SRR2935901.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607341
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	19204	3.1619798432840858	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	14096	2.320936673137496	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	12206	2.009744114097352	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	5518	0.9085505506791078	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	5472	0.9009765518876546	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT	3173	0.5224412644626331	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	2899	0.4773265760091942	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTC	2775	0.4569097097017985	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2707	0.4457133636622589	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCGCTGT	2616	0.43073001822699275	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTA	2251	0.3706319843382877	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	2055	0.3383601634007913	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	1572	0.2588331760905323	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	1024	0.16860379918365465	No Hit
GAATATGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	815	0.13419150032683452	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATG	727	0.1197021113344892	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGT	667	0.10982298247607192	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	628	0.1034015487181007	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	70	0.0	45.000004	31
ACGCCCC	35	1.2106648E-7	45.000004	36
GCGCGAG	35	1.2106648E-7	45.000004	1
ATTGACG	35	1.2106648E-7	45.000004	1
ACGACGG	25	3.8886243E-5	45.000004	2
CTATGCG	20	7.030656E-4	45.0	1
TTAGGCG	20	7.030656E-4	45.0	1
GCGACAA	20	7.030656E-4	45.0	11
AAGCGAA	20	7.030656E-4	45.0	22
GCGCATT	20	7.030656E-4	45.0	11
TCGGACA	20	7.030656E-4	45.0	11
CTCGCGC	20	7.030656E-4	45.0	33
CTCGATG	20	7.030656E-4	45.0	1
TAAGCGA	20	7.030656E-4	45.0	21
AGGGCGC	40	6.8066583E-9	45.0	6
TCGCGCA	20	7.030656E-4	45.0	34
GTCGGAC	20	7.030656E-4	45.0	10
AGTCGAG	20	7.030656E-4	45.0	1
GTCGAAT	65	0.0	44.999996	43
CGCTCAC	30	2.1638043E-6	44.999996	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content