##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935906.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	249018
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97797347982877	33.0	31.0	34.0	30.0	34.0
2	32.10096057313126	33.0	31.0	34.0	30.0	34.0
3	32.16495996273362	34.0	31.0	34.0	30.0	34.0
4	35.73987021018561	37.0	35.0	37.0	33.0	37.0
5	35.77762250118465	37.0	35.0	37.0	35.0	37.0
6	35.809812945248936	37.0	35.0	37.0	35.0	37.0
7	35.98299721305287	37.0	35.0	37.0	35.0	37.0
8	35.682761888698806	37.0	35.0	37.0	35.0	37.0
9	37.42977616075947	39.0	37.0	39.0	34.0	39.0
10	37.1433952565678	39.0	37.0	39.0	33.0	39.0
11	37.08578897910994	39.0	37.0	39.0	34.0	39.0
12	36.98786031531857	39.0	37.0	39.0	33.0	39.0
13	36.953212217590696	39.0	37.0	39.0	33.0	39.0
14	38.06373836429495	40.0	37.0	41.0	33.0	41.0
15	38.229332819314266	40.0	37.0	41.0	34.0	41.0
16	38.35240022809596	40.0	37.0	41.0	34.0	41.0
17	38.32716912030455	40.0	37.0	41.0	34.0	41.0
18	38.313503441518286	40.0	37.0	41.0	34.0	41.0
19	38.317559373218	40.0	37.0	41.0	34.0	41.0
20	38.173272614831056	40.0	37.0	41.0	34.0	41.0
21	38.150145772594755	40.0	37.0	41.0	34.0	41.0
22	38.16728911163048	40.0	37.0	41.0	34.0	41.0
23	38.17443718927949	40.0	37.0	41.0	34.0	41.0
24	38.11968612710728	40.0	36.0	41.0	34.0	41.0
25	38.03500550161033	40.0	36.0	41.0	34.0	41.0
26	37.90794641351228	40.0	36.0	41.0	34.0	41.0
27	37.84136488125356	40.0	36.0	41.0	33.0	41.0
28	37.72660209302139	40.0	36.0	41.0	33.0	41.0
29	37.77053064437109	40.0	36.0	41.0	33.0	41.0
30	37.634448112184664	40.0	36.0	41.0	33.0	41.0
31	37.57665710912464	40.0	35.0	41.0	33.0	41.0
32	37.516127348223826	40.0	35.0	41.0	33.0	41.0
33	37.49463492598929	40.0	35.0	41.0	33.0	41.0
34	37.40140873350521	40.0	35.0	41.0	33.0	41.0
35	37.45332064348762	40.0	35.0	41.0	33.0	41.0
36	37.33884297520661	40.0	35.0	41.0	33.0	41.0
37	37.23570183681501	40.0	35.0	41.0	32.0	41.0
38	37.14203792496928	39.0	35.0	41.0	32.0	41.0
39	37.21450658185352	39.0	35.0	41.0	32.0	41.0
40	37.26689636893718	39.0	35.0	41.0	33.0	41.0
41	37.1872635713081	39.0	35.0	41.0	32.0	41.0
42	37.13673710334193	39.0	35.0	41.0	32.0	41.0
43	37.01635223156559	39.0	35.0	41.0	32.0	41.0
44	36.92331478045764	39.0	35.0	41.0	32.0	41.0
45	36.83698367186308	39.0	35.0	41.0	31.0	41.0
46	36.82180003051988	39.0	35.0	41.0	31.0	41.0
47	36.75851143290846	39.0	35.0	41.0	31.0	41.0
48	36.72811603980435	39.0	35.0	41.0	31.0	41.0
49	36.71107711089158	39.0	35.0	41.0	31.0	41.0
50	36.59853102988539	39.0	35.0	41.0	31.0	41.0
51	35.602868065762316	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	3.0
11	10.0
12	6.0
13	4.0
14	13.0
15	4.0
16	16.0
17	21.0
18	54.0
19	73.0
20	140.0
21	210.0
22	421.0
23	564.0
24	803.0
25	1098.0
26	1521.0
27	1828.0
28	2103.0
29	2660.0
30	3310.0
31	4565.0
32	5837.0
33	8106.0
34	15854.0
35	21568.0
36	18288.0
37	26831.0
38	49329.0
39	83714.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.764523046526755	21.847416652611457	24.799010513296228	12.589049787565557
2	31.446321149475136	24.263306267016844	25.560802833530104	18.729569749977916
3	31.073255748580426	23.661341750395554	27.47833489948518	17.787067601538844
4	28.790288252254857	24.727128159410164	26.959095326442267	19.523488261892716
5	25.30700591925082	28.689492325855966	25.602566882715305	20.400934872177913
6	25.571645423222417	32.74582560296846	25.421053899718093	16.26147507409103
7	76.15192476045908	6.207181810150271	11.120079672955368	6.5208137564352775
8	75.17488695596303	5.596784168212739	10.981535471331389	8.246793404492848
9	68.78418427583549	7.5520645093928955	13.950798737440667	9.712952477330957
10	40.62959304146688	22.352199439397953	21.076789629665328	15.94141788946984
11	28.009220217012427	23.844862620372826	25.84391489771824	22.302002264896515
12	25.84993855865841	20.5989125284116	31.812158157241644	21.738990755688345
13	23.222016079158937	24.088620099751825	33.23012794255837	19.45923587853087
14	18.554080427920873	28.147764418636406	31.247540338449433	22.050614814993295
15	16.750596342433077	24.701427206065425	37.74907837987615	20.798898071625345
16	18.930358447983682	25.11906769791742	32.665911701162166	23.284662152936736
17	18.423166196821114	25.379289850532892	30.531527841360866	25.666016111285128
18	19.79977351034865	24.599828124874506	33.407223574199456	22.193174790577388
19	21.45788657848027	25.756772602783734	29.097896537599695	23.6874442811363
20	22.61964998514164	26.959095326442267	29.756483467058608	20.66477122135749
21	21.491619079745238	26.960701636026315	31.220634652916658	20.327044631311793
22	19.696969696969695	25.103807756868978	28.326064782465526	26.873157763695797
23	18.465331823402327	26.670762756105987	30.09862740846043	24.76527801203126
24	21.373153747921837	23.67860957842405	30.459243910078786	24.488992763575325
25	19.089383096804248	26.246697025917804	29.286637913725112	25.377281963552832
26	20.137500100394348	26.44949361090363	29.348480832710887	24.064525455991134
27	23.084676609722994	27.18357709081271	28.093149892778836	21.63859640668546
28	18.10029797042784	25.97603386100603	31.31701322795942	24.606654940606703
29	22.312041699796804	23.99585572127316	29.33081142728638	24.36129115164366
30	23.821169554008144	25.16163490189464	29.479796641206658	21.537398902890555
31	21.909259571597232	26.501698672385128	28.173465371981145	23.415576384036495
32	24.442811363033996	27.145025660795607	25.87523793460714	22.53692504156326
33	23.27783533720454	25.364029909484454	28.795910335799018	22.562224417511985
34	20.25917805138584	23.805909612959706	32.8586688512477	23.07624348440675
35	21.904842220241107	24.67090732396855	27.812447292966773	25.61180316282357
36	24.329968114754756	25.04316957007124	30.14239934462569	20.484462970548314
37	20.849095246126787	25.52265298090901	33.10082002104265	20.527431751921547
38	18.549663076564745	27.20686857978138	29.253306989856153	24.990161353797717
39	19.934301938012513	26.41776899661872	32.03824623119614	21.60968283417263
40	22.186749552241203	22.129725562007565	32.36794127332161	23.315583612429624
41	19.74435582969906	24.80021524548426	28.54733392766788	26.9080949971488
42	22.458215871945	23.488261892714583	29.303102586961582	24.750419648378834
43	22.938904014970802	24.14524251258945	29.62356134897879	23.292292123460957
44	20.78163024359685	23.923571789991087	29.66291593378792	25.631882032624148
45	21.14104201302717	21.830550401978975	30.092603747520258	26.9358038374736
46	23.118810688383974	23.489065047506603	30.438361885486188	22.95376237862323
47	18.690215165168784	23.23607128801934	33.841328739287924	24.232384807523953
48	19.24961247781285	22.57105912022424	31.149153876426606	27.030174525536303
49	21.477965448280848	20.697298990434426	34.47300998321406	23.351725578070663
50	20.440691034383057	20.353147162052544	33.22852163297432	25.977640170590078
51	18.99822502790963	20.597707796223567	29.355709225839092	31.04835795002771
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	182.0
1	280.0
2	378.0
3	655.0
4	932.0
5	636.5
6	341.0
7	358.5
8	376.0
9	390.0
10	404.0
11	428.0
12	452.0
13	423.0
14	394.0
15	453.0
16	512.0
17	513.5
18	515.0
19	562.0
20	609.0
21	595.5
22	582.0
23	701.0
24	820.0
25	994.5
26	1419.0
27	1669.0
28	1771.0
29	1873.0
30	2168.0
31	2463.0
32	3098.0
33	3733.0
34	4460.0
35	5187.0
36	6325.0
37	7463.0
38	7970.5
39	8478.0
40	9923.5
41	11369.0
42	13939.5
43	16510.0
44	21859.0
45	27208.0
46	30218.5
47	33229.0
48	33006.5
49	32784.0
50	27711.0
51	22638.0
52	18788.0
53	14938.0
54	13212.0
55	11486.0
56	10602.5
57	9719.0
58	8737.0
59	7755.0
60	7131.5
61	6508.0
62	5906.5
63	5305.0
64	4671.0
65	4037.0
66	3152.0
67	2267.0
68	2051.5
69	1836.0
70	1456.0
71	1076.0
72	890.0
73	704.0
74	645.0
75	421.5
76	257.0
77	229.0
78	201.0
79	119.0
80	37.0
81	30.5
82	24.0
83	14.0
84	4.0
85	3.0
86	2.0
87	3.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	249018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.616156261796334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.62804923615633	26.56755736533102
2	12.536430698935636	9.431446722726871
3	4.783764452178369	5.398404934583042
4	2.381740346532011	3.5836766820069235
5	1.4860522466932136	2.7949786762402717
6	0.9565393771818386	2.158880080958003
7	0.7024586051179127	1.8496654860291224
8	0.5626074238558358	1.6930503015846243
9	0.4654588933608054	1.5757897019492568
>10	5.373061032763609	37.75349573123228
>50	0.07793233764985961	2.0380052847585315
>100	0.03736481942116557	2.3885823514766003
>500	0.006405397615056954	1.5083246994193191
>1k	0.002135132538352318	1.258141981704134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1597	0.6413191014304187	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1536	0.6168228802737151	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	934	0.3750732878747721	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	620	0.24897798552715064	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	609	0.24456063417102378	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	550	0.22086756780634334	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.21283601988611264	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	513	0.20600920415391658	No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	445	0.17870194122513233	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	388	0.15581202965247493	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	335	0.13452842766386366	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	331	0.13292211807981752	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	257	0.10320539077496406	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.015773960115333E-4	0.0	0.0	0.3919395385072565	0.0
2	4.015773960115333E-4	0.0	0.0	2.146832759077657	0.0
3	4.015773960115333E-4	0.0	0.0	2.9724758852773694	0.0
4	4.015773960115333E-4	0.0	0.0	4.024207085431575	0.0
5	4.015773960115333E-4	0.0	0.0	6.902312282646235	0.0
6	4.015773960115333E-4	0.0	0.0	8.257234416789148	0.0
7	4.015773960115333E-4	0.0	0.0	9.82177995165008	0.0
8	4.015773960115333E-4	0.0	0.0	12.36456802319511	0.0
9	4.015773960115333E-4	0.0	0.0	13.314700142158399	0.0
10	8.031547920230666E-4	0.0	0.0	15.34226441462063	0.0
11	8.031547920230666E-4	0.0	0.0	18.015163562473397	0.0
12	8.031547920230666E-4	0.0	0.0	20.29692632661093	0.0
13	8.031547920230666E-4	0.0	0.0	21.169152430747978	0.0
14	8.031547920230666E-4	0.0	0.0	21.536997325494543	0.0
15	8.031547920230666E-4	0.0	0.0	22.187151129637215	0.0
16	8.031547920230666E-4	0.0	0.0	23.48745873792256	0.0
17	0.0012047321880345999	0.0	0.0	25.05883108851569	0.0
18	0.0012047321880345999	0.0	0.0	26.6852195423624	0.0
19	0.0012047321880345999	0.0	0.0	27.707234015211753	0.0
20	0.0016063095840461333	0.0	0.0	28.68868917106394	0.0
21	0.0016063095840461333	0.0	0.0	29.81792480864837	0.0
22	0.0016063095840461333	0.0	0.0	30.97607401874563	0.0
23	0.0016063095840461333	0.0	0.0	32.04145885036423	0.0
24	0.0016063095840461333	0.0	0.0	32.92292123460955	0.0
25	0.0016063095840461333	0.0	0.0	33.70358769245597	0.0
26	0.0016063095840461333	0.0	0.0	34.40273393891205	0.0
27	0.0016063095840461333	0.0	0.0	35.074572922439344	0.0
28	0.0016063095840461333	0.0	0.0	35.75163241211479	0.0
29	0.0016063095840461333	0.0	0.0	36.457605474303065	0.0
30	0.0016063095840461333	0.0	0.0	37.24550032527769	0.0
31	0.0016063095840461333	0.0	0.0	37.99926109759134	0.0
32	0.0016063095840461333	0.0	0.0	38.68354898039499	0.0
33	0.0016063095840461333	0.0	0.0	39.355789541318295	0.0
34	0.0016063095840461333	0.0	0.0	39.98988024962051	0.0
35	0.0016063095840461333	0.0	0.0	40.67617601940422	0.0
36	0.0016063095840461333	0.0	0.0	41.33516452625915	0.0
37	0.0016063095840461333	0.0	0.0	41.98049940164968	0.0
38	0.0016063095840461333	0.0	0.0	42.60535382984362	0.0
39	0.0016063095840461333	0.0	0.0	43.23944453814584	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAT	20	7.0226315E-4	45.000004	18
CGCATGA	20	7.0226315E-4	45.000004	20
CGCGCCT	40	6.7775545E-9	45.000004	17
TAGCCGT	30	2.158631E-6	45.000004	44
CGTTAGG	20	7.0226315E-4	45.000004	2
ACGATGC	20	7.0226315E-4	45.000004	43
GTTAAAC	20	7.0226315E-4	45.000004	27
TAATTCG	20	7.0226315E-4	45.000004	4
AATTCGG	20	7.0226315E-4	45.000004	5
TACTCCG	20	7.0226315E-4	45.000004	34
TGCTCAA	20	7.0226315E-4	45.000004	33
TTTGCGC	40	6.7775545E-9	45.000004	13
CCGAATT	20	7.0226315E-4	45.000004	20
CGATGCC	20	7.0226315E-4	45.000004	44
CAGTCCG	20	7.0226315E-4	45.000004	15
GCGTGGA	20	7.0226315E-4	45.000004	3
CGCACGA	20	7.0226315E-4	45.000004	40
ACTCCGC	20	7.0226315E-4	45.000004	35
ATGCACG	20	7.0226315E-4	45.000004	1
CGCCATA	20	7.0226315E-4	45.000004	39
>>END_MODULE