##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935914.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515887
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15169794935713	33.0	31.0	34.0	30.0	34.0
2	32.272063455756786	34.0	31.0	34.0	30.0	34.0
3	32.31400674954011	34.0	31.0	34.0	30.0	34.0
4	35.85899819146441	37.0	35.0	37.0	35.0	37.0
5	35.88628905167217	37.0	35.0	37.0	35.0	37.0
6	35.90800117079903	37.0	35.0	37.0	35.0	37.0
7	36.056985347566425	37.0	35.0	37.0	35.0	37.0
8	35.76642365479262	37.0	35.0	37.0	35.0	37.0
9	37.505064093493345	39.0	37.0	39.0	35.0	39.0
10	37.2615398333356	39.0	37.0	39.0	34.0	39.0
11	37.20062339233204	39.0	37.0	39.0	34.0	39.0
12	37.08552260475647	39.0	37.0	39.0	34.0	39.0
13	37.043172245084676	39.0	37.0	39.0	33.0	39.0
14	38.18039609449356	40.0	37.0	41.0	33.0	41.0
15	38.32295250704127	40.0	37.0	41.0	34.0	41.0
16	38.42943706664444	40.0	38.0	41.0	34.0	41.0
17	38.39963596679118	40.0	37.0	41.0	34.0	41.0
18	38.38180648087663	40.0	37.0	41.0	34.0	41.0
19	38.38263805833448	40.0	37.0	41.0	34.0	41.0
20	38.27038285516014	40.0	37.0	41.0	34.0	41.0
21	38.24214605136396	40.0	37.0	41.0	34.0	41.0
22	38.232081831874034	40.0	37.0	41.0	34.0	41.0
23	38.25697100333988	40.0	37.0	41.0	34.0	41.0
24	38.195982841203595	40.0	37.0	41.0	34.0	41.0
25	38.110768443476964	40.0	37.0	41.0	34.0	41.0
26	37.95082837908301	40.0	36.0	41.0	34.0	41.0
27	37.91264172192748	40.0	36.0	41.0	34.0	41.0
28	37.8772405584944	40.0	36.0	41.0	34.0	41.0
29	37.85545090300783	40.0	36.0	41.0	33.0	41.0
30	37.74374233116942	40.0	36.0	41.0	33.0	41.0
31	37.758947211307905	40.0	36.0	41.0	33.0	41.0
32	37.67778214996695	40.0	36.0	41.0	33.0	41.0
33	37.62089953807714	40.0	36.0	41.0	33.0	41.0
34	37.5412910191573	40.0	36.0	41.0	33.0	41.0
35	37.47522034864224	40.0	35.0	41.0	33.0	41.0
36	37.44126911513568	40.0	35.0	41.0	33.0	41.0
37	37.40153560760399	40.0	35.0	41.0	33.0	41.0
38	37.31239980848519	40.0	35.0	41.0	32.0	41.0
39	37.27984229104435	40.0	35.0	41.0	32.0	41.0
40	37.147908359776466	39.0	35.0	41.0	32.0	41.0
41	37.01115360534381	39.0	35.0	41.0	32.0	41.0
42	36.997123401054886	39.0	35.0	41.0	32.0	41.0
43	36.93727696181528	39.0	35.0	41.0	32.0	41.0
44	36.839484228135234	39.0	35.0	41.0	32.0	41.0
45	36.68581491683256	39.0	35.0	41.0	31.0	41.0
46	36.580417804674276	39.0	35.0	41.0	31.0	41.0
47	36.47410964028944	38.0	35.0	41.0	31.0	41.0
48	36.33884939919013	38.0	35.0	40.0	31.0	41.0
49	36.23589468236261	37.0	35.0	40.0	31.0	41.0
50	36.04804540529224	37.0	35.0	40.0	31.0	41.0
51	35.04915417523605	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	13.0
10	21.0
11	23.0
12	15.0
13	11.0
14	21.0
15	28.0
16	42.0
17	57.0
18	110.0
19	198.0
20	324.0
21	551.0
22	813.0
23	1267.0
24	1823.0
25	2399.0
26	3201.0
27	3877.0
28	4596.0
29	5515.0
30	6910.0
31	8979.0
32	11477.0
33	17079.0
34	33348.0
35	40366.0
36	36851.0
37	56076.0
38	105475.0
39	174254.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8211992161074	21.372509871347795	24.40844603566285	11.397844876881953
2	32.5075064888241	25.137093976006376	24.15916663920587	18.19623289596365
3	32.8405251537643	24.329358948762035	25.072351115651298	17.757764781822377
4	29.46090907504938	25.250490902077395	25.13089106722984	20.157708955643386
5	26.087108223312473	29.690610540680417	23.746285523767803	20.47599571223931
6	27.161762168847055	33.83744889869293	23.53131596648103	15.469472965978984
7	79.38211274949747	6.265131705199006	8.967080000077535	5.38567554522599
8	78.93996165827014	6.042214671042302	8.952735773531801	6.065087897155772
9	72.82544433180135	7.842415102532144	11.421299625693223	7.910840939973289
10	41.616284186265595	25.992513864470318	17.404198981559915	14.98700296770417
11	32.323163793621475	25.472051049939232	23.059701058565153	19.145084097874147
12	30.931579977785834	21.580113474462433	27.634540122158533	19.853766425593204
13	26.917522635771014	22.842017728688646	28.754746679020794	21.48571295651955
14	20.58687270661986	26.83068191289953	29.474090256199514	23.108355124281093
15	19.895829900734075	25.442974915049227	34.128985611190046	20.532209573026652
16	23.64955891503372	25.02098327734562	30.628994334030512	20.700463473590148
17	22.895711657785522	25.080686274319763	28.21024759298063	23.81335447491408
18	24.645901137264556	25.39005634954942	28.132711233273955	21.831331279912074
19	25.66065824492573	27.38293463491036	26.00433815932559	20.952068960838325
20	27.869862973868308	26.065010360796066	26.566864448997553	19.498262216338073
21	26.09524954108167	26.573455039572618	26.90007695483701	20.4312184645087
22	23.263234002795187	25.429406052100557	27.399217270448762	23.908142674655497
23	23.301032978152193	26.62307830978489	27.901265199549513	22.1746235125134
24	24.701533475354097	23.558647533277636	27.69637536902461	24.043443622343652
25	23.203337164921773	26.152626447264616	25.56238090899751	25.081655478816096
26	22.869155454585986	27.06968774169537	26.643819286006433	23.417337517712213
27	23.727482956538932	27.237553960460332	26.824866685921528	22.210096397079205
28	21.877077732139014	27.660320961761002	27.042937697596564	23.419663608503413
29	24.80174922027498	26.596715947484622	26.319911143331776	22.281623688908617
30	25.284994582146865	25.61898245158339	26.249159215099432	22.846863751170314
31	26.591869925002953	26.30207778059924	24.41891344422325	22.687138850174556
32	27.97705699116279	27.10380373996631	24.162655775392672	20.75648349347822
33	26.4495907049412	26.86363486577487	24.22565406765435	22.461120361629582
34	26.127233289460676	25.426886120410092	26.729884645280844	21.715995944848387
35	24.582515163204345	26.26195271445103	26.4321450240072	22.723387098337426
36	24.792251016210916	29.640211906871077	24.105472710108998	21.46206436680901
37	26.096800268275803	28.397110219873735	25.072932638349094	20.433156873501368
38	24.640279751185822	27.137919738237247	24.692422953088563	23.529377557488367
39	26.091954245794135	24.69087222589443	25.233626743841192	23.983546784470242
40	26.0869143824132	23.166507394061103	28.362412698904993	22.384165524620702
41	22.061226586442377	26.92217481735341	27.009403222023426	24.007195374180778
42	24.052166462810654	26.459670431703064	27.24433839193467	22.24382471355161
43	24.530178120402336	25.723656537187406	25.979914206017984	23.76625113639227
44	24.23088777193455	24.918829123432072	26.062684270004866	24.787598834628515
45	25.364275509946943	22.674733032621486	25.98262797860772	25.97836347882385
46	24.353007538472575	25.02156480004342	26.025660658244927	24.59976700323908
47	23.039735445940682	25.59029399849192	28.488409283428346	22.881561272139052
48	22.962586768032534	24.276052701463694	28.016212852814665	24.745147677689104
49	23.85522410915569	23.9168655151225	28.52523905428902	23.702671321432796
50	21.398290710950267	24.20859606851888	28.571954711012292	25.821158509518554
51	21.489008251807082	23.354145384551266	27.189287576542924	27.967558787098724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	558.0
2	871.0
3	1294.5
4	1718.0
5	1240.0
6	762.0
7	744.5
8	727.0
9	743.0
10	759.0
11	779.5
12	800.0
13	842.0
14	884.0
15	841.0
16	798.0
17	873.0
18	948.0
19	1007.5
20	1067.0
21	1211.5
22	1356.0
23	1537.0
24	1718.0
25	2222.0
26	3287.0
27	3848.0
28	4421.5
29	4995.0
30	6040.0
31	7085.0
32	7561.5
33	8038.0
34	9886.0
35	11734.0
36	12619.0
37	13504.0
38	15064.5
39	16625.0
40	18240.0
41	19855.0
42	21451.5
43	23048.0
44	25889.0
45	28730.0
46	30749.5
47	32769.0
48	36830.5
49	40892.0
50	46665.5
51	52439.0
52	51912.0
53	51385.0
54	45569.5
55	39754.0
56	36334.5
57	32915.0
58	30841.0
59	28767.0
60	27257.5
61	25748.0
62	23343.0
63	20938.0
64	17598.5
65	14259.0
66	11506.5
67	8754.0
68	7332.5
69	5911.0
70	4672.5
71	3434.0
72	2937.5
73	2441.0
74	1874.0
75	985.5
76	664.0
77	539.5
78	415.0
79	273.5
80	132.0
81	103.5
82	75.0
83	48.0
84	21.0
85	16.5
86	12.0
87	10.0
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	515887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.44368100798724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.02371263647291	23.04270676780679
2	10.83101413280889	7.027959350357063
3	3.9374760450515334	3.8323865034672937
4	2.056822005506539	2.669235081474509
5	1.2714560339624175	2.062535699077863
6	0.9292371499676381	1.808872420459275
7	0.8007072528761624	1.8184523485167192
8	0.6911359593641593	1.7938395679008012
9	0.6406148069900809	1.8705512202281547
>10	7.690475165940932	48.92787469381253
>50	0.08268619485809611	1.8208694512449244
>100	0.04104132299525938	2.244609798862305
>500	0.0024141954703093753	0.5431865639050888
>1k	0.0012070977351546876	0.5369205328867172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1468	0.2845584401235154	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1274	0.24695330566577564	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	876	0.16980462775762908	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.1482882879390254	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	606	0.11746758495562012	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	527	0.10215415391355083	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9384089926669987E-4	0.0	0.0	0.1763952183326969	0.0
2	1.9384089926669987E-4	0.0	0.0	0.8949634319143533	0.0
3	1.9384089926669987E-4	0.0	0.0	1.2752792762756184	0.0
4	1.9384089926669987E-4	0.0	0.0	1.7798471370668383	0.0
5	1.9384089926669987E-4	0.0	0.0	3.236367654156821	0.0
6	1.9384089926669987E-4	0.0	0.0	4.030727659351757	0.0
7	1.9384089926669987E-4	0.0	0.0	4.828382959834228	0.0
8	1.9384089926669987E-4	0.0	0.0	6.066832465249173	0.0
9	1.9384089926669987E-4	0.0	0.0	6.574307939529393	0.0
10	1.9384089926669987E-4	0.0	0.0	7.673967361069382	0.0
11	1.9384089926669987E-4	0.0	0.0	9.129906355461564	0.0
12	1.9384089926669987E-4	0.0	0.0	10.373201883358178	0.0
13	1.9384089926669987E-4	0.0	0.0	10.875055971559663	0.0
14	1.9384089926669987E-4	0.0	0.0	11.106695846183369	0.0
15	1.9384089926669987E-4	0.0	0.0	11.53489039266351	0.0
16	1.9384089926669987E-4	0.0	0.0	12.275168786963036	0.0
17	3.8768179853339974E-4	0.0	0.0	13.106552403917913	0.0
18	3.8768179853339974E-4	0.0	0.0	13.986202404790196	0.0
19	3.8768179853339974E-4	0.0	0.0	14.548922535361426	0.0
20	3.8768179853339974E-4	0.0	0.0	15.126568415176191	0.0
21	3.8768179853339974E-4	0.0	0.0	15.828466311420913	0.0
22	5.815226978000996E-4	0.0	0.0	16.599565408703842	0.0
23	5.815226978000996E-4	0.0	0.0	17.299524895955898	0.0
24	7.753635970667995E-4	0.0	0.0	17.852746822463057	0.0
25	9.692044963334994E-4	0.0	0.0	18.354213228866012	0.0
26	9.692044963334994E-4	0.0	0.0	18.834938659047427	0.0
27	9.692044963334994E-4	0.0	0.0	19.315664089228843	0.0
28	9.692044963334994E-4	0.0	0.0	19.81034606415746	0.0
29	9.692044963334994E-4	0.0	0.0	20.301151221100746	0.0
30	9.692044963334994E-4	0.0	0.0	20.87356339663531	0.0
31	9.692044963334994E-4	0.0	0.0	21.379294302822128	0.0
32	9.692044963334994E-4	0.0	0.0	21.904796980734154	0.0
33	9.692044963334994E-4	0.0	0.0	22.387654660807502	0.0
34	9.692044963334994E-4	0.0	0.0	22.86876777278745	0.0
35	9.692044963334994E-4	0.0	0.0	23.403574813864278	0.0
36	9.692044963334994E-4	0.0	0.0	23.931015900768966	0.0
37	9.692044963334994E-4	0.0	0.0	24.464078373752393	0.0
38	9.692044963334994E-4	0.0	0.0	25.008383618893284	0.0
39	9.692044963334994E-4	0.0	0.0	25.672094857982465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	20	7.0296664E-4	45.000004	27
CGTTAGG	40	6.8030204E-9	45.000004	2
TATTGCG	20	7.0296664E-4	45.000004	1
TCATGCG	20	7.0296664E-4	45.000004	23
TCGTCTG	20	7.0296664E-4	45.000004	18
CAGTACG	20	7.0296664E-4	45.000004	1
CGATATA	20	7.0296664E-4	45.000004	10
ATACCGG	30	2.1631658E-6	45.000004	2
GACGTAA	25	3.887806E-5	45.0	21
ATAGCCG	25	3.887806E-5	45.0	1
CTAACGG	25	3.887806E-5	45.0	2
GCCGAAC	25	3.887806E-5	45.0	11
AATAGCG	25	3.887806E-5	45.0	1
CGTATGG	50	1.0786607E-9	40.5	2
ATATGCG	40	3.4532786E-7	39.375004	1
AATACGG	40	3.4532786E-7	39.375004	2
CAGTTGG	75	0.0	39.0	2
CGAGGGA	225	0.0	39.0	4
ACATACG	75	0.0	39.0	17
GGGTACG	35	6.2415947E-6	38.571426	7
>>END_MODULE