##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935917.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 783937 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.105981730674785 33.0 31.0 34.0 30.0 34.0 2 32.23142931128394 33.0 31.0 34.0 30.0 34.0 3 32.28686999082834 34.0 31.0 34.0 30.0 34.0 4 35.846273361252244 37.0 35.0 37.0 35.0 37.0 5 35.84930804388618 37.0 35.0 37.0 35.0 37.0 6 35.861934058476635 37.0 35.0 37.0 35.0 37.0 7 35.992556799844884 37.0 35.0 37.0 35.0 37.0 8 35.578177838270165 37.0 35.0 37.0 33.0 37.0 9 37.35761674726413 39.0 37.0 39.0 34.0 39.0 10 37.17383922432543 39.0 37.0 39.0 34.0 39.0 11 37.1037187937296 39.0 37.0 39.0 34.0 39.0 12 36.97971648231937 39.0 37.0 39.0 33.0 39.0 13 36.92646985663389 39.0 37.0 39.0 33.0 39.0 14 38.04668487391206 40.0 37.0 41.0 33.0 41.0 15 38.157396576510614 40.0 37.0 41.0 33.0 41.0 16 38.328516194541145 40.0 37.0 41.0 34.0 41.0 17 38.252777965576314 40.0 37.0 41.0 34.0 41.0 18 38.2384528348579 40.0 37.0 41.0 34.0 41.0 19 38.25048058708799 40.0 37.0 41.0 34.0 41.0 20 37.993212464777145 40.0 37.0 41.0 33.0 41.0 21 38.05319432556443 40.0 37.0 41.0 33.0 41.0 22 38.01230200896246 40.0 37.0 41.0 34.0 41.0 23 37.96412466818124 40.0 36.0 41.0 34.0 41.0 24 37.9206479602315 40.0 36.0 41.0 33.0 41.0 25 37.906606015534415 40.0 36.0 41.0 33.0 41.0 26 37.69884569805992 40.0 36.0 41.0 33.0 41.0 27 37.61994905202842 40.0 36.0 41.0 33.0 41.0 28 37.572970787193356 40.0 36.0 41.0 33.0 41.0 29 37.541620053652274 40.0 36.0 41.0 33.0 41.0 30 37.487549382156985 40.0 36.0 41.0 33.0 41.0 31 37.526972192918564 40.0 36.0 41.0 33.0 41.0 32 37.36649246049109 40.0 35.0 41.0 33.0 41.0 33 37.33488405318285 40.0 35.0 41.0 32.0 41.0 34 37.179159804933306 40.0 35.0 41.0 32.0 41.0 35 37.13306171286723 40.0 35.0 41.0 32.0 41.0 36 37.1002044807172 40.0 35.0 41.0 32.0 41.0 37 37.05077703948149 40.0 35.0 41.0 32.0 41.0 38 36.96221507595636 40.0 35.0 41.0 31.0 41.0 39 36.839555984728364 39.0 35.0 41.0 31.0 41.0 40 36.771797988869004 39.0 35.0 41.0 31.0 41.0 41 36.68575536044351 39.0 35.0 41.0 31.0 41.0 42 36.647257368895716 39.0 35.0 41.0 31.0 41.0 43 36.568313525193986 39.0 35.0 41.0 31.0 41.0 44 36.56175942709682 39.0 35.0 41.0 31.0 41.0 45 36.47513384366346 39.0 35.0 41.0 31.0 41.0 46 36.428110932383596 39.0 35.0 41.0 31.0 41.0 47 36.31513374161444 39.0 35.0 41.0 31.0 41.0 48 36.26725872104519 39.0 35.0 41.0 31.0 41.0 49 36.2565997012515 39.0 35.0 41.0 31.0 41.0 50 36.1148663731907 38.0 35.0 41.0 30.0 41.0 51 35.16581434477516 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 8.0 9 21.0 10 25.0 11 29.0 12 23.0 13 24.0 14 27.0 15 38.0 16 51.0 17 107.0 18 191.0 19 439.0 20 891.0 21 1648.0 22 2717.0 23 3687.0 24 4734.0 25 5327.0 26 6172.0 27 6928.0 28 7610.0 29 8820.0 30 10811.0 31 14060.0 32 18367.0 33 26511.0 34 50141.0 35 57925.0 36 55937.0 37 83634.0 38 145057.0 39 271695.0 40 276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54996128515429 20.370514467361538 22.921229639626652 11.158294607857519 2 30.738311879653597 29.081801216169158 22.540586807358245 17.639300096819007 3 31.337594730188776 28.751927769705983 23.654069140760036 16.256408359345205 4 28.131724870748542 23.86237669608655 28.789941028424476 19.215957404740433 5 29.327994468943295 27.633470546740362 22.96000826597035 20.07852671834599 6 25.521821268800938 36.53048650593096 23.374072151206025 14.573620074062074 7 76.15611968818922 6.207896808034318 12.659435643425429 4.976547860351023 8 75.8221642810583 9.590311466355077 9.093332755055572 5.494191497531052 9 69.87997760024084 7.754194533489299 10.426603158161944 11.939224708107922 10 43.76652715715676 23.70636926181568 17.319759113296094 15.207344467731462 11 33.10368052534834 24.093900402710933 23.385297543042363 19.417121528898367 12 27.87213768453332 21.215735448129124 27.91499827154478 22.997128595792773 13 27.33484961163971 21.923190256359888 30.705911316853268 20.036048815147137 14 21.31752934228133 28.268087869305823 28.56785685584428 21.84652593256856 15 19.575169943503113 24.556182448334496 33.66023035014293 22.20841725801946 16 23.43402594851372 25.26631604325348 27.81639340916426 23.483264599068548 17 24.089308196959706 26.26410030397851 27.12756254648014 22.51902895258165 18 24.045682242323043 24.730813828152005 27.900328725395024 23.323175204129925 19 24.288686463325497 26.033724648791928 26.03168366845805 23.64590521942452 20 26.425082627813207 26.767967323904855 27.618418316778005 19.188531731503936 21 24.82138233046788 28.31630602969371 27.437281312146254 19.42503032769215 22 23.37598557026904 22.863699506465444 29.82433537388846 23.935979549377056 23 24.137526357347593 25.85003641874283 28.251249781551323 21.761187442358253 24 23.94069931639915 25.500135852753473 27.12603181122973 23.433133019617646 25 22.649013887595558 29.029628656384375 25.43546228842369 22.885895167596377 26 21.605690253170852 25.92147073042859 27.729013938620067 24.743825077780485 27 24.120178024509624 26.114726055792747 26.523559928922857 23.24153599077477 28 19.35397869981899 27.969849618017772 28.876172447530863 23.799999234632374 29 22.043225412246137 26.5469036414916 28.25010173011352 23.159769216148746 30 22.7283569980751 26.964921926124163 27.490474362097977 22.81624671370276 31 24.191867458737118 24.674304185157737 26.67829175048505 24.455536605620097 32 25.148194306430238 26.444216818443316 27.118378134977682 21.28921074014876 33 24.736043840257572 24.032798553965435 27.24249525153169 23.988662354245303 34 23.658151101427794 25.225368875305033 27.9074721565636 23.209007866703573 35 22.84061091643844 25.41518004635577 26.523687490193726 25.22052154701207 36 23.702287301147923 28.119478988745268 26.48886326324692 21.689370446859886 37 22.839080181188027 25.192075383608632 28.487110571385198 23.48173386381814 38 22.14195783589753 24.877636850920418 27.798662392513684 25.18174292066837 39 24.64088313219047 24.08841526806363 28.456623427647887 22.81407817209801 40 23.357744308534997 22.99432224783369 29.312942238980938 24.334991204650375 41 22.990112725895067 24.648919492255118 26.32890142957916 26.032066352270654 42 22.203697490997364 23.6538140182183 28.679855651665886 25.46263283911845 43 24.065071555494892 23.530972514372966 28.08860916119535 24.31534676893679 44 22.80923084380505 23.197017107242036 28.55803463798749 25.43571741096542 45 21.726745899224046 22.819945990557915 28.004163599881117 27.449144510336925 46 24.335629011004713 24.942693099062808 27.04464772041631 23.677030169516172 47 21.219944970067747 24.459873688829585 31.213094929822166 23.1070864112805 48 21.905969484792784 24.430024351446608 28.467211013129884 25.196795150630724 49 22.49504743365857 22.565461255177393 31.24613329897683 23.693358012187204 50 21.89716775710293 22.90566716458083 29.86821645106686 25.328948627249382 51 21.571758955120117 22.31709946079851 27.325537638866386 28.785603945214987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 278.0 1 668.0 2 1058.0 3 2086.5 4 3115.0 5 2171.5 6 1228.0 7 1213.0 8 1198.0 9 1204.5 10 1211.0 11 1230.0 12 1249.0 13 1219.0 14 1189.0 15 1292.5 16 1396.0 17 1444.0 18 1492.0 19 1497.5 20 1503.0 21 1743.5 22 1984.0 23 2059.0 24 2134.0 25 3025.5 26 4644.0 27 5371.0 28 6586.0 29 7801.0 30 9756.0 31 11711.0 32 12888.0 33 14065.0 34 16104.5 35 18144.0 36 19872.0 37 21600.0 38 23481.0 39 25362.0 40 28410.0 41 31458.0 42 34907.0 43 38356.0 44 42277.0 45 46198.0 46 65786.5 47 85375.0 48 79208.0 49 73041.0 50 72119.5 51 71198.0 52 64115.5 53 57033.0 54 52144.5 55 47256.0 56 45202.5 57 43149.0 58 40899.5 59 38650.0 60 36277.0 61 33904.0 62 31138.0 63 28372.0 64 24432.0 65 20492.0 66 17654.0 67 14816.0 68 12570.0 69 10324.0 70 8528.5 71 6733.0 72 5647.0 73 4561.0 74 3720.0 75 2218.0 76 1557.0 77 1201.0 78 845.0 79 639.0 80 433.0 81 317.0 82 201.0 83 121.0 84 41.0 85 40.5 86 40.0 87 25.0 88 10.0 89 6.0 90 2.0 91 2.0 92 2.0 93 3.0 94 4.0 95 2.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 783937.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.44884585167059 #Duplication Level Percentage of deduplicated Percentage of total 1 71.40158528064602 21.026942784946563 2 10.353897036748586 6.09820635598556 3 3.788242337850122 3.346780939683615 4 1.9015714392356151 2.239963367599561 5 1.2166538475885051 1.7914525806237906 6 0.9228722346467495 1.6306513307339339 7 0.730397907284501 1.505656276740284 8 0.6232545926391063 1.4683302739979833 9 0.5826667343164291 1.5442976557602743 >10 8.341656165376815 50.695469094593214 >50 0.07934069870902996 1.5305958955406855 >100 0.05128652894052657 2.7593841471578497 >500 0.0017533856048043274 0.3471175136464941 >1k 0.0035067712096086548 1.3842101520384367 >5k 0.0013150392036032454 2.6309416309517277 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC 7406 0.9447187720441822 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC 6973 0.8894847417585852 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG 6002 0.7656227477463112 No Hit GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC 2134 0.2722157520310943 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1581 0.2016743692414059 No Hit GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT 1338 0.17067698042062054 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT 1292 0.1648091619607188 No Hit GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT 1263 0.16110988510556334 No Hit GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC 1064 0.13572519220294488 No Hit CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC 1044 0.1331739667855963 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA 1007 0.1284541997635014 No Hit CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT 816 0.1040899970278224 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.551225417348588E-4 0.0 0.0 0.10855464150818242 0.0 2 2.551225417348588E-4 0.0 0.0 0.6235194919999949 0.0 3 2.551225417348588E-4 0.0 0.0 0.8990518370736424 0.0 4 2.551225417348588E-4 0.0 0.0 1.4341713683625088 0.0 5 2.551225417348588E-4 0.0 0.0 3.3937676114279594 0.0 6 2.551225417348588E-4 0.0 0.0 4.1124478114950564 0.0 7 2.551225417348588E-4 0.0 0.0 4.87845324305397 0.0 8 2.551225417348588E-4 0.0 0.0 5.942824487171801 0.0 9 2.551225417348588E-4 0.0 0.0 6.307649721852649 0.0 10 2.551225417348588E-4 0.0 0.0 7.851651344432014 0.0 11 2.551225417348588E-4 0.0 0.0 8.972021986460646 0.0 12 2.551225417348588E-4 0.0 0.0 10.803674274846065 0.0 13 2.551225417348588E-4 0.0 0.0 11.225136713792052 0.0 14 3.826838126022882E-4 0.0 0.0 11.424387418886976 0.0 15 3.826838126022882E-4 0.0 0.0 11.863708435754404 0.0 16 3.826838126022882E-4 0.0 0.0 12.46924178856209 0.0 17 3.826838126022882E-4 0.0 0.0 13.137790409178288 0.0 18 3.826838126022882E-4 0.0 0.0 13.831085916342767 0.0 19 3.826838126022882E-4 0.0 0.0 14.496062821374677 0.0 20 3.826838126022882E-4 0.0 0.0 14.996868370800204 0.0 21 3.826838126022882E-4 0.0 0.0 15.572042141141443 0.0 22 3.826838126022882E-4 0.0 0.0 16.218012416814105 0.0 23 3.826838126022882E-4 0.0 0.0 16.755555612249452 0.0 24 3.826838126022882E-4 0.0 0.0 17.210439104162706 0.0 25 3.826838126022882E-4 0.0 0.0 17.64606084417498 0.0 26 3.826838126022882E-4 0.0 0.0 18.060507413223256 0.0 27 3.826838126022882E-4 0.0 0.0 18.52738166459805 0.0 28 3.826838126022882E-4 0.0 0.0 18.94259360127153 0.0 29 5.102450834697176E-4 0.0 0.0 19.361249692258433 0.0 30 6.37806354337147E-4 0.0 0.0 19.90134411311113 0.0 31 6.37806354337147E-4 0.0 0.0 20.35788590154566 0.0 32 6.37806354337147E-4 0.0 0.0 20.79554862189181 0.0 33 6.37806354337147E-4 0.0 0.0 21.232445974612755 0.0 34 6.37806354337147E-4 0.0 0.0 21.695876071674128 0.0 35 7.653676252045763E-4 0.0 0.0 22.15471396298427 0.0 36 8.929288960720057E-4 0.0 0.0 22.59645864399818 0.0 37 8.929288960720057E-4 0.0 0.0 23.061291915039092 0.0 38 8.929288960720057E-4 0.0 0.0 23.55992892285987 0.0 39 8.929288960720057E-4 0.0 0.0 24.204240902011257 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTGCG 20 7.031911E-4 45.000004 1 GCGATCG 30 2.1646138E-6 45.000004 33 CCGCGTA 20 7.031911E-4 45.000004 40 CGGTCGT 20 7.031911E-4 45.000004 29 CGATCGC 30 2.1646138E-6 45.000004 34 TTCGAAT 20 7.031911E-4 45.000004 16 CTACGCG 25 3.8896673E-5 45.0 1 ATAACGG 45 3.8380676E-10 45.0 2 GCGTAAC 25 3.8896673E-5 45.0 32 CCGTCGA 100 0.0 42.75 41 CCGATGA 950 0.0 42.39474 18 CGAATAT 60 3.6379788E-12 41.250004 14 TACGGGA 115 0.0 41.08696 4 CGATGAA 1030 0.0 40.631065 19 TACGGCT 805 0.0 40.52795 7 GCGATAT 50 1.0804797E-9 40.5 9 CGTAAGC 50 1.0804797E-9 40.5 43 CACCCGT 90 0.0 40.0 38 CGTTTTT 930 0.0 39.67742 1 GATGAAT 1095 0.0 39.65753 20 >>END_MODULE