##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935916.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368433
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04004527281758	33.0	31.0	34.0	30.0	34.0
2	32.15576780581544	33.0	31.0	34.0	30.0	34.0
3	32.21170199194969	34.0	31.0	34.0	30.0	34.0
4	35.79095792179311	37.0	35.0	37.0	35.0	37.0
5	35.81902544017501	37.0	35.0	37.0	35.0	37.0
6	35.853039765710456	37.0	35.0	37.0	35.0	37.0
7	36.02245455754506	37.0	35.0	37.0	35.0	37.0
8	35.693857499192525	37.0	35.0	37.0	35.0	37.0
9	37.44773405205289	39.0	37.0	39.0	34.0	39.0
10	37.21887561646215	39.0	37.0	39.0	34.0	39.0
11	37.15100710305537	39.0	37.0	39.0	34.0	39.0
12	37.03469558915732	39.0	37.0	39.0	34.0	39.0
13	37.00952411971783	39.0	37.0	39.0	33.0	39.0
14	38.16010780793251	40.0	37.0	41.0	33.0	41.0
15	38.28606286624705	40.0	37.0	41.0	34.0	41.0
16	38.389329946014605	40.0	37.0	41.0	34.0	41.0
17	38.34329986727573	40.0	37.0	41.0	34.0	41.0
18	38.35152660049453	40.0	37.0	41.0	34.0	41.0
19	38.35795110644269	40.0	37.0	41.0	34.0	41.0
20	38.20333683464836	40.0	37.0	41.0	34.0	41.0
21	38.20047335607831	40.0	37.0	41.0	34.0	41.0
22	38.1959189323432	40.0	37.0	41.0	34.0	41.0
23	38.225891817508206	40.0	37.0	41.0	34.0	41.0
24	38.13192357904965	40.0	37.0	41.0	34.0	41.0
25	38.062480830978764	40.0	36.0	41.0	34.0	41.0
26	37.89217306810193	40.0	36.0	41.0	34.0	41.0
27	37.81738877896388	40.0	36.0	41.0	33.0	41.0
28	37.81209066505986	40.0	36.0	41.0	33.0	41.0
29	37.79954292910787	40.0	36.0	41.0	33.0	41.0
30	37.63860186248246	40.0	36.0	41.0	33.0	41.0
31	37.65220270714078	40.0	36.0	41.0	33.0	41.0
32	37.57517920490292	40.0	35.0	41.0	33.0	41.0
33	37.5129969356708	40.0	35.0	41.0	33.0	41.0
34	37.47832306009505	40.0	35.0	41.0	33.0	41.0
35	37.456984037803345	40.0	35.0	41.0	33.0	41.0
36	37.38761728726797	40.0	35.0	41.0	33.0	41.0
37	37.29378747289195	39.0	35.0	41.0	33.0	41.0
38	37.24531190202832	39.0	35.0	41.0	33.0	41.0
39	37.22608723974237	39.0	35.0	41.0	33.0	41.0
40	37.17350508776358	39.0	35.0	41.0	33.0	41.0
41	37.07579668487893	39.0	35.0	41.0	32.0	41.0
42	37.06617485404402	39.0	35.0	41.0	32.0	41.0
43	37.00546096576583	39.0	35.0	41.0	32.0	41.0
44	36.93010398091376	39.0	35.0	41.0	32.0	41.0
45	36.830843056946584	39.0	35.0	41.0	32.0	41.0
46	36.781528798994664	39.0	35.0	41.0	32.0	41.0
47	36.722812560221264	39.0	35.0	41.0	31.0	41.0
48	36.68850781553227	39.0	35.0	41.0	31.0	41.0
49	36.662755507785675	39.0	35.0	41.0	31.0	41.0
50	36.54213113374752	39.0	35.0	41.0	31.0	41.0
51	35.506295581557566	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	9.0
11	10.0
12	8.0
13	5.0
14	11.0
15	12.0
16	20.0
17	32.0
18	58.0
19	124.0
20	242.0
21	425.0
22	596.0
23	884.0
24	1243.0
25	1729.0
26	2306.0
27	2746.0
28	3268.0
29	3961.0
30	4793.0
31	6351.0
32	8516.0
33	12201.0
34	23527.0
35	31203.0
36	25479.0
37	39131.0
38	71145.0
39	128274.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55997698360353	22.362274823373586	23.767414970971657	11.310333222051227
2	31.50260698688771	26.32391778152337	23.86838312529008	18.30509210629884
3	32.77719422527298	25.07131554448163	25.791120773654914	16.36036945659048
4	29.748421015489928	24.609901936037218	26.652607122597594	18.98906992587526
5	26.392315563481013	29.864317257140378	23.941395043332168	19.801972136046444
6	25.92845917710954	34.42389796788019	24.504862485173696	15.142780369836577
7	77.34784886261545	6.234512109393024	10.702352937983296	5.715286090008224
8	76.39217985359618	6.909804496339904	9.875879739328452	6.822135910735466
9	69.77985142481809	8.747044917257684	12.370227422625009	9.102876235299227
10	40.79574848072784	25.077015359644765	19.405427852553924	14.72180830707347
11	31.4257951920702	24.876707569625957	24.637586752543882	19.05991048575996
12	29.324463335260415	20.54403378633293	29.41783173602799	20.71367114237867
13	24.783610588628054	24.4258793321988	29.98129917786952	20.80921090130363
14	19.039011163495125	29.570641066353993	28.717840150040846	22.672507620110032
15	18.682637005914238	25.88611769304053	35.82116694215773	19.610078358887503
16	20.526662921073846	25.724894349854655	30.783887436793123	22.96455529227838
17	19.544666194396267	26.735390152347914	28.194814253880622	25.52512939937519
18	21.02906091473891	26.59153767442113	30.07792461587317	22.301476794966792
19	24.11483227615333	26.71964780570687	26.651792863288577	22.51372705485122
20	24.975775785556667	27.090407211080443	28.285468456951467	19.648348546411423
21	23.34861426636593	27.675316814726152	28.435835009350413	20.540233909557504
22	20.88520843681212	25.632068788626427	27.411496798603817	26.071225975957635
23	21.98907264007296	26.809487749468698	27.93017997844927	23.271259632009077
24	23.33287191972489	24.22720006079803	28.303653581519573	24.13627443795751
25	21.928274611666165	27.014681095341626	26.266919629891998	24.79012466310021
26	20.705528549288474	27.347984572500287	28.286825555799833	23.659661322411402
27	23.539693784215853	26.39882963795317	28.52594637288191	21.53553020494907
28	20.852366644681666	25.885574853501183	30.034768872495132	23.22728962932202
29	24.35965290839854	24.199786664061037	28.68472693814072	22.755833489399702
30	24.62971557922336	25.54277168440395	28.127230731232	21.70028200514069
31	25.995228440449146	26.97152535196359	24.90249244774491	22.13075375984236
32	27.1940895630956	26.275876482291217	26.329074757147158	20.200959197466027
33	24.993418070585424	27.155547955802007	25.76045033968184	22.090583633930727
34	24.497534151392518	26.497897853883885	26.906656027011692	22.0979119677119
35	25.43963217192814	23.86322614966629	27.662017246012166	23.035124432393406
36	26.47754137115839	27.985278191692924	25.6212119978395	19.915968439309182
37	24.330882412813185	27.017395293038355	30.061368009923108	18.590354284225356
38	24.659843173657084	26.381187352924414	26.463970382674734	22.49499909074377
39	25.71105194160132	25.17445505695744	27.833554540445615	21.28093846099562
40	25.80876305868367	24.001650232199612	28.46894822125054	21.72063848786618
41	21.99015831915165	27.728243669812418	26.173551229124424	24.1080467819115
42	23.74922984640355	25.949358499374377	28.19318573526258	22.108225918959484
43	25.47627384083402	24.146316969435418	28.407878773074074	21.96953041665649
44	23.276416607632868	24.11564653546235	28.049333257335796	24.558603599568983
45	23.424883221644098	23.134735487863463	27.055936900331943	26.38444439016049
46	25.809577317992687	25.131299313579404	27.2193316016752	21.839791766752708
47	22.620395024332783	23.612434282488266	30.99640911644722	22.770761576731726
48	22.801432010704794	22.966998070205438	29.271807899943813	24.959762019145952
49	23.508209090933764	22.77781849074323	31.297956480554127	22.416015937768876
50	22.571268046021935	21.762165712626178	30.172107275949767	25.494458965402124
51	21.3216514264466	22.48821359650194	27.208746230657948	28.98138874639351
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	175.0
1	471.5
2	768.0
3	1009.0
4	1250.0
5	911.5
6	573.0
7	568.0
8	563.0
9	567.0
10	571.0
11	573.5
12	576.0
13	584.0
14	592.0
15	634.5
16	677.0
17	689.0
18	701.0
19	680.5
20	660.0
21	799.0
22	938.0
23	1035.5
24	1133.0
25	1574.5
26	2375.0
27	2734.0
28	3298.0
29	3862.0
30	4669.0
31	5476.0
32	6431.5
33	7387.0
34	7434.5
35	7482.0
36	7995.0
37	8508.0
38	9505.0
39	10502.0
40	13060.5
41	15619.0
42	17706.0
43	19793.0
44	22576.0
45	25359.0
46	31287.5
47	37216.0
48	40056.5
49	42897.0
50	42326.5
51	41756.0
52	35114.5
53	28473.0
54	24718.0
55	20963.0
56	18997.0
57	17031.0
58	15858.0
59	14685.0
60	14210.5
61	13736.0
62	12377.0
63	11018.0
64	8943.5
65	6869.0
66	6198.5
67	5528.0
68	4431.5
69	3335.0
70	2847.5
71	2360.0
72	2161.5
73	1963.0
74	1555.0
75	971.0
76	795.0
77	602.0
78	409.0
79	321.0
80	233.0
81	163.5
82	94.0
83	48.5
84	3.0
85	4.0
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.165993159000834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79957941596038	22.451727939949166
2	12.550201405875697	8.073793851309606
3	4.78331093307806	4.615798402522883
4	2.344997866615992	3.017167413417661
5	1.3938389479074456	2.2417107031569907
6	0.9023584271188656	1.7415152996203307
7	0.6733318843503846	1.5160872150026128
8	0.5461205599757964	1.4053208156936938
9	0.44859773113766815	1.2986632395825777
>10	6.34820431257983	43.86292445849709
>50	0.13822565996023906	2.8924625993210196
>100	0.06360076378538607	3.8725284347944657
>500	0.002544030551415443	0.44898089763130883
>1k	0.005088061102830886	2.5613187295006012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2127	0.5773098500948612	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1936	0.5254686740872831	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	1472	0.39952990095892604	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	1301	0.3531171203448117	Illumina Single End Adapter 1 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	1301	0.3531171203448117	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	1253	0.3400889714004989	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	587	0.15932340479815868	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.14738093493253862	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	516	0.14005260115136267	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	470	0.12756729174639622	Illumina Single End Adapter 1 (95% over 22bp)
TGCGAAGGACATAGGCTTCATTAAGTTGGACTAAGCGACCTTGAATGGATT	432	0.11725334049881525	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	393	0.1066679694815611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.428395393463669E-4	0.0	0.0	0.36858804721618316	0.0
2	5.428395393463669E-4	0.0	0.0	1.8214980742767342	0.0
3	5.428395393463669E-4	0.0	0.0	2.4821337936612626	0.0
4	5.428395393463669E-4	0.0	0.0	3.332220512277673	0.0
5	5.428395393463669E-4	0.0	0.0	5.844753320142333	0.0
6	5.428395393463669E-4	0.0	0.0	6.99638740286565	0.0
7	5.428395393463669E-4	0.0	0.0	8.21479074892857	0.0
8	5.428395393463669E-4	0.0	0.0	10.145670990383598	0.0
9	5.428395393463669E-4	0.0	0.0	10.804678191150087	0.0
10	8.142593090195504E-4	0.0	0.0	12.587906077902902	0.0
11	8.142593090195504E-4	0.0	0.0	14.684352378858572	0.0
12	8.142593090195504E-4	0.0	0.0	16.725157627031237	0.0
13	8.142593090195504E-4	0.0	0.0	17.419177978085568	0.0
14	8.142593090195504E-4	0.0	0.0	17.686797870983327	0.0
15	8.142593090195504E-4	0.0	0.0	18.224480434705903	0.0
16	8.142593090195504E-4	0.0	0.0	19.248547225682824	0.0
17	8.142593090195504E-4	0.0	0.0	20.465322053127707	0.0
18	8.142593090195504E-4	0.0	0.0	21.779265158115585	0.0
19	8.142593090195504E-4	0.0	0.0	22.640208667518923	0.0
20	8.142593090195504E-4	0.0	0.0	23.42596890072279	0.0
21	8.142593090195504E-4	0.0	0.0	24.416922479799585	0.0
22	8.142593090195504E-4	0.0	0.0	25.414390133348533	0.0
23	8.142593090195504E-4	0.0	0.0	26.30654691626429	0.0
24	8.142593090195504E-4	0.0	0.0	27.05865109802868	0.0
25	8.142593090195504E-4	0.0	0.0	27.6940447788336	0.0
26	8.142593090195504E-4	0.0	0.0	28.28655413603016	0.0
27	8.142593090195504E-4	0.0	0.0	28.880420592075087	0.0
28	8.142593090195504E-4	0.0	0.0	29.507943099559487	0.0
29	8.142593090195504E-4	0.0	0.0	30.122437458099572	0.0
30	8.142593090195504E-4	0.0	0.0	30.79230144965299	0.0
31	8.142593090195504E-4	0.0	0.0	31.47519359015072	0.0
32	8.142593090195504E-4	0.0	0.0	32.1019018383261	0.0
33	8.142593090195504E-4	0.0	0.0	32.69766823275874	0.0
34	8.142593090195504E-4	0.0	0.0	33.306191356366014	0.0
35	8.142593090195504E-4	0.0	0.0	33.9206857149061	0.0
36	8.142593090195504E-4	0.0	0.0	34.52948025828305	0.0
37	8.142593090195504E-4	0.0	0.0	35.134203505114904	0.0
38	8.142593090195504E-4	0.0	0.0	35.75032638227303	0.0
39	8.142593090195504E-4	0.0	0.0	36.370520555976256	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	35	1.2089185E-7	45.000004	1
GTCGAGG	20	7.02704E-4	45.0	2
CGAAAGG	25	3.8856288E-5	45.0	2
CGACGGT	25	3.8856288E-5	45.0	28
ACGTTAG	20	7.02704E-4	45.0	1
GCGGTAG	20	7.02704E-4	45.0	1
CCGATAC	25	3.8856288E-5	45.0	11
CCCTACA	25	3.8856288E-5	45.0	27
TTGTACC	50	2.1827873E-11	45.0	20
GCCCTCG	50	2.1827873E-11	45.0	13
ACGTGCG	25	3.8856288E-5	45.0	1
ACGTGAG	25	3.8856288E-5	45.0	44
TCCACCG	20	7.02704E-4	45.0	18
ATGACGG	20	7.02704E-4	45.0	2
CTACCGG	25	3.8856288E-5	45.0	2
ACGTCCG	20	7.02704E-4	45.0	15
TATAGCG	25	3.8856288E-5	45.0	1
TGTAGCG	25	3.8856288E-5	45.0	1
CGATGGA	20	7.02704E-4	45.0	10
ACGTAAG	20	7.02704E-4	45.0	1
>>END_MODULE