##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935895.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350885
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77691836356641	31.0	31.0	34.0	30.0	34.0
2	31.820978383230972	33.0	31.0	34.0	30.0	34.0
3	31.86484745714408	33.0	31.0	34.0	30.0	34.0
4	35.6086353078644	37.0	35.0	37.0	33.0	37.0
5	33.59551134987247	37.0	35.0	37.0	32.0	37.0
6	34.53338843210739	37.0	35.0	37.0	30.0	37.0
7	35.57623152884848	37.0	35.0	37.0	32.0	37.0
8	35.50240962138592	37.0	35.0	37.0	33.0	37.0
9	37.370303660743545	39.0	37.0	39.0	34.0	39.0
10	37.03899568234607	39.0	37.0	39.0	33.0	39.0
11	37.05955227496188	39.0	37.0	39.0	33.0	39.0
12	36.95228351169187	39.0	35.0	39.0	33.0	39.0
13	36.75830827764082	39.0	35.0	39.0	33.0	39.0
14	37.796665574190975	40.0	36.0	41.0	33.0	41.0
15	37.86868917166593	40.0	36.0	41.0	33.0	41.0
16	37.96127506162988	40.0	36.0	41.0	33.0	41.0
17	37.89287088362284	40.0	36.0	41.0	33.0	41.0
18	37.881892927882355	39.0	36.0	41.0	33.0	41.0
19	37.839688216937176	39.0	36.0	41.0	33.0	41.0
20	37.783439018481836	40.0	36.0	41.0	33.0	41.0
21	37.77105034412984	39.0	36.0	41.0	33.0	41.0
22	37.845108796329285	39.0	36.0	41.0	33.0	41.0
23	37.81347735012896	39.0	36.0	41.0	34.0	41.0
24	37.76291662510509	39.0	36.0	41.0	33.0	41.0
25	37.621616769026886	39.0	35.0	41.0	33.0	41.0
26	37.635290764780486	39.0	35.0	41.0	33.0	41.0
27	37.648602818587285	39.0	36.0	41.0	33.0	41.0
28	37.51840631545948	39.0	35.0	41.0	33.0	41.0
29	37.50293400971828	39.0	36.0	41.0	33.0	41.0
30	37.438821836214146	39.0	36.0	41.0	33.0	41.0
31	37.405491827806834	39.0	35.0	41.0	33.0	41.0
32	37.26528919731536	39.0	35.0	41.0	33.0	41.0
33	37.21521866138478	39.0	35.0	41.0	33.0	41.0
34	37.12705302307024	39.0	35.0	41.0	32.0	41.0
35	37.114946492440545	39.0	35.0	41.0	32.0	41.0
36	37.0377018111347	39.0	35.0	41.0	32.0	41.0
37	36.95469170810949	39.0	35.0	41.0	32.0	41.0
38	36.83444718355017	39.0	35.0	41.0	31.0	41.0
39	36.81545235618508	39.0	35.0	41.0	31.0	41.0
40	36.71019279821024	39.0	35.0	41.0	31.0	41.0
41	36.66440286703621	39.0	35.0	41.0	31.0	41.0
42	36.69457229576642	39.0	35.0	41.0	31.0	41.0
43	36.60024509454664	39.0	35.0	41.0	31.0	41.0
44	36.47664904455876	39.0	35.0	41.0	31.0	41.0
45	36.447497043190786	39.0	35.0	40.0	31.0	41.0
46	36.36264018125597	39.0	35.0	40.0	31.0	41.0
47	36.265175199851804	39.0	35.0	40.0	30.0	41.0
48	36.1957592943557	38.0	35.0	40.0	30.0	41.0
49	36.155144848027135	38.0	35.0	40.0	30.0	41.0
50	36.004101058751445	38.0	35.0	40.0	30.0	41.0
51	34.71550508001197	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	8.0
12	8.0
13	5.0
14	8.0
15	8.0
16	17.0
17	43.0
18	83.0
19	169.0
20	336.0
21	614.0
22	906.0
23	1242.0
24	1586.0
25	2153.0
26	2700.0
27	3312.0
28	3628.0
29	4383.0
30	5423.0
31	7232.0
32	9689.0
33	14166.0
34	24531.0
35	32176.0
36	27694.0
37	40806.0
38	69119.0
39	98788.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21542670675577	18.680764352993144	22.336948002906933	12.766860937344143
2	29.32955241745871	30.586374453168418	22.64616612280377	17.4379070065691
3	28.81855878706699	30.209042848796614	25.642874446043574	15.329523918092821
4	28.041381079270987	24.007865824985394	29.805206834147942	18.14554626159568
5	26.165267822790945	31.96830870513131	21.99011072003648	19.876312752041265
6	23.314476252903376	37.89019194322926	24.35213816492583	14.443193638941535
7	73.27756957407698	5.841800019949557	15.568918591561337	5.31171181441213
8	71.23843994471123	10.507146215996695	11.089673254770082	7.1647405845219945
9	65.46703335850778	7.483648488821124	13.528933981218918	13.520384171452186
10	38.81442637901307	25.787936218419137	20.021944511734613	15.375692890833179
11	31.955198996822322	23.754506462231216	25.508927426364764	18.781367114581702
12	28.67378200835031	19.630363224418257	30.01866708465737	21.67718768257406
13	23.13350527950753	25.807885774541518	33.024210211322796	18.034398734628155
14	18.280633255910057	31.424825797626006	27.310942331533123	22.983598614930816
15	16.362340937914134	24.520569417330464	39.96665574190974	19.150433902845663
16	18.02499394388475	24.641691722359177	32.52091140972113	24.81240292403494
17	18.163215868446926	24.347863260042462	30.632828419567666	26.856092451942942
18	19.73068099234792	25.226213716744798	31.72492412043832	23.318181170468957
19	21.18956353221141	26.975504795018313	28.09752483007253	23.73740684269775
20	23.347535517334737	25.17320489619106	32.23591775083005	19.243341835644156
21	21.436938028128875	29.594596520227423	29.185060632400926	19.783404819242772
22	18.756287672599285	25.02044829502544	29.079897972270118	27.143366060105162
23	18.69529902959659	29.09671259814469	29.08217792154125	23.12581045071747
24	22.295623922367728	23.257762514784048	29.364891631161207	25.081721931687017
25	17.879362184191404	30.14064437066275	27.62614531826667	24.35384812687918
26	17.632272681932825	27.837325619504966	29.723413654046198	24.80698804451601
27	22.34863274292147	27.653219715861322	26.267010559015063	23.73113698220215
28	17.966285250153184	26.85238753437736	31.853741254257095	23.327585961212364
29	20.00512988586004	27.53950724596378	27.856135200991776	24.599227667184405
30	20.01538965758012	26.233951294583697	29.107542357182552	24.64311669065363
31	24.315088989269988	26.952420308648133	23.004403152029866	25.72808755005201
32	22.793792838109354	30.912977186257606	25.100816506832725	21.192413468800318
33	22.705444803853116	24.905310856833434	26.28525015318409	26.10399418612936
34	21.348305000213745	26.010516266013084	27.513287829345796	25.127890904427375
35	18.116476908388787	25.266112828989556	28.50022087008564	28.11718939253602
36	24.95660971543383	26.57252376134631	26.502700314917995	21.968166208301867
37	20.336007523832595	26.568248856462944	29.153141342605128	23.942602277099333
38	22.34663778730923	25.70072815879847	28.05449078757998	23.898143266312324
39	23.10187098337062	25.706143038317396	28.271085968337207	22.92090000997478
40	21.01742736224119	22.15483705487553	33.930775040255355	22.896960542627927
41	19.903957136953704	24.34643829174801	26.851532553400688	28.898072017897604
42	22.782393091753708	22.781538110777035	30.857688416432733	23.57838038103652
43	26.306054690283144	23.507986947290423	26.02106103139205	24.164897331034386
44	21.53298089117517	23.60915969619676	28.764124998218787	26.09373441440928
45	19.563959701896632	23.769896119811335	27.489633355657837	29.176510822634192
46	24.360117987374778	25.19999430012682	27.62386536899554	22.816022343502855
47	18.775382247744986	22.909500263619133	34.04819242771848	24.266925060917394
48	21.343745101671487	22.31015859897117	29.41020562292489	26.93589067643245
49	21.101785485272952	20.334297561879247	34.574290722031435	23.989626230816363
50	21.66835287914844	20.70649928039101	30.995340353677133	26.62980748678342
51	19.739515795773542	21.523576100431765	27.039058380951026	31.697849722843667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	177.0
1	369.0
2	561.0
3	1262.0
4	1963.0
5	1351.5
6	740.0
7	731.0
8	722.0
9	790.0
10	858.0
11	866.5
12	875.0
13	863.5
14	852.0
15	863.5
16	875.0
17	851.0
18	827.0
19	710.0
20	593.0
21	802.0
22	1011.0
23	1108.5
24	1206.0
25	1463.5
26	2048.5
27	2376.0
28	2884.5
29	3393.0
30	3503.5
31	3614.0
32	4480.0
33	5346.0
34	6467.0
35	7588.0
36	7839.0
37	8090.0
38	9266.0
39	10442.0
40	12635.5
41	14829.0
42	17099.5
43	19370.0
44	23057.0
45	26744.0
46	39787.5
47	52831.0
48	47304.5
49	41778.0
50	41331.0
51	40884.0
52	33186.0
53	25488.0
54	21740.5
55	17993.0
56	16074.5
57	14156.0
58	12483.5
59	10811.0
60	10453.0
61	10095.0
62	8811.5
63	7528.0
64	6138.0
65	4748.0
66	4186.5
67	3625.0
68	3057.5
69	2490.0
70	2010.5
71	1531.0
72	1272.0
73	1013.0
74	780.0
75	415.5
76	284.0
77	200.5
78	117.0
79	95.0
80	73.0
81	87.5
82	102.0
83	59.5
84	17.0
85	9.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.033976877013345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.28940866099965	20.209685437923657
2	13.851355943786272	8.320226082619167
3	5.314598921174686	4.788556233274818
4	2.636893803796507	3.1678563012145617
5	1.4899712750442733	2.23748814110469
6	0.9971364106751873	1.7968783140867992
7	0.7105138185684274	1.4937688918377823
8	0.596072202964746	1.432193500869887
9	0.4955654026085841	1.3395419858694624
>10	6.409510241558645	41.29775404109268
>50	0.14528621213407203	2.8500378635912447
>100	0.050750663142723786	2.7869759939165646
>500	0.005970666252085153	1.5170713292354403
>1k	0.005970666252085153	4.953793790795395
>5k	9.95111042014192E-4	1.8081720925678515
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	6050	1.7242116362910926	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	4882	1.3913390427062997	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	4852	1.3827892329395672	No Hit
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2908	0.8287615600552887	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	1799	0.5127035923450703	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCT	1127	0.32118785357025803	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1007	0.2869886145033273	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGATCTGT	940	0.2678940393576243	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	935	0.26646907106316886	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT	915	0.2607691978853471	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTC	841	0.23967966712740643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGATCTGTCGTA	830	0.23654473687960442	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	615	0.17527110021802014	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	387	0.11029254599085171	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	369	0.10516266013081209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.699873177821794E-4	0.0	0.0	0.28527865254998075	0.0
2	5.699873177821794E-4	0.0	0.0	1.9909657010131525	0.0
3	5.699873177821794E-4	0.0	0.0	2.523048862162817	0.0
4	5.699873177821794E-4	0.0	0.0	3.4153640081508185	0.0
5	5.699873177821794E-4	0.0	0.0	6.597033216010944	0.0
6	5.699873177821794E-4	0.0	0.0	7.804551348732491	0.0
7	5.699873177821794E-4	0.0	0.0	9.248899211992534	0.0
8	5.699873177821794E-4	0.0	0.0	11.318238169200736	0.0
9	5.699873177821794E-4	0.0	0.0	12.019037576413925	0.0
10	5.699873177821794E-4	0.0	0.0	14.912293201476267	0.0
11	5.699873177821794E-4	0.0	0.0	16.852530031206804	0.0
12	5.699873177821794E-4	0.0	0.0	20.228849908089543	0.0
13	5.699873177821794E-4	0.0	0.0	21.005172634908874	0.0
14	5.699873177821794E-4	0.0	0.0	21.338615215811448	0.0
15	5.699873177821794E-4	0.0	0.0	22.107528107499608	0.0
16	5.699873177821794E-4	0.0	0.0	23.220428345469312	0.0
17	8.54980976673269E-4	0.0	0.0	24.376647619590464	0.0
18	8.54980976673269E-4	0.0	0.0	25.75060204910441	0.0
19	8.54980976673269E-4	0.0	0.0	26.75320974108326	0.0
20	8.54980976673269E-4	0.0	0.0	27.509012924462432	0.0
21	8.54980976673269E-4	0.0	0.0	28.390783305071462	0.0
22	8.54980976673269E-4	0.0	0.0	29.301338045228494	0.0
23	8.54980976673269E-4	0.0	0.0	30.158598971172893	0.0
24	8.54980976673269E-4	0.0	0.0	30.833178961768102	0.0
25	0.0011399746355643587	0.0	0.0	31.4632999415763	0.0
26	0.0011399746355643587	0.0	0.0	32.0327172720407	0.0
27	0.0011399746355643587	0.0	0.0	32.64231870840874	0.0
28	0.0011399746355643587	0.0	0.0	33.17810678712399	0.0
29	0.0011399746355643587	0.0	0.0	33.733844421961614	0.0
30	0.0011399746355643587	0.0	0.0	34.41298431109908	0.0
31	0.0011399746355643587	0.0	0.0	35.02771563332716	0.0
32	0.0011399746355643587	0.0	0.0	35.58744317938926	0.0
33	0.0011399746355643587	0.0	0.0	36.1523006113114	0.0
34	0.0011399746355643587	0.0	0.0	36.640494748991834	0.0
35	0.0011399746355643587	0.0	0.0	37.208202117502886	0.0
36	0.0011399746355643587	0.0	0.0	37.778759422602846	0.0
37	0.0011399746355643587	0.0	0.0	38.31882240620146	0.0
38	0.0011399746355643587	0.0	0.0	38.855750459552276	0.0
39	0.0011399746355643587	0.0	0.0	39.38555367143081	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	30	2.1611759E-6	45.000004	4
GCCCTCG	70	0.0	45.000004	13
AGTTATG	30	2.1611759E-6	45.000004	1
GCTACGA	60	0.0	45.000004	10
TGATATG	25	3.8852482E-5	45.0	1
CCTTATC	20	7.02658E-4	45.0	43
CGGCGAA	20	7.02658E-4	45.0	31
TCGTTGA	25	3.8852482E-5	45.0	40
ATAGGCG	20	7.02658E-4	45.0	12
CATGCGG	20	7.02658E-4	45.0	2
CGTGAGT	20	7.02658E-4	45.0	32
CGCATGG	20	7.02658E-4	45.0	2
GGACTAG	20	7.02658E-4	45.0	1
TGAACTA	20	7.02658E-4	45.0	29
TAAGTCC	25	3.8852482E-5	45.0	23
CGCGAGG	40	6.7921064E-9	45.0	2
ACGGGCC	25	3.8852482E-5	45.0	5
ATCGTGA	20	7.02658E-4	45.0	30
GTAATCG	20	7.02658E-4	45.0	15
TGTTGCG	20	7.02658E-4	45.0	1
>>END_MODULE