##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935900.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 931615 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.990545450642163 33.0 31.0 34.0 30.0 34.0 2 32.046397921888335 33.0 31.0 34.0 30.0 34.0 3 32.03699274915067 33.0 31.0 34.0 30.0 34.0 4 35.74402730741777 37.0 35.0 37.0 33.0 37.0 5 33.77877127354111 37.0 35.0 37.0 32.0 37.0 6 34.70666101340146 37.0 35.0 37.0 32.0 37.0 7 35.76409460989787 37.0 35.0 37.0 33.0 37.0 8 35.796546856802436 37.0 35.0 37.0 35.0 37.0 9 37.68414312779421 39.0 38.0 39.0 35.0 39.0 10 37.12516758532227 39.0 37.0 39.0 33.0 39.0 11 37.15328542369971 39.0 37.0 39.0 34.0 39.0 12 37.21142424714072 39.0 37.0 39.0 34.0 39.0 13 37.2575516710229 39.0 37.0 39.0 34.0 39.0 14 38.38806910580014 40.0 38.0 41.0 34.0 41.0 15 38.49709375654106 40.0 38.0 41.0 34.0 41.0 16 38.447976900328996 40.0 38.0 41.0 34.0 41.0 17 38.42767022858155 40.0 38.0 41.0 34.0 41.0 18 38.338340408859885 40.0 38.0 41.0 34.0 41.0 19 38.202769384348684 40.0 37.0 41.0 34.0 41.0 20 38.25090729539563 40.0 37.0 41.0 34.0 41.0 21 38.13223166222098 40.0 37.0 41.0 34.0 41.0 22 38.20392329449397 40.0 37.0 41.0 34.0 41.0 23 38.18756782576494 40.0 37.0 41.0 34.0 41.0 24 38.14563634119244 40.0 37.0 41.0 34.0 41.0 25 37.94185151591591 40.0 36.0 41.0 34.0 41.0 26 38.004419207505244 40.0 37.0 41.0 34.0 41.0 27 38.00590587313429 40.0 37.0 41.0 34.0 41.0 28 37.90306403396253 40.0 36.0 41.0 34.0 41.0 29 37.884099118197966 40.0 36.0 41.0 34.0 41.0 30 37.73856367705544 40.0 36.0 41.0 33.0 41.0 31 37.66465439049393 40.0 36.0 41.0 33.0 41.0 32 37.54530573251826 40.0 36.0 41.0 33.0 41.0 33 37.415276696918795 40.0 36.0 41.0 33.0 41.0 34 37.2616295358061 40.0 36.0 41.0 32.0 41.0 35 37.16505208696726 40.0 36.0 41.0 32.0 41.0 36 37.05535333802053 40.0 36.0 41.0 31.0 41.0 37 37.02464966751287 40.0 36.0 41.0 31.0 41.0 38 36.935495886176156 40.0 36.0 41.0 31.0 41.0 39 36.8843631757754 40.0 35.0 41.0 31.0 41.0 40 36.82972687215212 40.0 35.0 41.0 31.0 41.0 41 36.71302308356993 40.0 35.0 41.0 31.0 41.0 42 36.705580094781645 40.0 35.0 41.0 31.0 41.0 43 36.64683909125551 39.0 35.0 41.0 31.0 41.0 44 36.5023448527557 39.0 35.0 41.0 30.0 41.0 45 36.4350928226789 39.0 35.0 41.0 30.0 41.0 46 36.33904348899492 39.0 35.0 41.0 30.0 41.0 47 36.28660337156443 39.0 35.0 41.0 30.0 41.0 48 36.207052269446066 39.0 35.0 41.0 30.0 41.0 49 36.11459884179624 39.0 35.0 41.0 29.0 41.0 50 36.01365800250103 39.0 35.0 41.0 29.0 41.0 51 34.86729281945868 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 5.0 9 16.0 10 21.0 11 22.0 12 16.0 13 31.0 14 30.0 15 41.0 16 53.0 17 124.0 18 233.0 19 410.0 20 744.0 21 1348.0 22 1975.0 23 2931.0 24 4596.0 25 7169.0 26 9810.0 27 10794.0 28 11369.0 29 11656.0 30 13331.0 31 16755.0 32 21353.0 33 29709.0 34 49870.0 35 63616.0 36 68269.0 37 105549.0 38 188415.0 39 311195.0 40 156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.05343945728654 19.65854993747417 23.754769942519175 15.533240662720116 2 34.681601305260216 23.989952931200122 23.83087434186869 17.49757142167097 3 31.070882285064112 23.906871400739576 27.97368011463963 17.048566199556685 4 27.22315548805032 25.064431122298377 28.121380613236152 19.591032776415148 5 23.636373394588965 33.22370292449134 25.005179178093957 18.13474450282574 6 24.398490792870444 34.273170784068526 26.675075004159442 14.653263418901584 7 79.31731455590561 5.533723694873956 10.76539128287973 4.38357046634071 8 80.96906984108242 4.8472813340274685 9.815642727950923 4.368006096939187 9 75.07597022375123 6.535317700981628 12.130547490111258 6.258164585155885 10 35.68491275902599 28.644987467999123 20.482173430011326 15.187926342963562 11 26.927969171814535 26.090069395619437 27.976685648041304 19.005275784524724 12 23.299002270251123 24.146777370480297 31.557671355656574 20.99654900361201 13 23.291273755789675 24.477708066100266 33.55635106776941 18.674667110340646 14 20.94287876429641 28.10120060325349 31.458488753401355 19.497431879048747 15 20.696210344401926 25.750122099794442 34.34863114054625 19.205036415257375 16 23.379185607788624 25.62227958974469 31.270857596754027 19.72767720571266 17 23.291703117704202 25.616268522941343 30.03805219967476 21.053976159679696 18 24.111569693489262 24.364249180187095 31.204199159524055 20.319981966799592 19 22.72312060239477 27.586288327259652 29.0289443600629 20.66164671028268 20 23.585171986281885 26.83576370067034 29.78022037000263 19.798843943045142 21 23.04868427408318 27.744078830847506 30.273235188355706 18.93400170671361 22 22.97773221770796 24.375627270922003 29.775604729421488 22.87103578194855 23 20.678391824949145 27.790235236658923 29.99747749875217 21.533895439639767 24 21.399183138957618 25.973604976304586 31.078074097132397 21.549137787605392 25 21.520477879810866 27.690193910574646 29.041503196062752 21.747825013551736 26 20.855610955169247 27.138785871846206 29.69198649656779 22.31361667641676 27 22.104088062128668 26.237125851344175 29.691879156089158 21.966906930438 28 20.72476291171782 26.79980464032889 30.136805440015458 22.338627007937827 29 20.867096386382787 25.629793423248877 29.87586073646302 23.627249453905318 30 22.319949764656 25.13130424048561 30.443155166028884 22.105590828829506 31 22.13360669375225 25.711479527487214 28.98933572344799 23.16557805531255 32 21.72217063915888 26.254944370796952 29.263805327307956 22.759079662736216 33 21.772942685551435 25.658775352479296 29.461741169903878 23.106540792065392 34 20.23926192686893 25.52943007572871 30.863929842263165 23.367378155139193 35 20.580067946522973 26.121198134422478 30.163640559673254 23.13509335938129 36 21.325332889659354 27.262012741314813 28.939851762799012 22.47280260622682 37 20.673668843889377 26.87773382781514 29.93747417119733 22.511123157098154 38 19.894591649984168 27.349387890920607 29.7432952453535 23.012725213741728 39 20.786269005973498 26.193545617019907 30.549207558916507 22.47097781809009 40 21.730757877449374 25.240791528689428 30.721059665205047 22.30739092865615 41 20.23282149815106 26.69944129280872 29.04772894382336 24.020008265216855 42 21.61085856281833 26.598648583374036 28.65497013251182 23.135522721295814 43 21.6358688943394 25.910488774869446 29.14830697230079 23.305335358490364 44 21.428057727709408 25.877320566972408 29.272929267991604 23.421692437326577 45 21.23860178292535 24.5514509749199 29.85772019557435 24.352227046580403 46 22.045587501274667 25.452144931114244 28.89498344273117 23.60728412487991 47 20.117430483622524 26.279203318967596 30.590318962232253 23.013047235177623 48 20.519313235617716 25.805187765332242 29.660106374414326 24.015392624635712 49 21.601949303091942 24.57152364442393 30.376282047841652 23.450245004642476 50 20.06268683952062 24.815615892831268 31.196255964105347 23.925441303542772 51 20.185591687553334 24.35233438705903 29.619316992534472 25.842756932853163 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 301.0 1 749.5 2 1198.0 3 5616.5 4 10035.0 5 6857.0 6 3679.0 7 3616.5 8 3554.0 9 3542.5 10 3531.0 11 3555.5 12 3580.0 13 3475.5 14 3371.0 15 3324.0 16 3277.0 17 3241.5 18 3206.0 19 3175.5 20 3145.0 21 3227.0 22 3309.0 23 3923.5 24 4538.0 25 5093.0 26 6591.5 27 7535.0 28 9133.0 29 10731.0 30 12538.5 31 14346.0 32 17208.0 33 20070.0 34 22932.5 35 25795.0 36 28718.0 37 31641.0 38 34593.0 39 37545.0 40 40930.0 41 44315.0 42 48654.0 43 52993.0 44 59202.0 45 65411.0 46 75942.5 47 86474.0 48 89429.0 49 92384.0 50 91082.5 51 89781.0 52 81641.5 53 73502.0 54 65905.0 55 58308.0 56 51280.5 57 44253.0 58 39830.0 59 35407.0 60 31870.0 61 28333.0 62 24405.0 63 20477.0 64 17384.0 65 14291.0 66 11848.0 67 9405.0 68 7793.0 69 6181.0 70 5080.0 71 3979.0 72 3252.0 73 2525.0 74 2094.0 75 1384.0 76 1105.0 77 776.5 78 448.0 79 299.5 80 151.0 81 128.5 82 106.0 83 84.5 84 63.0 85 36.0 86 9.0 87 9.5 88 10.0 89 5.5 90 1.0 91 2.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 931615.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.16412396119326 #Duplication Level Percentage of deduplicated Percentage of total 1 73.50572180083759 25.112585914608015 2 9.659707049913978 6.600308581641472 3 3.742744430526674 3.836027540387369 4 1.9952592967206644 2.7266514379155224 5 1.3243689878213845 2.262295313514491 6 1.0399799936208791 2.1318003251534816 7 0.8337987160569887 1.9940201885838318 8 0.6743936340834531 1.8432054170773358 9 0.6166774811961895 1.8961421320496739 >10 6.431591573977011 44.99613999638498 >50 0.14667500981492154 3.0278880850588568 >100 0.024656499821394864 1.4796512925177456 >500 0.002212762804467489 0.5156802366061459 >1k 0.0018966538324007053 0.997125785253308 >5k 3.161089720667842E-4 0.5804777532477559 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5375 0.5769550726426689 No Hit GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC 2513 0.26974662280019107 No Hit GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC 1616 0.17346221346800986 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC 1300 0.13954262222055247 No Hit CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC 1284 0.13782517456245338 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG 1267 0.13600038642572307 No Hit CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG 1253 0.13449761972488636 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.2202143589358265E-4 0.0 0.0 0.1058377119303575 0.0 2 3.2202143589358265E-4 0.0 0.0 0.5194205760963488 0.0 3 3.2202143589358265E-4 0.0 0.0 0.8361823285370029 0.0 4 3.2202143589358265E-4 0.0 0.0 1.2448275306859593 0.0 5 3.2202143589358265E-4 0.0 0.0 2.3153341240748593 0.0 6 3.2202143589358265E-4 0.0 0.0 3.264760657567772 0.0 7 3.2202143589358265E-4 0.0 0.0 4.015714646071607 0.0 8 3.2202143589358265E-4 0.0 0.0 5.206871937441969 0.0 9 3.2202143589358265E-4 0.0 0.0 5.734450389914288 0.0 10 3.2202143589358265E-4 0.0 0.0 6.766636432431852 0.0 11 3.2202143589358265E-4 0.0 0.0 7.790879279530707 0.0 12 5.367023931559711E-4 0.0 0.0 8.793224668988799 0.0 13 5.367023931559711E-4 0.0 0.0 9.228597650316923 0.0 14 5.367023931559711E-4 0.0 0.0 9.428250940570944 0.0 15 5.367023931559711E-4 0.0 0.0 9.708946292191516 0.0 16 5.367023931559711E-4 0.0 0.0 10.243394535296233 0.0 17 5.367023931559711E-4 0.0 0.0 10.886149321339824 0.0 18 5.367023931559711E-4 0.0 0.0 11.567009977297488 0.0 19 5.367023931559711E-4 0.0 0.0 12.013224346967363 0.0 20 7.513833504183595E-4 0.0 0.0 12.433999023201645 0.0 21 7.513833504183595E-4 0.0 0.0 12.926799160597458 0.0 22 7.513833504183595E-4 0.0 0.0 13.47455762305244 0.0 23 7.513833504183595E-4 0.0 0.0 14.037451092994424 0.0 24 7.513833504183595E-4 0.0 0.0 14.490213231860801 0.0 25 7.513833504183595E-4 0.0 0.0 14.903903436505423 0.0 26 7.513833504183595E-4 0.0 0.0 15.305142145628828 0.0 27 7.513833504183595E-4 0.0 0.0 15.70906436671801 0.0 28 7.513833504183595E-4 0.0 0.0 16.115884780730237 0.0 29 7.513833504183595E-4 0.0 0.0 16.523456578092883 0.0 30 7.513833504183595E-4 0.0 0.0 17.034611937334628 0.0 31 8.587238290495538E-4 0.0 0.0 17.488125459551423 0.0 32 8.587238290495538E-4 0.0 0.0 17.91845343838388 0.0 33 8.587238290495538E-4 0.0 0.0 18.349962162481283 0.0 34 9.660643076807479E-4 0.0 0.0 18.783295674715415 0.0 35 9.660643076807479E-4 0.0 0.0 19.219527379872588 0.0 36 9.660643076807479E-4 0.0 0.0 19.63686716079067 0.0 37 0.0010734047863119422 0.0 0.0 20.053240877401073 0.0 38 0.0010734047863119422 0.0 0.0 20.500206630421363 0.0 39 0.0010734047863119422 0.0 0.0 20.964024838586756 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCGCG 20 7.032599E-4 45.0 1 TAGCGCG 20 7.032599E-4 45.0 1 ATAGCGT 20 7.032599E-4 45.0 14 ACTTCGA 25 3.890234E-5 45.0 19 CTTACGG 40 6.8121153E-9 45.0 2 CGTTTTT 3510 0.0 42.948715 1 CGCGAGG 105 0.0 42.857143 2 GACACGA 180 0.0 41.250004 25 CGGTCTA 60 3.6379788E-12 41.249996 31 TACGGGA 375 0.0 39.600002 4 CGTTATT 250 0.0 39.6 1 ACAACGA 305 0.0 39.098362 13 ACACGAC 190 0.0 39.07895 26 TGCACGG 110 0.0 38.863636 2 ACGAAGG 135 0.0 38.333336 2 CGACGGT 65 9.094947E-12 38.07692 28 ACGGTCT 65 9.094947E-12 38.07692 30 ATCGTTC 30 1.1397559E-4 37.499996 11 TCGACGG 60 1.546141E-10 37.499996 2 AAGGGCG 360 0.0 36.875004 5 >>END_MODULE