##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607341
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.709382373328985	31.0	31.0	34.0	30.0	34.0
2	31.75669187491047	31.0	31.0	34.0	30.0	34.0
3	31.77866799705602	33.0	31.0	34.0	30.0	34.0
4	35.530417014494326	37.0	35.0	37.0	33.0	37.0
5	33.674923971870825	37.0	35.0	37.0	32.0	37.0
6	34.49264745834712	37.0	35.0	37.0	30.0	37.0
7	35.51326190723169	37.0	35.0	37.0	32.0	37.0
8	35.39566240382256	37.0	35.0	37.0	33.0	37.0
9	37.27850746121207	39.0	37.0	39.0	34.0	39.0
10	36.86282500275792	39.0	37.0	39.0	32.0	39.0
11	36.84172318351634	39.0	37.0	39.0	32.0	39.0
12	36.92090604783804	39.0	37.0	39.0	33.0	39.0
13	36.84483016954232	39.0	37.0	39.0	33.0	39.0
14	37.97518198178618	40.0	37.0	41.0	33.0	41.0
15	37.913585613353945	40.0	37.0	41.0	33.0	41.0
16	38.05864086238209	40.0	37.0	41.0	33.0	41.0
17	37.90650227796246	40.0	37.0	41.0	33.0	41.0
18	37.8806403651326	39.0	37.0	41.0	33.0	41.0
19	37.77839796753389	39.0	37.0	41.0	33.0	41.0
20	37.57019532684274	39.0	36.0	41.0	32.0	41.0
21	37.739365529414286	39.0	36.0	41.0	33.0	41.0
22	37.817479801297786	40.0	36.0	41.0	33.0	41.0
23	37.76342120818453	40.0	36.0	41.0	33.0	41.0
24	37.74169371078192	40.0	36.0	41.0	33.0	41.0
25	37.65946807477183	39.0	36.0	41.0	33.0	41.0
26	37.5912987926058	39.0	36.0	41.0	33.0	41.0
27	37.56914155309785	40.0	36.0	41.0	33.0	41.0
28	37.50838820366153	40.0	36.0	41.0	33.0	41.0
29	37.45489436741468	40.0	36.0	41.0	33.0	41.0
30	37.4054427413924	39.0	36.0	41.0	33.0	41.0
31	37.33628554634053	40.0	36.0	41.0	32.0	41.0
32	37.13523045537845	40.0	36.0	41.0	32.0	41.0
33	37.0241495304944	40.0	36.0	41.0	31.0	41.0
34	36.87086332060572	40.0	36.0	41.0	31.0	41.0
35	36.74746970812114	40.0	36.0	41.0	31.0	41.0
36	36.62256952848564	40.0	35.0	41.0	30.0	41.0
37	36.58302996175131	39.0	35.0	41.0	30.0	41.0
38	36.45055084375993	39.0	35.0	41.0	30.0	41.0
39	36.426472113689016	39.0	35.0	41.0	30.0	41.0
40	36.286303740402836	39.0	35.0	41.0	30.0	41.0
41	36.21463560009945	39.0	35.0	41.0	29.0	41.0
42	36.26269756199565	39.0	35.0	41.0	30.0	41.0
43	36.16838316530582	39.0	35.0	41.0	30.0	41.0
44	36.157338299242106	39.0	35.0	41.0	29.0	41.0
45	36.15422308060875	39.0	35.0	41.0	29.0	41.0
46	35.983067173136675	39.0	35.0	40.0	28.0	41.0
47	35.80913687697686	39.0	35.0	40.0	28.0	41.0
48	35.79796028919503	39.0	35.0	40.0	27.0	41.0
49	35.75138184314907	39.0	35.0	40.0	27.0	41.0
50	35.554505623694105	39.0	35.0	40.0	26.0	41.0
51	34.27471881529487	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	8.0
11	6.0
12	1.0
13	8.0
14	10.0
15	14.0
16	25.0
17	64.0
18	154.0
19	495.0
20	1135.0
21	2134.0
22	3116.0
23	3717.0
24	4370.0
25	5379.0
26	6554.0
27	6870.0
28	7166.0
29	7731.0
30	9439.0
31	12679.0
32	16687.0
33	23574.0
34	34839.0
35	46417.0
36	48554.0
37	70414.0
38	120590.0
39	175116.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.53823305194282	17.546650069730184	18.620840680935423	14.29427619739158
2	27.32962207392552	35.17858995193804	22.18572432949529	15.30606364464115
3	25.718006852822384	34.902797604640554	25.709938897588007	13.66925664494905
4	23.384391964316585	21.986659882998183	38.10396465906303	16.5249834936222
5	29.382011094261706	29.053694711866974	23.922969139247968	17.64132505462335
6	21.270752345058213	37.64968938372348	26.731934777991277	14.347623493227035
7	71.42346721199459	3.9457240660518553	20.156057305533466	4.4747514164201005
8	69.70268761700594	11.460448084354589	12.387439675569409	6.449424623070072
9	64.91377990288815	5.528854465613222	13.076014957001092	16.481350674497524
10	36.35371232964677	26.691101045376485	21.711526144291263	15.243660480685481
11	27.31743781500014	22.84301570287532	29.86888749483404	19.970658987290502
12	21.087000548291652	19.874666785216213	34.563943484796845	24.47438918169529
13	21.728320663350573	24.133888540375175	38.72832560291501	15.409465193359251
14	19.16979094116814	32.09860687817881	28.928394427512714	19.803207753140327
15	15.508256481943421	25.621685346452814	39.77337278398791	19.096685387615853
16	17.802190202867912	27.185222140444985	29.361264923659032	25.65132273302807
17	17.895712622727594	28.07154465119266	31.15021050777076	22.88253221830899
18	18.92775228413692	25.63880916980741	31.753825281020053	23.679613265035623
19	18.25531291317398	27.973082666903764	28.7243904165864	25.047214003335856
20	21.380246023239007	27.230336828898427	33.56121190566749	17.82820524219508
21	19.157935986538043	31.95272507536952	30.9215086746984	17.967830263394042
22	17.788359422466126	23.085877620644744	32.96599439194785	26.159768564941277
23	18.320515163639538	29.692545044711288	31.28785970319804	20.699080088451133
24	20.6216935790602	26.378591269155223	29.398311656878096	23.601403494906485
25	16.506871757381766	35.062674839999275	27.056134856695003	21.374318545923956
26	18.066621551978212	27.56128764565541	30.533588214857883	23.8385025875085
27	22.39186881834093	29.10210244327322	26.5052087706906	22.000819967695247
28	15.545138563014845	28.990138982877824	33.02856220805116	22.43616024605617
29	18.41255571417046	27.937517803013463	29.535631548010095	24.114294934805983
30	18.98142888426765	32.76478946753142	29.348751360438367	18.905030287762557
31	19.77505223589384	25.986554505623694	28.637289430484685	25.60110382799778
32	21.52085895732381	29.408520090031793	26.348295273989407	22.72232567865499
33	23.628077142824214	24.653366066180286	25.26422553392575	26.454331257069754
34	19.749037196566675	26.255760767015563	28.236855407423505	25.758346628994254
35	19.012548140171667	26.605646580751174	29.326194016211648	25.055611262865508
36	23.470834341827736	28.797660622286326	27.79986860758618	19.931636428299754
37	17.533971854361884	26.99043864978653	31.685823944044618	23.789765551806973
38	21.6744794110722	24.548976604576342	29.20517468769604	24.57136929665542
39	23.921816574214485	22.667331861343136	27.086266199713176	26.324585364729202
40	22.765958497779664	23.527474680616	31.699490072298758	22.00707674930558
41	20.30177445619512	26.96771665341217	26.207188383461677	26.523320506931032
42	20.607533494363135	23.733125213018717	34.09830721127011	21.56103408134804
43	26.17261143245722	24.10770884890037	26.858387627379017	22.86129209126339
44	20.821251982000227	23.876043277170485	29.07855718616066	26.22414755466863
45	19.743932979989825	21.763720875093234	28.448598069288916	30.043748075628024
46	26.261194287887697	24.99337275105748	26.472278341162543	22.273154619892285
47	18.18945205411787	23.584608975847175	36.147073884358214	22.078865085676743
48	20.728223518583462	24.373128110896513	28.69903398584979	26.199614384670227
49	20.352487317668327	20.430367783502184	36.11941232355464	23.097732575274847
50	21.915036198774658	20.794084377639578	31.601686696600428	25.689192726985333
51	19.746238110056787	21.267129997810127	27.361235286272457	31.62539660586063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	312.0
2	466.0
3	2749.5
4	5033.0
5	3509.5
6	1986.0
7	1973.5
8	1961.0
9	2009.0
10	2057.0
11	2070.5
12	2084.0
13	2092.5
14	2101.0
15	2000.5
16	1900.0
17	1830.5
18	1761.0
19	1800.0
20	1839.0
21	1922.0
22	2005.0
23	2207.5
24	2410.0
25	2779.5
26	3888.5
27	4628.0
28	5643.0
29	6658.0
30	7293.0
31	7928.0
32	9506.5
33	11085.0
34	12985.5
35	14886.0
36	16129.5
37	17373.0
38	20021.0
39	22669.0
40	25095.5
41	27522.0
42	30576.0
43	33630.0
44	39455.5
45	45281.0
46	82225.5
47	119170.0
48	94598.0
49	70026.0
50	68370.5
51	66715.0
52	54348.0
53	41981.0
54	36184.0
55	30387.0
56	24966.0
57	19545.0
58	16797.5
59	14050.0
60	11697.0
61	9344.0
62	8123.0
63	6902.0
64	5149.5
65	3397.0
66	2728.5
67	2060.0
68	1607.5
69	1155.0
70	955.5
71	756.0
72	622.0
73	488.0
74	444.0
75	309.0
76	218.0
77	153.5
78	89.0
79	62.5
80	36.0
81	30.0
82	24.0
83	20.5
84	17.0
85	9.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	607341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.23069584041575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2095438456888	28.001036512152517
2	10.522380674515956	7.8351110881994455
3	4.0293940430923225	4.5005143211866
4	2.025731581753651	3.0167758549837784
5	1.2493106980521005	2.3256353304677613
6	0.9007846343658575	2.012210324387721
7	0.7031302393310777	1.8324619653713858
8	0.5780773954402707	1.7217778945485163
9	0.48633480481998737	1.6295924875374814
>10	4.17170580041324	29.395343834456217
>50	0.08063205527064427	1.9277048462332174
>100	0.03322749530109423	2.1673690149564826
>500	0.0035442661654500507	0.8400616737574859
>1k	0.003987299436131307	3.4757077536653727
>5k	8.860665413625127E-4	1.8127386205762361
>10k+	0.001329099812043769	7.505958477519763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	19204	3.1619798432840858	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	14096	2.320936673137496	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	12206	2.009744114097352	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	5518	0.9085505506791078	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	5472	0.9009765518876546	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT	3173	0.5224412644626331	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTT	2899	0.4773265760091942	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTC	2775	0.4569097097017985	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2707	0.4457133636622589	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCGCTGT	2616	0.43073001822699275	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTA	2251	0.3706319843382877	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	2055	0.3383601634007913	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	1572	0.2588331760905323	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	1024	0.16860379918365465	No Hit
GAATATGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	815	0.13419150032683452	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATG	727	0.1197021113344892	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGT	667	0.10982298247607192	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	628	0.1034015487181007	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6465214764028774E-4	0.0	0.0	0.27990865098848916	0.0
2	1.6465214764028774E-4	0.0	0.0	1.7907567577357695	0.0
3	1.6465214764028774E-4	0.0	0.0	2.2842192442137117	0.0
4	1.6465214764028774E-4	0.0	0.0	3.7581852698895677	0.0
5	1.6465214764028774E-4	0.0	0.0	8.321190237444862	0.0
6	1.6465214764028774E-4	0.0	0.0	9.845704472446286	0.0
7	1.6465214764028774E-4	0.0	0.0	11.349307884697394	0.0
8	1.6465214764028774E-4	0.0	0.0	13.324639699937926	0.0
9	1.6465214764028774E-4	0.0	0.0	13.937145689159797	0.0
10	1.6465214764028774E-4	0.0	0.0	17.309550977128172	0.0
11	1.6465214764028774E-4	0.0	0.0	18.997070838293478	0.0
12	1.6465214764028774E-4	0.0	0.0	22.883849435490113	0.0
13	1.6465214764028774E-4	0.0	0.0	23.67286252698237	0.0
14	1.6465214764028774E-4	0.0	0.0	24.052715031588516	0.0
15	1.6465214764028774E-4	0.0	0.0	24.83876438442325	0.0
16	1.6465214764028774E-4	0.0	0.0	25.753736368860327	0.0
17	1.6465214764028774E-4	0.0	0.0	26.867608147646873	0.0
18	1.6465214764028774E-4	0.0	0.0	27.982797143614544	0.0
19	1.6465214764028774E-4	0.0	0.0	29.175701953268426	0.0
20	1.6465214764028774E-4	0.0	0.0	29.900006750738054	0.0
21	1.6465214764028774E-4	0.0	0.0	30.706966926323105	0.0
22	1.6465214764028774E-4	0.0	0.0	31.58620939472224	0.0
23	1.6465214764028774E-4	0.0	0.0	32.4675923410407	0.0
24	3.293042952805755E-4	0.0	0.0	33.10578406529446	0.0
25	3.293042952805755E-4	0.0	0.0	33.65835667277526	0.0
26	3.293042952805755E-4	0.0	0.0	34.20681297656506	0.0
27	3.293042952805755E-4	0.0	0.0	34.769758669347205	0.0
28	3.293042952805755E-4	0.0	0.0	35.32529501548554	0.0
29	3.293042952805755E-4	0.0	0.0	35.937307048264486	0.0
30	3.293042952805755E-4	0.0	0.0	36.62209533030044	0.0
31	3.293042952805755E-4	0.0	0.0	37.22093519126817	0.0
32	4.939564429208632E-4	0.0	0.0	37.786515318412555	0.0
33	4.939564429208632E-4	0.0	0.0	38.317024538109564	0.0
34	4.939564429208632E-4	0.0	0.0	38.853131930826336	0.0
35	4.939564429208632E-4	0.0	0.0	39.39615471374401	0.0
36	4.939564429208632E-4	0.0	0.0	39.91563223954912	0.0
37	6.58608590561151E-4	0.0	0.0	40.45618524025218	0.0
38	6.58608590561151E-4	0.0	0.0	40.98620050350627	0.0
39	6.58608590561151E-4	0.0	0.0	41.518850201122596	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	70	0.0	45.000004	31
ACGCCCC	35	1.2106648E-7	45.000004	36
GCGCGAG	35	1.2106648E-7	45.000004	1
ATTGACG	35	1.2106648E-7	45.000004	1
ACGACGG	25	3.8886243E-5	45.000004	2
CTATGCG	20	7.030656E-4	45.0	1
TTAGGCG	20	7.030656E-4	45.0	1
GCGACAA	20	7.030656E-4	45.0	11
AAGCGAA	20	7.030656E-4	45.0	22
GCGCATT	20	7.030656E-4	45.0	11
TCGGACA	20	7.030656E-4	45.0	11
CTCGCGC	20	7.030656E-4	45.0	33
CTCGATG	20	7.030656E-4	45.0	1
TAAGCGA	20	7.030656E-4	45.0	21
AGGGCGC	40	6.8066583E-9	45.0	6
TCGCGCA	20	7.030656E-4	45.0	34
GTCGGAC	20	7.030656E-4	45.0	10
AGTCGAG	20	7.030656E-4	45.0	1
GTCGAAT	65	0.0	44.999996	43
CGCTCAC	30	2.1638043E-6	44.999996	34
>>END_MODULE