FastQCFastQC Report
Sat 14 Jan 2017
SRR2935908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785665
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC70790.9010201549006256No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC66270.8434892734180599No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG60730.7729757593885435No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.34556713102912817No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC23380.29758230288990856No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT15310.1948667689155047No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT13430.17093799520151717No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT12220.15553702914091883No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC11080.1410270280590328No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT10460.1331356239618667No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC9930.12638974626590213No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA8940.11378895585268531No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG8490.10806132384667765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA551.8189894E-1245.041
TATTGCG253.8896753E-545.01
CCCGTCG302.164621E-644.99999640
ACGGGTC1000.042.755
CGTTTTT19300.042.2020761
TCGAATG1400.041.78571344
CGATGAA8300.041.2048219
GCGCGAC1150.041.0869569
TTTAGCG1000.040.51
GTTTGCG451.9270374E-840.0000041
CCGATGA8550.039.7368418
CTTAACG403.4563527E-739.3750041
AAGTACG403.4563527E-739.3750041
TAGCACG403.4563527E-739.3750041
CGCGTGG403.4563527E-739.3750042
TACGGCT7800.038.9423077
TACGCGG356.2457657E-638.5714262
GCGGAAT356.2457657E-638.57142622
TTACCGG356.2457657E-638.5714262
CGGGTCC1350.038.3333326
Produced by FastQC (version 0.11.5)