##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935896.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088801
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.889285553558455	33.0	31.0	34.0	30.0	34.0
2	31.938266037595483	33.0	31.0	34.0	30.0	34.0
3	31.944445311861397	33.0	31.0	34.0	30.0	34.0
4	35.667489284084056	37.0	35.0	37.0	33.0	37.0
5	33.58490027103208	37.0	35.0	37.0	32.0	37.0
6	34.56822688443526	37.0	35.0	37.0	30.0	37.0
7	35.67799074394678	37.0	35.0	37.0	32.0	37.0
8	35.69021060781539	37.0	35.0	37.0	35.0	37.0
9	37.56697504870036	39.0	37.0	39.0	35.0	39.0
10	37.07838163263994	39.0	37.0	39.0	33.0	39.0
11	37.118562528873504	39.0	37.0	39.0	34.0	39.0
12	37.09778738263466	39.0	37.0	39.0	34.0	39.0
13	37.051478644857966	39.0	37.0	39.0	33.0	39.0
14	38.06533792676532	40.0	37.0	41.0	33.0	41.0
15	38.17675406249627	40.0	37.0	41.0	33.0	41.0
16	38.216571255904434	40.0	37.0	41.0	33.0	41.0
17	38.21538462951448	40.0	37.0	41.0	34.0	41.0
18	38.17202317044161	40.0	37.0	41.0	34.0	41.0
19	38.052512809962515	40.0	37.0	41.0	34.0	41.0
20	38.03559787325691	40.0	36.0	41.0	34.0	41.0
21	37.93370413877283	40.0	36.0	41.0	33.0	41.0
22	38.03646028980502	40.0	36.0	41.0	34.0	41.0
23	38.0375229265954	40.0	36.0	41.0	34.0	41.0
24	37.98456099875	40.0	36.0	41.0	34.0	41.0
25	37.78987069262427	40.0	36.0	41.0	33.0	41.0
26	37.846422808208295	40.0	36.0	41.0	33.0	41.0
27	37.82329461490208	40.0	36.0	41.0	33.0	41.0
28	37.718583101962615	40.0	36.0	41.0	33.0	41.0
29	37.673465582783265	40.0	36.0	41.0	33.0	41.0
30	37.48435113487221	40.0	36.0	41.0	33.0	41.0
31	37.40159955767859	40.0	35.0	41.0	33.0	41.0
32	37.25010355427668	40.0	35.0	41.0	32.0	41.0
33	37.12709668708975	39.0	35.0	41.0	32.0	41.0
34	37.004307490533165	39.0	35.0	41.0	31.0	41.0
35	36.85260116403273	39.0	35.0	41.0	31.0	41.0
36	36.78141735725812	39.0	35.0	41.0	31.0	41.0
37	36.6783875106654	39.0	35.0	41.0	31.0	41.0
38	36.5894759464769	39.0	35.0	41.0	30.0	41.0
39	36.52034210108183	39.0	35.0	41.0	30.0	41.0
40	36.48301204719687	39.0	35.0	41.0	30.0	41.0
41	36.38938061225146	39.0	35.0	41.0	30.0	41.0
42	36.41916015874342	39.0	35.0	41.0	30.0	41.0
43	36.36802133723242	39.0	35.0	41.0	30.0	41.0
44	36.2256114753752	39.0	35.0	41.0	30.0	41.0
45	36.163734236100076	39.0	35.0	41.0	30.0	41.0
46	36.06030945967169	39.0	35.0	40.0	29.0	41.0
47	35.992368669756914	38.0	35.0	40.0	29.0	41.0
48	35.894426070512424	38.0	35.0	40.0	29.0	41.0
49	35.828227564081956	38.0	35.0	40.0	29.0	41.0
50	35.713327779823864	38.0	35.0	40.0	28.0	41.0
51	34.46964137615598	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	4.0
9	21.0
10	19.0
11	13.0
12	5.0
13	26.0
14	27.0
15	33.0
16	77.0
17	143.0
18	240.0
19	507.0
20	987.0
21	1547.0
22	2462.0
23	3577.0
24	5484.0
25	7893.0
26	10696.0
27	12216.0
28	13259.0
29	14876.0
30	17613.0
31	22397.0
32	29348.0
33	41440.0
34	68040.0
35	82146.0
36	88323.0
37	134022.0
38	222020.0
39	309187.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71652854837569	19.191569441982512	23.03983923600364	14.052062773638157
2	32.99298953619624	25.076666902399978	24.514305185245053	17.41603837615873
3	31.02008539668865	25.128558845923177	27.490147419041676	16.361208338346493
4	27.360187949864116	24.722791400816128	29.31894809060609	18.598072558713667
5	24.02018366992683	32.789922125347054	24.890498814751272	18.299395389974844
6	24.17585950049642	34.792767457046786	26.061970920305917	14.96940212215088
7	77.48844830230685	5.273139903435063	12.27542957804043	4.9629822162176565
8	77.92838177040616	5.910538289366009	10.772216410528646	5.388863529699183
9	72.49387169923614	6.301335138376986	12.81629976460345	8.388493397783433
10	39.05194796845337	23.373417180917357	20.941384146414265	16.633250704215
11	28.79874283730452	24.165572955939606	26.58979923787726	20.445884968878612
12	25.480597464550453	21.499337344473414	31.432465620439366	21.587599570536764
13	23.845404256608877	22.473252688048596	34.85255799728325	18.828785058059278
14	20.61735799287473	26.139946601812454	32.75336815451125	20.48932725080157
15	18.73501218312621	24.58823972424713	36.577758470096924	20.098989622529736
16	21.931831436598607	24.841729572254252	31.08906035170798	22.137378639439163
17	22.315556286226776	24.282306867829842	29.98885930486838	23.413277541074997
18	23.36652886983021	23.833740049834635	31.474346551849237	21.325384528485923
19	22.865427199277004	26.269079473659556	28.820601744487746	22.044891582575694
20	22.704516252281177	25.981515446808	30.575467877050077	20.738500423860742
21	22.179535103292523	27.153263084806127	30.930812884999188	19.736388926902162
22	21.2509907687447	23.492630884798967	30.776422872499197	24.479955473957133
23	19.92788397512493	26.152529250064983	30.665842518513486	23.253744256296606
24	20.842927219941938	24.345587485683794	30.93283345625142	23.878651838122853
25	21.411993559888355	27.477289238345666	28.24859639181081	22.862120809955172
26	20.764308629400595	25.403540224522203	29.836581707768456	23.995569438308745
27	21.668330576478162	25.76549801111498	29.29598705364892	23.270184358757938
28	20.39132954506838	25.30039924651061	30.430078591037297	23.87819261738371
29	22.70433256398552	24.38351911873703	29.59778692341392	23.314361393863525
30	22.8779180033817	26.03496874084429	29.36294143741602	21.72417181835799
31	24.20414749802765	25.1904618015597	27.332818393811177	23.272572306601482
32	24.59512803533428	26.008793158713118	27.783956847945586	21.61212195800702
33	22.253653330590254	25.253742419413648	28.659047888457117	23.833556361538978
34	22.73767198964733	23.65822588333405	29.551497472908274	24.052604654110347
35	21.09458018499248	26.747403795551257	28.731053700354792	23.42696231910147
36	23.886274902392632	26.111658604281224	27.220401156868885	22.78166533645726
37	21.46599791881161	26.685317151619074	28.855043299923494	22.993641629645822
38	21.566934637275313	26.56242968182432	28.509709304087703	23.36092637681266
39	21.287361051284854	23.798563741216256	29.731052781913313	25.183022425585577
40	21.86698946823157	24.16603217667875	30.249696684701792	23.717281670387884
41	20.19726286070641	25.669061655894883	27.280834606140147	26.85284087725856
42	21.397665872827083	25.59246363660577	28.84356278144491	24.166307709122236
43	21.585027934397562	24.422001816677245	29.31132502633631	24.681645222588884
44	22.19955712751917	23.96709775248186	29.299568975414243	24.53377614458473
45	21.73804028468012	22.63416363504442	29.289649807448743	26.338146272826712
46	22.951209633348977	24.676134573719164	28.46617517801692	23.906480614914937
47	20.1731996939753	24.257233415472616	31.319405474462275	24.250161416089806
48	20.508798210141247	24.84393383180214	29.7268279511132	24.920440006943416
49	21.49584726685593	22.744835833177966	31.33217181101046	24.427145088955648
50	20.834202025898215	22.592374547782377	30.98858285398342	25.58484057233599
51	20.05536365231112	22.815831359449522	28.885535557002612	28.243269431236744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	820.5
2	1276.0
3	6247.0
4	11218.0
5	7416.0
6	3614.0
7	3552.0
8	3490.0
9	3513.0
10	3536.0
11	3505.0
12	3474.0
13	3398.0
14	3322.0
15	3169.5
16	3017.0
17	2954.0
18	2891.0
19	3078.0
20	3265.0
21	3438.5
22	3612.0
23	3888.5
24	4165.0
25	5023.0
26	6685.5
27	7490.0
28	8528.5
29	9567.0
30	11103.0
31	12639.0
32	15633.0
33	18627.0
34	21830.5
35	25034.0
36	27810.0
37	30586.0
38	33876.5
39	37167.0
40	41039.0
41	44911.0
42	51079.5
43	57248.0
44	64074.0
45	70900.0
46	93602.0
47	116304.0
48	116817.5
49	117331.0
50	115697.5
51	114064.0
52	100037.5
53	86011.0
54	75616.0
55	65221.0
56	58885.0
57	52549.0
58	48576.0
59	44603.0
60	40110.5
61	35618.0
62	31354.5
63	27091.0
64	24037.0
65	20983.0
66	17458.0
67	13933.0
68	12066.5
69	10200.0
70	8471.5
71	6743.0
72	5755.5
73	4768.0
74	3876.5
75	2388.0
76	1791.0
77	1283.5
78	776.0
79	567.0
80	358.0
81	211.5
82	65.0
83	58.5
84	52.0
85	45.5
86	39.0
87	23.5
88	8.0
89	8.5
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1088801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.07308855065386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30196474230117	25.316974241519887
2	10.793195534121715	7.355150143573019
3	3.9860896491975115	4.074551567638548
4	2.0276275155000056	2.7635012753349586
5	1.1429208721836317	1.9471422042151711
6	0.7553008885177354	1.5441260434111401
7	0.5544831197954018	1.3225066708442075
8	0.4229871989112741	1.1529984227437502
9	0.3663075787515209	1.1233107510818565
>10	5.4452420636284735	43.745103458608696
>50	0.16218480978613736	3.361801695296427
>100	0.03519794512965202	2.0755317052649747
>500	0.0032490410878983603	0.832131566273263
>1k	0.0024367808159237703	1.6975668460082387
>5k	8.122602719745901E-4	1.6876034081858984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6780	0.6227033222783595	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	6019	0.5528099257807441	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	5494	0.5045917481706942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	4681	0.4299224559859882	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	3268	0.3001466751040824	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2097	0.19259717799671383	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1994	0.18313723077036118	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	1541	0.1415318318039752	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1462	0.13427614412551053	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT	1227	0.11269276938577388	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.673765913146663E-4	0.0	0.0	0.15255312954341518	0.0
2	3.673765913146663E-4	0.0	0.0	0.7849919314916133	0.0
3	3.673765913146663E-4	0.0	0.0	1.2170268028776607	0.0
4	3.673765913146663E-4	0.0	0.0	1.811442127624791	0.0
5	3.673765913146663E-4	0.0	0.0	3.5394897690211526	0.0
6	3.673765913146663E-4	0.0	0.0	4.869760406171559	0.0
7	3.673765913146663E-4	0.0	0.0	5.99586150269884	0.0
8	3.673765913146663E-4	0.0	0.0	7.64345367059729	0.0
9	3.673765913146663E-4	0.0	0.0	8.323375896972909	0.0
10	3.673765913146663E-4	0.0	0.0	10.048392681490924	0.0
11	3.673765913146663E-4	0.0	0.0	11.620580804022039	0.0
12	3.673765913146663E-4	0.0	0.0	13.406031037811317	0.0
13	3.673765913146663E-4	0.0	0.0	14.01817228308938	0.0
14	3.673765913146663E-4	0.0	0.0	14.305919998236593	0.0
15	3.673765913146663E-4	0.0	0.0	14.752098868388254	0.0
16	3.673765913146663E-4	0.0	0.0	15.547009967845364	0.0
17	3.673765913146663E-4	0.0	0.0	16.49778058616772	0.0
18	3.673765913146663E-4	0.0	0.0	17.471879618038557	0.0
19	3.673765913146663E-4	0.0	0.0	18.15970044112744	0.0
20	3.673765913146663E-4	0.0	0.0	18.780291348005743	0.0
21	3.673765913146663E-4	0.0	0.0	19.50163528505209	0.0
22	3.673765913146663E-4	0.0	0.0	20.268533919421454	0.0
23	3.673765913146663E-4	0.0	0.0	21.05343400676524	0.0
24	3.673765913146663E-4	0.0	0.0	21.675769952452285	0.0
25	3.673765913146663E-4	0.0	0.0	22.22839618993737	0.0
26	3.673765913146663E-4	0.0	0.0	22.75209152085643	0.0
27	3.673765913146663E-4	0.0	0.0	23.311697913576495	0.0
28	3.673765913146663E-4	0.0	0.0	23.886642278983945	0.0
29	3.673765913146663E-4	0.0	0.0	24.465995163487175	0.0
30	3.673765913146663E-4	0.0	0.0	25.181644763368144	0.0
31	3.673765913146663E-4	0.0	0.0	25.769447309471612	0.0
32	3.673765913146663E-4	0.0	0.0	26.340258688226776	0.0
33	3.673765913146663E-4	0.0	0.0	26.90445728833827	0.0
34	3.673765913146663E-4	0.0	0.0	27.469666174075886	0.0
35	3.673765913146663E-4	0.0	0.0	28.055815525518437	0.0
36	3.673765913146663E-4	0.0	0.0	28.566928208184965	0.0
37	4.592207391433329E-4	0.0	0.0	29.101461148547806	0.0
38	4.592207391433329E-4	0.0	0.0	29.67971190327709	0.0
39	4.592207391433329E-4	0.0	0.0	30.424292409724092	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	20	7.0331234E-4	45.0	1
ATTAACG	30	2.165396E-6	44.999996	1
CGTTTTT	3260	0.0	43.136505	1
TACGGGT	140	0.0	41.785717	4
CGTTAGG	155	0.0	40.645164	2
TCGTTAG	50	1.0822987E-9	40.500004	1
CGTAAGG	145	0.0	40.34483	2
GTTTACG	45	1.9281288E-8	40.0	1
TTAGCCG	65	9.094947E-12	38.07692	1
ATAACGG	65	9.094947E-12	38.07692	2
CGTTATT	280	0.0	37.76786	1
TAGCACG	60	1.5643309E-10	37.499996	1
CGCAATA	30	1.1398825E-4	37.499996	15
CGACTAA	30	1.1398825E-4	37.499996	35
GCGTTAG	85	0.0	37.058823	1
ACGGGTC	170	0.0	37.058823	5
GTACGAG	55	2.748493E-9	36.81818	1
TACGCGG	80	0.0	36.5625	2
GCGTAAG	80	0.0	36.5625	1
ACGGGCG	235	0.0	36.38298	5
>>END_MODULE