##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935893.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339989
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80464662092009	33.0	31.0	34.0	30.0	34.0
2	31.866339793346256	33.0	31.0	34.0	30.0	34.0
3	31.910917706161083	33.0	31.0	34.0	30.0	34.0
4	35.61125507001697	37.0	35.0	37.0	33.0	37.0
5	33.539746874163576	37.0	35.0	37.0	32.0	37.0
6	34.527775898632015	37.0	35.0	37.0	30.0	37.0
7	35.64898570247861	37.0	35.0	37.0	32.0	37.0
8	35.63957069199298	37.0	35.0	37.0	33.0	37.0
9	37.507648776872195	39.0	37.0	39.0	35.0	39.0
10	37.07950257214204	39.0	37.0	39.0	33.0	39.0
11	37.121053916450236	39.0	37.0	39.0	34.0	39.0
12	36.99043204338964	39.0	35.0	39.0	33.0	39.0
13	36.84702446255614	39.0	35.0	39.0	33.0	39.0
14	37.860927853548205	40.0	37.0	41.0	33.0	41.0
15	37.99081440870146	40.0	37.0	41.0	33.0	41.0
16	38.03204809567368	40.0	36.0	41.0	33.0	41.0
17	38.018371182597086	40.0	36.0	41.0	33.0	41.0
18	38.00928265326231	40.0	36.0	41.0	34.0	41.0
19	37.92095038368888	40.0	36.0	41.0	34.0	41.0
20	37.95619270035207	40.0	36.0	41.0	34.0	41.0
21	37.89279359038087	40.0	36.0	41.0	34.0	41.0
22	37.95721626287909	40.0	36.0	41.0	34.0	41.0
23	37.957678042524904	40.0	36.0	41.0	34.0	41.0
24	37.88922876916606	40.0	36.0	41.0	34.0	41.0
25	37.701034445232054	39.0	36.0	41.0	33.0	41.0
26	37.76805131930739	40.0	36.0	41.0	33.0	41.0
27	37.75215962869387	40.0	36.0	41.0	33.0	41.0
28	37.65587416063461	40.0	36.0	41.0	33.0	41.0
29	37.680895558385714	40.0	36.0	41.0	33.0	41.0
30	37.55195903396874	40.0	36.0	41.0	33.0	41.0
31	37.46291203538938	39.0	35.0	41.0	33.0	41.0
32	37.40818085290995	39.0	35.0	41.0	33.0	41.0
33	37.33583145337055	39.0	35.0	41.0	33.0	41.0
34	37.23657235969399	39.0	35.0	41.0	33.0	41.0
35	37.2261102565083	40.0	35.0	41.0	33.0	41.0
36	37.09694431290424	39.0	35.0	41.0	32.0	41.0
37	37.015494618943556	39.0	35.0	41.0	32.0	41.0
38	36.919688578159885	39.0	35.0	41.0	31.0	41.0
39	36.92763589410246	39.0	35.0	41.0	32.0	41.0
40	36.85235992929183	39.0	35.0	41.0	31.0	41.0
41	36.756692128274736	39.0	35.0	41.0	31.0	41.0
42	36.75827453241134	39.0	35.0	41.0	31.0	41.0
43	36.68313092482404	39.0	35.0	41.0	31.0	41.0
44	36.52661997888167	39.0	35.0	41.0	31.0	41.0
45	36.486468679869056	39.0	35.0	41.0	31.0	41.0
46	36.421431281600285	39.0	35.0	40.0	31.0	41.0
47	36.323710472985894	39.0	35.0	40.0	31.0	41.0
48	36.2748912464815	38.0	35.0	40.0	31.0	41.0
49	36.17981758233355	38.0	35.0	40.0	30.0	41.0
50	36.03568938995085	38.0	35.0	40.0	30.0	41.0
51	34.78510187094288	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	8.0
11	3.0
12	5.0
13	7.0
14	7.0
15	8.0
16	21.0
17	27.0
18	60.0
19	108.0
20	309.0
21	436.0
22	673.0
23	944.0
24	1330.0
25	1924.0
26	2487.0
27	3134.0
28	3514.0
29	4260.0
30	5339.0
31	6856.0
32	9359.0
33	13098.0
34	22878.0
35	31462.0
36	26433.0
37	39778.0
38	67848.0
39	97619.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.537846812691	19.847112700705022	24.268726341146333	13.346314145457647
2	29.83067099229681	26.034077573097953	25.987899608516745	18.14735182608849
3	30.680404366023605	25.802305368703106	26.82939742168129	16.687892843591996
4	26.857045374997423	24.83227398533482	29.828906229319184	18.48177441034857
5	24.884922747500653	32.337516802014186	24.412848650985765	18.364711799499396
6	23.836947665953897	34.837009432658114	25.29405363114689	16.031989270241095
7	77.11220068884582	5.077811340955148	12.5227580892323	5.287229880966738
8	76.48600395895161	6.491974740359247	10.039442452549935	6.982578848139205
9	70.6955225021986	6.260790790290274	12.952772001447105	10.09091470606402
10	38.44036130580695	25.904955748568337	20.155063840300716	15.499619105323994
11	31.064240313657205	22.85250405160167	25.96436943548174	20.118886199259386
12	27.352943771710258	20.294774242696086	30.603931303659827	21.74835068193383
13	22.013359255740628	25.885249228651514	33.5478500775025	18.553541438105352
14	18.698840256596537	30.127445299700874	28.61769057234205	22.556023871360544
15	15.782275308907053	25.399057028315625	38.90890587636659	19.909761786410737
16	18.713546614743418	24.429319772110276	33.19636811779205	23.660765495354262
17	18.74148869522249	23.91842089008762	29.808611455076488	27.5314789596134
18	20.496545476471297	23.459582515904927	33.17930874234166	22.86456326528211
19	20.593607440240714	26.380559371038476	28.666809808552628	24.359023380168182
20	22.53278782548847	26.156728600042946	30.21185979546397	21.098623779004615
21	21.420398895258376	27.995611622728973	29.481836177052788	21.102153304959867
22	18.836491768851346	24.929630076267173	29.510072384694798	26.723805770186683
23	18.895023074275933	27.215880513781325	28.660339010968	25.22875740097474
24	20.7639070675816	23.4234048748636	31.318072055272378	24.494616002282427
25	17.770280803202457	27.931491901208567	29.515660800790616	24.78256649479836
26	18.440008353211425	25.64288844639091	29.578604013659266	26.338499186738396
27	21.521872766471855	27.33647265058575	27.935021427163818	23.20663315577857
28	16.847015638741254	26.84410377982817	32.5557591569139	23.753121424516678
29	19.0526752336105	22.757500977972818	30.993649794552177	27.19617399386451
30	21.766586566035958	26.493798328769458	29.934497880813794	21.805117224380787
31	22.076596595772216	27.953845565591827	25.74436231760439	24.225195521031562
32	22.949860142534025	25.61612287456359	27.378826962048773	24.055190020853615
33	23.763415875219494	24.773154425584355	27.29470659344861	24.16872310574754
34	19.051498724958748	26.55703566880105	28.555041486636334	25.83642411960387
35	20.026530270096973	23.27075287729897	28.419154737359147	28.283562115244905
36	24.300786201906533	25.482000888264032	27.455005897249617	22.762207012579818
37	20.86743982893564	27.587363120571545	30.41539579221681	21.129801258276004
38	18.68325151696084	27.603540114533114	29.020938912729527	24.69226945577651
39	21.34598472303516	25.424940218654136	31.4589589663195	21.770116091991213
40	21.04979866995697	23.176926312321868	30.865998605837248	24.907276411883913
41	20.080061413751622	25.28287679895526	26.756748012435693	27.880313774857424
42	22.507492889475834	22.58867198644662	27.893549497189614	27.01028562688793
43	24.528146498857318	22.428078555482678	28.918582660027237	24.12519228563277
44	20.256831838677133	24.4904982220013	30.044501439752462	25.208168499569105
45	19.33827270882293	24.285785716596713	28.500333834329933	27.87560774025042
46	23.623117218498248	24.370788466685685	28.696810779172267	23.3092835356438
47	18.670604048954527	24.15166373029716	32.44310845350879	24.734623767239526
48	21.03950421925415	22.981625876131286	29.0715287847548	26.907341119859762
49	21.418340005117813	20.899205562532906	32.95312495404263	24.72932947830665
50	20.619784757742163	21.26245260876087	31.632199865289763	26.485562768207206
51	18.922082773266194	22.050713405433704	28.83740356305645	30.18980025824365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	281.0
2	410.0
3	1134.0
4	1858.0
5	1254.5
6	651.0
7	680.5
8	710.0
9	715.5
10	721.0
11	738.5
12	756.0
13	751.5
14	747.0
15	840.0
16	933.0
17	817.0
18	701.0
19	741.5
20	782.0
21	807.5
22	833.0
23	996.5
24	1160.0
25	1394.0
26	1765.5
27	1903.0
28	2151.0
29	2399.0
30	3150.5
31	3902.0
32	4766.5
33	5631.0
34	6627.0
35	7623.0
36	7976.5
37	8330.0
38	10081.5
39	11833.0
40	13314.5
41	14796.0
42	17684.0
43	20572.0
44	23583.5
45	26595.0
46	34380.5
47	42166.0
48	42321.0
49	42476.0
50	41730.0
51	40984.0
52	33231.5
53	25479.0
54	21913.5
55	18348.0
56	16395.5
57	14443.0
58	12604.5
59	10766.0
60	9675.5
61	8585.0
62	7989.0
63	7393.0
64	6177.5
65	4962.0
66	4273.5
67	3585.0
68	2775.0
69	1965.0
70	1670.0
71	1375.0
72	1141.5
73	908.0
74	681.0
75	352.5
76	251.0
77	180.5
78	110.0
79	79.0
80	48.0
81	50.5
82	53.0
83	28.0
84	3.0
85	2.5
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.887790725170984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.88256172370433	20.658545696851103
2	14.151985346568086	8.742471234609628
3	5.465903502073705	5.064890504880953
4	2.6690168405700447	3.297601344539384
5	1.555965962702735	2.403017551572564
6	0.9236979954756893	1.7118594226507813
7	0.6967383495002298	1.5064495830694895
8	0.552411671230121	1.3650220876078347
9	0.46482173305549945	1.2921584773616603
>10	6.441811124511737	44.45602457765045
>50	0.1453189774936132	2.887142131066265
>100	0.0408087539536859	2.3060859466646844
>500	0.0029860063868550656	0.6327056230150541
>1k	0.005972012773710131	3.676025818460156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	2671	0.7856136522063949	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	2298	0.675904220430661	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	2263	0.6656097697278441	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	2239	0.6585507178173412	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1464	0.4306021665406822	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.3005979605222522	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	896	0.2635379379921115	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	646	0.1900061472577054	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	516	0.15176961607581424	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT	445	0.13088658750724289	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	387	0.11382721205686067	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	386	0.11353308489392305	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA	386	0.11353308489392305	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC	376	0.1105918132645468	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.941271629376245E-4	0.0	0.0	0.28412683939774525	0.0
2	2.941271629376245E-4	0.0	0.0	1.7709396480474369	0.0
3	2.941271629376245E-4	0.0	0.0	2.3177220439484807	0.0
4	2.941271629376245E-4	0.0	0.0	3.1227480889087587	0.0
5	2.941271629376245E-4	0.0	0.0	5.485765715949634	0.0
6	2.941271629376245E-4	0.0	0.0	6.650509281182626	0.0
7	2.941271629376245E-4	0.0	0.0	7.985258346593566	0.0
8	2.941271629376245E-4	0.0	0.0	10.162387606657862	0.0
9	2.941271629376245E-4	0.0	0.0	10.913294253637618	0.0
10	2.941271629376245E-4	0.0	0.0	13.186014841656641	0.0
11	2.941271629376245E-4	0.0	0.0	15.121959828112086	0.0
12	2.941271629376245E-4	0.0	0.0	17.53203780122298	0.0
13	2.941271629376245E-4	0.0	0.0	18.242354899717345	0.0
14	2.941271629376245E-4	0.0	0.0	18.51383427110877	0.0
15	2.941271629376245E-4	0.0	0.0	19.103853359961647	0.0
16	2.941271629376245E-4	0.0	0.0	20.13594557470977	0.0
17	2.941271629376245E-4	0.0	0.0	21.39745697654924	0.0
18	2.941271629376245E-4	0.0	0.0	22.819267682189718	0.0
19	2.941271629376245E-4	0.0	0.0	23.70811996858722	0.0
20	2.941271629376245E-4	0.0	0.0	24.495204256608304	0.0
21	2.941271629376245E-4	0.0	0.0	25.35729097117848	0.0
22	2.941271629376245E-4	0.0	0.0	26.227907373473847	0.0
23	2.941271629376245E-4	0.0	0.0	27.186761924650504	0.0
24	2.941271629376245E-4	0.0	0.0	27.927962375253315	0.0
25	2.941271629376245E-4	0.0	0.0	28.584454202930093	0.0
26	2.941271629376245E-4	0.0	0.0	29.173885037457094	0.0
27	2.941271629376245E-4	0.0	0.0	29.79861113153661	0.0
28	2.941271629376245E-4	0.0	0.0	30.40510134151399	0.0
29	2.941271629376245E-4	0.0	0.0	31.021297747868314	0.0
30	2.941271629376245E-4	0.0	0.0	31.774851539314508	0.0
31	2.941271629376245E-4	0.0	0.0	32.40604843097865	0.0
32	2.941271629376245E-4	0.0	0.0	33.01400927677072	0.0
33	2.941271629376245E-4	0.0	0.0	33.606675510090035	0.0
34	2.941271629376245E-4	0.0	0.0	34.18434125809953	0.0
35	2.941271629376245E-4	0.0	0.0	34.804949571897914	0.0
36	2.941271629376245E-4	0.0	0.0	35.377615158137466	0.0
37	2.941271629376245E-4	0.0	0.0	35.90645579709932	0.0
38	2.941271629376245E-4	0.0	0.0	36.45264993867448	0.0
39	2.941271629376245E-4	0.0	0.0	37.01972710881822	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	60	0.0	45.000004	6
ATCTACC	25	3.8849903E-5	45.000004	15
GGGTCGT	25	3.8849903E-5	45.000004	7
GTTAGCG	30	2.1609758E-6	45.000004	1
ATGTCCG	25	3.8849903E-5	45.000004	11
GTTACGG	30	2.1609758E-6	45.000004	2
CGGATGG	30	2.1609758E-6	45.000004	2
TACGAAT	50	2.1827873E-11	45.000004	12
ATGCAGC	30	2.1609758E-6	45.000004	42
GTACGAG	25	3.8849903E-5	45.000004	1
GCATATC	50	2.1827873E-11	45.000004	28
TCACGAC	20	7.0262706E-4	45.0	25
CGACGGT	20	7.0262706E-4	45.0	28
TTCCGTA	20	7.0262706E-4	45.0	18
ATATGCG	20	7.0262706E-4	45.0	1
TCCTAAG	20	7.0262706E-4	45.0	20
AATGCGG	40	6.7902874E-9	45.0	2
CCGTACA	20	7.0262706E-4	45.0	20
TACGAAA	20	7.0262706E-4	45.0	20
CGAGGGT	45	3.8380676E-10	45.0	4
>>END_MODULE