##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935923.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853663
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13952344192029	33.0	31.0	34.0	30.0	34.0
2	32.264618473566266	34.0	31.0	34.0	30.0	34.0
3	32.28713555583409	34.0	31.0	34.0	30.0	34.0
4	35.84554209330848	37.0	35.0	37.0	35.0	37.0
5	35.89258407591755	37.0	35.0	37.0	35.0	37.0
6	35.92264511874124	37.0	35.0	37.0	35.0	37.0
7	36.106914555275324	37.0	35.0	37.0	35.0	37.0
8	35.89645328425854	37.0	35.0	37.0	35.0	37.0
9	37.63260912092945	39.0	38.0	39.0	35.0	39.0
10	37.272500975209184	39.0	37.0	39.0	34.0	39.0
11	37.19630931643986	39.0	37.0	39.0	34.0	39.0
12	37.248558271823896	39.0	37.0	39.0	34.0	39.0
13	37.33642198385077	39.0	37.0	39.0	34.0	39.0
14	38.59584871313387	40.0	38.0	41.0	34.0	41.0
15	38.715001118708436	40.0	38.0	41.0	35.0	41.0
16	38.775495716693825	40.0	38.0	41.0	35.0	41.0
17	38.74227300468686	40.0	38.0	41.0	35.0	41.0
18	38.65008908667706	40.0	38.0	41.0	35.0	41.0
19	38.60484875179081	40.0	38.0	41.0	35.0	41.0
20	38.422875303251985	40.0	37.0	41.0	34.0	41.0
21	38.414862773717495	40.0	37.0	41.0	34.0	41.0
22	38.404418371183944	40.0	37.0	41.0	34.0	41.0
23	38.43268948050929	40.0	37.0	41.0	34.0	41.0
24	38.37073294731059	40.0	37.0	41.0	34.0	41.0
25	38.29653270670042	40.0	37.0	41.0	34.0	41.0
26	38.163076061630875	40.0	37.0	41.0	34.0	41.0
27	38.117053216550325	40.0	37.0	41.0	34.0	41.0
28	38.09255760177025	40.0	37.0	41.0	34.0	41.0
29	38.04509976419266	40.0	37.0	41.0	34.0	41.0
30	37.90230571080157	40.0	37.0	41.0	34.0	41.0
31	37.893620784782755	40.0	37.0	41.0	34.0	41.0
32	37.734918814567344	40.0	37.0	41.0	33.0	41.0
33	37.56643195265579	40.0	37.0	41.0	33.0	41.0
34	37.42034151650007	40.0	37.0	41.0	33.0	41.0
35	37.31272762202415	40.0	36.0	41.0	32.0	41.0
36	37.210061815962504	40.0	36.0	41.0	32.0	41.0
37	37.180382656856395	40.0	36.0	41.0	32.0	41.0
38	37.0904279557624	40.0	36.0	41.0	31.0	41.0
39	37.05935831821222	40.0	36.0	41.0	31.0	41.0
40	37.006152310689345	40.0	36.0	41.0	31.0	41.0
41	36.90114248831213	40.0	35.0	41.0	31.0	41.0
42	36.884530546597425	40.0	35.0	41.0	31.0	41.0
43	36.83559788815961	40.0	35.0	41.0	31.0	41.0
44	36.77955703831606	40.0	35.0	41.0	31.0	41.0
45	36.69209043849857	40.0	35.0	41.0	31.0	41.0
46	36.63905663007533	39.0	35.0	41.0	31.0	41.0
47	36.62067818331121	39.0	35.0	41.0	31.0	41.0
48	36.55361073397816	39.0	35.0	41.0	31.0	41.0
49	36.503371939512434	39.0	35.0	41.0	31.0	41.0
50	36.385832582646785	39.0	35.0	41.0	30.0	41.0
51	35.45499922100407	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	21.0
11	13.0
12	20.0
13	21.0
14	28.0
15	37.0
16	47.0
17	75.0
18	186.0
19	262.0
20	464.0
21	769.0
22	1299.0
23	1897.0
24	3143.0
25	5705.0
26	8483.0
27	10059.0
28	10438.0
29	10580.0
30	11523.0
31	13750.0
32	17018.0
33	23276.0
34	40977.0
35	52718.0
36	56880.0
37	83732.0
38	166584.0
39	333302.0
40	338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.876699587542156	21.57959288384292	25.10756586615561	14.436141662459306
2	34.76078967929968	23.385457727463884	25.09362593904152	16.760126654194924
3	30.205479211351555	22.944651460822364	30.311492942765472	16.538376385060616
4	26.770517171295932	24.741144924870824	30.31371864541394	18.174619258419305
5	24.51517753492889	28.971034237163845	28.057793297823615	18.45599493008365
6	24.44992930465535	32.62013230045112	27.706483706099476	15.223454688794055
7	78.27433073707071	5.035476528794149	11.617933540518917	5.072259193616216
8	79.8682852601085	4.135706947589388	11.193175761395304	4.80283203090681
9	73.5920380759152	7.084997241300138	13.108217177035902	6.214747505748756
10	35.67742774373494	30.71797653172271	20.206802918716168	13.397792805826187
11	23.780812803178772	25.363053101750925	32.063238069355236	18.792896025715066
12	23.023605333720685	22.10790440724267	34.740992639952765	20.12749761908388
13	22.625438844134045	22.414114234774143	35.70050476593222	19.25994215515959
14	18.737839170726623	26.12682053690976	34.28484073926128	20.850499553102335
15	18.039319965841322	27.27258883189268	35.57117972783171	19.116911474434293
16	20.764165718790668	27.532293188295615	32.953987697721466	18.749553395192248
17	20.7030174670801	26.45329597276677	31.312707707842556	21.53097885231057
18	20.965884664088758	26.514327082232686	32.459178856293406	20.06060939738515
19	20.877559411617934	28.003556438547765	31.1816255360722	19.937258613762104
20	21.84175722738364	27.084926955953343	31.402555809493908	19.670760007169104
21	21.892011250341177	27.07133845557322	32.64684073223274	18.389809561852864
22	20.74811723127276	24.780036150096702	32.10564356192081	22.366203056709733
23	19.69758558119539	25.979924162110805	32.41150196271831	21.910988293975493
24	20.220391419096295	24.8753899372469	33.024273044515226	21.879945599141582
25	20.224491397659264	27.44841934112173	30.234296203536992	22.092793057682012
26	19.942881441505605	27.922962574224254	30.27201600631631	21.862139977953827
27	20.38146200549866	27.36032837314022	30.914189791521945	21.344019829839176
28	19.047211838863813	27.609021358545466	31.80177657928246	21.54199022330826
29	20.71520026052435	25.581171961300885	31.59537194419812	22.10825583397664
30	21.5941185221803	27.250448947652643	30.654134008385043	20.501298521782015
31	20.991304531179168	28.37454592737415	29.73374739212078	20.900402149325906
32	21.532618843735758	29.412777641762617	29.520079937867756	19.534523576633873
33	21.15635795389984	29.269161249814037	29.139601927224206	20.43487886906191
34	21.723326418036155	26.6072208822451	30.561825919595908	21.107626780122835
35	21.36615971407921	27.80277463120693	29.972014717751616	20.859050936962245
36	22.2216495268039	28.4633397488236	29.825001200708005	19.49000952366449
37	22.08564738075798	28.411680018930184	30.35823269838332	19.14443990192851
38	19.998992576695958	28.46181689964307	30.537460332707404	21.001730190953573
39	22.79810651275738	27.102849719385752	28.970799952674533	21.128243815182337
40	22.657418676925204	25.861141926029358	30.985412276272957	20.496027120772485
41	21.41817087070659	26.271725493549564	30.441520834333925	21.868582801409925
42	22.245077975735157	26.226040018133617	30.340192792706254	21.18868921342497
43	21.729886383736908	26.407259070616863	30.448197942279332	21.414656603366904
44	21.284862996287764	26.521472759156715	29.076696541843795	23.116967702711726
45	21.179552118341782	25.73474544404525	29.154830419029526	23.930872018583447
46	21.96967655854828	26.60944658489357	29.316135289921196	22.10474156663695
47	20.810085478695925	25.500929523711346	31.79861373867674	21.890371258915987
48	20.4879443058912	24.865081419717146	32.25265707896441	22.394317195427234
49	21.46327063489925	24.392646747018436	31.929461625957785	22.214620992124527
50	19.830073459901623	24.131536683679624	32.56542687219664	23.47296298422211
51	20.227419953775673	23.370932089126505	30.6579997024587	25.743648254639123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	307.0
1	541.5
2	776.0
3	6638.0
4	12500.0
5	8220.0
6	3940.0
7	3872.0
8	3804.0
9	3755.5
10	3707.0
11	3719.5
12	3732.0
13	3590.5
14	3449.0
15	3307.5
16	3166.0
17	3062.0
18	2958.0
19	2881.5
20	2805.0
21	3052.5
22	3300.0
23	3873.5
24	4447.0
25	5284.0
26	7037.5
27	7954.0
28	9862.5
29	11771.0
30	13562.0
31	15353.0
32	17667.0
33	19981.0
34	23418.5
35	26856.0
36	29206.0
37	31556.0
38	34979.5
39	38403.0
40	42165.5
41	45928.0
42	51707.0
43	57486.0
44	69479.0
45	81472.0
46	89532.5
47	97593.0
48	93681.0
49	89769.0
50	76598.5
51	63428.0
52	55905.5
53	48383.0
54	42837.0
55	37291.0
56	34415.5
57	31540.0
58	29134.5
59	26729.0
60	24507.0
61	22285.0
62	19811.0
63	17337.0
64	14357.0
65	11377.0
66	8898.0
67	6419.0
68	5123.0
69	3827.0
70	3100.0
71	2373.0
72	1984.5
73	1596.0
74	1252.0
75	749.0
76	590.0
77	416.5
78	243.0
79	160.5
80	78.0
81	73.0
82	68.0
83	49.5
84	31.0
85	17.0
86	3.0
87	1.5
88	0.0
89	10.5
90	21.0
91	10.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	853663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.653875559378044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9679528445781	26.7455826338773
2	10.560537332869076	7.741692424782985
3	3.7842764058506324	4.161251893870185
4	2.0476697076053325	3.0022012259709556
5	1.2448586779129345	2.281444753461629
6	1.0115557084951206	2.2246462236335183
7	0.8243199818315434	2.115016542461351
8	0.7108812248796842	2.0845241563390546
9	0.6305543447785625	2.080103443824473
>10	6.118040627176743	40.75407519150035
>50	0.06451505006214629	1.6115608687669651
>100	0.029999469798997147	1.9569276538318907
>500	0.0019354496644449746	0.49410915747372247
>1k	0.0025805995525932995	1.8480320965097587
>5k	3.2257494407416244E-4	0.8988317336959172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7602	0.8905153438769162	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	3080	0.36079811354129204	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	2198	0.2574786537544675	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2080	0.24365586888502838	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1920	0.2249131097400262	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	1805	0.21144175160455592	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1803	0.21120746711524338	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1375	0.16107058640236252	No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	1369	0.16036773293442494	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	863	0.10109375713835554	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.199957125938456E-4	0.0	0.0	0.22069598893240072	0.0
2	8.199957125938456E-4	0.0	0.0	0.8452984374395985	0.0
3	8.199957125938456E-4	0.0	0.0	1.5145320811608327	0.0
4	9.371379572501093E-4	0.0	0.0	2.1911456862954117	0.0
5	9.371379572501093E-4	0.0	0.0	3.8994310401177046	0.0
6	9.371379572501093E-4	0.0	0.0	5.328449282679465	0.0
7	9.371379572501093E-4	0.0	0.0	6.429234955714374	0.0
8	9.371379572501093E-4	0.0	0.0	7.921627152635174	0.0
9	9.371379572501093E-4	0.0	0.0	8.547049596854965	0.0
10	9.371379572501093E-4	0.0	0.0	9.7567775574202	0.0
11	9.371379572501093E-4	0.0	0.0	11.384820473652953	0.0
12	9.371379572501093E-4	0.0	0.0	12.697047898292418	0.0
13	9.371379572501093E-4	0.0	0.0	13.297284759911113	0.0
14	0.0010542802019063728	0.0	0.0	13.58768038441399	0.0
15	0.0010542802019063728	0.0	0.0	13.966401261387691	0.0
16	0.0010542802019063728	0.0	0.0	14.705334540679402	0.0
17	0.0010542802019063728	0.0	0.0	15.694600796801549	0.0
18	0.0010542802019063728	0.0	0.0	16.735292498327794	0.0
19	0.0010542802019063728	0.0	0.0	17.407454698165434	0.0
20	0.0010542802019063728	0.0	0.0	18.035454271767666	0.0
21	0.0010542802019063728	0.0	0.0	18.78563320654638	0.0
22	0.0010542802019063728	0.0	0.0	19.657640075767603	0.0
23	0.0010542802019063728	0.0	0.0	20.486655741199982	0.0
24	0.0010542802019063728	0.0	0.0	21.14159803107315	0.0
25	0.0011714224465626366	0.0	0.0	21.72801210782241	0.0
26	0.0011714224465626366	0.0	0.0	22.273074972208004	0.0
27	0.0011714224465626366	0.0	0.0	22.832663474930975	0.0
28	0.0012885646912189001	0.0	0.0	23.396703382950882	0.0
29	0.0012885646912189001	0.0	0.0	24.001743076600484	0.0
30	0.0012885646912189001	0.0	0.0	24.686205212127035	0.0
31	0.0014057069358751639	0.0	0.0	25.305419117380044	0.0
32	0.0014057069358751639	0.0	0.0	25.90600740573271	0.0
33	0.0014057069358751639	0.0	0.0	26.487970077185025	0.0
34	0.0014057069358751639	0.0	0.0	27.049198571333186	0.0
35	0.0014057069358751639	0.0	0.0	27.658103959056444	0.0
36	0.0014057069358751639	0.0	0.0	28.23538094072251	0.0
37	0.0014057069358751639	0.0	0.0	28.810197935250795	0.0
38	0.0014057069358751639	0.0	0.0	29.386186352225643	0.0
39	0.0014057069358751639	0.0	0.0	29.959597639818053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACGC	20	7.032264E-4	45.000004	36
GCATCCG	20	7.032264E-4	45.000004	1
TTGCGCG	90	0.0	45.0	1
TCGCGCG	25	3.8899598E-5	45.0	1
CGTTTTT	3995	0.0	42.972466	1
TATAGCG	45	1.9272193E-8	40.0	1
ACGGGCC	85	0.0	39.705883	5
GCGATAT	40	3.4568257E-7	39.375004	9
GTACGAG	40	3.4568257E-7	39.375004	1
AGGCGTC	35	6.2464005E-6	38.571426	35
GCTACGA	140	0.0	38.571426	10
TTAGCGG	200	0.0	38.25	2
CATACGA	165	0.0	38.181816	18
TAGGGCG	130	0.0	38.076927	5
TCTCGGA	30	1.1396757E-4	37.500004	36
TTACGAG	30	1.1396757E-4	37.500004	1
GATTCGA	30	1.1396757E-4	37.500004	9
AATGCGG	115	0.0	37.173912	2
TACGGGA	370	0.0	37.094597	4
CTACGAA	140	0.0	36.964283	11
>>END_MODULE