FastQCFastQC Report
Sat 14 Jan 2017
SRR2935900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931615
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53750.5769550726426689No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC25130.26974662280019107No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC16160.17346221346800986No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC13000.13954262222055247No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC12840.13782517456245338No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG12670.13600038642572307No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG12530.13449761972488636No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG207.032599E-445.01
TAGCGCG207.032599E-445.01
ATAGCGT207.032599E-445.014
ACTTCGA253.890234E-545.019
CTTACGG406.8121153E-945.02
CGTTTTT35100.042.9487151
CGCGAGG1050.042.8571432
GACACGA1800.041.25000425
CGGTCTA603.6379788E-1241.24999631
TACGGGA3750.039.6000024
CGTTATT2500.039.61
ACAACGA3050.039.09836213
ACACGAC1900.039.0789526
TGCACGG1100.038.8636362
ACGAAGG1350.038.3333362
CGACGGT659.094947E-1238.0769228
ACGGTCT659.094947E-1238.0769230
ATCGTTC301.1397559E-437.49999611
TCGACGG601.546141E-1037.4999962
AAGGGCG3600.036.8750045
Produced by FastQC (version 0.11.5)