##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935912.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306583
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00364338531491	33.0	31.0	34.0	30.0	34.0
2	32.12258996748026	33.0	31.0	34.0	30.0	34.0
3	32.195085833200146	34.0	31.0	34.0	30.0	34.0
4	35.752709706669975	37.0	35.0	37.0	33.0	37.0
5	35.79350453221477	37.0	35.0	37.0	35.0	37.0
6	35.82517947831419	37.0	35.0	37.0	35.0	37.0
7	35.98856427133924	37.0	35.0	37.0	35.0	37.0
8	35.67467537338992	37.0	35.0	37.0	35.0	37.0
9	37.41760306344448	39.0	37.0	39.0	34.0	39.0
10	37.215563159079274	39.0	37.0	39.0	34.0	39.0
11	37.18470039108496	39.0	37.0	39.0	34.0	39.0
12	37.07902264639592	39.0	37.0	39.0	34.0	39.0
13	37.01828868528261	39.0	37.0	39.0	33.0	39.0
14	38.180580788889145	40.0	37.0	41.0	33.0	41.0
15	38.34490496863818	40.0	37.0	41.0	34.0	41.0
16	38.44191948020601	40.0	38.0	41.0	34.0	41.0
17	38.42096593744598	40.0	37.0	41.0	34.0	41.0
18	38.41856202072522	40.0	37.0	41.0	34.0	41.0
19	38.41633423901521	40.0	37.0	41.0	34.0	41.0
20	38.27310711944237	40.0	37.0	41.0	34.0	41.0
21	38.23478796932641	40.0	37.0	41.0	34.0	41.0
22	38.22801003317209	40.0	37.0	41.0	34.0	41.0
23	38.25566322985945	40.0	37.0	41.0	34.0	41.0
24	38.16707710473183	40.0	37.0	41.0	34.0	41.0
25	38.08050348518998	40.0	36.0	41.0	34.0	41.0
26	37.92936986069025	40.0	36.0	41.0	34.0	41.0
27	37.84999494427284	40.0	36.0	41.0	33.0	41.0
28	37.86366497816252	40.0	36.0	41.0	33.0	41.0
29	37.86741600153955	40.0	36.0	41.0	33.0	41.0
30	37.73932670761262	40.0	36.0	41.0	33.0	41.0
31	37.75515276450423	40.0	36.0	41.0	33.0	41.0
32	37.70191106486661	40.0	36.0	41.0	33.0	41.0
33	37.62693952371789	40.0	36.0	41.0	33.0	41.0
34	37.553093289582264	40.0	36.0	41.0	33.0	41.0
35	37.56579131915338	40.0	36.0	41.0	33.0	41.0
36	37.46749493611844	40.0	35.0	41.0	33.0	41.0
37	37.384199384832165	40.0	35.0	41.0	33.0	41.0
38	37.32868423885212	40.0	35.0	41.0	33.0	41.0
39	37.261932331538276	39.0	35.0	41.0	33.0	41.0
40	37.13825293639895	39.0	35.0	41.0	32.0	41.0
41	37.0876728324793	39.0	35.0	41.0	32.0	41.0
42	37.03095083549969	39.0	35.0	41.0	32.0	41.0
43	36.919711790934265	39.0	35.0	41.0	32.0	41.0
44	36.83869947126879	39.0	35.0	41.0	31.0	41.0
45	36.74718754790709	39.0	35.0	41.0	31.0	41.0
46	36.68241552858443	39.0	35.0	41.0	31.0	41.0
47	36.645466317440956	39.0	35.0	41.0	31.0	41.0
48	36.571437424775674	39.0	35.0	41.0	31.0	41.0
49	36.515508035344425	39.0	35.0	41.0	31.0	41.0
50	36.33707022241938	38.0	35.0	40.0	31.0	41.0
51	35.256475407964565	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	8.0
11	5.0
12	9.0
13	5.0
14	5.0
15	12.0
16	26.0
17	42.0
18	61.0
19	93.0
20	169.0
21	294.0
22	459.0
23	649.0
24	989.0
25	1467.0
26	1918.0
27	2376.0
28	2747.0
29	3431.0
30	4208.0
31	5235.0
32	7033.0
33	9779.0
34	18372.0
35	26009.0
36	21931.0
37	33699.0
38	60536.0
39	104912.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.230325882387476	21.962078784537955	24.73653137975687	11.071063953317699
2	31.74148599237401	24.62824096574174	25.107719606109928	18.522553435774324
3	32.75100054471383	24.293258269375666	26.363496997550417	16.592244188360088
4	29.68462047797823	25.43552643166777	26.470156531836402	18.409696558517595
5	26.142349706278562	30.40188138285554	23.764853237133174	19.690915673732725
6	25.651781083752194	34.69403065401539	23.61807406151026	16.036114200722153
7	77.80992422932779	6.862415724289997	9.479325337673648	5.848334708708572
8	76.87901807993268	6.593972920872977	9.526620849818809	7.000388149375536
9	70.1509868453241	9.041923394317362	12.481448743080993	8.325641017277539
10	39.649621798990815	26.996278332458097	18.44296650499212	14.911133363558971
11	29.982092940574006	25.15305806258012	24.427642759057093	20.43720623778879
12	27.352788641248864	22.671837642661206	29.097177599540746	20.878196116549187
13	23.727669179308702	25.461946683279894	30.8764673840363	19.933916753375104
14	19.284500445230165	30.0877739470225	28.61737278322673	22.010352824520606
15	18.855579076465425	25.226447650391577	34.828088967750986	21.089884305392015
16	19.956749069583115	26.067655414683788	31.443687353832406	22.531908161900695
17	19.54152709054318	26.055260728742297	28.4386283649126	25.964583815801916
18	21.04356732108434	26.95028752409625	29.207751245176674	22.79839390964274
19	23.095866372238515	27.322128102340965	27.045857076224056	22.536148449196467
20	24.12234207376143	27.25297880182528	27.783993241634402	20.84068588277889
21	22.61051656484541	27.99046261534397	28.661406535913603	20.737614283897017
22	21.222637915344293	25.39769002195164	26.65999093230871	26.719681130395358
23	20.059494492519157	26.485160625344523	28.229875759582235	25.225469122554088
24	21.70276890760414	25.327236017652645	28.817644813965547	24.15235026077767
25	20.32989435161115	27.649934927898805	27.527945124158876	24.492225596331174
26	20.558543689637062	27.91218038834508	27.873365450791464	23.655910471226388
27	22.59486011944563	28.215850193911603	26.838082998731178	22.351206687911592
28	18.45144707958367	28.854828871790023	28.961488406076008	23.732235642550304
29	21.559903843331167	26.073200405762876	28.287608901993917	24.07928684891204
30	21.806819034323492	28.60171633782695	25.893151283665432	23.69831334418412
31	22.12418822961482	30.589106375761215	23.87575305871493	23.410952335909034
32	22.533539041629837	28.776546644791136	25.573498856753307	23.11641545682572
33	22.040034835591015	28.993779824713044	23.828783722515603	25.137401617180338
34	20.292710293786676	29.137623416823505	26.162898790865768	24.406767498524054
35	21.62481285655108	25.271786106861764	26.023295486051083	27.08010555053607
36	23.744956504437624	29.12131461953207	24.165071122664987	22.96865775336532
37	20.62965004582772	30.6298783689898	26.685758832029173	22.054712753153304
38	20.389910725643627	28.210305202832515	25.870645143403255	25.52913892812061
39	23.257323465423717	24.609322760883675	26.86939588953073	25.263957884161876
40	22.63497976078256	25.330823953056758	27.90533069348268	24.128865592678004
41	21.246448759389793	26.645639190692243	25.068904668556314	27.039007381361653
42	23.86107514115264	25.414977347080566	26.336750569992468	24.38719694177433
43	24.21954250561838	25.169366859871552	27.553060671987684	23.058029962522383
44	21.44248050283284	26.34588349647567	26.276081844068326	25.935554156623162
45	21.540333286581447	25.063359677477226	26.02166460632194	27.374642429619385
46	23.158165977891795	26.591820159630508	25.813564352883233	24.436449509594464
47	21.19621766373217	25.900653330419495	28.543983195415272	24.359145810433063
48	20.982572419214375	24.793285994331065	27.740285664893356	26.48385592156121
49	22.934735454999135	22.64998385429068	30.08842629891416	24.326854391796022
50	21.029867931359533	22.70086730183996	29.517618393713935	26.75164637308657
51	20.264659162445405	23.23188174164908	25.63286287889413	30.870596217011382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	247.0
1	391.0
2	535.0
3	876.0
4	1217.0
5	883.0
6	549.0
7	533.5
8	518.0
9	509.0
10	500.0
11	531.0
12	562.0
13	558.5
14	555.0
15	559.0
16	563.0
17	548.0
18	533.0
19	515.0
20	497.0
21	683.5
22	870.0
23	945.5
24	1021.0
25	1337.0
26	2029.5
27	2406.0
28	3009.5
29	3613.0
30	4080.5
31	4548.0
32	5030.0
33	5512.0
34	5776.0
35	6040.0
36	6380.5
37	6721.0
38	7767.0
39	8813.0
40	10386.5
41	11960.0
42	14322.5
43	16685.0
44	19945.0
45	23205.0
46	26962.0
47	30719.0
48	34635.0
49	38551.0
50	37250.5
51	35950.0
52	29784.5
53	23619.0
54	20582.5
55	17546.0
56	15429.5
57	13313.0
58	12537.5
59	11762.0
60	10836.0
61	9910.0
62	8694.0
63	7478.0
64	6620.5
65	5763.0
66	4888.5
67	4014.0
68	3521.0
69	3028.0
70	2527.5
71	2027.0
72	1828.5
73	1630.0
74	1302.0
75	711.0
76	448.0
77	370.5
78	293.0
79	207.0
80	121.0
81	93.5
82	66.0
83	41.0
84	16.0
85	17.5
86	19.0
87	13.0
88	7.0
89	6.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	306583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.336435375648904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.68457027428613	22.896987382529552
2	12.924238013532523	8.616960506352639
3	4.974309697978778	4.974772613553994
4	2.436058816936071	3.2483806928827614
5	1.4168197492362016	2.3615860004677858
6	1.020147267910213	2.040484408220016
7	0.782345866735247	1.8256435699477915
8	0.6374669911038925	1.7000701722435414
9	0.5065764326511567	1.519870725891184
>10	6.446609749643968	43.268184777560805
>50	0.10691060806128155	2.444850126382225
>100	0.059949873679223296	3.6243071151450943
>500	9.991645613203885E-4	0.19219053445811224
>1k	0.002997493683961165	1.2857113743644943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1560	0.5088344754927703	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1190	0.3881493755361517	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1110	0.3620552998698558	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	577	0.18820352074315927	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	484	0.15786915778109029	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	474	0.1546073983228033	No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	440	0.14351741616462751	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	381	0.1242730353607343	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	353	0.11514010887753072	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	324	0.10568100644849844	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	322	0.10502865455684104	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	315	0.10274542293604016	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.37673321743214727	0.0
2	0.0	0.0	0.0	1.6220729786061197	0.0
3	0.0	0.0	0.0	2.256811369188768	0.0
4	0.0	0.0	0.0	3.090843262672751	0.0
5	0.0	0.0	0.0	5.353199622940607	0.0
6	0.0	0.0	0.0	6.601474967627038	0.0
7	0.0	0.0	0.0	7.86181882230913	0.0
8	0.0	0.0	0.0	9.98783363722059	0.0
9	0.0	0.0	0.0	10.775548546396898	0.0
10	0.0	0.0	0.0	12.516675745230492	0.0
11	0.0	0.0	0.0	14.824370561968538	0.0
12	0.0	0.0	0.0	16.734783076687226	0.0
13	0.0	0.0	0.0	17.48074746479746	0.0
14	0.0	0.0	0.0	17.785069622255637	0.0
15	0.0	0.0	0.0	18.384907186634614	0.0
16	0.0	0.0	0.0	19.503690680827052	0.0
17	0.0	0.0	0.0	20.882436403844963	0.0
18	0.0	0.0	0.0	22.401111607623385	0.0
19	0.0	0.0	0.0	23.215899120303472	0.0
20	0.0	0.0	0.0	24.06721833891638	0.0
21	0.0	0.0	0.0	25.05748851045231	0.0
22	0.0	0.0	0.0	26.065698359008817	0.0
23	0.0	0.0	0.0	27.076517615131955	0.0
24	0.0	0.0	0.0	27.891305127812043	0.0
25	0.0	0.0	0.0	28.586712244318832	0.0
26	0.0	0.0	0.0	29.244935303001146	0.0
27	0.0	0.0	0.0	29.845099043325952	0.0
28	0.0	0.0	0.0	30.472661563100367	0.0
29	0.0	0.0	0.0	31.1038120182789	0.0
30	0.0	0.0	0.0	31.881741649080347	0.0
31	0.0	0.0	0.0	32.63390338016133	0.0
32	0.0	0.0	0.0	33.31528493099748	0.0
33	0.0	0.0	0.0	33.930778940776236	0.0
34	0.0	0.0	0.0	34.52083122678035	0.0
35	0.0	0.0	0.0	35.149046098446426	0.0
36	0.0	0.0	0.0	35.785741544704045	0.0
37	0.0	0.0	0.0	36.39308115583708	0.0
38	3.261759458286989E-4	0.0	0.0	36.999768415078464	0.0
39	3.261759458286989E-4	0.0	0.0	37.57938307081606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	45	3.8198777E-10	45.000004	4
ACAACGG	20	7.025186E-4	45.0	2
TTAGGCG	20	7.025186E-4	45.0	1
TTGGACG	35	1.2080272E-7	45.0	1
GGTCGAT	20	7.025186E-4	45.0	8
GCGCGCC	25	3.8840924E-5	45.0	16
TTTCGTG	20	7.025186E-4	45.0	15
CGTGACG	20	7.025186E-4	45.0	18
AAGACGC	20	7.025186E-4	45.0	38
GCAAACG	20	7.025186E-4	45.0	10
TTCGTGA	20	7.025186E-4	45.0	16
TTCGTAC	20	7.025186E-4	45.0	25
GTCTAGT	20	7.025186E-4	45.0	44
TTCAAGT	20	7.025186E-4	45.0	39
TGTTGCG	20	7.025186E-4	45.0	1
TCGTGAC	20	7.025186E-4	45.0	17
CGGGCAT	20	7.025186E-4	45.0	6
GGACGTA	20	7.025186E-4	45.0	8
TCGTACA	20	7.025186E-4	45.0	26
CTCGAAT	25	3.8840924E-5	45.0	39
>>END_MODULE