##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935905.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933166
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938319655881163	33.0	31.0	34.0	30.0	34.0
2	31.989498117162434	33.0	31.0	34.0	30.0	34.0
3	31.98800320628913	33.0	31.0	34.0	30.0	34.0
4	35.70738218066239	37.0	35.0	37.0	33.0	37.0
5	33.61598579459603	37.0	35.0	37.0	32.0	37.0
6	34.59359106525527	37.0	35.0	37.0	30.0	37.0
7	35.710699918342506	37.0	35.0	37.0	32.0	37.0
8	35.733871572689104	37.0	35.0	37.0	35.0	37.0
9	37.63843839145447	39.0	37.0	39.0	35.0	39.0
10	37.14409547711768	39.0	37.0	39.0	34.0	39.0
11	37.1418900817218	39.0	37.0	39.0	34.0	39.0
12	37.22325824129898	39.0	37.0	39.0	34.0	39.0
13	37.22645274259885	39.0	37.0	39.0	34.0	39.0
14	38.342763238266286	40.0	38.0	41.0	34.0	41.0
15	38.42895904908666	40.0	38.0	41.0	34.0	41.0
16	38.41330910041729	40.0	38.0	41.0	34.0	41.0
17	38.385589487829606	40.0	38.0	41.0	34.0	41.0
18	38.31886502508664	40.0	38.0	41.0	34.0	41.0
19	38.205724383443034	40.0	37.0	41.0	34.0	41.0
20	38.19365579114541	40.0	37.0	41.0	34.0	41.0
21	38.127830418167825	40.0	37.0	41.0	34.0	41.0
22	38.21036021458133	40.0	37.0	41.0	34.0	41.0
23	38.20005658157284	40.0	37.0	41.0	34.0	41.0
24	38.15050376888999	40.0	37.0	41.0	34.0	41.0
25	37.969961400222466	40.0	36.0	41.0	34.0	41.0
26	38.020464740464185	40.0	37.0	41.0	34.0	41.0
27	38.02502448653294	40.0	37.0	41.0	34.0	41.0
28	37.92937698115877	40.0	36.0	41.0	34.0	41.0
29	37.95883154765605	40.0	37.0	41.0	34.0	41.0
30	37.80622418733645	40.0	36.0	41.0	33.0	41.0
31	37.744290940732945	40.0	36.0	41.0	33.0	41.0
32	37.61967431303755	40.0	36.0	41.0	33.0	41.0
33	37.48613429979232	40.0	36.0	41.0	33.0	41.0
34	37.32545120589477	40.0	36.0	41.0	33.0	41.0
35	37.21071599265297	40.0	36.0	41.0	32.0	41.0
36	37.090344054541205	40.0	36.0	41.0	32.0	41.0
37	37.03713272879638	40.0	36.0	41.0	31.0	41.0
38	36.92643538234355	40.0	36.0	41.0	31.0	41.0
39	36.86145444647576	40.0	36.0	41.0	31.0	41.0
40	36.791761594400135	40.0	35.0	41.0	31.0	41.0
41	36.65827623381049	40.0	35.0	41.0	30.0	41.0
42	36.69354648583425	40.0	35.0	41.0	30.0	41.0
43	36.633721117143146	39.0	35.0	41.0	30.0	41.0
44	36.50661940104976	39.0	35.0	41.0	30.0	41.0
45	36.47683263213619	39.0	35.0	41.0	30.0	41.0
46	36.390111727173945	39.0	35.0	41.0	30.0	41.0
47	36.31980269319714	39.0	35.0	41.0	30.0	41.0
48	36.26288677470032	39.0	35.0	41.0	30.0	41.0
49	36.19224982479002	39.0	35.0	41.0	29.0	41.0
50	36.0804219185011	39.0	35.0	41.0	29.0	41.0
51	34.92730982483288	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	13.0
10	10.0
11	9.0
12	12.0
13	18.0
14	7.0
15	26.0
16	43.0
17	113.0
18	233.0
19	417.0
20	862.0
21	1418.0
22	2066.0
23	2996.0
24	4401.0
25	6939.0
26	9590.0
27	11116.0
28	11373.0
29	11943.0
30	13786.0
31	17126.0
32	21973.0
33	30339.0
34	48711.0
35	62249.0
36	69041.0
37	105467.0
38	186631.0
39	314042.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.818426732221276	18.8132658069411	23.144113694669542	15.224193766168076
2	33.23942363952394	24.92932661498597	24.78605092770204	17.045198817788044
3	30.55222757794433	25.3135026351153	27.995233431136583	16.13903635580379
4	26.840776453492733	24.635595381743443	30.009130208344498	18.51449795641933
5	24.25849205821901	32.49175387873112	25.055670695246075	18.1940833678038
6	24.202553457798505	34.40063182756337	26.551653189250356	14.845161525387764
7	78.66896136378736	4.813398687907617	12.201687588274755	4.315952360030263
8	79.74797624431237	5.290484222528467	10.277485463465236	4.684054069693923
9	73.54629294252041	6.695378957227331	12.316565327069354	7.441762773182907
10	33.57537672825628	33.21070420482529	19.28638634498042	13.927532721938004
11	24.647919019767116	24.53229114648412	32.2098104731634	18.609979360585363
12	22.433843496226824	22.289603350314948	34.4257077518898	20.850845401568424
13	21.58608436226781	24.063885739514728	36.83256783894827	17.5174620592692
14	19.468883349800574	28.195305015399192	32.741762987507045	19.594048647293192
15	18.41322980048566	26.79898324628201	36.03892555022365	18.748861403008682
16	21.64802403859549	26.658493772812125	31.567909675234628	20.12557251335775
17	21.715428980481498	25.81062747678334	31.2312064520139	21.242737090721263
18	22.140326587123834	24.923647025288105	32.03856548566922	20.897460901918844
19	21.199979424882603	27.62241659040299	29.800914306779287	21.376689677935115
20	21.966402547885373	27.343259398649327	31.104433723474706	19.58590432999059
21	21.721644380528225	27.931257675483245	31.715686169448954	18.63141177453958
22	21.003872837201527	24.00676835632674	31.806559604614826	23.182799201856906
23	19.236341658397325	27.494679403235867	31.74654884554302	21.522430092823786
24	20.291245073223845	25.678282320616052	32.289967701352175	21.740504904807935
25	19.21662383755945	29.092465863522676	30.145011712814224	21.54589858610365
26	19.374473566332252	26.94943879224061	30.97766099493552	22.69842664649162
27	21.298568529072	26.175514324353866	30.81916829374409	21.706748852830042
28	18.228696716339858	26.207984431494502	33.228707432546834	22.334611419618803
29	19.61355214399153	24.45888512869093	31.595664651305338	24.331898076012198
30	19.8474869423018	24.99973209482557	33.38720013373827	21.765580829134365
31	20.529573516394723	25.31768195583637	30.667426802948246	23.485317724820664
32	19.662418047807144	25.924326432810453	30.068497994997674	24.34475752438473
33	19.729287179344297	25.24459742425249	31.49032433672037	23.535791059682843
34	18.80394270687102	26.381801308663196	31.584841282258463	23.229414702207325
35	18.749933023706394	25.31189520406873	30.743940520764795	25.19423125146008
36	20.45477439169451	25.898607536065395	30.770516714068023	22.876101358172072
37	19.667347503016614	26.290284901078692	31.583341013281668	22.459026582623025
38	19.45763133247461	25.91135982236815	30.527794626036524	24.103214219120712
39	21.328895394817213	25.60669805800897	29.35115509995006	23.71325144722375
40	21.134503400252473	25.156724527040204	31.079893609497134	22.62887846321019
41	19.63262699241078	26.718933180163013	29.18301781247924	24.465422014946967
42	21.434342871471955	25.19744611355322	30.11864984365054	23.249561171324288
43	22.16358075626416	24.555652477694213	30.374981514542966	22.90578525149866
44	20.472134646997425	24.967904960103564	30.49446722233772	24.065493170561293
45	20.072205802611755	23.995194852791464	30.041385991345592	25.891213353251192
46	22.10121243165739	24.816592117586797	29.317934858321028	23.764260592434784
47	18.769222196265186	25.29196305909131	32.55894449647758	23.379870248165922
48	19.95261293274723	25.033488146803464	30.50250437757055	24.511394542878758
49	20.960900847223325	23.20315999511341	32.16148037969664	23.67445877796662
50	19.658988861574468	23.947722055882874	31.954121774689604	24.43916730785305
51	19.48238577059173	23.526253635473218	29.75483461677772	27.236525977157335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	588.5
2	916.0
3	5813.0
4	10710.0
5	7278.0
6	3846.0
7	3758.0
8	3670.0
9	3666.0
10	3662.0
11	3701.5
12	3741.0
13	3620.0
14	3499.0
15	3338.0
16	3177.0
17	3221.5
18	3266.0
19	3117.0
20	2968.0
21	3247.5
22	3527.0
23	4222.5
24	4918.0
25	5789.0
26	8178.5
27	9697.0
28	11528.0
29	13359.0
30	15317.5
31	17276.0
32	19004.5
33	20733.0
34	24643.5
35	28554.0
36	30944.0
37	33334.0
38	35427.0
39	37520.0
40	42374.5
41	47229.0
42	50719.5
43	54210.0
44	60169.0
45	66128.0
46	85756.0
47	105384.0
48	101907.0
49	98430.0
50	95471.0
51	92512.0
52	80506.0
53	68500.0
54	59521.5
55	50543.0
56	43817.0
57	37091.0
58	33314.5
59	29538.0
60	25663.5
61	21789.0
62	18883.0
63	15977.0
64	13368.0
65	10759.0
66	9075.5
67	7392.0
68	5882.5
69	4373.0
70	3574.0
71	2775.0
72	2366.5
73	1958.0
74	1684.0
75	1183.0
76	956.0
77	711.5
78	467.0
79	379.5
80	292.0
81	184.0
82	76.0
83	57.0
84	38.0
85	29.0
86	20.0
87	15.0
88	10.0
89	7.0
90	4.0
91	5.5
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	933166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.36680787271194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44258996538329	25.2398737901469
2	10.440416064961818	7.176075460314362
3	3.988001169096314	4.111646099234509
4	1.9985416922149537	2.7473399344782363
5	1.247546914735513	2.143710256545496
6	0.9130885714643612	1.8827963703770827
7	0.7406916837558495	1.7818646151986959
8	0.6079775486965528	1.671539808558144
9	0.5340546855913206	1.651837929591465
>10	5.891252168237875	42.133801524490295
>50	0.1591197033956561	3.2995045503015614
>100	0.030442938027842252	1.8101877387727703
>500	0.002510757775480714	0.6268730940665321
>1k	0.0031384472193508924	2.2815290018563927
>5k	6.276894438701785E-4	1.4414198260676419
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	7477	0.801250795678368	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5887	0.6308631047423502	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	4294	0.4601539275970192	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	3874	0.41514585829316547	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	2987	0.32009310240621713	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	2199	0.23564939142660576	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	1640	0.17574579442457183	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1533	0.16427945295906624	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	1452	0.15559932530760873	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT	1109	0.11884273537612815	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT	1036	0.11101990428283928	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT	1029	0.11026976979444172	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTC	995	0.10662625942222499	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2148620931324117E-4	0.0	0.0	0.16674418056380108	0.0
2	3.2148620931324117E-4	0.0	0.0	0.8000720129108861	0.0
3	3.2148620931324117E-4	0.0	0.0	1.1902491089473899	0.0
4	3.2148620931324117E-4	0.0	0.0	1.846295300085944	0.0
5	3.2148620931324117E-4	0.0	0.0	3.671801158636298	0.0
6	3.2148620931324117E-4	0.0	0.0	4.823793408675413	0.0
7	3.2148620931324117E-4	0.0	0.0	5.795539057359569	0.0
8	3.2148620931324117E-4	0.0	0.0	7.303095054899129	0.0
9	3.2148620931324117E-4	0.0	0.0	7.894201031756408	0.0
10	3.2148620931324117E-4	0.0	0.0	9.398863653412148	0.0
11	3.2148620931324117E-4	0.0	0.0	10.673985121618234	0.0
12	3.2148620931324117E-4	0.0	0.0	12.205866908995826	0.0
13	3.2148620931324117E-4	0.0	0.0	12.70106283340799	0.0
14	3.2148620931324117E-4	0.0	0.0	12.934676145508945	0.0
15	3.2148620931324117E-4	0.0	0.0	13.305992717265738	0.0
16	3.2148620931324117E-4	0.0	0.0	13.953358780752835	0.0
17	3.2148620931324117E-4	0.0	0.0	14.785150766315962	0.0
18	3.2148620931324117E-4	0.0	0.0	15.673202838508905	0.0
19	3.2148620931324117E-4	0.0	0.0	16.29292108799506	0.0
20	3.2148620931324117E-4	0.0	0.0	16.81522901605931	0.0
21	3.2148620931324117E-4	0.0	0.0	17.421766330963624	0.0
22	3.2148620931324117E-4	0.0	0.0	18.105353174033343	0.0
23	3.2148620931324117E-4	0.0	0.0	18.74875424093891	0.0
24	3.2148620931324117E-4	0.0	0.0	19.270740682793843	0.0
25	3.2148620931324117E-4	0.0	0.0	19.73164474487926	0.0
26	3.2148620931324117E-4	0.0	0.0	20.185154624150474	0.0
27	3.2148620931324117E-4	0.0	0.0	20.656346244933914	0.0
28	3.2148620931324117E-4	0.0	0.0	21.148327307252945	0.0
29	4.286482790843215E-4	0.0	0.0	21.64309458338602	0.0
30	4.286482790843215E-4	0.0	0.0	22.24095177063888	0.0
31	4.286482790843215E-4	0.0	0.0	22.76175942972633	0.0
32	4.286482790843215E-4	0.0	0.0	23.249239685114976	0.0
33	4.286482790843215E-4	0.0	0.0	23.755366140643787	0.0
34	4.286482790843215E-4	0.0	0.0	24.230094109729674	0.0
35	4.286482790843215E-4	0.0	0.0	24.736434889398026	0.0
36	4.286482790843215E-4	0.0	0.0	25.19958935494864	0.0
37	4.286482790843215E-4	0.0	0.0	25.680425562011475	0.0
38	4.286482790843215E-4	0.0	0.0	26.167798655330348	0.0
39	4.286482790843215E-4	0.0	0.0	26.72557722848882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	45	3.8380676E-10	45.0	1
GATCGAC	20	7.032605E-4	45.0	9
ATAGCGA	25	3.89024E-5	45.0	10
CTACGCG	25	3.89024E-5	45.0	1
CGTCGAA	25	3.89024E-5	45.0	42
CGATCCC	20	7.032605E-4	45.0	34
TATCGCG	35	1.2115925E-7	45.0	1
CGGACTA	20	7.032605E-4	45.0	27
CGTTTTT	3725	0.0	42.52349	1
ACGACGC	70	0.0	41.785713	14
TTAGGCG	50	1.0804797E-9	40.5	1
ATTAGCG	50	1.0804797E-9	40.5	1
GCGAATG	50	1.0804797E-9	40.5	1
GTTAGCG	95	0.0	40.263157	1
TTGCGCG	45	1.927583E-8	40.0	1
ATAACGG	80	0.0	39.375	2
GCGAGAC	260	0.0	38.94231	21
CGAGACA	260	0.0	38.94231	22
CTAAGCG	35	6.2470262E-6	38.571426	1
GCGTTAG	70	0.0	38.571426	1
>>END_MODULE