	138.5
78	121.0
79	81.5
80	42.0
81	31.5
82	21.0
83	13.5
84	6.0
85	5.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	227223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.16095201630117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80220529172651	25.96436100218728
2	11.324635493999951	8.190192014012666
3	4.10388725263491	4.45201410068523
4	2.099408514470706	3.036664422175572
5	1.3338850619477642	2.4117276860177004
6	1.018669522673612	2.210163583792134
7	0.7180585643696906	1.817597690374654
8	0.6426015626901639	1.8589667419231328
9	0.5793150451524962	1.8853725195072681
>10	6.236156074288635	40.43780779234496
>50	0.0888445342355719	2.2110437763782715
>100	0.0450307913248789	2.984733059593439
>500	0.004868193656743665	1.483124507642272
>1k	0.0024340968283718327	1.0562311033654164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1366	0.6011715363321495	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1034	0.4550595670332669	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	952	0.41897167100161514	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	849	0.37364175281551604	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	788	0.3467958789383117	No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	781	0.3437152048868292	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	437	0.19232208007111956	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	346	0.15227331740184752	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.14919264335036508	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	328	0.1443515841266069	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	282	0.12410715464543642	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	280	0.12322696205929856	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	276	0.12146657688702288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35559780479969016	0.0
2	0.0	0.0	0.0	1.8087957645132755	0.0
3	0.0	0.0	0.0	2.4786223225641772	0.0
4	0.0	0.0	0.0	3.411186367577226	0.0
5	0.0	0.0	0.0	5.990150644961117	0.0
6	0.0	0.0	0.0	7.132200525474974	0.0
7	0.0	0.0	0.0	8.378113131153096	0.0
8	0.0	0.0	0.0	10.24060064342078	0.0
9	0.0	0.0	0.0	10.881380846129133	0.0
10	0.0	0.0	0.0	12.674773240384996	0.0
11	0.0	0.0	0.0	14.862932009523684	0.0
12	0.0	0.0	0.0	16.956910171945623	0.0
13	0.0	0.0	0.0	17.699792714645966	0.0
14	0.0	0.0	0.0	18.02810454927538	0.0
15	0.0	0.0	0.0	18.62487512267684	0.0
16	0.0	0.0	0.0	19.74580038112339	0.0
17	0.0	0.0	0.0	21.036602808694543	0.0
18	0.0	0.0	0.0	22.381096984020104	0.0
19	0.0	0.0	0.0	23.315421414205428	0.0
20	0.0	0.0	0.0	24.111555608367112	0.0
21	0.0	0.0	0.0	25.071845719843502	0.0
22	0.0	0.0	0.0	26.10255123821092	0.0
23	0.0	0.0	0.0	27.03555537951704	0.0
24	0.0	0.0	0.0	27.78723984807876	0.0
25	0.0	0.0	0.0	28.456186213543525	0.0
26	0.0	0.0	0.0	29.081563045994464	0.0
27	0.0	0.0	0.0	29.660729767673168	0.0
28	0.0	0.0	0.0	30.279945252021143	0.0
29	0.0	0.0	0.0	30.915884395505735	0.0
30	0.0	0.0	0.0	31.597593553469498	0.0
31	0.0	0.0	0.0	32.19436412687096	0.0
32	0.0	0.0	0.0	32.79641585578925	0.0
33	0.0	0.0	0.0	33.35137728134916	0.0
34	0.0	0.0	0.0	33.90809909208135	0.0
35	0.0	0.0	0.0	34.505749858068945	0.0
36	0.0	0.0	0.0	35.09459869819516	0.0
37	0.0	0.0	0.0	35.6279954053947	0.0
38	0.0	0.0	0.0	36.165352979231855	0.0
39	0.0	0.0	0.0	36.72603565660167	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACAT	20	7.02133E-4	45.0	11
CTATGCC	25	3.880897E-5	45.0	9
TAGCGCG	25	3.880897E-5	45.0	1
TCCTCGA	20	7.02133E-4	45.0	38
GTCTACG	20	7.02133E-4	45.0	40
TATGCCC	25	3.880897E-5	45.0	10
CAGGGTC	20	7.02133E-4	45.0	5
AGACGAT	20	7.02133E-4	45.0	18
GTAGACG	25	3.880897E-5	45.0	1
CACTAGG	25	3.880897E-5	45.0	2
CTACGCA	20	7.02133E-4	45.0	42
CTACGAT	20	7.02133E-4	45.0	22
CGAGGGC	20	7.02133E-4	45.0	4
ACCAGGT	20	7.02133E-4	45.0	3
CGAATAT	25	3.880897E-5	45.0	14
GATTGCG	20	7.02133E-4	45.0	1
TGTAGCG	25	3.880897E-5	45.0	1
GTCCCGC	25	3.880897E-5	45.0	15
GCCCGAA	25	3.880897E-5	45.0	25
CCCGCGG	25	3.880897E-5	45.0	17
>>END_MODULE