##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935904.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526126
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73578192296142	31.0	31.0	34.0	30.0	34.0
2	31.77911374841768	31.0	31.0	34.0	30.0	34.0
3	31.84894492954159	33.0	31.0	34.0	30.0	34.0
4	35.585893873330726	37.0	35.0	37.0	33.0	37.0
5	33.43824863245686	37.0	35.0	37.0	30.0	37.0
6	34.403914651623374	37.0	35.0	37.0	30.0	37.0
7	35.4801340363335	37.0	35.0	37.0	32.0	37.0
8	35.365914248678074	37.0	35.0	37.0	33.0	37.0
9	37.258038948844955	39.0	37.0	39.0	34.0	39.0
10	36.85238136872156	39.0	37.0	39.0	32.0	39.0
11	36.882691598590455	39.0	37.0	39.0	33.0	39.0
12	36.81321394494855	39.0	35.0	39.0	33.0	39.0
13	36.62426300924113	39.0	35.0	39.0	32.0	39.0
14	37.7054317786994	40.0	36.0	41.0	33.0	41.0
15	37.6497397961705	40.0	36.0	41.0	32.0	41.0
16	37.87041507167485	40.0	36.0	41.0	33.0	41.0
17	37.68287634521008	39.0	36.0	41.0	32.0	41.0
18	37.70691811467215	39.0	36.0	41.0	33.0	41.0
19	37.62438845447669	39.0	36.0	41.0	33.0	41.0
20	37.4346050185697	39.0	35.0	41.0	32.0	41.0
21	37.575174007747194	39.0	36.0	41.0	33.0	41.0
22	37.64487024020862	39.0	36.0	41.0	33.0	41.0
23	37.57334174703398	39.0	36.0	41.0	33.0	41.0
24	37.523735759114736	39.0	35.0	41.0	32.0	41.0
25	37.46215355257106	39.0	35.0	41.0	33.0	41.0
26	37.40412752838674	39.0	35.0	41.0	32.0	41.0
27	37.35258094068721	39.0	35.0	41.0	32.0	41.0
28	37.240254235677384	39.0	35.0	41.0	32.0	41.0
29	37.24146307158361	39.0	35.0	41.0	32.0	41.0
30	37.1310579595002	39.0	35.0	41.0	32.0	41.0
31	37.233980073214404	39.0	35.0	41.0	32.0	41.0
32	37.002564024587265	39.0	35.0	41.0	31.0	41.0
33	36.803739028293606	39.0	35.0	41.0	31.0	41.0
34	36.75285768047958	39.0	35.0	41.0	31.0	41.0
35	36.60691735439799	39.0	35.0	41.0	30.0	41.0
36	36.62549465337201	39.0	35.0	41.0	31.0	41.0
37	36.561625922307584	39.0	35.0	41.0	30.0	41.0
38	36.46379954611633	39.0	35.0	41.0	30.0	41.0
39	36.40516720329351	39.0	35.0	41.0	30.0	41.0
40	36.16299137468971	39.0	35.0	41.0	30.0	41.0
41	36.10146048665148	39.0	35.0	41.0	29.0	41.0
42	36.219415121092666	39.0	35.0	41.0	30.0	41.0
43	36.256176277165544	39.0	35.0	41.0	30.0	41.0
44	36.23061966144992	39.0	35.0	41.0	30.0	41.0
45	36.245505829402084	39.0	35.0	40.0	30.0	41.0
46	36.12238133070785	39.0	35.0	40.0	30.0	41.0
47	35.86455145725549	38.0	35.0	40.0	29.0	41.0
48	35.88299950962317	38.0	35.0	40.0	28.0	41.0
49	35.859856764349225	39.0	35.0	40.0	28.0	41.0
50	35.75296031749048	38.0	35.0	40.0	28.0	41.0
51	34.44867959386155	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	8.0
10	8.0
11	10.0
12	5.0
13	6.0
14	6.0
15	18.0
16	29.0
17	50.0
18	140.0
19	376.0
20	971.0
21	1739.0
22	2631.0
23	3140.0
24	3744.0
25	4142.0
26	4791.0
27	5306.0
28	5918.0
29	7042.0
30	8679.0
31	11667.0
32	16155.0
33	22380.0
34	35540.0
35	45954.0
36	40492.0
37	59769.0
38	101169.0
39	144168.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.355899537373176	17.1937900807031	19.576679350573816	10.873631031349905
2	26.81505951045947	36.31981692598351	21.307633532651874	15.557490030905145
3	26.21900457304904	36.04839905269841	23.688432048596724	14.044164325655832
4	24.405940782246077	22.29218856319589	37.38401067424913	15.917859980308899
5	31.331087990329316	29.392388895435694	21.290147227090088	17.986375887144902
6	21.381190057134603	41.29029928192106	23.738420074278785	13.590090586665552
7	69.51395673279784	4.963829957082524	21.11414376023994	4.408069549879687
8	67.8740453807643	15.497618441209902	10.449207984399173	6.179128193626622
9	63.68398444479079	5.9763250628176525	11.315540383862421	19.024150108529135
10	41.02477353333612	21.56859763630765	20.897275557566058	16.509353272790168
11	32.997799006321685	22.322789597929013	25.293370789506696	19.38604060624261
12	25.168495759570902	19.849997909246074	29.605075590257847	25.376430740925176
13	23.87032764014704	24.01687048349635	36.44545223007417	15.66734964628245
14	20.4131709894588	32.03966350265906	26.468564564381914	21.078600943500227
15	16.247818963518245	24.42532777319501	40.072530154373666	19.254323108913074
16	17.8386926325633	25.86053530903244	27.863857707089178	28.436914351315085
17	18.705785306181408	26.70139852430787	31.182454393054137	23.41036177645659
18	18.987276812018415	24.729247366600397	31.052827649650467	25.230648171730724
19	18.64534351087002	27.694126502016626	27.98816253140883	25.672367455704524
20	21.288056473164225	26.37257995233081	34.03196192547032	18.30740164903464
21	20.35957166154115	32.99399763554738	28.135085511835566	18.5113451910759
22	18.094524885673774	23.286627157753088	31.713886027301445	26.9049619292717
23	18.680126053454877	30.616810421838114	29.21011316680795	21.49295035789906
24	21.567457225075362	25.014730311750416	27.874501545257218	25.543310917917
25	17.24776954569818	34.510744574493565	26.18251141361575	22.05897446619251
26	17.02957086325329	25.31446839730407	30.10514591561717	27.55081482382547
27	21.824429889418123	29.064900803229644	25.888665452762265	23.222003854589964
28	15.76694556056914	26.20874087195843	33.120393213792894	24.90392035367954
29	21.101409168146034	21.87688880610348	28.489943473616584	28.5317585521339
30	18.553920543748077	31.915548746878127	29.339169704595474	20.191361004778326
31	23.614115249959138	24.28809828824274	22.723834214617792	29.373952247180334
32	26.248085059472448	30.302246990264692	22.58451397573965	20.865153974523214
33	20.793498135427633	25.45568932156936	26.30206452446752	27.448748018535486
34	25.438583153085006	23.56108612765763	25.20099747969118	25.799333239566185
35	19.93685923143886	30.50619053230595	24.87845116949172	24.678499066763475
36	19.228093650570397	29.426791300943123	23.930009161303566	27.415105887182918
37	22.06163542573452	29.367870053941452	28.68381338310595	19.886681137218083
38	18.715668870194595	26.854593766512203	25.934471970592593	28.49526539270061
39	28.42132873114045	24.67222680498588	25.84761064839981	21.05883381547386
40	20.326119598727303	26.122829892459222	31.13949890330453	22.411551605508947
41	21.893044631894263	29.702010545002526	23.526493653611492	24.87845116949172
42	19.420443011750038	28.4371044198538	29.854825650129435	22.287626918266724
43	21.999292945035982	27.449698361229057	24.72639633851967	25.824612355215294
44	21.264107837286126	24.205228405362973	29.242234749850798	25.288429007500106
45	19.878318121514617	23.039538057423506	26.804225603752712	30.277918217309164
46	27.935893683262186	26.61301665380536	24.48120792357724	20.969881739355213
47	17.377396289101853	24.45725928769914	36.0470685729274	22.11827585027161
48	22.154959078243614	26.506388203586212	25.679019854559552	25.659632863610614
49	20.11723427467945	21.34545717185617	34.356028783979504	24.181279769484874
50	22.54764067922893	21.99625184841654	29.718736576409455	25.737370895945077
51	20.308063087549368	22.371637212378783	26.01506103100778	31.305238669064067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	276.5
2	400.0
3	1310.0
4	2220.0
5	1559.5
6	899.0
7	927.5
8	956.0
9	914.5
10	873.0
11	903.5
12	934.0
13	963.0
14	992.0
15	996.0
16	1000.0
17	1003.0
18	1006.0
19	1099.5
20	1193.0
21	1334.5
22	1476.0
23	1544.0
24	1612.0
25	1908.5
26	2625.0
27	3045.0
28	3950.0
29	4855.0
30	5622.5
31	6390.0
32	7265.0
33	8140.0
34	9640.0
35	11140.0
36	12992.0
37	14844.0
38	15776.0
39	16708.0
40	19327.5
41	21947.0
42	25975.0
43	30003.0
44	34507.5
45	39012.0
46	72869.5
47	106727.0
48	82158.5
49	57590.0
50	57140.0
51	56690.0
52	47172.5
53	37655.0
54	33000.0
55	28345.0
56	24398.5
57	20452.0
58	17375.0
59	14298.0
60	12741.5
61	11185.0
62	9437.0
63	7689.0
64	6739.0
65	5789.0
66	4445.5
67	3102.0
68	2505.5
69	1909.0
70	1497.0
71	1085.0
72	808.5
73	532.0
74	531.5
75	408.0
76	285.0
77	204.0
78	123.0
79	104.5
80	86.0
81	58.5
82	31.0
83	21.0
84	11.0
85	7.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.181011031148596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43368833396111	22.897366459991694
2	11.222172795418846	6.998373874548215
3	4.209671976259513	3.9378548498779504
4	2.0173277828108622	2.5160927939707216
5	1.1875325034930386	1.8514232045631975
6	0.7961473811649827	1.4894808164715234
7	0.6055698056506907	1.3217595153087291
8	0.49012612953172163	1.2226102601266224
9	0.3785028243830033	1.0621890668166596
>10	5.491691762854203	38.02952281039819
>50	0.12170135835741727	2.3512327301778257
>100	0.03302448920251524	1.7682871943989442
>500	0.004892516918891148	1.0383181034726896
>1k	0.006115646148613933	4.661085289840762
>5k	0.0	0.0
>10k+	0.0018346938445841802	8.854403030036252
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC	17438	3.314415178113227	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG	14841	2.8208071830702153	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC	14154	2.6902300969729684	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	4930	0.937037895865249	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCT	3173	0.6030874733428875	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGTGAAT	2897	0.5506285566575307	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT	2586	0.49151724111714684	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTA	2462	0.46794874231647937	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTT	2442	0.4641473715421781	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTC	2427	0.46129634346145215	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1379	0.26210451488806863	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1120	0.21287676336086792	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	1027	0.19520038926036729	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC	868	0.16497949160467265	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATG	771	0.14654284334931178	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG	758	0.14407195234601597	No Hit
GAATATGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC	678	0.12886646924881112	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACGTGAATCGT	643	0.12221407039378399	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTAT	632	0.12012331646791834	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	565	0.10738872437400927	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCACGTGAA	530	0.10073632551898214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21078600943500225	0.0
2	0.0	0.0	0.0	1.6908497204091795	0.0
3	0.0	0.0	0.0	2.0669953585262846	0.0
4	0.0	0.0	0.0	3.023610313879185	0.0
5	0.0	0.0	0.0	6.979696878694457	0.0
6	0.0	0.0	0.0	8.213241694955201	0.0
7	0.0	0.0	0.0	9.57508277484861	0.0
8	0.0	0.0	0.0	11.18667391461361	0.0
9	0.0	0.0	0.0	11.559018181956413	0.0
10	0.0	0.0	0.0	15.519856460239563	0.0
11	0.0	0.0	0.0	16.923132481572853	0.0
12	0.0	0.0	0.0	21.663061700049038	0.0
13	0.0	0.0	0.0	22.30397281259622	0.0
14	0.0	0.0	0.0	22.630320493569982	0.0
15	0.0	0.0	0.0	23.48847994586848	0.0
16	0.0	0.0	0.0	24.362985292496475	0.0
17	0.0	0.0	0.0	25.337276621949876	0.0
18	0.0	0.0	0.0	26.29180082337691	0.0
19	0.0	0.0	0.0	27.530667558721674	0.0
20	0.0	0.0	0.0	28.18925504536936	0.0
21	0.0	0.0	0.0	28.889087404918214	0.0
22	1.9006853871506065E-4	0.0	0.0	29.68851567875376	0.0
23	1.9006853871506065E-4	0.0	0.0	30.44650901114942	0.0
24	1.9006853871506065E-4	0.0	0.0	31.02070606660762	0.0
25	1.9006853871506065E-4	0.0	0.0	31.552137700854928	0.0
26	1.9006853871506065E-4	0.0	0.0	32.07748714186336	0.0
27	1.9006853871506065E-4	0.0	0.0	32.630016383908035	0.0
28	1.9006853871506065E-4	0.0	0.0	33.14263123282256	0.0
29	1.9006853871506065E-4	0.0	0.0	33.616662168377914	0.0
30	1.9006853871506065E-4	0.0	0.0	34.19504073168785	0.0
31	5.702056161451819E-4	0.0	0.0	34.69758194805047	0.0
32	5.702056161451819E-4	0.0	0.0	35.197652273409794	0.0
33	5.702056161451819E-4	0.0	0.0	35.684988006675205	0.0
34	5.702056161451819E-4	0.0	0.0	36.18372785226352	0.0
35	5.702056161451819E-4	0.0	0.0	36.69938379779749	0.0
36	7.602741548602426E-4	0.0	0.0	37.17645582997229	0.0
37	9.503426935753033E-4	0.0	0.0	37.66721279693458	0.0
38	9.503426935753033E-4	0.0	0.0	38.16044065490016	0.0
39	9.503426935753033E-4	0.0	0.0	38.686170232986015	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGA	25	3.887911E-5	45.000004	9
TAAAGCG	20	7.0297945E-4	45.000004	1
CTGAGCG	20	7.0297945E-4	45.000004	1
ATTACGG	25	3.887911E-5	45.000004	2
TTCACGG	25	3.887911E-5	45.000004	2
TTTCACG	20	7.0297945E-4	45.000004	1
TATTGCG	20	7.0297945E-4	45.000004	1
ATCCGGC	40	6.8030204E-9	45.000004	6
ACGGCCG	20	7.0297945E-4	45.000004	30
TACGATG	20	7.0297945E-4	45.000004	1
CGTAAGG	20	7.0297945E-4	45.000004	2
GTAAGCG	25	3.887911E-5	45.000004	1
CGATCTA	20	7.0297945E-4	45.000004	43
AATCGTG	20	7.0297945E-4	45.000004	27
TGCGTTC	20	7.0297945E-4	45.000004	14
TCACGTT	20	7.0297945E-4	45.000004	44
GCGAGCC	20	7.0297945E-4	45.000004	33
CTACGAA	35	1.2102464E-7	45.0	11
TAATCCG	35	1.2102464E-7	45.0	4
CGCGCGG	30	2.1632495E-6	44.999996	2
>>END_MODULE