##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935909.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639266
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.956567375709017	33.0	31.0	34.0	30.0	34.0
2	32.088851902025134	33.0	31.0	34.0	30.0	34.0
3	32.14531196716234	33.0	31.0	34.0	30.0	34.0
4	35.73020307665354	37.0	35.0	37.0	33.0	37.0
5	35.782154846339395	37.0	35.0	37.0	35.0	37.0
6	35.81355648509384	37.0	35.0	37.0	35.0	37.0
7	36.02676507119102	37.0	35.0	37.0	35.0	37.0
8	35.76442357328561	37.0	35.0	37.0	35.0	37.0
9	37.54666132720964	39.0	37.0	39.0	35.0	39.0
10	37.0302143395707	39.0	37.0	39.0	33.0	39.0
11	37.077634349394465	39.0	37.0	39.0	34.0	39.0
12	37.05130884483142	39.0	37.0	39.0	34.0	39.0
13	37.033560364543085	39.0	37.0	39.0	33.0	39.0
14	38.05850772604831	40.0	37.0	41.0	33.0	41.0
15	38.2369889842413	40.0	38.0	41.0	33.0	41.0
16	38.38297359784503	40.0	38.0	41.0	34.0	41.0
17	38.39542068559879	40.0	38.0	41.0	34.0	41.0
18	38.39200426739417	40.0	38.0	41.0	34.0	41.0
19	38.34303873504926	40.0	37.0	41.0	34.0	41.0
20	38.175161826219444	40.0	37.0	41.0	34.0	41.0
21	38.17772570416697	40.0	37.0	41.0	34.0	41.0
22	38.21096695272391	40.0	37.0	41.0	34.0	41.0
23	38.23356787315453	40.0	37.0	41.0	34.0	41.0
24	38.13518629177838	40.0	37.0	41.0	34.0	41.0
25	38.067771475410865	40.0	37.0	41.0	34.0	41.0
26	37.896906764946046	40.0	36.0	41.0	34.0	41.0
27	37.85268417215995	40.0	36.0	41.0	33.0	41.0
28	37.851975546955416	40.0	36.0	41.0	33.0	41.0
29	37.79539815976448	40.0	36.0	41.0	33.0	41.0
30	37.685206158312816	40.0	36.0	41.0	33.0	41.0
31	37.69746083789846	40.0	36.0	41.0	33.0	41.0
32	37.58086774519527	40.0	36.0	41.0	33.0	41.0
33	37.546972621725544	40.0	36.0	41.0	33.0	41.0
34	37.41129357732149	40.0	36.0	41.0	33.0	41.0
35	37.32888187389913	40.0	36.0	41.0	32.0	41.0
36	37.24537516464195	40.0	36.0	41.0	32.0	41.0
37	37.25625482975788	40.0	36.0	41.0	32.0	41.0
38	37.223949029042686	40.0	36.0	41.0	32.0	41.0
39	37.190570122609365	40.0	36.0	41.0	32.0	41.0
40	37.078823212872265	40.0	35.0	41.0	32.0	41.0
41	36.94254191525906	39.0	35.0	41.0	31.0	41.0
42	36.94516836496857	39.0	35.0	41.0	31.0	41.0
43	36.933032571730706	39.0	35.0	41.0	31.0	41.0
44	36.90477359972218	39.0	35.0	41.0	31.0	41.0
45	36.81296518194304	39.0	35.0	41.0	31.0	41.0
46	36.751067630688944	39.0	35.0	41.0	31.0	41.0
47	36.697575031364096	39.0	35.0	41.0	31.0	41.0
48	36.64889263624219	39.0	35.0	41.0	31.0	41.0
49	36.648853528890946	39.0	35.0	41.0	31.0	41.0
50	36.536723367111655	39.0	35.0	41.0	31.0	41.0
51	35.53364952930392	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	5.0
10	15.0
11	12.0
12	4.0
13	13.0
14	12.0
15	15.0
16	33.0
17	56.0
18	102.0
19	182.0
20	384.0
21	603.0
22	976.0
23	1467.0
24	2279.0
25	3679.0
26	5116.0
27	5964.0
28	6460.0
29	7344.0
30	8926.0
31	11262.0
32	15475.0
33	21773.0
34	38122.0
35	43616.0
36	43990.0
37	67808.0
38	132289.0
39	221109.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06532491951707	22.609367618487454	26.225546173267468	11.099761288728011
2	32.34819308394315	24.929684982464266	25.651919545228434	17.070202388364155
3	30.227166781902998	24.42551301023361	28.696192195424125	16.65112801243927
4	27.055091307843682	25.799432474118756	29.42296321093254	17.72251300710502
5	24.80939077003939	28.88296890496288	27.169597632284525	19.138042692713206
6	23.703434876874415	34.060156491976734	26.896315461795243	15.340093169353603
7	78.28540857796285	5.267916641898678	10.921118908247896	5.525555871890575
8	79.15937966355163	4.795969127092635	9.954854473724554	6.089796735631177
9	74.17444381525061	5.706231834635347	12.509346656947187	7.609977693166852
10	38.03455838414681	22.23831706988953	21.599615809381383	18.127508736582268
11	28.601395976009986	24.845838821398292	25.452002765671878	21.100762436919844
12	25.98401917198787	21.495120966858867	30.344332406228393	22.176527454924866
13	24.348862601796437	22.82211160925186	32.90711534791464	19.921910441037063
14	20.486620592992587	26.12120776014992	31.458109769642057	21.934061877215434
15	19.98870579696089	24.941573617242273	35.06443327190872	20.005287313888115
16	22.480313359384045	25.148842578832596	30.601502348005365	21.76934171377799
17	22.327012542509692	25.255996721239672	28.784731238639317	23.632259497611326
18	23.424364818401102	25.127411750351186	30.548472779719237	20.89975065152847
19	21.905122437295272	28.151505007305254	28.43417294209296	21.509199613306514
20	21.72616719800521	28.70041578935842	29.234152919129127	20.339264093507243
21	20.722203276883175	28.74202601108146	31.03919182312214	19.49657888891322
22	19.43150425644411	25.158854060750922	30.376556863653004	25.033084819151963
23	19.404754828193585	27.380777328999194	30.11610190437158	23.098365938435645
24	20.63053564556851	26.37853413133187	29.488507131616572	23.502423091483042
25	20.252915061961687	28.03981441215394	27.04726983759499	24.660000688289383
26	18.89917499131817	27.849596255705762	29.004045264412625	24.247183488563444
27	20.632569227833173	26.851263793162783	28.752506781214706	23.763660197789342
28	17.55575926140292	26.463631727637633	29.56875541636815	26.411853594591296
29	19.192010837429176	26.506493384600464	28.958524307565238	25.342971470405125
30	21.326959356512	25.841981272271635	27.297400456148146	25.53365891506822
31	20.28983240153551	26.619904703206487	26.402311400887896	26.687951494370104
32	20.490531328116933	27.137216745454946	27.73211777257042	24.64013415385771
33	20.38337718570986	24.06071963783464	27.285042533155213	28.27086064330028
34	19.231900335697503	24.52140423548257	29.32409982698908	26.92259560183085
35	18.14612383577415	25.65207597463341	29.817791029086482	26.384009160505954
36	19.90204390660539	25.391933874161932	28.315286594312855	26.39073562491983
37	19.39317905222552	24.757612636993052	30.90904255818392	24.94016575259751
38	18.892135668094344	24.08027331345637	30.42786570848442	26.599725309964867
39	20.939796579201772	22.053886801425385	30.890114600182084	26.11620201919076
40	22.299324537829325	22.66192789855866	31.138211636470576	23.90053592714144
41	19.769391771187582	24.350896184061092	29.988611939317906	25.891100105433416
42	19.75062024259072	23.26809184283225	32.90883607136935	24.07245184320768
43	19.71182575015721	22.92754502820422	31.700888206161444	25.659741015477127
44	19.81381772220015	22.977133149580926	28.910500480238273	28.298548647980653
45	20.918991468340252	21.962375599515692	28.049356605857344	29.069276326286708
46	22.321224654525658	22.90220346459846	28.615161763647684	26.161410117228197
47	18.631993567622867	23.110411002618626	32.75835098378453	25.49924444597398
48	18.92623727837864	22.718868201969133	31.49002136825672	26.864873151395507
49	20.586578982770863	20.750673428588414	32.55202059862405	26.110726990016676
50	19.462320849223953	20.32330829419991	31.441997540929755	28.772373315646384
51	19.187943672899856	21.010971958464864	28.669129908363654	31.131954460271622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	230.5
2	342.0
3	1997.5
4	3653.0
5	2537.5
6	1422.0
7	1431.0
8	1440.0
9	1496.0
10	1552.0
11	1503.0
12	1454.0
13	1463.0
14	1472.0
15	1461.5
16	1451.0
17	1373.5
18	1296.0
19	1469.5
20	1643.0
21	1574.5
22	1506.0
23	1646.5
24	1787.0
25	2246.5
26	3094.0
27	3482.0
28	4087.0
29	4692.0
30	5389.5
31	6087.0
32	7456.5
33	8826.0
34	10825.5
35	12825.0
36	14165.0
37	15505.0
38	17817.5
39	20130.0
40	22498.5
41	24867.0
42	29423.0
43	33979.0
44	39899.0
45	45819.0
46	59023.5
47	72228.0
48	80078.0
49	87928.0
50	86361.5
51	84795.0
52	72158.5
53	59522.0
54	50359.5
55	41197.0
56	35413.5
57	29630.0
58	25774.0
59	21918.0
60	19300.5
61	16683.0
62	13616.0
63	10549.0
64	8728.5
65	6908.0
66	5569.0
67	4230.0
68	3381.5
69	2533.0
70	1855.0
71	1177.0
72	1033.5
73	890.0
74	677.5
75	384.0
76	303.0
77	238.5
78	174.0
79	102.5
80	31.0
81	33.0
82	35.0
83	19.5
84	4.0
85	5.5
86	7.0
87	5.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48578948859169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70774600967121	29.436304991375405
2	11.814603823630838	9.56647126589257
3	4.338312562529765	5.269200274268785
4	2.245506315877622	3.6364438399969794
5	1.390879182404927	2.815542089145519
6	0.9912132316498642	2.4078030140929845
7	0.7812562753739671	2.2140843971002155
8	0.5936101911275785	1.9226221789019056
9	0.4974388564847585	1.8125284344378934
>10	4.528883452841374	33.039381148952444
>50	0.06875200480727248	1.8959288938947525
>100	0.03593854776894561	2.8114721221801933
>500	0.002343818332757322	0.6020855245058219
>1k	0.003515727499135983	2.57013182525456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2719	0.425331552123842	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2226	0.34821185547174416	TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	2087	0.3264681681803819	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2080	0.32537316234556507	No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	1870	0.2925229873010609	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	1512	0.23652126032043	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	1356	0.2121182731445126	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1291	0.2019503618212137	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1110	0.1736366395209506	TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	874	0.13671929994712687	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	667	0.10433841311754419	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	646	0.10105339561309377	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.128588099476587E-4	0.0	0.0	0.3039423338641504	0.0
2	3.128588099476587E-4	0.0	0.0	1.4414969668338375	0.0
3	3.128588099476587E-4	0.0	0.0	2.138702824802195	0.0
4	3.128588099476587E-4	0.0	0.0	3.0503733969896727	0.0
5	3.128588099476587E-4	0.0	0.0	5.460324810016488	0.0
6	3.128588099476587E-4	0.0	0.0	7.086877762934366	0.0
7	3.128588099476587E-4	0.0	0.0	8.59689080914674	0.0
8	3.128588099476587E-4	0.0	0.0	10.844312070405747	0.0
9	3.128588099476587E-4	0.0	0.0	11.768653424396105	0.0
10	3.128588099476587E-4	0.0	0.0	13.849320939953008	0.0
11	3.128588099476587E-4	0.0	0.0	16.444328339063865	0.0
12	3.128588099476587E-4	0.0	0.0	18.60759058044695	0.0
13	3.128588099476587E-4	0.0	0.0	19.480466660200918	0.0
14	3.128588099476587E-4	0.0	0.0	19.879518072289155	0.0
15	3.128588099476587E-4	0.0	0.0	20.433903883516408	0.0
16	3.128588099476587E-4	0.0	0.0	21.567704210766724	0.0
17	3.128588099476587E-4	0.0	0.0	22.996061107582758	0.0
18	3.128588099476587E-4	0.0	0.0	24.43646306858178	0.0
19	4.692882149214881E-4	0.0	0.0	25.39287245059177	0.0
20	4.692882149214881E-4	0.0	0.0	26.282642906082913	0.0
21	4.692882149214881E-4	0.0	0.0	27.43411975609527	0.0
22	4.692882149214881E-4	0.0	0.0	28.633933292244542	0.0
23	4.692882149214881E-4	0.0	0.0	29.822796770045645	0.0
24	4.692882149214881E-4	0.0	0.0	30.74948456511061	0.0
25	4.692882149214881E-4	0.0	0.0	31.57339824110777	0.0
26	4.692882149214881E-4	0.0	0.0	32.36336673622561	0.0
27	4.692882149214881E-4	0.0	0.0	33.18415182412329	0.0
28	4.692882149214881E-4	0.0	0.0	33.95425378480945	0.0
29	4.692882149214881E-4	0.0	0.0	34.780670331286196	0.0
30	4.692882149214881E-4	0.0	0.0	35.66449646938833	0.0
31	4.692882149214881E-4	0.0	0.0	36.47292363429308	0.0
32	4.692882149214881E-4	0.0	0.0	37.251785641657776	0.0
33	4.692882149214881E-4	0.0	0.0	37.964165151908595	0.0
34	4.692882149214881E-4	0.0	0.0	38.68045539728376	0.0
35	6.257176198953174E-4	0.0	0.0	39.41770718292542	0.0
36	6.257176198953174E-4	0.0	0.0	40.12351665816733	0.0
37	6.257176198953174E-4	0.0	0.0	40.85247768534538	0.0
38	6.257176198953174E-4	0.0	0.0	41.61256816411322	0.0
39	6.257176198953174E-4	0.0	0.0	42.62748214358342	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAT	20	7.030936E-4	45.0	9
TTGCGAG	20	7.030936E-4	45.0	1
CGAATGC	20	7.030936E-4	45.0	45
ACGCTCG	20	7.030936E-4	45.0	1
ACCGTAT	20	7.030936E-4	45.0	45
CAGTACG	25	3.8888567E-5	45.0	1
TAGTACG	20	7.030936E-4	45.0	1
GCGTCGG	30	2.1639844E-6	44.999996	1
CGTTTTT	1735	0.0	42.276657	1
TACGGGT	145	0.0	41.89655	4
TCCGGGT	50	1.0804797E-9	40.5	4
GCGAGAC	45	1.925946E-8	40.0	21
ACACGAC	40	3.4550067E-7	39.375	26
TATTGCG	35	6.2439394E-6	38.571426	1
AGGGCGC	70	0.0	38.571426	6
ATTGACG	35	6.2439394E-6	38.571426	1
GCTACGG	100	0.0	38.25	2
CGAATAG	95	0.0	37.894737	26
CGTAAGG	90	0.0	37.5	2
GCTCGTA	90	0.0	37.5	32
>>END_MODULE