##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935917.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	783937
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.105981730674785	33.0	31.0	34.0	30.0	34.0
2	32.23142931128394	33.0	31.0	34.0	30.0	34.0
3	32.28686999082834	34.0	31.0	34.0	30.0	34.0
4	35.846273361252244	37.0	35.0	37.0	35.0	37.0
5	35.84930804388618	37.0	35.0	37.0	35.0	37.0
6	35.861934058476635	37.0	35.0	37.0	35.0	37.0
7	35.992556799844884	37.0	35.0	37.0	35.0	37.0
8	35.578177838270165	37.0	35.0	37.0	33.0	37.0
9	37.35761674726413	39.0	37.0	39.0	34.0	39.0
10	37.17383922432543	39.0	37.0	39.0	34.0	39.0
11	37.1037187937296	39.0	37.0	39.0	34.0	39.0
12	36.97971648231937	39.0	37.0	39.0	33.0	39.0
13	36.92646985663389	39.0	37.0	39.0	33.0	39.0
14	38.04668487391206	40.0	37.0	41.0	33.0	41.0
15	38.157396576510614	40.0	37.0	41.0	33.0	41.0
16	38.328516194541145	40.0	37.0	41.0	34.0	41.0
17	38.252777965576314	40.0	37.0	41.0	34.0	41.0
18	38.2384528348579	40.0	37.0	41.0	34.0	41.0
19	38.25048058708799	40.0	37.0	41.0	34.0	41.0
20	37.993212464777145	40.0	37.0	41.0	33.0	41.0
21	38.05319432556443	40.0	37.0	41.0	33.0	41.0
22	38.01230200896246	40.0	37.0	41.0	34.0	41.0
23	37.96412466818124	40.0	36.0	41.0	34.0	41.0
24	37.9206479602315	40.0	36.0	41.0	33.0	41.0
25	37.906606015534415	40.0	36.0	41.0	33.0	41.0
26	37.69884569805992	40.0	36.0	41.0	33.0	41.0
27	37.61994905202842	40.0	36.0	41.0	33.0	41.0
28	37.572970787193356	40.0	36.0	41.0	33.0	41.0
29	37.541620053652274	40.0	36.0	41.0	33.0	41.0
30	37.487549382156985	40.0	36.0	41.0	33.0	41.0
31	37.526972192918564	40.0	36.0	41.0	33.0	41.0
32	37.36649246049109	40.0	35.0	41.0	33.0	41.0
33	37.33488405318285	40.0	35.0	41.0	32.0	41.0
34	37.179159804933306	40.0	35.0	41.0	32.0	41.0
35	37.13306171286723	40.0	35.0	41.0	32.0	41.0
36	37.1002044807172	40.0	35.0	41.0	32.0	41.0
37	37.05077703948149	40.0	35.0	41.0	32.0	41.0
38	36.96221507595636	40.0	35.0	41.0	31.0	41.0
39	36.839555984728364	39.0	35.0	41.0	31.0	41.0
40	36.771797988869004	39.0	35.0	41.0	31.0	41.0
41	36.68575536044351	39.0	35.0	41.0	31.0	41.0
42	36.647257368895716	39.0	35.0	41.0	31.0	41.0
43	36.568313525193986	39.0	35.0	41.0	31.0	41.0
44	36.56175942709682	39.0	35.0	41.0	31.0	41.0
45	36.47513384366346	39.0	35.0	41.0	31.0	41.0
46	36.428110932383596	39.0	35.0	41.0	31.0	41.0
47	36.31513374161444	39.0	35.0	41.0	31.0	41.0
48	36.26725872104519	39.0	35.0	41.0	31.0	41.0
49	36.2565997012515	39.0	35.0	41.0	31.0	41.0
50	36.1148663731907	38.0	35.0	41.0	30.0	41.0
51	35.16581434477516	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	8.0
9	21.0
10	25.0
11	29.0
12	23.0
13	24.0
14	27.0
15	38.0
16	51.0
17	107.0
18	191.0
19	439.0
20	891.0
21	1648.0
22	2717.0
23	3687.0
24	4734.0
25	5327.0
26	6172.0
27	6928.0
28	7610.0
29	8820.0
30	10811.0
31	14060.0
32	18367.0
33	26511.0
34	50141.0
35	57925.0
36	55937.0
37	83634.0
38	145057.0
39	271695.0
40	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.54996128515429	20.370514467361538	22.921229639626652	11.158294607857519
2	30.738311879653597	29.081801216169158	22.540586807358245	17.639300096819007
3	31.337594730188776	28.751927769705983	23.654069140760036	16.256408359345205
4	28.131724870748542	23.86237669608655	28.789941028424476	19.215957404740433
5	29.327994468943295	27.633470546740362	22.96000826597035	20.07852671834599
6	25.521821268800938	36.53048650593096	23.374072151206025	14.573620074062074
7	76.15611968818922	6.207896808034318	12.659435643425429	4.976547860351023
8	75.8221642810583	9.590311466355077	9.093332755055572	5.494191497531052
9	69.87997760024084	7.754194533489299	10.426603158161944	11.939224708107922
10	43.76652715715676	23.70636926181568	17.319759113296094	15.207344467731462
11	33.10368052534834	24.093900402710933	23.385297543042363	19.417121528898367
12	27.87213768453332	21.215735448129124	27.91499827154478	22.997128595792773
13	27.33484961163971	21.923190256359888	30.705911316853268	20.036048815147137
14	21.31752934228133	28.268087869305823	28.56785685584428	21.84652593256856
15	19.575169943503113	24.556182448334496	33.66023035014293	22.20841725801946
16	23.43402594851372	25.26631604325348	27.81639340916426	23.483264599068548
17	24.089308196959706	26.26410030397851	27.12756254648014	22.51902895258165
18	24.045682242323043	24.730813828152005	27.900328725395024	23.323175204129925
19	24.288686463325497	26.033724648791928	26.03168366845805	23.64590521942452
20	26.425082627813207	26.767967323904855	27.618418316778005	19.188531731503936
21	24.82138233046788	28.31630602969371	27.437281312146254	19.42503032769215
22	23.37598557026904	22.863699506465444	29.82433537388846	23.935979549377056
23	24.137526357347593	25.85003641874283	28.251249781551323	21.761187442358253
24	23.94069931639915	25.500135852753473	27.12603181122973	23.433133019617646
25	22.649013887595558	29.029628656384375	25.43546228842369	22.885895167596377
26	21.605690253170852	25.92147073042859	27.729013938620067	24.743825077780485
27	24.120178024509624	26.114726055792747	26.523559928922857	23.24153599077477
28	19.35397869981899	27.969849618017772	28.876172447530863	23.799999234632374
29	22.043225412246137	26.5469036414916	28.25010173011352	23.159769216148746
30	22.7283569980751	26.964921926124163	27.490474362097977	22.81624671370276
31	24.191867458737118	24.674304185157737	26.67829175048505	24.455536605620097
32	25.148194306430238	26.444216818443316	27.118378134977682	21.28921074014876
33	24.736043840257572	24.032798553965435	27.24249525153169	23.988662354245303
34	23.658151101427794	25.225368875305033	27.9074721565636	23.209007866703573
35	22.84061091643844	25.41518004635577	26.523687490193726	25.22052154701207
36	23.702287301147923	28.119478988745268	26.48886326324692	21.689370446859886
37	22.839080181188027	25.192075383608632	28.487110571385198	23.48173386381814
38	22.14195783589753	24.877636850920418	27.798662392513684	25.18174292066837
39	24.64088313219047	24.08841526806363	28.456623427647887	22.81407817209801
40	23.357744308534997	22.99432224783369	29.312942238980938	24.334991204650375
41	22.990112725895067	24.648919492255118	26.32890142957916	26.032066352270654
42	22.203697490997364	23.6538140182183	28.679855651665886	25.46263283911845
43	24.065071555494892	23.530972514372966	28.08860916119535	24.31534676893679
44	22.80923084380505	23.197017107242036	28.55803463798749	25.43571741096542
45	21.726745899224046	22.819945990557915	28.004163599881117	27.449144510336925
46	24.335629011004713	24.942693099062808	27.04464772041631	23.677030169516172
47	21.219944970067747	24.459873688829585	31.213094929822166	23.1070864112805
48	21.905969484792784	24.430024351446608	28.467211013129884	25.196795150630724
49	22.49504743365857	22.565461255177393	31.24613329897683	23.693358012187204
50	21.89716775710293	22.90566716458083	29.86821645106686	25.328948627249382
51	21.571758955120117	22.31709946079851	27.325537638866386	28.785603945214987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	668.0
2	1058.0
3	2086.5
4	3115.0
5	2171.5
6	1228.0
7	1213.0
8	1198.0
9	1204.5
10	1211.0
11	1230.0
12	1249.0
13	1219.0
14	1189.0
15	1292.5
16	1396.0
17	1444.0
18	1492.0
19	1497.5
20	1503.0
21	1743.5
22	1984.0
23	2059.0
24	2134.0
25	3025.5
26	4644.0
27	5371.0
28	6586.0
29	7801.0
30	9756.0
31	11711.0
32	12888.0
33	14065.0
34	16104.5
35	18144.0
36	19872.0
37	21600.0
38	23481.0
39	25362.0
40	28410.0
41	31458.0
42	34907.0
43	38356.0
44	42277.0
45	46198.0
46	65786.5
47	85375.0
48	79208.0
49	73041.0
50	72119.5
51	71198.0
52	64115.5
53	57033.0
54	52144.5
55	47256.0
56	45202.5
57	43149.0
58	40899.5
59	38650.0
60	36277.0
61	33904.0
62	31138.0
63	28372.0
64	24432.0
65	20492.0
66	17654.0
67	14816.0
68	12570.0
69	10324.0
70	8528.5
71	6733.0
72	5647.0
73	4561.0
74	3720.0
75	2218.0
76	1557.0
77	1201.0
78	845.0
79	639.0
80	433.0
81	317.0
82	201.0
83	121.0
84	41.0
85	40.5
86	40.0
87	25.0
88	10.0
89	6.0
90	2.0
91	2.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	783937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.44884585167059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40158528064602	21.026942784946563
2	10.353897036748586	6.09820635598556
3	3.788242337850122	3.346780939683615
4	1.9015714392356151	2.239963367599561
5	1.2166538475885051	1.7914525806237906
6	0.9228722346467495	1.6306513307339339
7	0.730397907284501	1.505656276740284
8	0.6232545926391063	1.4683302739979833
9	0.5826667343164291	1.5442976557602743
>10	8.341656165376815	50.695469094593214
>50	0.07934069870902996	1.5305958955406855
>100	0.05128652894052657	2.7593841471578497
>500	0.0017533856048043274	0.3471175136464941
>1k	0.0035067712096086548	1.3842101520384367
>5k	0.0013150392036032454	2.6309416309517277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	7406	0.9447187720441822	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	6973	0.8894847417585852	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	6002	0.7656227477463112	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2134	0.2722157520310943	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1581	0.2016743692414059	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1338	0.17067698042062054	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	1292	0.1648091619607188	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	1263	0.16110988510556334	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	1064	0.13572519220294488	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1044	0.1331739667855963	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	1007	0.1284541997635014	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	816	0.1040899970278224	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.551225417348588E-4	0.0	0.0	0.10855464150818242	0.0
2	2.551225417348588E-4	0.0	0.0	0.6235194919999949	0.0
3	2.551225417348588E-4	0.0	0.0	0.8990518370736424	0.0
4	2.551225417348588E-4	0.0	0.0	1.4341713683625088	0.0
5	2.551225417348588E-4	0.0	0.0	3.3937676114279594	0.0
6	2.551225417348588E-4	0.0	0.0	4.1124478114950564	0.0
7	2.551225417348588E-4	0.0	0.0	4.87845324305397	0.0
8	2.551225417348588E-4	0.0	0.0	5.942824487171801	0.0
9	2.551225417348588E-4	0.0	0.0	6.307649721852649	0.0
10	2.551225417348588E-4	0.0	0.0	7.851651344432014	0.0
11	2.551225417348588E-4	0.0	0.0	8.972021986460646	0.0
12	2.551225417348588E-4	0.0	0.0	10.803674274846065	0.0
13	2.551225417348588E-4	0.0	0.0	11.225136713792052	0.0
14	3.826838126022882E-4	0.0	0.0	11.424387418886976	0.0
15	3.826838126022882E-4	0.0	0.0	11.863708435754404	0.0
16	3.826838126022882E-4	0.0	0.0	12.46924178856209	0.0
17	3.826838126022882E-4	0.0	0.0	13.137790409178288	0.0
18	3.826838126022882E-4	0.0	0.0	13.831085916342767	0.0
19	3.826838126022882E-4	0.0	0.0	14.496062821374677	0.0
20	3.826838126022882E-4	0.0	0.0	14.996868370800204	0.0
21	3.826838126022882E-4	0.0	0.0	15.572042141141443	0.0
22	3.826838126022882E-4	0.0	0.0	16.218012416814105	0.0
23	3.826838126022882E-4	0.0	0.0	16.755555612249452	0.0
24	3.826838126022882E-4	0.0	0.0	17.210439104162706	0.0
25	3.826838126022882E-4	0.0	0.0	17.64606084417498	0.0
26	3.826838126022882E-4	0.0	0.0	18.060507413223256	0.0
27	3.826838126022882E-4	0.0	0.0	18.52738166459805	0.0
28	3.826838126022882E-4	0.0	0.0	18.94259360127153	0.0
29	5.102450834697176E-4	0.0	0.0	19.361249692258433	0.0
30	6.37806354337147E-4	0.0	0.0	19.90134411311113	0.0
31	6.37806354337147E-4	0.0	0.0	20.35788590154566	0.0
32	6.37806354337147E-4	0.0	0.0	20.79554862189181	0.0
33	6.37806354337147E-4	0.0	0.0	21.232445974612755	0.0
34	6.37806354337147E-4	0.0	0.0	21.695876071674128	0.0
35	7.653676252045763E-4	0.0	0.0	22.15471396298427	0.0
36	8.929288960720057E-4	0.0	0.0	22.59645864399818	0.0
37	8.929288960720057E-4	0.0	0.0	23.061291915039092	0.0
38	8.929288960720057E-4	0.0	0.0	23.55992892285987	0.0
39	8.929288960720057E-4	0.0	0.0	24.204240902011257	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	20	7.031911E-4	45.000004	1
GCGATCG	30	2.1646138E-6	45.000004	33
CCGCGTA	20	7.031911E-4	45.000004	40
CGGTCGT	20	7.031911E-4	45.000004	29
CGATCGC	30	2.1646138E-6	45.000004	34
TTCGAAT	20	7.031911E-4	45.000004	16
CTACGCG	25	3.8896673E-5	45.0	1
ATAACGG	45	3.8380676E-10	45.0	2
GCGTAAC	25	3.8896673E-5	45.0	32
CCGTCGA	100	0.0	42.75	41
CCGATGA	950	0.0	42.39474	18
CGAATAT	60	3.6379788E-12	41.250004	14
TACGGGA	115	0.0	41.08696	4
CGATGAA	1030	0.0	40.631065	19
TACGGCT	805	0.0	40.52795	7
GCGATAT	50	1.0804797E-9	40.5	9
CGTAAGC	50	1.0804797E-9	40.5	43
CACCCGT	90	0.0	40.0	38
CGTTTTT	930	0.0	39.67742	1
GATGAAT	1095	0.0	39.65753	20
>>END_MODULE