##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935918.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672357
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11196432847431	33.0	31.0	34.0	30.0	34.0
2	32.23756278286684	33.0	31.0	34.0	30.0	34.0
3	32.27959997441835	34.0	31.0	34.0	30.0	34.0
4	35.84479971205773	37.0	35.0	37.0	35.0	37.0
5	35.88623603234591	37.0	35.0	37.0	35.0	37.0
6	35.91155442718675	37.0	35.0	37.0	35.0	37.0
7	36.08936324006443	37.0	35.0	37.0	35.0	37.0
8	35.822223907834676	37.0	35.0	37.0	35.0	37.0
9	37.60026146823785	39.0	38.0	39.0	35.0	39.0
10	37.12531437911704	39.0	37.0	39.0	33.0	39.0
11	37.115722748480344	39.0	37.0	39.0	34.0	39.0
12	37.18343826270865	39.0	37.0	39.0	34.0	39.0
13	37.26729847387623	39.0	37.0	39.0	34.0	39.0
14	38.50122182114561	40.0	38.0	41.0	34.0	41.0
15	38.621846727259474	40.0	38.0	41.0	35.0	41.0
16	38.70363512241265	40.0	38.0	41.0	35.0	41.0
17	38.68309841349164	40.0	38.0	41.0	35.0	41.0
18	38.654955626252125	40.0	38.0	41.0	35.0	41.0
19	38.623046982481036	40.0	38.0	41.0	35.0	41.0
20	38.46972069897391	40.0	38.0	41.0	34.0	41.0
21	38.493548814097274	40.0	38.0	41.0	34.0	41.0
22	38.50228970621262	40.0	38.0	41.0	34.0	41.0
23	38.50650919080191	40.0	38.0	41.0	34.0	41.0
24	38.436045136735395	40.0	38.0	41.0	34.0	41.0
25	38.39667765785141	40.0	38.0	41.0	34.0	41.0
26	38.25840290202972	40.0	37.0	41.0	34.0	41.0
27	38.20094235651596	40.0	37.0	41.0	34.0	41.0
28	38.22216917500673	40.0	37.0	41.0	34.0	41.0
29	38.19777737124772	40.0	37.0	41.0	34.0	41.0
30	38.10561651027653	40.0	37.0	41.0	34.0	41.0
31	38.1398364261843	40.0	37.0	41.0	34.0	41.0
32	38.01981239133377	40.0	37.0	41.0	34.0	41.0
33	38.00715839948123	40.0	37.0	41.0	34.0	41.0
34	37.94622945845734	40.0	37.0	41.0	34.0	41.0
35	37.91349982226704	40.0	37.0	41.0	34.0	41.0
36	37.86022752793531	40.0	37.0	41.0	33.0	41.0
37	37.795630892516925	40.0	37.0	41.0	33.0	41.0
38	37.71025214283483	40.0	37.0	41.0	33.0	41.0
39	37.66465731746676	40.0	37.0	41.0	33.0	41.0
40	37.650472888658854	40.0	37.0	41.0	33.0	41.0
41	37.60454936886208	40.0	37.0	41.0	33.0	41.0
42	37.60333275328434	40.0	37.0	41.0	33.0	41.0
43	37.51395910208416	40.0	36.0	41.0	33.0	41.0
44	37.449966312539324	40.0	36.0	41.0	33.0	41.0
45	37.35782776114475	40.0	36.0	41.0	33.0	41.0
46	37.29652996845426	40.0	36.0	41.0	32.0	41.0
47	37.25914506727825	40.0	36.0	41.0	32.0	41.0
48	37.218714165242574	39.0	36.0	41.0	32.0	41.0
49	37.17799175140588	39.0	36.0	41.0	32.0	41.0
50	37.05739361678394	39.0	35.0	41.0	32.0	41.0
51	36.062872848799074	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	6.0
9	9.0
10	12.0
11	8.0
12	10.0
13	10.0
14	7.0
15	8.0
16	31.0
17	56.0
18	106.0
19	176.0
20	348.0
21	692.0
22	1039.0
23	1525.0
24	2097.0
25	2925.0
26	3853.0
27	4513.0
28	5065.0
29	5962.0
30	7366.0
31	9924.0
32	12987.0
33	18204.0
34	32258.0
35	43141.0
36	44737.0
37	69449.0
38	138515.0
39	267071.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.861625594736125	22.565393087303324	26.21419870693694	10.358782611023608
2	31.38020426648343	25.587448334738838	25.5739138582628	17.458433540514935
3	30.59133763759431	25.44957515129611	27.45163655617477	16.507450654934804
4	27.701652544704675	25.62834922518841	28.060390536575063	18.60960769353186
5	26.320243561084368	29.685568827274793	25.202087581448545	18.792100030192294
6	25.352900319324405	34.62505781898604	25.18022419637187	14.84181766531768
7	80.36251574684282	5.590036245625464	9.354554202603676	4.692893804928037
8	81.4196922170811	5.563413484205563	8.000214171935445	5.016680126777887
9	75.95548198352958	6.959844249409168	9.914227114464488	7.17044665259676
10	37.54850473781042	28.022910447872185	19.18950795485137	15.239076859466028
11	25.874646950950165	25.723834213074305	27.41028947419303	20.991229361782505
12	23.24181945008381	22.48359130640419	32.028371832226036	22.24621741128597
13	23.579288978920424	24.172277525183794	33.09581070770439	19.152622788191394
14	19.877981489000636	28.324982115156082	30.839271398974056	20.957764996869223
15	19.509278552911624	26.67704805631532	34.023889094632764	19.789784296140294
16	22.104774695585828	26.32455674589541	30.250149845989554	21.320518712529207
17	22.1462705080783	26.625141108072054	28.831260773666372	22.397327610183282
18	22.15281465055023	25.929231048386498	30.949629437932526	20.968324863130746
19	21.258200628535135	28.663641488078508	28.768199037118674	21.309958846267683
20	22.294406096761097	27.825693790649908	30.278259912516713	19.601640200072282
21	21.671820178863314	28.534394674257875	30.54508244875862	19.248702698120194
22	20.868675420944527	24.919202150048264	29.910746820513506	24.301375608493704
23	19.89196215700885	27.103755891587355	29.981840004640393	23.0224419467634
24	20.471862418328357	26.51210591992052	30.42609804017806	22.589933621573064
25	19.74174434117589	29.643626823250145	28.034362697198066	22.580266138375894
26	19.928996054179553	27.88087281012914	30.03969617331269	22.15043496237862
27	21.619169578066412	27.921922431089435	29.3100242876924	21.14888370315175
28	19.430005190694825	26.705604314374654	31.021168813591586	22.84322168133893
29	20.232108835038527	24.267465052048244	32.39410015810053	23.106325954812696
30	23.347120651677606	26.55226315781646	29.332780055833435	20.7678361346725
31	23.613794457408787	26.218363161237257	27.737645328300292	22.43019705305366
32	22.552007341338008	26.22862556647733	29.478833417366072	21.740533674818586
33	23.93891935385517	24.925895023030918	28.296277126586023	22.838908496527885
34	22.840395801635143	24.264192980812275	29.80172735615157	23.09368386140101
35	22.024906411326125	23.71954185053476	30.257437641015116	23.998114097124
36	24.66992981407199	24.868187584869347	29.543233728510298	20.918648872548363
37	23.350392722913572	24.77225640545127	30.843882044806552	21.033468826828607
38	22.185981554442062	25.26485185697479	29.093323933565056	23.455842655018095
39	23.435020383516495	24.150860331639294	30.703182981660042	21.710936303184173
40	23.70199165026913	23.247471209491387	29.758595508041115	23.29194163219837
41	21.4518477535	24.249022468718255	29.611203571911947	24.687926205869797
42	22.214537812501394	23.574380872066477	31.018342933887798	23.192738381544327
43	22.884568763320676	23.462684258511477	31.552880389435966	22.099866588731878
44	22.453250282216146	23.72474741841016	29.38319969896945	24.438802600404248
45	23.651274546111665	22.559443866874297	29.04721747524009	24.742064111773953
46	25.244327046494647	23.901587995663018	28.76909142018303	22.084993537659308
47	21.541680981978324	23.592972185907186	32.177845995505365	22.68750083660912
48	21.87915051081494	23.324364883536575	30.821721198708424	23.974763406940063
49	23.240927067019456	21.864574920763822	32.26515080530135	22.629347206915373
50	22.353303379008473	21.733989532346655	31.48372070194852	24.42898638669635
51	21.854758707055925	21.373615504858282	29.014794223901884	27.75683156418391
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	326.0
2	497.0
3	1466.0
4	2435.0
5	1730.5
6	1026.0
7	1032.0
8	1038.0
9	1092.5
10	1147.0
11	1185.5
12	1224.0
13	1191.0
14	1158.0
15	1154.5
16	1151.0
17	1140.0
18	1129.0
19	1395.5
20	1662.0
21	1753.5
22	1845.0
23	2244.0
24	2643.0
25	3365.5
26	4844.5
27	5601.0
28	6524.5
29	7448.0
30	8848.5
31	10249.0
32	12171.5
33	14094.0
34	16752.5
35	19411.0
36	21727.0
37	24043.0
38	25570.5
39	27098.0
40	29348.5
41	31599.0
42	34835.5
43	38072.0
44	42109.0
45	46146.0
46	49914.5
47	53683.0
48	64293.0
49	74903.0
50	78022.0
51	81141.0
52	77507.5
53	73874.0
54	61726.0
55	49578.0
56	40914.5
57	32251.0
58	26665.5
59	21080.0
60	17864.5
61	14649.0
62	12404.0
63	10159.0
64	8232.0
65	6305.0
66	5157.0
67	4009.0
68	3313.5
69	2618.0
70	1949.5
71	1281.0
72	1070.0
73	859.0
74	761.0
75	472.5
76	282.0
77	163.0
78	44.0
79	25.0
80	6.0
81	6.5
82	7.0
83	4.5
84	2.0
85	1.0
86	0.0
87	1.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.250895986794326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81547471388038	26.751907788090158
2	11.566494684453275	8.617245808447569
3	4.378883269251956	4.8935197560365555
4	2.2052758443926344	3.285940044066402
5	1.4332265794385108	2.6694487118086494
6	1.026865076653834	2.2950986497742134
7	0.7748532511169608	2.020478450367113
8	0.6284863948088593	1.8729345057712135
9	0.5618472922652601	1.883638354017171
>10	5.506250257195307	39.450621630613234
>50	0.06406487890994512	1.6319580691295794
>100	0.0338455962229952	2.3202832416427785
>500	0.0016116950582378664	0.4075017142803638
>1k	0.0028204663519162663	1.8994232759550662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	2226	0.33107411687540994	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	2138	0.3179858319315483	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2029	0.3017742062624469	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	1843	0.27411033126746653	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	1753	0.2607245853021535	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	1375	0.2045044522478386	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1291	0.19201108934687972	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	901	0.1340061901638564	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	716	0.10649104567960176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.949220429028032E-4	0.0	0.0	0.2116435167626722	0.0
2	5.949220429028032E-4	0.0	0.0	0.9984279185016294	0.0
3	5.949220429028032E-4	0.0	0.0	1.4646980696267013	0.0
4	5.949220429028032E-4	0.0	0.0	2.113609287922934	0.0
5	5.949220429028032E-4	0.0	0.0	4.0140877539759385	0.0
6	5.949220429028032E-4	0.0	0.0	4.991842131486695	0.0
7	5.949220429028032E-4	0.0	0.0	5.975843190447931	0.0
8	5.949220429028032E-4	0.0	0.0	7.5437602345182695	0.0
9	5.949220429028032E-4	0.0	0.0	8.057921610097017	0.0
10	5.949220429028032E-4	0.0	0.0	9.558612463319339	0.0
11	5.949220429028032E-4	0.0	0.0	11.43112959335591	0.0
12	5.949220429028032E-4	0.0	0.0	13.232999730797776	0.0
13	5.949220429028032E-4	0.0	0.0	13.850082619798709	0.0
14	5.949220429028032E-4	0.0	0.0	14.099206225264258	0.0
15	5.949220429028032E-4	0.0	0.0	14.551495708381113	0.0
16	5.949220429028032E-4	0.0	0.0	15.482102513991823	0.0
17	5.949220429028032E-4	0.0	0.0	16.76386205542591	0.0
18	5.949220429028032E-4	0.0	0.0	18.02836885761582	0.0
19	5.949220429028032E-4	0.0	0.0	18.968494415912975	0.0
20	5.949220429028032E-4	0.0	0.0	19.732969241043076	0.0
21	5.949220429028032E-4	0.0	0.0	20.70670789476424	0.0
22	5.949220429028032E-4	0.0	0.0	21.740979866350763	0.0
23	5.949220429028032E-4	0.0	0.0	22.814516692768873	0.0
24	5.949220429028032E-4	0.0	0.0	23.619148755794914	0.0
25	5.949220429028032E-4	0.0	0.0	24.38406977245719	0.0
26	5.949220429028032E-4	0.0	0.0	25.054398184297924	0.0
27	5.949220429028032E-4	0.0	0.0	25.746738711726064	0.0
28	5.949220429028032E-4	0.0	0.0	26.44844926132992	0.0
29	5.949220429028032E-4	0.0	0.0	27.188234821679554	0.0
30	5.949220429028032E-4	0.0	0.0	27.996882608495188	0.0
31	5.949220429028032E-4	0.0	0.0	28.71539970581105	0.0
32	5.949220429028032E-4	0.0	0.0	29.417853907968535	0.0
33	5.949220429028032E-4	0.0	0.0	30.092495504620313	0.0
34	5.949220429028032E-4	0.0	0.0	30.74051433985219	0.0
35	5.949220429028032E-4	0.0	0.0	31.426459455319122	0.0
36	5.949220429028032E-4	0.0	0.0	32.06510826837528	0.0
37	5.949220429028032E-4	0.0	0.0	32.74659146852044	0.0
38	5.949220429028032E-4	0.0	0.0	33.409037163292716	0.0
39	5.949220429028032E-4	0.0	0.0	34.08561225658393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	35	1.2109194E-7	45.000004	1
TAGCACG	30	2.1641517E-6	45.000004	1
TTGCGCG	60	0.0	45.000004	1
CTCGCGG	30	2.1641517E-6	45.000004	2
GCGCGAC	40	6.8084773E-9	45.0	9
GTTTACG	25	3.8890725E-5	45.0	1
TCGATAG	45	3.8380676E-10	45.0	1
AGCGAAC	20	7.031196E-4	45.0	31
CTCGACT	20	7.031196E-4	45.0	29
CGCACGG	20	7.031196E-4	45.0	2
TCGACGG	50	2.1827873E-11	45.0	2
TAACGTC	120	0.0	41.250004	32
CGTTTTT	995	0.0	40.92965	1
TACGGTT	55	6.002665E-11	40.90909	33
CTCGTAC	50	1.0804797E-9	40.5	29
CCTCCGC	50	1.0804797E-9	40.5	45
AAACCGG	50	1.0804797E-9	40.5	2
TACGAAG	45	1.9263098E-8	40.0	1
GGCGCGA	45	1.9263098E-8	40.0	8
TAACGCG	40	3.4553705E-7	39.375	1
>>END_MODULE