SRR2935902.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935902.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319111
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	9039	2.8325566965726656	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	7990	2.5038309553728952	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	7539	2.3625008225977795	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2749	0.8614557317046418	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTT	2068	0.6480503649200435	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	1891	0.5925837717910069	Illumina Paired End PCR Primer 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT	1709	0.5355503257487207	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCGATTT	1575	0.4935586676736308	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTC	1300	0.4073817574449017	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTA	1128	0.35348201722911465	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	996	0.3121171003193246	Illumina Paired End PCR Primer 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	834	0.2613510659300369	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	693	0.21716581377639754	TruSeq Adapter, Index 1 (95% over 24bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	419	0.1313022741303183	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATG	400	0.12534823305996973	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	395	0.12378138014672012	No Hit
GAATATGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	384	0.12033430373757095	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	351	0.10999307451012344	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTAT	337	0.10560588635302451	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGT	320	0.1002785864479758	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1605556E-6	45.000004	6
CGGCGAA	35	1.2082455E-7	45.000004	31
CGAACAC	30	2.1605556E-6	45.000004	34
GGTAATT	30	2.1605556E-6	45.000004	8
ACGGCTA	35	1.2082455E-7	45.000004	30
CTACGAA	35	1.2082455E-7	45.000004	11
CGAATAT	35	1.2082455E-7	45.000004	14
TCATCGA	30	2.1605556E-6	45.000004	16
CGTGGAC	20	7.0256204E-4	45.0	38
AGCCGAC	25	3.884452E-5	45.0	24
CCCTATG	25	3.884452E-5	45.0	30
GCGCGGA	20	7.0256204E-4	45.0	30
ATAGCGC	20	7.0256204E-4	45.0	14
AGGACGA	20	7.0256204E-4	45.0	38
CGCGCCT	20	7.0256204E-4	45.0	17
AATGGGC	50	2.1827873E-11	45.0	4
CGATTGA	20	7.0256204E-4	45.0	10
ACGGGTC	40	6.7884685E-9	45.0	5
TAGTTCG	25	3.884452E-5	45.0	30
AATGCGG	20	7.0256204E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content