##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935908.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 785665 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17919087651862 33.0 31.0 34.0 30.0 34.0 2 32.297852137997744 34.0 31.0 34.0 30.0 34.0 3 32.352370285045154 34.0 31.0 34.0 30.0 34.0 4 35.89938714337536 37.0 35.0 37.0 35.0 37.0 5 35.91410333920946 37.0 35.0 37.0 35.0 37.0 6 35.92493110931504 37.0 35.0 37.0 35.0 37.0 7 36.08370106852157 37.0 35.0 37.0 35.0 37.0 8 35.74173089039221 37.0 35.0 37.0 35.0 37.0 9 37.57300376114502 39.0 38.0 39.0 35.0 39.0 10 37.19321593809066 39.0 37.0 39.0 34.0 39.0 11 37.19298937842465 39.0 37.0 39.0 34.0 39.0 12 37.15615052216912 39.0 37.0 39.0 34.0 39.0 13 37.140664278031984 39.0 37.0 39.0 34.0 39.0 14 38.259641195675 40.0 38.0 41.0 33.0 41.0 15 38.384375019887614 40.0 38.0 41.0 34.0 41.0 16 38.558505215327145 40.0 38.0 41.0 34.0 41.0 17 38.50421108233153 40.0 38.0 41.0 34.0 41.0 18 38.4898194523111 40.0 38.0 41.0 34.0 41.0 19 38.460928003665686 40.0 37.0 41.0 34.0 41.0 20 38.204596106483045 40.0 37.0 41.0 34.0 41.0 21 38.26599504878033 40.0 37.0 41.0 34.0 41.0 22 38.27183596061935 40.0 37.0 41.0 34.0 41.0 23 38.23197418747176 40.0 37.0 41.0 34.0 41.0 24 38.170422508320975 40.0 37.0 41.0 34.0 41.0 25 38.17168131455519 40.0 37.0 41.0 34.0 41.0 26 37.99687398573183 40.0 37.0 41.0 34.0 41.0 27 37.92657812171854 40.0 36.0 41.0 34.0 41.0 28 37.93006688601376 40.0 36.0 41.0 34.0 41.0 29 37.91939185276167 40.0 36.0 41.0 34.0 41.0 30 37.835180388588014 40.0 36.0 41.0 33.0 41.0 31 37.85380664787155 40.0 37.0 41.0 33.0 41.0 32 37.688918304875486 40.0 36.0 41.0 33.0 41.0 33 37.60664278031986 40.0 36.0 41.0 33.0 41.0 34 37.47752413560487 40.0 36.0 41.0 33.0 41.0 35 37.40607256273348 40.0 36.0 41.0 33.0 41.0 36 37.32453272068884 40.0 36.0 41.0 32.0 41.0 37 37.24079983198946 40.0 36.0 41.0 32.0 41.0 38 37.146386818809546 40.0 36.0 41.0 32.0 41.0 39 37.063300516123284 40.0 35.0 41.0 32.0 41.0 40 37.00535469952206 40.0 35.0 41.0 31.0 41.0 41 36.97120019346668 40.0 35.0 41.0 31.0 41.0 42 36.91699515696894 40.0 35.0 41.0 31.0 41.0 43 36.854805801454816 40.0 35.0 41.0 31.0 41.0 44 36.85737687182196 39.0 35.0 41.0 31.0 41.0 45 36.773512883989994 39.0 35.0 41.0 31.0 41.0 46 36.72471345929881 39.0 35.0 41.0 31.0 41.0 47 36.67733066892378 39.0 35.0 41.0 31.0 41.0 48 36.59805260511796 39.0 35.0 41.0 31.0 41.0 49 36.59674161379214 39.0 35.0 41.0 31.0 41.0 50 36.45637008139602 39.0 35.0 41.0 31.0 41.0 51 35.53710932776692 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 6.0 9 15.0 10 16.0 11 16.0 12 14.0 13 14.0 14 18.0 15 21.0 16 46.0 17 77.0 18 156.0 19 322.0 20 806.0 21 1477.0 22 2356.0 23 3071.0 24 3889.0 25 4895.0 26 6423.0 27 7231.0 28 7697.0 29 8254.0 30 9609.0 31 12270.0 32 16853.0 33 23862.0 34 43763.0 35 50878.0 36 50780.0 37 77051.0 38 151043.0 39 302452.0 40 277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.709214487090556 20.416335206481133 24.92550896374409 10.948941342684224 2 30.785003786601163 28.14036516836056 24.511973932910337 16.562657112127944 3 29.656151158572676 27.809435319124564 26.587285929753772 15.947127592548988 4 26.660345058008183 24.313797865502472 31.13209828616522 17.89375879032412 5 28.093907708756277 27.993610508295518 25.22589144228138 18.68659034066682 6 23.91490011646185 36.365499290410035 25.26165732214112 14.457943270986998 7 77.80109843253804 5.233782846378546 12.525312951448772 4.439805769634641 8 79.16656590277027 7.645625043752744 8.605576167959626 4.582232885517365 9 74.12981359739838 5.9816843056519 9.9580610056449 9.930441091304818 10 40.290072740926476 24.144005396702156 18.947515798718285 16.618406063653087 11 30.14707286184315 26.02495974747507 24.85372264260213 18.974244748079652 12 25.435904615834993 22.760718626895688 29.21308700273017 22.590289754539146 13 25.244219864700607 22.61816423030172 32.405159960033856 19.73245594496382 14 20.390497222098478 27.763486982365258 30.02450153691459 21.821514258621676 15 19.38027021694997 25.01931484793137 34.36973773809448 21.23067719702418 16 22.752572661376032 26.087200015273687 28.494714668465566 22.665512654884715 17 23.163180235851158 27.11346439003901 28.607612659339544 21.115742714770292 18 23.856987392845554 25.010150636721757 29.2679449892766 21.864916981156092 19 22.829832053101512 27.45941336320187 27.01418543526821 22.696569148428402 20 24.122494956501818 27.50676178778487 28.99123672303081 19.379506532682505 21 22.538359224351346 29.607911769010965 29.619494313734222 18.234234692903463 22 21.934539530206894 23.754780981716124 31.03001915574704 23.280660332329937 23 21.734581532841606 26.71813050091324 30.476857184677947 21.070430781567207 24 21.912519967161575 26.072180891346818 29.231033583015666 22.78426555847594 25 21.217440003054737 30.088396453959387 26.684528393144664 22.009635149841216 26 20.550870918266693 26.093436770124672 29.79094143178072 23.564750879827915 27 22.339419472676013 26.353343982486173 28.9760903183927 22.331146226445114 28 19.443783291861035 27.03009552417379 30.571808595266432 22.95431258869875 29 20.500595037325066 25.294495745642227 31.74610043720924 22.45880877982346 30 22.060801995761555 26.74295023960594 31.22972259168984 19.966525172942667 31 23.553550177238392 25.174597315649798 28.186313505119863 23.085539001991943 32 23.70832352211184 26.82428261409125 29.199595247338245 20.26779861645867 33 23.349010074268296 26.019741238314037 27.42415660618712 23.20709208123055 34 21.25562421642812 25.330007064079474 29.82123424105694 23.593134478435466 35 20.69978935042289 27.4186835356036 29.76828546517918 22.113241648794332 36 23.654611061966616 28.157675345089828 27.85563821730636 20.3320753756372 37 21.33861124015961 26.532173381784858 30.741346502644255 21.387868875411275 38 22.5216854511783 28.4454570332139 26.654744706713423 22.378112808894375 39 22.350747455976784 25.20132626501117 28.454366683001027 23.99355959601102 40 21.843151979533264 26.56208434892734 29.118008311430444 22.476755360108953 41 21.497839409926623 25.9072250895738 27.379990199385233 25.214945301114344 42 22.21710270916994 25.839257189769178 30.154327862384093 21.78931223867679 43 23.70857808353433 24.32258023457835 29.3506774515856 22.61816423030172 44 22.603526948508588 24.259321721089776 29.277491042619946 23.85966028778169 45 21.760928640069242 23.186599886720167 28.876047679354432 26.176423793856163 46 23.162161990161202 25.71999516333297 28.343250622084476 22.77459222442135 47 19.908612449326366 25.582786556611282 32.40834197781497 22.100259016247385 48 21.448581774674956 26.014650009864255 29.034003042008997 23.502765173451788 49 22.341201402633438 23.63208237607632 31.619583410232092 22.407132811058148 50 21.045865604296996 24.041417143439 30.693870797350016 24.21884645491399 51 21.04344727078335 23.429196922352403 28.547790725054572 26.979565081809675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 155.0 1 376.5 2 598.0 3 2721.0 4 4844.0 5 3344.0 6 1844.0 7 1826.5 8 1809.0 9 1792.0 10 1775.0 11 1721.5 12 1668.0 13 1660.5 14 1653.0 15 1699.0 16 1745.0 17 1689.5 18 1634.0 19 1527.5 20 1421.0 21 1661.0 22 1901.0 23 2303.5 24 2706.0 25 3217.0 26 4821.5 27 5915.0 28 7204.5 29 8494.0 30 10679.5 31 12865.0 32 14196.0 33 15527.0 34 18166.5 35 20806.0 36 22956.0 37 25106.0 38 27634.0 39 30162.0 40 33887.0 41 37612.0 42 42534.0 43 47456.0 44 67229.0 45 87002.0 46 84298.0 47 81594.0 48 80615.0 49 79636.0 50 71210.5 51 62785.0 52 57695.5 53 52606.0 54 48360.5 55 44115.0 56 40793.5 57 37472.0 58 34668.0 59 31864.0 60 27847.5 61 23831.0 62 21084.0 63 18337.0 64 15666.0 65 12995.0 66 10587.5 67 8180.0 68 6872.5 69 5565.0 70 4624.0 71 3683.0 72 2829.0 73 1975.0 74 1613.0 75 980.0 76 709.0 77 506.5 78 304.0 79 244.5 80 185.0 81 144.0 82 103.0 83 67.5 84 32.0 85 21.5 86 11.0 87 6.5 88 2.0 89 2.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 785665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.443935716579205 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48247292447742 23.19172756423859 2 10.331134752078427 6.703653435514998 3 3.7321398626702056 3.632559173692647 4 2.1060259843885607 2.7331108661979155 5 1.3997559546051581 2.2706796105054354 6 1.0949157854253608 2.131402641444494 7 0.8709489462735189 1.977990813772431 8 0.7559331841037605 1.962035810487315 9 0.6957145445201295 2.0314546163550236 >10 7.426512812199777 45.6122860641753 >50 0.06989492006438203 1.537116975912995 >100 0.0285933758841644 1.7186590996420097 >500 0.0019856511030669705 0.49257318674049033 >1k 0.0027799115442937584 1.4563313444226424 >5k 0.0011913906618401821 2.548418796897766 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC 7079 0.9010201549006256 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC 6627 0.8434892734180599 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG 6073 0.7729757593885435 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2715 0.34556713102912817 No Hit GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC 2338 0.29758230288990856 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT 1531 0.1948667689155047 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT 1343 0.17093799520151717 No Hit GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT 1222 0.15553702914091883 No Hit CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC 1108 0.1410270280590328 No Hit CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT 1046 0.1331356239618667 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC 993 0.12638974626590213 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA 894 0.11378895585268531 No Hit CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG 849 0.10806132384667765 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.818421337338433E-4 0.0 0.0 0.13911781739036358 0.0 2 3.818421337338433E-4 0.0 0.0 0.7094626844774808 0.0 3 3.818421337338433E-4 0.0 0.0 1.0592300789776814 0.0 4 3.818421337338433E-4 0.0 0.0 1.6197743312989632 0.0 5 3.818421337338433E-4 0.0 0.0 3.5695875468552116 0.0 6 3.818421337338433E-4 0.0 0.0 4.5175742842051 0.0 7 3.818421337338433E-4 0.0 0.0 5.445323388467095 0.0 8 3.818421337338433E-4 0.0 0.0 6.760133135623962 0.0 9 3.818421337338433E-4 0.0 0.0 7.303494491927221 0.0 10 3.818421337338433E-4 0.0 0.0 9.118517434275423 0.0 11 3.818421337338433E-4 0.0 0.0 10.403670775712294 0.0 12 3.818421337338433E-4 0.0 0.0 12.30702653166426 0.0 13 3.818421337338433E-4 0.0 0.0 12.812585516727868 0.0 14 3.818421337338433E-4 0.0 0.0 13.06091018436611 0.0 15 3.818421337338433E-4 0.0 0.0 13.482845742142008 0.0 16 3.818421337338433E-4 0.0 0.0 14.104230174438214 0.0 17 3.818421337338433E-4 0.0 0.0 14.809619876155868 0.0 18 3.818421337338433E-4 0.0 0.0 15.555866686183043 0.0 19 3.818421337338433E-4 0.0 0.0 16.259983580788248 0.0 20 3.818421337338433E-4 0.0 0.0 16.812381867589878 0.0 21 3.818421337338433E-4 0.0 0.0 17.45133103803784 0.0 22 3.818421337338433E-4 0.0 0.0 18.164993985986392 0.0 23 3.818421337338433E-4 0.0 0.0 18.82112605245238 0.0 24 3.818421337338433E-4 0.0 0.0 19.340049512196675 0.0 25 3.818421337338433E-4 0.0 0.0 19.830843934755908 0.0 26 3.818421337338433E-4 0.0 0.0 20.2812903718506 0.0 27 3.818421337338433E-4 0.0 0.0 20.805559621467165 0.0 28 5.091228449784577E-4 0.0 0.0 21.275607288093525 0.0 29 5.091228449784577E-4 0.0 0.0 21.79643995850649 0.0 30 5.091228449784577E-4 0.0 0.0 22.363475527101244 0.0 31 6.364035562230722E-4 0.0 0.0 22.851087931879363 0.0 32 6.364035562230722E-4 0.0 0.0 23.348119109289584 0.0 33 6.364035562230722E-4 0.0 0.0 23.846804935945983 0.0 34 7.636842674676866E-4 0.0 0.0 24.34905462251723 0.0 35 7.636842674676866E-4 0.0 0.0 24.871796503598862 0.0 36 7.636842674676866E-4 0.0 0.0 25.350371977878613 0.0 37 7.636842674676866E-4 0.0 0.0 25.841675523282824 0.0 38 7.636842674676866E-4 0.0 0.0 26.398274073555523 0.0 39 7.636842674676866E-4 0.0 0.0 27.188432728962088 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCGA 55 1.8189894E-12 45.0 41 TATTGCG 25 3.8896753E-5 45.0 1 CCCGTCG 30 2.164621E-6 44.999996 40 ACGGGTC 100 0.0 42.75 5 CGTTTTT 1930 0.0 42.202076 1 TCGAATG 140 0.0 41.785713 44 CGATGAA 830 0.0 41.20482 19 GCGCGAC 115 0.0 41.086956 9 TTTAGCG 100 0.0 40.5 1 GTTTGCG 45 1.9270374E-8 40.000004 1 CCGATGA 855 0.0 39.73684 18 CTTAACG 40 3.4563527E-7 39.375004 1 AAGTACG 40 3.4563527E-7 39.375004 1 TAGCACG 40 3.4563527E-7 39.375004 1 CGCGTGG 40 3.4563527E-7 39.375004 2 TACGGCT 780 0.0 38.942307 7 TACGCGG 35 6.2457657E-6 38.571426 2 GCGGAAT 35 6.2457657E-6 38.571426 22 TTACCGG 35 6.2457657E-6 38.571426 2 CGGGTCC 135 0.0 38.333332 6 >>END_MODULE