##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935894.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	850533
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.023385336018706	33.0	31.0	34.0	30.0	34.0
2	32.061890602716176	33.0	31.0	34.0	30.0	34.0
3	32.07610051579422	33.0	31.0	34.0	30.0	34.0
4	35.76062892327517	37.0	35.0	37.0	33.0	37.0
5	33.688722248284314	37.0	35.0	37.0	32.0	37.0
6	34.65997674399465	37.0	35.0	37.0	30.0	37.0
7	35.76923646701539	37.0	35.0	37.0	33.0	37.0
8	35.806350841178414	37.0	35.0	37.0	35.0	37.0
9	37.73385394805375	39.0	38.0	39.0	35.0	39.0
10	37.24264549406078	39.0	37.0	39.0	34.0	39.0
11	37.183721266546975	39.0	37.0	39.0	34.0	39.0
12	37.2767923172881	39.0	37.0	39.0	34.0	39.0
13	37.30524388824419	39.0	37.0	39.0	34.0	39.0
14	38.47196992944424	40.0	38.0	41.0	34.0	41.0
15	38.542221171900444	40.0	38.0	41.0	34.0	41.0
16	38.508429420140075	40.0	38.0	41.0	34.0	41.0
17	38.46286622623696	40.0	38.0	41.0	34.0	41.0
18	38.421579174470594	40.0	38.0	41.0	34.0	41.0
19	38.31264395385011	40.0	37.0	41.0	34.0	41.0
20	38.336722972536045	40.0	37.0	41.0	34.0	41.0
21	38.25240878366859	40.0	37.0	41.0	34.0	41.0
22	38.32277289652488	40.0	37.0	41.0	34.0	41.0
23	38.31101086024881	40.0	37.0	41.0	34.0	41.0
24	38.27877930662302	40.0	37.0	41.0	34.0	41.0
25	38.10069920861389	40.0	37.0	41.0	34.0	41.0
26	38.181861256412155	40.0	37.0	41.0	34.0	41.0
27	38.18545547321503	40.0	37.0	41.0	34.0	41.0
28	38.1009190707474	40.0	37.0	41.0	34.0	41.0
29	38.10084735101401	40.0	37.0	41.0	34.0	41.0
30	37.94714961089105	40.0	37.0	41.0	34.0	41.0
31	37.912491343663326	40.0	37.0	41.0	33.0	41.0
32	37.7989578299725	40.0	37.0	41.0	33.0	41.0
33	37.685262065081545	40.0	37.0	41.0	33.0	41.0
34	37.55740929511259	40.0	37.0	41.0	33.0	41.0
35	37.42589058860738	40.0	37.0	41.0	33.0	41.0
36	37.330987745331456	40.0	36.0	41.0	33.0	41.0
37	37.315991266652794	40.0	36.0	41.0	32.0	41.0
38	37.21298409350372	40.0	36.0	41.0	32.0	41.0
39	37.16436046573149	40.0	36.0	41.0	32.0	41.0
40	37.07729623659517	40.0	36.0	41.0	31.0	41.0
41	36.97402569917922	40.0	36.0	41.0	31.0	41.0
42	36.97655352584791	40.0	36.0	41.0	31.0	41.0
43	36.943263812221275	40.0	36.0	41.0	31.0	41.0
44	36.84310661667448	40.0	35.0	41.0	31.0	41.0
45	36.77672823982138	39.0	35.0	41.0	31.0	41.0
46	36.68643309548248	39.0	35.0	41.0	31.0	41.0
47	36.61100745062214	39.0	35.0	41.0	31.0	41.0
48	36.5474614153713	39.0	35.0	41.0	31.0	41.0
49	36.46707887877366	39.0	35.0	41.0	30.0	41.0
50	36.37315894856519	39.0	35.0	41.0	30.0	41.0
51	35.222647445778115	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	15.0
11	10.0
12	7.0
13	12.0
14	14.0
15	30.0
16	46.0
17	78.0
18	198.0
19	349.0
20	653.0
21	1135.0
22	1725.0
23	2437.0
24	3559.0
25	5288.0
26	7361.0
27	8709.0
28	9341.0
29	9933.0
30	11581.0
31	14242.0
32	18868.0
33	26049.0
34	42964.0
35	56424.0
36	60947.0
37	94841.0
38	172465.0
39	301046.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14788491451831	18.545664894836534	23.968734899174986	14.337715291470172
2	31.524585171886333	25.926095754074208	25.44557354035646	17.103745533682996
3	31.324240211726057	25.71234743390321	27.505693488671223	15.457718865699507
4	26.274700687686426	25.20842812683341	29.57004607698937	18.946825108490795
5	24.671941006404218	33.675001440273334	24.191653939353323	17.46140361396912
6	24.525679779620543	34.95466960129707	24.982687326652815	15.53696329242957
7	81.45586355849802	4.324464776792905	10.298836141572403	3.9208355231366685
8	82.90718878632575	4.91256659059672	8.402025553388286	3.778219069689242
9	77.15996910172798	6.546953498570895	9.896265047917012	6.396812351784116
10	33.14650930651721	38.44718547075775	16.229235079650053	12.177070143074989
11	24.444201459555362	26.04637327417043	32.11727234569382	17.39215292058039
12	22.685892258148712	24.00635836587175	33.059622613114364	20.24812676286517
13	22.066045644319505	25.40947852699425	35.14549112144973	17.378984707236523
14	19.402069055521658	29.152895889988983	31.534461331894235	19.910573722595124
15	18.049623001106365	27.87769551563549	35.25624520153833	18.816436281719813
16	22.080389590997644	28.431230769411652	30.51992103774927	18.968458601841434
17	21.438321617150656	27.701688235494682	30.39611631765023	20.463873829704433
18	23.237546338590036	24.906970099925577	31.340465331739036	20.515018229745348
19	20.822590069991406	28.23817535592387	29.876089463900872	21.06314511018385
20	23.136198125175625	27.688990315484524	30.317342184253874	18.857469375085977
21	22.19796292442504	28.694477462955582	30.97104991811017	18.136509694509208
22	22.391135911246245	24.567888606320977	31.314951918385297	21.726023564047487
23	19.759139269140643	28.614997889558662	30.952473331428642	20.673389509872045
24	20.06365420271759	26.290808234365983	32.30797629251305	21.337561270403384
25	19.154459615323567	29.866918743893535	30.101124824080898	20.877496816702
26	20.167824176134257	27.76941047554886	29.911126317262234	22.151639031054646
27	21.041981910166918	27.407167035259068	29.819301543855442	21.731549510718573
28	18.449137188092642	27.899211435652703	31.131890238238846	22.51976113801581
29	19.693886069088443	26.1097453008878	30.53896791776451	23.657400712259253
30	20.81388964331778	27.905913115658066	29.956039330631494	21.32415791039266
31	23.291630071966637	27.239860181791887	27.413280848597292	22.055228897644184
32	21.50627900387169	27.787281622229827	28.48731324945652	22.219126124441967
33	20.38145492297183	28.544924182835942	27.914025675664554	23.159595218527677
34	21.52485559055322	27.430681701944547	29.298216530105243	21.746246177396998
35	19.459915135567933	28.48096428945144	29.692322343753858	22.366798231226774
36	20.83775703000354	30.67041490453633	27.40199380858826	21.089834256871868
37	20.240484496192387	29.209213516700704	28.925273916473554	21.625028070633356
38	20.778382496622704	29.354181436816678	28.31001266264801	21.55742340391261
39	21.680992977344797	27.771879515550836	27.97986674238389	22.567260764720476
40	22.41194639126289	27.07608052832753	30.321574824257258	20.19039825615232
41	20.41355244299751	27.956822369032125	28.778307249689313	22.851317938281053
42	21.669353217335484	26.88102636817149	29.945340157289607	21.504280257203423
43	21.527677350555475	27.55707303537899	28.507653436139456	22.407596177926077
44	21.460072683834724	26.896898768184187	28.351163329347596	23.291865218633493
45	21.14380041691504	26.35888319442044	28.09532375580959	24.401992632854927
46	22.49048537799239	27.36025527522154	27.39305823524778	22.756201111538292
47	19.855902122551388	27.26913594181531	30.810562317981784	22.06439961765152
48	20.516899403080185	26.95803690156643	29.548295010305303	22.976768685048082
49	21.911436710862482	25.257691353539485	30.801979464641583	22.028892470956446
50	19.835797082535304	26.62177716796409	30.452081224361667	23.090344525138942
51	20.21508865617207	24.860175913221475	28.973361409845356	25.951374020761097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	447.5
2	738.0
3	4076.0
4	7414.0
5	5071.0
6	2728.0
7	2695.5
8	2663.0
9	2648.5
10	2634.0
11	2670.0
12	2706.0
13	2642.5
14	2579.0
15	2473.5
16	2368.0
17	2462.5
18	2557.0
19	2601.0
20	2645.0
21	2932.0
22	3219.0
23	4046.0
24	4873.0
25	5752.0
26	7903.5
27	9176.0
28	11109.0
29	13042.0
30	15640.0
31	18238.0
32	21055.5
33	23873.0
34	27317.5
35	30762.0
36	33223.5
37	35685.0
38	37827.5
39	39970.0
40	43168.0
41	46366.0
42	50011.5
43	53657.0
44	57478.5
45	61300.0
46	75304.5
47	89309.0
48	84808.5
49	80308.0
50	79589.5
51	78871.0
52	70492.5
53	62114.0
54	54560.0
55	47006.0
56	40346.5
57	33687.0
58	29674.5
59	25662.0
60	22322.0
61	18982.0
62	16459.5
63	13937.0
64	11711.0
65	9485.0
66	7663.5
67	5842.0
68	4730.0
69	3618.0
70	2823.0
71	2028.0
72	1717.0
73	1406.0
74	1229.5
75	869.0
76	685.0
77	519.5
78	354.0
79	240.5
80	127.0
81	77.0
82	27.0
83	31.5
84	36.0
85	22.5
86	9.0
87	5.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	850533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.41804078026092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86983512939727	23.622972868756904
2	9.674725226786236	6.272712738795505
3	3.654045232597569	3.553709620897979
4	2.0062632861219925	2.601565001017721
5	1.2525730095632535	2.0302981452137847
6	1.0091743475224677	1.9629273091425938
7	0.7986297792804239	1.8123008927130486
8	0.6626700189154203	1.7185970957645094
9	0.6093806641695123	1.7779434499574758
>10	7.319935524724026	47.46167194289937
>50	0.10762494670658387	2.186196278234111
>100	0.029651766369075756	1.646945386929197
>500	0.0021964271384499793	0.49771501317055106
>1k	0.0029285695179333054	2.117246912249833
>5k	3.660711897416632E-4	0.7371973442573827
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	6212	0.7303655472509591	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3934	0.4625334937033601	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	3426	0.402806240322245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	3133	0.36835725362801913	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	2079	0.24443496019554795	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	1921	0.22585837351402002	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	1176	0.13826624011061298	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1163	0.1367377867760569	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTG	1009	0.11863149342823853	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	854	0.10040762674699277	TruSeq Adapter, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.35146666854784E-4	0.0	0.0	0.10828504008662804	0.0
2	2.35146666854784E-4	0.0	0.0	0.5939804804751844	0.0
3	2.35146666854784E-4	0.0	0.0	0.8814472807051579	0.0
4	2.35146666854784E-4	0.0	0.0	1.3953603211162882	0.0
5	2.35146666854784E-4	0.0	0.0	2.8455098156097414	0.0
6	2.35146666854784E-4	0.0	0.0	3.7850383230280307	0.0
7	2.35146666854784E-4	0.0	0.0	4.550911016974062	0.0
8	2.35146666854784E-4	0.0	0.0	5.750159017933461	0.0
9	2.35146666854784E-4	0.0	0.0	6.249257818332739	0.0
10	2.35146666854784E-4	0.0	0.0	7.531395019358449	0.0
11	2.35146666854784E-4	0.0	0.0	8.504784646803827	0.0
12	2.35146666854784E-4	0.0	0.0	9.815727314519249	0.0
13	2.35146666854784E-4	0.0	0.0	10.270383394882973	0.0
14	2.35146666854784E-4	0.0	0.0	10.493067288394453	0.0
15	2.35146666854784E-4	0.0	0.0	10.802873021975632	0.0
16	2.35146666854784E-4	0.0	0.0	11.301854249041483	0.0
17	3.52720000282176E-4	0.0	0.0	11.96108792956887	0.0
18	3.52720000282176E-4	0.0	0.0	12.617147130093718	0.0
19	3.52720000282176E-4	0.0	0.0	13.133176490506541	0.0
20	3.52720000282176E-4	0.0	0.0	13.56467062418507	0.0
21	3.52720000282176E-4	0.0	0.0	14.06694390458689	0.0
22	3.52720000282176E-4	0.0	0.0	14.646345291717076	0.0
23	3.52720000282176E-4	0.0	0.0	15.200938705494083	0.0
24	3.52720000282176E-4	0.0	0.0	15.643014439181078	0.0
25	3.52720000282176E-4	0.0	0.0	16.039706866165098	0.0
26	3.52720000282176E-4	0.0	0.0	16.410885879795376	0.0
27	3.52720000282176E-4	0.0	0.0	16.82403857345923	0.0
28	3.52720000282176E-4	0.0	0.0	17.214264467104744	0.0
29	3.52720000282176E-4	0.0	0.0	17.63012134743743	0.0
30	3.52720000282176E-4	0.0	0.0	18.178013081209077	0.0
31	3.52720000282176E-4	0.0	0.0	18.637607241576752	0.0
32	4.70293333709568E-4	0.0	0.0	19.10072860194725	0.0
33	4.70293333709568E-4	0.0	0.0	19.541746175633396	0.0
34	5.8786666713696E-4	0.0	0.0	19.97030097597624	0.0
35	5.8786666713696E-4	0.0	0.0	20.434833216347865	0.0
36	5.8786666713696E-4	0.0	0.0	20.846927750010874	0.0
37	5.8786666713696E-4	0.0	0.0	21.26689969701352	0.0
38	5.8786666713696E-4	0.0	0.0	21.712972924037047	0.0
39	5.8786666713696E-4	0.0	0.0	22.199373804426166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.03225E-4	45.0	1
TATTGCG	55	1.8189894E-12	45.0	1
TTACGCG	20	7.03225E-4	45.0	1
AGTACGG	60	0.0	44.999996	2
CGCACGG	95	0.0	42.63158	2
CGTTTTT	2685	0.0	42.486034	1
TTGTACG	55	6.002665E-11	40.909092	1
GCACGCG	50	1.0804797E-9	40.500004	1
TACGCGG	95	0.0	40.263157	2
TCGCGCG	45	1.9272193E-8	40.000004	1
GACGGGT	85	0.0	39.705883	4
TTTCGCG	40	3.4568075E-7	39.375	1
AGGCGAT	40	3.4568075E-7	39.375	6
CTTACGG	80	0.0	39.375	2
CGAAAGG	180	0.0	38.750004	2
ACGGGAA	630	0.0	38.57143	5
TGATCGA	35	6.2463732E-6	38.571426	21
CGGCGAA	35	6.2463732E-6	38.571426	31
CGACAGG	70	0.0	38.571426	2
AATAGCG	35	6.2463732E-6	38.571426	1
>>END_MODULE