FastQCFastQC Report
Sat 14 Jan 2017
SRR2935915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174437
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC116700.9936676041371313No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC105640.8994948217741777No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG92430.7870153954618255No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83610.7119155816787107No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC32310.27511054232794097No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT25410.21635898732754502No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG23750.20222455525498603No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC21880.1863020323780671No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT21560.18357732258094728No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT20220.1721676003055081No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT16700.14219579253719017No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC16160.13759784475455047No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC16020.13640578421831057No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC15760.13419195750815072No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA15420.13129695334871092No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT15220.12959400972551105No Hit
CGTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT12880.10966956933407242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG351.2119563E-745.01
CCATACG3700.043.7837832
CGTTTTT42650.042.4677621
TACGGCT13300.042.1240587
AACCCGC3700.041.95945721
CGATGAA16450.040.89665619
GAACCCG3800.040.85526320
CTAAACG501.0822987E-940.51
CCGATGA16050.040.37383318
CTATGCG451.9283107E-840.01
CCCGCAT3900.039.80769323
TGCGACG403.458299E-739.3751
GATGAAT17950.039.35933320
TGAATGA17900.039.34357522
ACGGCTG14450.039.0830428
TAGCCGT1100.038.86363244
CCGAACC4000.038.812518
GTTTACG356.248405E-638.5714261
ACCATAC4150.038.4939771
CGTATGG1350.038.3333322
Produced by FastQC (version 0.11.5)