##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935910.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	604174
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.105568925508216	33.0	31.0	34.0	30.0	34.0
2	32.21952947329743	33.0	31.0	34.0	30.0	34.0
3	32.27281544720561	34.0	31.0	34.0	30.0	34.0
4	35.82429896023331	37.0	35.0	37.0	35.0	37.0
5	35.866376242605604	37.0	35.0	37.0	35.0	37.0
6	35.90192063875639	37.0	35.0	37.0	35.0	37.0
7	36.08379043123339	37.0	35.0	37.0	35.0	37.0
8	35.8478368814282	37.0	35.0	37.0	35.0	37.0
9	37.59313873155747	39.0	38.0	39.0	35.0	39.0
10	37.183447152641456	39.0	37.0	39.0	34.0	39.0
11	37.13087289423245	39.0	37.0	39.0	34.0	39.0
12	37.10265420226624	39.0	37.0	39.0	34.0	39.0
13	37.137606053885136	39.0	37.0	39.0	34.0	39.0
14	38.3398507714665	40.0	38.0	41.0	34.0	41.0
15	38.462156597271644	40.0	38.0	41.0	34.0	41.0
16	38.538937458414296	40.0	38.0	41.0	35.0	41.0
17	38.490486184443554	40.0	38.0	41.0	34.0	41.0
18	38.46723460460066	40.0	38.0	41.0	34.0	41.0
19	38.475957257346394	40.0	37.0	41.0	34.0	41.0
20	38.33694763429078	40.0	37.0	41.0	34.0	41.0
21	38.33050081599009	40.0	37.0	41.0	34.0	41.0
22	38.33188121302803	40.0	37.0	41.0	34.0	41.0
23	38.34303031908026	40.0	37.0	41.0	34.0	41.0
24	38.265559259418644	40.0	37.0	41.0	34.0	41.0
25	38.17452588161689	40.0	37.0	41.0	34.0	41.0
26	38.05732454557793	40.0	37.0	41.0	34.0	41.0
27	38.033056702208306	40.0	37.0	41.0	34.0	41.0
28	38.035880392072485	40.0	37.0	41.0	34.0	41.0
29	38.01940169553804	40.0	37.0	41.0	34.0	41.0
30	37.867139267826815	40.0	36.0	41.0	33.0	41.0
31	37.875489511299726	40.0	36.0	41.0	34.0	41.0
32	37.85245641156356	40.0	36.0	41.0	34.0	41.0
33	37.800532628017756	40.0	36.0	41.0	33.0	41.0
34	37.658196148791575	40.0	36.0	41.0	33.0	41.0
35	37.65543369956337	40.0	36.0	41.0	33.0	41.0
36	37.59118234151089	40.0	36.0	41.0	33.0	41.0
37	37.54601820005495	40.0	36.0	41.0	33.0	41.0
38	37.54355864370198	40.0	36.0	41.0	33.0	41.0
39	37.44193560133339	40.0	35.0	41.0	33.0	41.0
40	37.34215805380569	40.0	35.0	41.0	33.0	41.0
41	37.250796955843846	39.0	35.0	41.0	32.0	41.0
42	37.27405019083906	39.0	35.0	41.0	33.0	41.0
43	37.272060697746014	39.0	35.0	41.0	33.0	41.0
44	37.21155660455432	39.0	35.0	41.0	33.0	41.0
45	37.0961974530516	39.0	35.0	41.0	32.0	41.0
46	37.024456530734525	39.0	35.0	41.0	32.0	41.0
47	36.9953076431624	39.0	35.0	41.0	32.0	41.0
48	36.962245975497126	39.0	35.0	41.0	32.0	41.0
49	36.93626835977715	39.0	35.0	41.0	32.0	41.0
50	36.818858474545415	39.0	35.0	41.0	32.0	41.0
51	35.87143273295441	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	5.0
9	15.0
10	12.0
11	9.0
12	10.0
13	16.0
14	20.0
15	13.0
16	49.0
17	55.0
18	90.0
19	178.0
20	283.0
21	458.0
22	779.0
23	1171.0
24	1642.0
25	2536.0
26	3404.0
27	4111.0
28	4991.0
29	6129.0
30	7556.0
31	9624.0
32	12833.0
33	18420.0
34	35808.0
35	45943.0
36	41095.0
37	62692.0
38	122565.0
39	221428.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.893143365983974	22.949183513358733	25.58335843647691	11.574314684180385
2	30.602607858001175	25.518641980621474	26.67294521114778	17.20580495022957
3	32.080327852572275	24.499895725403608	27.080774743699664	16.339001678324454
4	28.52307447854426	26.11681403039522	26.695786313214402	18.664325177846116
5	25.409732957724103	29.744742408643866	25.218728379572774	19.62679625405926
6	26.228205781778097	33.95925677040058	24.293994776339268	15.518542671482056
7	80.90599727892958	5.686772353659707	8.252755001042745	5.154475366367967
8	82.28473916454531	4.598344185615402	7.960620615915283	5.1562960339240025
9	76.04829072419534	7.575135639732925	10.093449900194315	6.283123735877413
10	39.16206258461966	28.161092665357994	18.031229414042972	14.64561533597937
11	29.18993534975024	24.74452723884179	26.121613972133854	19.943923439274116
12	27.236028031659753	22.129717597910535	30.105896645668302	20.52835772476141
13	24.44229642453995	23.084409458202437	31.537603405641423	20.93569071161619
14	20.111259339196984	27.00712046529642	30.656069278055657	22.225550917450935
15	19.96875072412914	26.342245776878848	33.73663878286719	19.952364716124826
16	22.75950305706634	25.30363769377696	31.345440220863523	20.591419028293174
17	22.200227086898806	25.948319523845782	28.68991383277003	23.161539556485383
18	22.774895973676458	26.821412374580834	29.604716522061526	20.798975129681185
19	23.334999520005827	28.445944380261317	27.664712483489858	20.554343616243003
20	24.430544843041904	28.13196198446143	28.08909353927842	19.348399633218243
21	23.813338541545978	28.398110478107302	28.370138403837302	19.418412576509418
22	22.0280912452373	25.43737400152936	28.622218102731996	23.912316650501346
23	21.614137649087844	27.00579634343749	29.15782539467107	22.222240612803596
24	23.43463968989066	25.184135696008102	28.88174598708319	22.499478627018043
25	21.4376984113848	27.00083088646648	27.30587545971856	24.25559524243016
26	20.187065315621	27.274924111265957	28.468288936630838	24.069721636482207
27	21.05502719415268	27.583278989165372	28.41284133378795	22.948852482894
28	18.985424728637774	27.90090272007733	28.969800090702346	24.143872460582546
29	22.013029359091917	26.135351736420304	27.223283358767507	24.628335545720272
30	22.805019745967222	26.52182980399686	27.866972097442126	22.80617835259379
31	22.452968846722964	26.80171606192918	26.112676149586044	24.63263894176181
32	24.82447109607497	26.904335505996617	25.172053084045327	23.099140313883087
33	24.50866803271905	26.53722272060698	26.204206073084908	22.749903173589065
34	21.682164409590616	25.031034106068784	29.27600327058099	24.010798213759614
35	22.605077345268086	25.20002515831532	27.87839264847544	24.316504847941157
36	22.03785664394694	28.031659753647126	27.141187803513557	22.789295798892372
37	22.24607480626442	27.392274411014046	28.684120799637192	21.67752998308434
38	23.644016458834706	26.658048840234766	26.362438635227598	23.335496065702927
39	24.50966112411325	24.061776905328596	28.57951517278135	22.849046797776797
40	24.21123716015585	23.144822518016333	30.0380354003979	22.60590492142992
41	20.902918695607557	25.358092205225645	28.941496985967618	24.797492113199176
42	21.62075825838252	25.711136195864103	28.694713774508667	23.973391771244707
43	22.383783479593628	24.768361432302616	28.727320275284935	24.12053481281882
44	21.934078593252938	25.05768205847984	27.585761717650875	25.422477630616346
45	23.395412579819723	23.59303776726572	27.079781652305464	25.931768000609097
46	23.314641146424705	25.26176233998815	27.35387487710494	24.069721636482207
47	20.936352772545657	24.364338750095172	30.129234293432024	24.570074183927147
48	21.385726628421615	23.401371128184927	30.396872424169196	24.816029819224266
49	22.797902590975447	22.3899075431912	30.364596953857664	24.447592911975686
50	21.28492785191021	22.030739488955167	30.606083677880875	26.078248981253743
51	20.575198535521224	22.437410414880482	28.992972223233703	27.99441882636459
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	228.0
1	474.5
2	721.0
3	1465.5
4	2210.0
5	1560.0
6	910.0
7	898.0
8	886.0
9	907.5
10	929.0
11	936.5
12	944.0
13	977.5
14	1011.0
15	1024.0
16	1037.0
17	1071.0
18	1105.0
19	1128.5
20	1152.0
21	1533.0
22	1914.0
23	2174.5
24	2435.0
25	2964.5
26	4241.0
27	4988.0
28	6290.5
29	7593.0
30	9208.5
31	10824.0
32	11705.0
33	12586.0
34	14910.0
35	17234.0
36	18548.5
37	19863.0
38	20958.0
39	22053.0
40	24564.5
41	27076.0
42	29313.5
43	31551.0
44	33758.5
45	35966.0
46	39423.5
47	42881.0
48	49260.0
49	55639.0
50	59920.0
51	64201.0
52	62526.5
53	60852.0
54	52677.5
55	44503.0
56	38500.5
57	32498.0
58	29308.5
59	26119.0
60	24365.0
61	22611.0
62	20243.5
63	17876.0
64	14488.0
65	11100.0
66	8761.0
67	6422.0
68	5345.0
69	4268.0
70	3507.5
71	2747.0
72	2277.0
73	1807.0
74	1349.5
75	697.0
76	502.0
77	419.5
78	337.0
79	230.0
80	123.0
81	87.5
82	52.0
83	32.5
84	13.0
85	14.5
86	16.0
87	9.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	604174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.504616387054426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39752844987026	25.349418585964482
2	11.131763577961348	7.904579910938042
3	4.0525647504300135	4.316542705431498
4	2.174367358292432	3.0880031576282287
5	1.325985379461581	2.3539301116313114
6	1.0493984487538985	2.23550936161063
7	0.8485663154104409	2.1089615055336757
8	0.696949657469951	1.9795944183647673
9	0.6577770464019836	2.1018709530640916
>10	6.559702340439699	43.56509251602404
>50	0.07010761004913733	1.6982977098456344
>100	0.03152489847696899	1.9453744668924107
>500	0.00235260436395291	0.5786841694560163
>1k	0.0014115626183717458	0.7741404276152366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1692	0.2800517731646844	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1600	0.2648243717869355	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	1342	0.2221214418362922	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	998	0.16518420190210104	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	687	0.11370896463601546	No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	609	0.10079877651140234	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.310304647336694E-4	0.0	0.0	0.23155581008120177	0.0
2	3.310304647336694E-4	0.0	0.0	0.9947465465246766	0.0
3	3.310304647336694E-4	0.0	0.0	1.4093622036035975	0.0
4	3.310304647336694E-4	0.0	0.0	1.929742094164926	0.0
5	3.310304647336694E-4	0.0	0.0	3.4384134371886246	0.0
6	3.310304647336694E-4	0.0	0.0	4.399725906775201	0.0
7	3.310304647336694E-4	0.0	0.0	5.335549030577284	0.0
8	3.310304647336694E-4	0.0	0.0	6.877157904841982	0.0
9	3.310304647336694E-4	0.0	0.0	7.464736979744246	0.0
10	3.310304647336694E-4	0.0	0.0	8.715204560275682	0.0
11	3.310304647336694E-4	0.0	0.0	10.394853138334321	0.0
12	3.310304647336694E-4	0.0	0.0	11.832683961905014	0.0
13	3.310304647336694E-4	0.0	0.0	12.409008001006333	0.0
14	3.310304647336694E-4	0.0	0.0	12.645032722361439	0.0
15	3.310304647336694E-4	0.0	0.0	13.041110673415274	0.0
16	3.310304647336694E-4	0.0	0.0	13.858921436539804	0.0
17	3.310304647336694E-4	0.0	0.0	14.887102060002581	0.0
18	3.310304647336694E-4	0.0	0.0	15.947558153776892	0.0
19	4.965456971005042E-4	0.0	0.0	16.66920456689629	0.0
20	4.965456971005042E-4	0.0	0.0	17.358906540168892	0.0
21	4.965456971005042E-4	0.0	0.0	18.19988943582478	0.0
22	4.965456971005042E-4	0.0	0.0	19.13322983114136	0.0
23	4.965456971005042E-4	0.0	0.0	20.04091536544108	0.0
24	4.965456971005042E-4	0.0	0.0	20.742865465908828	0.0
25	4.965456971005042E-4	0.0	0.0	21.35874764554582	0.0
26	4.965456971005042E-4	0.0	0.0	21.927457983958263	0.0
27	4.965456971005042E-4	0.0	0.0	22.494844200511775	0.0
28	0.0011586066265678431	0.0	0.0	23.109236743057462	0.0
29	0.0011586066265678431	0.0	0.0	23.705422610042802	0.0
30	0.0011586066265678431	0.0	0.0	24.390324641576765	0.0
31	0.0011586066265678431	0.0	0.0	25.01961355503547	0.0
32	0.0011586066265678431	0.0	0.0	25.648074892332343	0.0
33	0.0013241218589346777	0.0	0.0	26.244922820247147	0.0
34	0.0014896370913015124	0.0	0.0	26.822736496439767	0.0
35	0.0014896370913015124	0.0	0.0	27.42488091179031	0.0
36	0.0014896370913015124	0.0	0.0	28.035301088759198	0.0
37	0.0014896370913015124	0.0	0.0	28.622714648429096	0.0
38	0.0016551523236683471	0.0	0.0	29.235617553883483	0.0
39	0.0016551523236683471	0.0	0.0	29.852989370611777	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGCA	20	7.030627E-4	45.0	10
ACAGACG	20	7.030627E-4	45.0	1
TATCGCG	20	7.030627E-4	45.0	32
AATACGA	20	7.030627E-4	45.0	27
GCTACGA	90	0.0	42.5	10
TATTGCG	60	3.6379788E-12	41.249996	1
TGTAACG	60	3.6379788E-12	41.249996	1
CGTTTTT	715	0.0	39.65035	1
TATAGCG	35	6.24337E-6	38.571426	1
TGGGCGC	70	0.0	38.571426	6
TACGTAG	35	6.24337E-6	38.571426	1
TAGTACG	35	6.24337E-6	38.571426	1
TAATCGT	35	6.24337E-6	38.571426	21
CCATCGA	100	0.0	38.25	31
TAGGGTC	195	0.0	38.076927	5
TACGAGG	65	9.094947E-12	38.07692	2
CCGATAA	30	1.1392811E-4	37.499996	11
CGACCCG	60	1.546141E-10	37.499996	32
TACGGAG	30	1.1392811E-4	37.499996	19
ATCCGCT	30	1.1392811E-4	37.499996	1
>>END_MODULE