##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935915.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174437
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09661906087768	33.0	31.0	34.0	30.0	34.0
2	32.22658005495399	33.0	31.0	34.0	30.0	34.0
3	32.26995743492414	34.0	31.0	34.0	30.0	34.0
4	35.84530971009939	37.0	35.0	37.0	35.0	37.0
5	35.84985231221428	37.0	35.0	37.0	35.0	37.0
6	35.86905300156586	37.0	35.0	37.0	35.0	37.0
7	36.02302209484204	37.0	35.0	37.0	35.0	37.0
8	35.658959995299874	37.0	35.0	37.0	34.0	37.0
9	37.45507677295589	39.0	37.0	39.0	34.0	39.0
10	37.13387520999424	39.0	37.0	39.0	33.0	39.0
11	37.11765978081413	39.0	37.0	39.0	34.0	39.0
12	36.98350017923482	39.0	37.0	39.0	33.0	39.0
13	36.92912263493061	39.0	37.0	39.0	33.0	39.0
14	38.05137355175288	40.0	37.0	41.0	33.0	41.0
15	38.17776517599496	40.0	37.0	41.0	33.0	41.0
16	38.32534397332509	40.0	37.0	41.0	34.0	41.0
17	38.238209456956824	40.0	37.0	41.0	33.0	41.0
18	38.21622615772493	40.0	37.0	41.0	34.0	41.0
19	38.174057016255446	40.0	37.0	41.0	34.0	41.0
20	37.88379112715284	40.0	36.0	41.0	33.0	41.0
21	37.957399162322034	40.0	36.0	41.0	33.0	41.0
22	37.94368280290897	40.0	36.0	41.0	34.0	41.0
23	37.876118514658515	40.0	36.0	41.0	34.0	41.0
24	37.83849197530391	40.0	36.0	41.0	33.0	41.0
25	37.83507587039577	40.0	36.0	41.0	33.0	41.0
26	37.61798972614112	40.0	35.0	41.0	33.0	41.0
27	37.55621544620954	40.0	35.0	41.0	33.0	41.0
28	37.53709224079282	40.0	35.0	41.0	33.0	41.0
29	37.46074502080571	40.0	35.0	41.0	33.0	41.0
30	37.382153321123226	40.0	35.0	41.0	33.0	41.0
31	37.360152140983296	40.0	35.0	41.0	32.0	41.0
32	37.14738040439802	40.0	35.0	41.0	32.0	41.0
33	37.055722869766534	40.0	35.0	41.0	32.0	41.0
34	36.822778914492645	40.0	35.0	41.0	31.0	41.0
35	36.738587084705266	40.0	35.0	41.0	31.0	41.0
36	36.71619678194744	40.0	35.0	41.0	31.0	41.0
37	36.67149451183844	40.0	35.0	41.0	31.0	41.0
38	36.57366295510104	39.0	35.0	41.0	31.0	41.0
39	36.467457173096555	39.0	35.0	41.0	30.0	41.0
40	36.42955135098775	39.0	35.0	41.0	30.0	41.0
41	36.32695751240807	39.0	35.0	41.0	30.0	41.0
42	36.32451123389335	39.0	35.0	41.0	30.0	41.0
43	36.252934810466634	39.0	35.0	41.0	30.0	41.0
44	36.2572483666642	39.0	35.0	41.0	30.0	41.0
45	36.145280674910616	39.0	35.0	41.0	30.0	41.0
46	36.08796044402552	39.0	35.0	41.0	30.0	41.0
47	35.9643480237765	39.0	35.0	41.0	29.0	41.0
48	35.93670669435653	39.0	35.0	41.0	29.0	41.0
49	35.91411374130754	39.0	35.0	41.0	29.0	41.0
50	35.761800760704915	38.0	35.0	41.0	29.0	41.0
51	34.8468593887965	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	11.0
9	25.0
10	24.0
11	31.0
12	39.0
13	30.0
14	37.0
15	52.0
16	80.0
17	148.0
18	352.0
19	651.0
20	1220.0
21	2376.0
22	4065.0
23	6065.0
24	7977.0
25	9800.0
26	12248.0
27	13568.0
28	14344.0
29	14990.0
30	17371.0
31	21578.0
32	27980.0
33	40275.0
34	75839.0
35	84947.0
36	82042.0
37	120765.0
38	218948.0
39	396236.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45568387235757	19.770323993539034	22.595848053152277	13.17814408095113
2	32.71763406636541	27.88706418479663	22.99144185682161	16.403859892016346
3	30.231080934950107	27.7560226729914	26.04533065630596	15.967565735752535
4	26.551786089845603	23.948070437154144	31.80357907661288	17.696564396387377
5	28.144719554986775	27.351318120937947	25.513756804324117	18.990205519751164
6	24.333276284721954	35.69761511260289	25.549944356317113	14.419164246358044
7	74.99653025236773	5.34639150503603	14.430573968633482	5.2265042739627585
8	75.94319661250455	8.285927640222507	10.70061655073878	5.0702591965341695
9	70.61766616685271	6.160824292831374	12.045345982798567	11.176163557517347
10	42.04729585324713	21.451555085543113	20.239910697636397	16.261238363573355
11	32.47794475140003	23.487679628622054	24.746836143616047	19.287539476361864
12	26.97862890900065	21.221572549229972	28.530947168728503	23.26885137304087
13	26.384386731685055	21.04923465456214	32.30117920331189	20.265199410440918
14	21.051107892547662	26.325890618228136	30.45842390864729	22.164577580576903
15	20.22552082402036	23.184215074967835	34.55766465123289	22.032599449778914
16	23.318321885294825	24.626438029455816	28.689065484142617	23.366174601106742
17	23.469117543129176	25.84199918769589	29.386676339386447	21.302206929788486
18	24.00741802242266	24.55534013318722	29.100752105051185	22.336489739338937
19	22.97807374937949	26.69576997318715	27.26548976232867	23.060666515104682
20	25.246735244206377	26.999404821203694	28.780428409527286	18.973431525062647
21	23.23870927091023	28.930117154006556	29.06005175245671	18.7711218226265
22	22.141076958576747	22.782235232711503	31.968934902425588	23.107752906286162
23	23.159181803706797	25.41132474538864	30.131458732992915	21.298034717911644
24	23.42194600476654	25.112713581060543	28.651856165975698	22.81348424819722
25	22.2224776637657	29.073590154261147	26.448587706279692	22.25534447569346
26	20.910615043633673	25.05489864505291	29.729989773823544	24.30449653748988
27	23.27617403062063	24.8418603977906	28.19103962153781	23.69092595005096
28	19.79961462385807	27.18749494438612	29.05502806876827	23.95786236298754
29	21.79103689682801	26.41546545280845	28.6309951065915	23.16250254377204
30	23.179106244098236	26.188803656560548	28.108021119906816	22.5240689794344
31	24.959533802153715	25.028332724530987	25.90041015397165	24.11172331934365
32	25.886871752167206	27.41722203915578	25.09304458221258	21.602861626464424
33	25.965292306015563	23.81524083454455	25.889596461964327	24.329870397475556
34	22.783682734791224	25.48727603098336	29.03127200522463	22.697769229000787
35	21.886827475633005	25.722367398166103	27.75202075547688	24.638784370724014
36	23.441955592339138	27.499644510518657	27.061051380363526	21.99734851677868
37	21.884443354560528	26.379192753634296	28.17716063100873	23.559203260796448
38	24.43153613178059	25.48480676272972	25.93140372791389	24.152253377575807
39	22.746473416624305	25.78214071934042	28.47338767426435	22.99799818977093
40	23.26859593149739	23.356467822454505	29.298804448429333	24.076131797618775
41	20.425701846927506	24.13590511879309	29.830463447592336	25.607929586687067
42	21.25758980686065	23.619828053782367	30.24155403823279	24.881028101124198
43	22.598487615768235	23.36302415540382	29.34044142001657	24.698046808811373
44	22.959171075161972	23.196305974692557	28.613369640091378	25.231153310054093
45	22.354966677650655	22.770995804798382	27.86833180494143	27.00570571260953
46	24.131477465372768	24.953403205110195	27.217126163429796	23.69799316608724
47	20.416846540086866	24.379170615367194	31.590540829350573	23.613442015195364
48	21.41119532167328	24.474280016722908	29.18828340728366	24.926241254320157
49	22.643275033058394	22.473661848187685	31.114823528209683	23.768239590544233
50	21.95060271432184	22.69334157558047	30.279699975392464	25.076355734705224
51	20.742279066480364	22.52304721326048	28.828366272520366	27.906307447738786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	539.0
1	934.0
2	1329.0
3	7512.0
4	13695.0
5	8893.5
6	4092.0
7	3965.5
8	3839.0
9	3866.5
10	3894.0
11	3802.0
12	3710.0
13	3742.5
14	3775.0
15	3593.5
16	3412.0
17	3253.5
18	3095.0
19	2907.5
20	2720.0
21	3054.0
22	3388.0
23	3672.5
24	3957.0
25	4754.5
26	6676.5
27	7801.0
28	8641.5
29	9482.0
30	11094.5
31	12707.0
32	15002.0
33	17297.0
34	20534.0
35	23771.0
36	25783.5
37	27796.0
38	31336.0
39	34876.0
40	39317.0
41	43758.0
42	48748.5
43	53739.0
44	61361.0
45	68983.0
46	100107.5
47	131232.0
48	123559.5
49	115887.0
50	115667.0
51	115447.0
52	103427.5
53	91408.0
54	83859.5
55	76311.0
56	70660.5
57	65010.0
58	58657.0
59	52304.0
60	49012.5
61	45721.0
62	41423.0
63	37125.0
64	33595.5
65	30066.0
66	24611.5
67	19157.0
68	16634.0
69	14111.0
70	11849.5
71	9588.0
72	7867.5
73	6147.0
74	4925.0
75	2887.0
76	2071.0
77	1524.0
78	977.0
79	795.0
80	613.0
81	436.0
82	259.0
83	161.5
84	64.0
85	36.5
86	9.0
87	12.0
88	15.0
89	8.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1174437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.241599783851697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06707096085057	21.794235175924833
2	10.31462696246293	6.238616410370596
3	3.8655388143662943	3.507002333190302
4	2.045126840436796	2.4739162966281083
5	1.2680922989399788	1.9174569896763631
6	0.8904489376242899	1.615716023975382
7	0.7351652125451141	1.5562800492959725
8	0.617247745010821	1.4933247417681752
9	0.547822360509635	1.4910322121259572
>10	7.5042211010923925	47.73852800593523
>50	0.10011334970379777	1.9846497940635186
>100	0.03743538199368724	2.099198676656646
>500	0.001985209649841772	0.40258369768314894
>1k	0.003970419299683544	2.270729863625703
>5k	5.67202757097649E-4	1.5098175147026318
>10k+	5.67202757097649E-4	1.9069122143773185
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	11670	0.9936676041371313	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	10564	0.8994948217741777	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	9243	0.7870153954618255	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8361	0.7119155816787107	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	3231	0.27511054232794097	No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	2541	0.21635898732754502	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	2375	0.20222455525498603	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	2188	0.1863020323780671	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT	2156	0.18357732258094728	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	2022	0.1721676003055081	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	1670	0.14219579253719017	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1616	0.13759784475455047	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	1602	0.13640578421831057	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC	1576	0.13419195750815072	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA	1542	0.13129695334871092	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	1522	0.12959400972551105	No Hit
CGTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	1288	0.10966956933407242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5544154347998234E-4	0.0	0.0	0.13495828213859065	0.0
2	2.5544154347998234E-4	0.0	0.0	0.6418394515840355	0.0
3	2.5544154347998234E-4	0.0	0.0	1.0596566695361267	0.0
4	2.5544154347998234E-4	0.0	0.0	1.7176740855405612	0.0
5	2.5544154347998234E-4	0.0	0.0	3.8220866679098155	0.0
6	2.5544154347998234E-4	0.0	0.0	4.949265052105818	0.0
7	2.5544154347998234E-4	0.0	0.0	5.990870519236026	0.0
8	2.5544154347998234E-4	0.0	0.0	7.337643483643652	0.0
9	2.5544154347998234E-4	0.0	0.0	7.954619958328969	0.0
10	2.5544154347998234E-4	0.0	0.0	9.784773470181882	0.0
11	2.5544154347998234E-4	0.0	0.0	11.009956259893038	0.0
12	2.5544154347998234E-4	0.0	0.0	12.904140451978266	0.0
13	2.5544154347998234E-4	0.0	0.0	13.406764262365712	0.0
14	2.5544154347998234E-4	0.0	0.0	13.671486848592133	0.0
15	2.5544154347998234E-4	0.0	0.0	14.109058212573343	0.0
16	3.4058872463997645E-4	0.0	0.0	14.707642896128101	0.0
17	3.4058872463997645E-4	0.0	0.0	15.350248672342579	0.0
18	3.4058872463997645E-4	0.0	0.0	16.000858283586094	0.0
19	3.4058872463997645E-4	0.0	0.0	16.655129223619486	0.0
20	4.257359057999705E-4	0.0	0.0	17.149664051796734	0.0
21	4.257359057999705E-4	0.0	0.0	17.712231477720813	0.0
22	4.257359057999705E-4	0.0	0.0	18.35602931447153	0.0
23	4.257359057999705E-4	0.0	0.0	18.94243795112041	0.0
24	4.257359057999705E-4	0.0	0.0	19.431608506884576	0.0
25	4.257359057999705E-4	0.0	0.0	19.861857213286026	0.0
26	4.257359057999705E-4	0.0	0.0	20.277034868622156	0.0
27	4.257359057999705E-4	0.0	0.0	20.744322598828205	0.0
28	6.811774492799529E-4	0.0	0.0	21.173549539055735	0.0
29	6.811774492799529E-4	0.0	0.0	21.632322551145784	0.0
30	7.66324630439947E-4	0.0	0.0	22.166110229837788	0.0
31	8.51471811599941E-4	0.0	0.0	22.631354427695992	0.0
32	9.366189927599352E-4	0.0	0.0	23.101452014880323	0.0
33	9.366189927599352E-4	0.0	0.0	23.56439723884721	0.0
34	9.366189927599352E-4	0.0	0.0	24.020700982683618	0.0
35	0.0010217661739199294	0.0	0.0	24.490713422686785	0.0
36	0.0010217661739199294	0.0	0.0	24.921643306537515	0.0
37	0.0010217661739199294	0.0	0.0	25.39046368600444	0.0
38	0.0010217661739199294	0.0	0.0	25.923570187247165	0.0
39	0.0010217661739199294	0.0	0.0	26.637614448454876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	35	1.2119563E-7	45.0	1
CCATACG	370	0.0	43.783783	2
CGTTTTT	4265	0.0	42.467762	1
TACGGCT	1330	0.0	42.124058	7
AACCCGC	370	0.0	41.959457	21
CGATGAA	1645	0.0	40.896656	19
GAACCCG	380	0.0	40.855263	20
CTAAACG	50	1.0822987E-9	40.5	1
CCGATGA	1605	0.0	40.373833	18
CTATGCG	45	1.9283107E-8	40.0	1
CCCGCAT	390	0.0	39.807693	23
TGCGACG	40	3.458299E-7	39.375	1
GATGAAT	1795	0.0	39.359333	20
TGAATGA	1790	0.0	39.343575	22
ACGGCTG	1445	0.0	39.083042	8
TAGCCGT	110	0.0	38.863632	44
CCGAACC	400	0.0	38.8125	18
GTTTACG	35	6.248405E-6	38.571426	1
ACCATAC	415	0.0	38.493977	1
CGTATGG	135	0.0	38.333332	2
>>END_MODULE