##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935903.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353924
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78317096325765	31.0	31.0	34.0	30.0	34.0
2	31.8149800522146	33.0	31.0	34.0	30.0	34.0
3	31.867465896633174	33.0	31.0	34.0	30.0	34.0
4	35.60076457092483	37.0	35.0	37.0	33.0	37.0
5	33.498146494727685	37.0	35.0	37.0	32.0	37.0
6	34.48336083452945	37.0	35.0	37.0	30.0	37.0
7	35.582950011866956	37.0	35.0	37.0	32.0	37.0
8	35.56217436511793	37.0	35.0	37.0	33.0	37.0
9	37.45386580169754	39.0	37.0	39.0	35.0	39.0
10	37.02933398130672	39.0	37.0	39.0	33.0	39.0
11	37.02762740023282	39.0	37.0	39.0	33.0	39.0
12	36.919564652298234	39.0	35.0	39.0	33.0	39.0
13	36.74277528508946	39.0	35.0	39.0	33.0	39.0
14	37.76861699121845	40.0	36.0	41.0	33.0	41.0
15	37.84855223155254	40.0	36.0	41.0	33.0	41.0
16	37.92414190617195	40.0	36.0	41.0	33.0	41.0
17	37.902391473875745	40.0	36.0	41.0	33.0	41.0
18	37.89937952780823	39.0	36.0	41.0	33.0	41.0
19	37.82164249951967	39.0	36.0	41.0	33.0	41.0
20	37.81828019574824	40.0	36.0	41.0	33.0	41.0
21	37.76785694103819	39.0	36.0	41.0	33.0	41.0
22	37.850727839875226	39.0	36.0	41.0	33.0	41.0
23	37.83114736497101	39.0	36.0	41.0	34.0	41.0
24	37.77297950972525	39.0	36.0	41.0	33.0	41.0
25	37.612495903075235	39.0	35.0	41.0	33.0	41.0
26	37.64330195183147	39.0	35.0	41.0	33.0	41.0
27	37.63304551259593	39.0	35.0	41.0	33.0	41.0
28	37.55876120297013	39.0	36.0	41.0	33.0	41.0
29	37.546391315649686	39.0	36.0	41.0	33.0	41.0
30	37.38162430352279	39.0	36.0	41.0	33.0	41.0
31	37.37758106260101	39.0	35.0	41.0	33.0	41.0
32	37.264172534216385	39.0	35.0	41.0	33.0	41.0
33	37.149800522145995	39.0	35.0	41.0	32.0	41.0
34	37.07992393847267	39.0	35.0	41.0	32.0	41.0
35	37.05266102327053	39.0	35.0	41.0	32.0	41.0
36	36.95345045829048	39.0	35.0	41.0	32.0	41.0
37	36.94714401962003	39.0	35.0	41.0	32.0	41.0
38	36.825035883409996	39.0	35.0	41.0	31.0	41.0
39	36.81566946576101	39.0	35.0	41.0	31.0	41.0
40	36.68879759496389	39.0	35.0	41.0	31.0	41.0
41	36.61976299996609	39.0	35.0	41.0	31.0	41.0
42	36.69196776709124	39.0	35.0	41.0	31.0	41.0
43	36.636667759179936	39.0	35.0	41.0	31.0	41.0
44	36.516068421469015	39.0	35.0	41.0	31.0	41.0
45	36.47981770097535	39.0	35.0	40.0	31.0	41.0
46	36.35349397045693	39.0	35.0	40.0	31.0	41.0
47	36.21460257004328	38.0	35.0	40.0	30.0	41.0
48	36.188017766526144	38.0	35.0	40.0	30.0	41.0
49	36.16768854330308	38.0	35.0	40.0	30.0	41.0
50	36.02050440207502	38.0	35.0	40.0	30.0	41.0
51	34.74901956352211	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	4.0
9	6.0
10	2.0
11	7.0
12	2.0
13	7.0
14	5.0
15	19.0
16	18.0
17	31.0
18	74.0
19	156.0
20	343.0
21	543.0
22	821.0
23	1141.0
24	1607.0
25	2131.0
26	2724.0
27	3214.0
28	3779.0
29	4415.0
30	5721.0
31	7359.0
32	10060.0
33	14274.0
34	24522.0
35	33317.0
36	27900.0
37	41760.0
38	69999.0
39	97913.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72316090460099	19.109752376216363	22.91198110328771	13.255105615894939
2	29.30657429278602	29.415637255455973	24.163662255173428	17.11412619658458
3	29.800183090154945	27.593777195104035	26.363852126445224	16.242187588295792
4	27.715554751867632	23.559577762457476	30.228523637843153	18.496343847831735
5	25.383133102021905	31.21997943061222	23.861337462280037	19.535550005085838
6	22.347735672065188	36.25552378476735	25.758354901052204	15.638385642115257
7	74.48293984019168	5.316113063821612	14.908567941139905	5.292379154846803
8	74.1690306393463	8.101739356472011	10.654264757405544	7.074965246776143
9	69.43948418304495	5.942236186299884	12.64791311128943	11.97036651936574
10	39.68959437619376	22.449452424814368	20.415117369830813	17.445835829161062
11	31.184096020614593	23.13293249398176	25.6148212610617	20.06815022434195
12	27.0863236174998	19.94637266757835	31.123348515500503	21.843955199421345
13	23.544037703009685	24.89545778189668	33.59619579344718	17.964308721646454
14	19.774584374046405	30.045150936359217	28.453001209299174	21.727263480295207
15	16.671093229054826	24.28770018422034	39.41580678337722	19.625399803347612
16	18.218318056983986	24.586916965224173	32.664639866185965	24.530125111605884
17	18.103604163605745	23.536974039624326	31.01739356472011	27.34202823204982
18	19.58217018342921	24.30437042980979	32.46968275675003	23.64377663001096
19	20.354652411252133	25.940032323323653	28.75137035069676	24.953944914727455
20	22.43023926040619	25.54305444106644	30.763384229382577	21.263322069144788
21	20.867191826493823	28.68355918219731	29.989771815418	20.459477175890868
22	18.255614199658684	24.68298278726506	29.058498434692194	28.002904578384058
23	17.98465207219629	28.291384591042146	29.15936754783513	24.564595788926436
24	21.643629705812547	24.208022061233486	29.197511330116072	24.950836902837896
25	17.47663340152123	29.68010081260384	28.02861631310677	24.814649472768167
26	17.593042574111955	27.676280783445034	29.940326171720482	24.790350470722526
27	22.323719216554966	27.272804330873296	27.63785445462868	22.76562199794306
28	17.3523129259389	26.27795797967925	31.375945118160963	24.993783976220886
29	20.586905663362757	25.44020750217561	28.948305285880583	25.024581548581047
30	22.785117708886656	27.87180298595179	28.069303014206437	21.27377629095512
31	22.698093375979024	26.877521727828572	24.727060046789706	25.697324849402698
32	25.27067958092698	29.991467094630487	23.244820922005854	21.493032402436683
33	22.503136266543102	26.960307862704987	23.85822945039048	26.67832642036143
34	19.90512087340785	26.719578214531936	28.938416157141084	24.436884754919134
35	20.853629592793933	27.763587662888078	25.972242628360892	25.410540115957097
36	21.82248166272985	27.410121947084686	26.256201896452346	24.511194493733118
37	23.588680055605156	24.44564369751698	29.07121302878584	22.89446321809202
38	19.870085103016464	28.895186537222678	23.756230151105886	27.478498208654965
39	21.94821487098925	25.49389134390434	28.825962636046153	23.73193114906025
40	21.06412676167765	26.922729173494876	29.87675320125225	22.13639086357523
41	18.107842361636962	26.134424339688746	27.49347317503193	28.264260123642366
42	20.9669307534951	25.889739040019894	27.837049762095816	25.30628044438919
43	23.186616335710493	23.427345983883548	26.470372170296447	26.915665510109516
44	21.4187226636227	24.713780359625233	27.225619059459095	26.641877917292977
45	20.775929295554977	23.701698669770913	26.645551022253365	28.876821012420745
46	24.716605824979375	25.354030808874224	27.3259230795312	22.603440286615207
47	18.65004916309716	24.161119336354698	33.09128513466168	24.097546365886462
48	20.508640273052972	24.610933420734398	27.764717849029736	27.115708457182897
49	20.91918038901007	20.56260666131712	32.88502616380918	25.633186785863632
50	21.302030944496558	21.370407206066837	30.847300550400654	26.480261299035952
51	19.875170940653923	22.111526768458763	26.80632000090415	31.20698228998316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	334.5
2	457.0
3	1071.0
4	1685.0
5	1177.0
6	669.0
7	682.0
8	695.0
9	749.0
10	803.0
11	821.5
12	840.0
13	871.0
14	902.0
15	876.5
16	851.0
17	826.0
18	801.0
19	742.0
20	683.0
21	807.5
22	932.0
23	974.0
24	1016.0
25	1216.5
26	1571.0
27	1725.0
28	2143.0
29	2561.0
30	3138.0
31	3715.0
32	4174.5
33	4634.0
34	5484.0
35	6334.0
36	7181.5
37	8029.0
38	9419.0
39	10809.0
40	12132.5
41	13456.0
42	16280.5
43	19105.0
44	23094.0
45	27083.0
46	40326.5
47	53570.0
48	49111.0
49	44652.0
50	43907.5
51	43163.0
52	35778.5
53	28394.0
54	24305.5
55	20217.0
56	17499.0
57	14781.0
58	12970.5
59	11160.0
60	9984.5
61	8809.0
62	8042.5
63	7276.0
64	6076.5
65	4877.0
66	3651.5
67	2426.0
68	2074.0
69	1722.0
70	1623.5
71	1525.0
72	1175.0
73	825.0
74	694.5
75	399.5
76	235.0
77	213.0
78	191.0
79	127.0
80	63.0
81	51.5
82	40.0
83	23.5
84	7.0
85	7.0
86	7.0
87	4.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.725569262077094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.49191468794918	19.765100154247545
2	14.298160952825741	8.500419474470956
3	5.652774025366242	5.040957774416838
4	2.765029479729255	3.287683012455079
5	1.6002640983589154	2.3784380646691647
6	1.034835980912648	1.8456653175305044
7	0.7422750563311625	1.5445184018938907
8	0.5631710011226576	1.3392462880211886
9	0.48158041953024167	1.2883726904405712
>10	6.109304122669111	41.48494173100223
>50	0.1960151317380359	3.6083892778532314
>100	0.051740034773491705	2.666658830256126
>500	0.0069650046810469605	1.6580844501348537
>1k	0.004975003343604972	4.111789159075052
>5k	9.950006687209944E-4	1.4797353735327636
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	5003	1.4135803166781569	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	4254	1.2019529616527842	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	4114	1.1623964466947707	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2845	0.8038448932539188	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	1632	0.4611159457962727	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	1057	0.29865168793300256	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	967	0.2732224997457081	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCT	961	0.2715272205332218	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGATACT	793	0.22405940258360554	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCT	766	0.2164306461274172	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTA	720	0.2034335054983556	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTC	705	0.19919530746713981	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	694	0.19608729557758164	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTT	493	0.1392954419592907	No Hit
ACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	413	0.11669171912614007	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	373	0.10538985770956477	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8254653541438275E-4	0.0	0.0	0.3147568404516224	0.0
2	2.8254653541438275E-4	0.0	0.0	1.9716097241215629	0.0
3	2.8254653541438275E-4	0.0	0.0	2.553655587075191	0.0
4	2.8254653541438275E-4	0.0	0.0	3.4795605836281234	0.0
5	2.8254653541438275E-4	0.0	0.0	6.5177834789389815	0.0
6	2.8254653541438275E-4	0.0	0.0	7.903674235146529	0.0
7	2.8254653541438275E-4	0.0	0.0	9.386195906465796	0.0
8	2.8254653541438275E-4	0.0	0.0	11.525920819158916	0.0
9	2.8254653541438275E-4	0.0	0.0	12.226071133915756	0.0
10	2.8254653541438275E-4	0.0	0.0	14.978356935387259	0.0
11	2.8254653541438275E-4	0.0	0.0	17.09943377674303	0.0
12	2.8254653541438275E-4	0.0	0.0	20.297295464563014	0.0
13	2.8254653541438275E-4	0.0	0.0	21.105378555848148	0.0
14	2.8254653541438275E-4	0.0	0.0	21.43680564188922	0.0
15	2.8254653541438275E-4	0.0	0.0	22.13695595664606	0.0
16	2.8254653541438275E-4	0.0	0.0	23.24058272397464	0.0
17	2.8254653541438275E-4	0.0	0.0	24.52419163436218	0.0
18	2.8254653541438275E-4	0.0	0.0	25.988065234344095	0.0
19	2.8254653541438275E-4	0.0	0.0	27.02868412427527	0.0
20	5.650930708287655E-4	0.0	0.0	27.807664922412723	0.0
21	5.650930708287655E-4	0.0	0.0	28.699099241645097	0.0
22	5.650930708287655E-4	0.0	0.0	29.644499949141622	0.0
23	5.650930708287655E-4	0.0	0.0	30.615329844825443	0.0
24	5.650930708287655E-4	0.0	0.0	31.32875984674676	0.0
25	5.650930708287655E-4	0.0	0.0	31.963359365287463	0.0
26	5.650930708287655E-4	0.0	0.0	32.564335846113856	0.0
27	8.476396062431482E-4	0.0	0.0	33.16474723386942	0.0
28	8.476396062431482E-4	0.0	0.0	33.756964772097966	0.0
29	8.476396062431482E-4	0.0	0.0	34.36867802127011	0.0
30	8.476396062431482E-4	0.0	0.0	35.09114951232468	0.0
31	0.001130186141657531	0.0	0.0	35.71981555362168	0.0
32	0.001130186141657531	0.0	0.0	36.3272906047626	0.0
33	0.001130186141657531	0.0	0.0	36.94663261039093	0.0
34	0.001130186141657531	0.0	0.0	37.51116058814887	0.0
35	0.0014127326770719138	0.0	0.0	38.11694036007731	0.0
36	0.0014127326770719138	0.0	0.0	38.6845763497248	0.0
37	0.0014127326770719138	0.0	0.0	39.26464438693053	0.0
38	0.0014127326770719138	0.0	0.0	39.785377651699235	0.0
39	0.0014127326770719138	0.0	0.0	40.337191035363524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCGC	30	2.1612286E-6	45.000004	38
ATCGTGA	30	2.1612286E-6	45.000004	30
GTTGTCG	30	2.1612286E-6	45.000004	37
CTACGAA	30	2.1612286E-6	45.000004	11
TTGACGG	65	0.0	45.000004	2
CGAATGG	30	2.1612286E-6	45.000004	2
TTCGGGC	30	2.1612286E-6	45.000004	4
GAACCGC	30	2.1612286E-6	45.000004	9
GCTACGA	30	2.1612286E-6	45.000004	10
CTAGGCG	30	2.1612286E-6	45.000004	1
CTTCGTA	20	7.0266635E-4	45.0	19
CTCACGA	20	7.0266635E-4	45.0	24
GCGCGCC	20	7.0266635E-4	45.0	16
GACGTTG	20	7.0266635E-4	45.0	1
TGGCGTG	20	7.0266635E-4	45.0	1
TAGCGGT	20	7.0266635E-4	45.0	40
CGCGCCT	20	7.0266635E-4	45.0	17
CCTCGAT	40	6.7921064E-9	45.0	15
TCAGGTC	20	7.0266635E-4	45.0	19
CTGCGGT	20	7.0266635E-4	45.0	15
>>END_MODULE