FastQCFastQC Report
Sat 14 Jan 2017
SRR2935896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088801
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67800.6227033222783595No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC60190.5528099257807441No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC54940.5045917481706942No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG46810.4299224559859882No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC32680.3001466751040824TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG20970.19259717799671383No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC19940.18313723077036118TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT15410.1415318318039752TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT14620.13427614412551053No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT12270.11269276938577388No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG207.0331234E-445.01
ATTAACG302.165396E-644.9999961
CGTTTTT32600.043.1365051
TACGGGT1400.041.7857174
CGTTAGG1550.040.6451642
TCGTTAG501.0822987E-940.5000041
CGTAAGG1450.040.344832
GTTTACG451.9281288E-840.01
TTAGCCG659.094947E-1238.076921
ATAACGG659.094947E-1238.076922
CGTTATT2800.037.767861
TAGCACG601.5643309E-1037.4999961
CGCAATA301.1398825E-437.49999615
CGACTAA301.1398825E-437.49999635
GCGTTAG850.037.0588231
ACGGGTC1700.037.0588235
GTACGAG552.748493E-936.818181
TACGCGG800.036.56252
GCGTAAG800.036.56251
ACGGGCG2350.036.382985
Produced by FastQC (version 0.11.5)