##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935897.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407119
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.761096878308308	31.0	31.0	34.0	30.0	34.0
2	31.82428970399318	33.0	31.0	34.0	30.0	34.0
3	31.84677207401276	33.0	31.0	34.0	30.0	34.0
4	35.582665019318675	37.0	35.0	37.0	33.0	37.0
5	33.46303169343607	37.0	35.0	37.0	31.0	37.0
6	34.46171266877743	37.0	35.0	37.0	30.0	37.0
7	35.60458244395373	37.0	35.0	37.0	32.0	37.0
8	35.61348156190205	37.0	35.0	37.0	33.0	37.0
9	37.507107258565675	39.0	37.0	39.0	35.0	39.0
10	36.9812020564012	39.0	37.0	39.0	33.0	39.0
11	37.04526440672138	39.0	37.0	39.0	33.0	39.0
12	36.95163330623233	39.0	35.0	39.0	33.0	39.0
13	36.81629695494438	39.0	35.0	39.0	33.0	39.0
14	37.8091196922767	40.0	37.0	41.0	33.0	41.0
15	37.93112333249001	40.0	37.0	41.0	33.0	41.0
16	37.98770629717601	40.0	36.0	41.0	33.0	41.0
17	37.97841663002709	40.0	36.0	41.0	33.0	41.0
18	37.95899724650532	40.0	36.0	41.0	33.0	41.0
19	37.878566217739774	40.0	36.0	41.0	33.0	41.0
20	37.89880354392696	40.0	36.0	41.0	34.0	41.0
21	37.798439768225016	39.0	36.0	41.0	33.0	41.0
22	37.88460130821701	39.0	36.0	41.0	33.0	41.0
23	37.87996138721111	40.0	36.0	41.0	34.0	41.0
24	37.816576971352355	39.0	36.0	41.0	33.0	41.0
25	37.649272080153466	39.0	35.0	41.0	33.0	41.0
26	37.71656198801824	39.0	36.0	41.0	33.0	41.0
27	37.679609647302144	39.0	36.0	41.0	33.0	41.0
28	37.6212286825228	39.0	36.0	41.0	33.0	41.0
29	37.658785269171915	40.0	36.0	41.0	33.0	41.0
30	37.498790279991844	39.0	36.0	41.0	33.0	41.0
31	37.47426673773516	39.0	36.0	41.0	33.0	41.0
32	37.433050287508074	40.0	35.0	41.0	33.0	41.0
33	37.364129406881034	40.0	35.0	41.0	33.0	41.0
34	37.298298531878885	40.0	35.0	41.0	33.0	41.0
35	37.2375276024946	40.0	35.0	41.0	32.0	41.0
36	37.14004996082227	39.0	35.0	41.0	32.0	41.0
37	37.05993579272891	39.0	35.0	41.0	32.0	41.0
38	36.989590267219164	39.0	35.0	41.0	32.0	41.0
39	36.99113527003161	39.0	35.0	41.0	32.0	41.0
40	36.902355330996585	39.0	35.0	41.0	31.0	41.0
41	36.786433450661846	39.0	35.0	41.0	31.0	41.0
42	36.82214045524773	39.0	35.0	41.0	31.0	41.0
43	36.742141732515556	39.0	35.0	41.0	31.0	41.0
44	36.60346974717466	39.0	35.0	41.0	31.0	41.0
45	36.57214475374522	39.0	35.0	41.0	31.0	41.0
46	36.48935077950182	39.0	35.0	41.0	31.0	41.0
47	36.41159710060204	39.0	35.0	40.0	31.0	41.0
48	36.36174926741322	39.0	35.0	40.0	31.0	41.0
49	36.29551556178906	39.0	35.0	40.0	30.0	41.0
50	36.14926593944277	39.0	35.0	40.0	30.0	41.0
51	34.8717549414299	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	6.0
11	6.0
12	5.0
13	3.0
14	9.0
15	10.0
16	24.0
17	28.0
18	77.0
19	179.0
20	315.0
21	495.0
22	791.0
23	1172.0
24	1712.0
25	2349.0
26	3143.0
27	3938.0
28	4302.0
29	5293.0
30	6509.0
31	8324.0
32	11052.0
33	15958.0
34	26791.0
35	36354.0
36	31422.0
37	47042.0
38	81653.0
39	118086.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15293808444214	19.46777232209747	23.003593543902397	13.375696049557991
2	30.929777288704287	25.708699422036307	25.488125093645834	17.873398195613568
3	30.8543693612924	25.667433846123615	27.010038833854477	16.468157958729513
4	27.53077110132418	24.89026549976788	28.498301479420025	19.080661919487916
5	23.964491954440838	33.191278225776735	24.58937067540449	18.25485914437793
6	23.304488368265787	35.933719624974515	25.49966962976427	15.262122376995425
7	76.64049086385062	5.605240728140912	12.569052291836048	5.1852161161724215
8	76.40763511405756	6.425639677833754	10.311481409612423	6.8552437984962635
9	71.71367585398863	6.223978738403268	12.840226076405179	9.222119331202915
10	38.15370935770622	24.145028849058875	20.638683038620158	17.062578754614744
11	29.685914929050227	25.10690977330952	25.54216334781722	19.665011949823025
12	26.785534450615177	21.66393609730816	30.640427000459326	20.91010245161734
13	23.136969780334496	25.31029011173637	33.18955882678038	18.363181281148755
14	19.309587614432143	29.639491156148445	29.623770936753136	21.427150292666273
15	15.578246163898024	25.37292536088957	39.338620894627866	19.710207580584544
16	17.899680437415107	24.950198836212508	33.430520314699145	23.719600411673245
17	18.661865449659683	24.293879676458236	30.182575610570865	26.861679263311217
18	19.563567409037653	24.44199361857344	32.99330171276703	23.001137259621878
19	20.833957638921298	26.855538552609925	29.350632124759592	22.959871683709185
20	22.042449504935906	26.258907100872225	31.036625654906796	20.662017739285073
21	20.094861698913586	28.959591667301204	30.15457396977296	20.79097266401224
22	18.61666981889816	25.239057867601367	29.508079947140764	26.63619236635971
23	18.174293019976957	28.213126874451945	29.314770374264036	24.297809731307062
24	20.853116656309332	23.911681842409713	30.867878924835246	24.36732257644571
25	17.40301975589447	27.527823560187564	29.16542829000857	25.903728393909397
26	17.31164598065922	27.89577494540908	29.592821754818615	25.199757319113086
27	19.5770769725805	29.610752630066393	28.45728153193538	22.354888865417728
28	16.27435712899668	25.66252127756258	33.6980096728475	24.36511192059324
29	17.79799026820168	23.633876090283184	31.581920765181682	26.986212876333454
30	19.700382443462477	28.05985473534765	28.743438650615666	23.496324170574205
31	19.109891702426072	26.27364480655533	29.34056135920947	25.275902131809126
32	20.13661853168238	26.22476474937303	28.331765405200937	25.30685131374365
33	20.26827536911812	25.36604776490412	27.38265715920898	26.983019706768786
34	17.838518958830218	23.22588727128923	32.61970087370032	26.31589289618023
35	16.90758721651409	23.853959161817553	29.102547412427327	30.135906209241032
36	21.639373254502985	23.85494167552976	29.39681026923332	25.108874800733936
37	18.21604985274576	25.546584659522154	34.44152692456014	21.795838563171944
38	18.112149027679866	25.168808137178566	29.534853445798404	27.184189389343167
39	20.79318331986471	22.102382841380532	32.01447242698081	25.089961411773952
40	20.045981641731288	21.68506014212061	30.88384477265861	27.385113443489495
41	18.890054259319758	23.62773537958189	28.647889192103538	28.834321168994816
42	20.84844971617635	22.274813997872858	30.555685192781475	26.321051093169316
43	22.96748616497879	22.549917837290817	29.61787585447989	24.864720143250498
44	19.76670211903645	23.302277712413325	30.375885183447593	26.555134985102637
45	18.433431011571557	22.36225771825928	29.90673488586875	29.29757638430041
46	22.641782869382173	23.764059157150612	29.217992773611645	24.37616519985557
47	17.22886920040578	23.050754202088335	34.05859220522746	25.661784392278424
48	19.54981221706675	22.31779897278191	30.11576467814079	28.01662413201054
49	20.482708986807296	19.459666583971764	34.53044441551487	25.52718001370607
50	20.23192236176646	20.160444489203403	32.3148759944881	27.292757154542034
51	18.382585926964843	20.234133017618927	28.37401349482584	33.009267560590395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	344.5
2	487.0
3	1269.0
4	2051.0
5	1431.0
6	811.0
7	799.0
8	787.0
9	846.5
10	906.0
11	919.5
12	933.0
13	918.0
14	903.0
15	865.5
16	828.0
17	801.0
18	774.0
19	836.0
20	898.0
21	995.0
22	1092.0
23	1070.5
24	1049.0
25	1291.0
26	1899.0
27	2265.0
28	3030.0
29	3795.0
30	4193.5
31	4592.0
32	5359.0
33	6126.0
34	7488.0
35	8850.0
36	9499.5
37	10149.0
38	11547.5
39	12946.0
40	15616.0
41	18286.0
42	21471.5
43	24657.0
44	28957.5
45	33258.0
46	43623.0
47	53988.0
48	54066.0
49	54144.0
50	52893.0
51	51642.0
52	43026.5
53	34411.0
54	28232.0
55	22053.0
56	18428.0
57	14803.0
58	12853.0
59	10903.0
60	10127.5
61	9352.0
62	8220.5
63	7089.0
64	5895.0
65	4701.0
66	3513.5
67	2326.0
68	1841.0
69	1356.0
70	1135.0
71	914.0
72	797.5
73	681.0
74	500.5
75	215.0
76	110.0
77	86.0
78	62.0
79	59.5
80	57.0
81	40.5
82	24.0
83	13.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.29003164455012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.8274704665229	23.245587014894358
2	12.882019269360049	8.576856582454008
3	5.237931860784018	5.231127521924917
4	2.661878723232543	3.5445610772146403
5	1.576607345577716	2.624265421265617
6	0.92372245201273	1.8450449794971124
7	0.7045596936445465	1.6418370147831045
8	0.4737055301181642	1.2615737670265659
9	0.3790592738289738	1.1357005698834053
>10	5.09291850648481	39.65584087659977
>50	0.18175757567585105	3.6820817164597703
>100	0.049503079553991955	2.9080328746629167
>500	0.003694259668208355	0.7194448243583719
>1k	0.005171963535491697	3.9280457589754527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	3363	0.8260484035380319	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2970	0.7295164313136945	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	2652	0.6514065911932383	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	2568	0.6307738032368915	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2009	0.4934675119559638	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	1314	0.32275575445999816	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1094	0.2687175002886134	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	792	0.19453771501698522	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	551	0.1353412638565137	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	548	0.13460437857235846	No Hit
ACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	522	0.12821803944301297	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT	512	0.1257617551624955	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	439	0.10783087991471782	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	437	0.10733962305861432	No Hit
CGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	430	0.10562022406225206	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA	426	0.10463771035004507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.45628428051749E-4	0.0	0.0	0.3438797992724486	0.0
2	2.45628428051749E-4	0.0	0.0	1.9972047484887712	0.0
3	2.45628428051749E-4	0.0	0.0	2.641242486840457	0.0
4	2.45628428051749E-4	0.0	0.0	3.5431900746464793	0.0
5	2.45628428051749E-4	0.0	0.0	6.200152780882248	0.0
6	2.45628428051749E-4	0.0	0.0	7.586725257234371	0.0
7	2.45628428051749E-4	0.0	0.0	9.034950469027484	0.0
8	2.45628428051749E-4	0.0	0.0	11.395439662604792	0.0
9	2.45628428051749E-4	0.0	0.0	12.224435607279444	0.0
10	2.45628428051749E-4	0.0	0.0	14.741390109525716	0.0
11	2.45628428051749E-4	0.0	0.0	17.107528756948213	0.0
12	2.45628428051749E-4	0.0	0.0	19.788071792276952	0.0
13	2.45628428051749E-4	0.0	0.0	20.5814516148841	0.0
14	2.45628428051749E-4	0.0	0.0	20.888732778376838	0.0
15	2.45628428051749E-4	0.0	0.0	21.523191008034505	0.0
16	2.45628428051749E-4	0.0	0.0	22.718173310506266	0.0
17	2.45628428051749E-4	0.0	0.0	24.132501799228237	0.0
18	2.45628428051749E-4	0.0	0.0	25.78312483573599	0.0
19	4.91256856103498E-4	0.0	0.0	26.79290330345673	0.0
20	4.91256856103498E-4	0.0	0.0	27.63565444010228	0.0
21	4.91256856103498E-4	0.0	0.0	28.705366244267648	0.0
22	7.36885284155247E-4	0.0	0.0	29.748058921347322	0.0
23	7.36885284155247E-4	0.0	0.0	30.818016353940738	0.0
24	7.36885284155247E-4	0.0	0.0	31.58928961802323	0.0
25	7.36885284155247E-4	0.0	0.0	32.28564621154994	0.0
26	7.36885284155247E-4	0.0	0.0	32.95301865056654	0.0
27	7.36885284155247E-4	0.0	0.0	33.59091567821694	0.0
28	7.36885284155247E-4	0.0	0.0	34.22512827944655	0.0
29	7.36885284155247E-4	0.0	0.0	34.86965727465434	0.0
30	7.36885284155247E-4	0.0	0.0	35.58173408757636	0.0
31	7.36885284155247E-4	0.0	0.0	36.34760352624171	0.0
32	7.36885284155247E-4	0.0	0.0	36.97395601777367	0.0
33	7.36885284155247E-4	0.0	0.0	37.61087053171186	0.0
34	7.36885284155247E-4	0.0	0.0	38.23599488110356	0.0
35	7.36885284155247E-4	0.0	0.0	38.8802782478833	0.0
36	7.36885284155247E-4	0.0	0.0	39.50147254242617	0.0
37	7.36885284155247E-4	0.0	0.0	40.10620973228958	0.0
38	7.36885284155247E-4	0.0	0.0	40.67901522650625	0.0
39	7.36885284155247E-4	0.0	0.0	41.3171578825847	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCA	35	1.2093369E-7	45.000004	18
ACACGAC	35	1.2093369E-7	45.000004	26
GTCGATG	30	2.1620344E-6	45.000004	1
ACGTTGG	30	2.1620344E-6	45.000004	1
TGGACGT	30	2.1620344E-6	45.000004	12
GCTTGCG	30	2.1620344E-6	45.000004	1
ACGATAG	60	0.0	45.000004	1
CGATGTA	30	2.1620344E-6	45.000004	10
CACGTGA	35	1.2093369E-7	45.000004	43
GTAAGCG	30	2.1620344E-6	45.000004	1
AGCCGCG	20	7.0279144E-4	45.0	14
TTGTGCG	45	3.8380676E-10	45.0	1
GGTCGGC	20	7.0279144E-4	45.0	22
GGGTCGT	45	3.8380676E-10	45.0	7
AGCCCGC	20	7.0279144E-4	45.0	23
TAGGTGA	20	7.0279144E-4	45.0	3
ATAGCGG	40	6.7957444E-9	45.0	2
ATCCGTG	20	7.0279144E-4	45.0	1
TGTTGCG	50	2.1827873E-11	45.0	1
CTAACCG	20	7.0279144E-4	45.0	41
>>END_MODULE