SRR2935914.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935914.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515887
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1468	0.2845584401235154	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1274	0.24695330566577564	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	876	0.16980462775762908	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.1482882879390254	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	606	0.11746758495562012	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	527	0.10215415391355083	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	20	7.0296664E-4	45.000004	27
CGTTAGG	40	6.8030204E-9	45.000004	2
TATTGCG	20	7.0296664E-4	45.000004	1
TCATGCG	20	7.0296664E-4	45.000004	23
TCGTCTG	20	7.0296664E-4	45.000004	18
CAGTACG	20	7.0296664E-4	45.000004	1
CGATATA	20	7.0296664E-4	45.000004	10
ATACCGG	30	2.1631658E-6	45.000004	2
GACGTAA	25	3.887806E-5	45.0	21
ATAGCCG	25	3.887806E-5	45.0	1
CTAACGG	25	3.887806E-5	45.0	2
GCCGAAC	25	3.887806E-5	45.0	11
AATAGCG	25	3.887806E-5	45.0	1
CGTATGG	50	1.0786607E-9	40.5	2
ATATGCG	40	3.4532786E-7	39.375004	1
AATACGG	40	3.4532786E-7	39.375004	2
CAGTTGG	75	0.0	39.0	2
CGAGGGA	225	0.0	39.0	4
ACATACG	75	0.0	39.0	17
GGGTACG	35	6.2415947E-6	38.571426	7