##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935892.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174040
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955224694218256	33.0	31.0	34.0	30.0	34.0
2	32.01822254778372	33.0	31.0	34.0	30.0	34.0
3	31.958808047425983	33.0	31.0	34.0	30.0	34.0
4	35.70409355728936	37.0	35.0	37.0	33.0	37.0
5	33.629105481925656	37.0	35.0	37.0	32.0	37.0
6	34.623243671425165	37.0	35.0	37.0	30.0	37.0
7	35.72868897141495	37.0	35.0	37.0	32.0	37.0
8	35.78678835474089	37.0	35.0	37.0	35.0	37.0
9	37.664664747368064	39.0	37.0	39.0	35.0	39.0
10	37.24724796429423	39.0	37.0	39.0	34.0	39.0
11	37.246589554018605	39.0	37.0	39.0	34.0	39.0
12	37.24125498279445	39.0	37.0	39.0	34.0	39.0
13	37.24787741473885	39.0	37.0	39.0	34.0	39.0
14	38.30828677046779	40.0	38.0	41.0	33.0	41.0
15	38.42719754011788	40.0	38.0	41.0	34.0	41.0
16	38.395943920139004	40.0	38.0	41.0	34.0	41.0
17	38.37324281966543	40.0	38.0	41.0	34.0	41.0
18	38.253294606657356	40.0	38.0	41.0	34.0	41.0
19	38.05993066675752	40.0	37.0	41.0	34.0	41.0
20	38.02135532009131	40.0	36.0	41.0	34.0	41.0
21	37.884917890361486	40.0	36.0	41.0	33.0	41.0
22	37.958500562161426	40.0	36.0	41.0	34.0	41.0
23	37.95740434738169	40.0	36.0	41.0	34.0	41.0
24	37.902867023270076	40.0	35.0	41.0	34.0	41.0
25	37.69392184252666	39.0	35.0	41.0	33.0	41.0
26	37.749401212905866	40.0	35.0	41.0	33.0	41.0
27	37.748084392354606	40.0	35.0	41.0	34.0	41.0
28	37.61250894347722	40.0	35.0	41.0	33.0	41.0
29	37.55148717249838	40.0	35.0	41.0	33.0	41.0
30	37.351272529045005	39.0	35.0	41.0	33.0	41.0
31	37.2398044359647	39.0	35.0	41.0	33.0	41.0
32	37.061967224285375	39.0	35.0	41.0	32.0	41.0
33	36.82114663895608	39.0	35.0	41.0	31.0	41.0
34	36.541607611325	39.0	35.0	41.0	30.0	41.0
35	36.38259684508194	39.0	35.0	41.0	30.0	41.0
36	36.217007938400734	39.0	35.0	41.0	30.0	41.0
37	36.14640983271439	39.0	35.0	41.0	29.0	41.0
38	36.021132159040576	39.0	35.0	41.0	29.0	41.0
39	35.94184014173282	39.0	35.0	41.0	28.0	41.0
40	35.86413069401383	39.0	35.0	41.0	28.0	41.0
41	35.72118241286498	39.0	35.0	41.0	27.0	41.0
42	35.68980869476338	39.0	35.0	41.0	27.0	41.0
43	35.62195581070492	39.0	35.0	41.0	27.0	41.0
44	35.46667660386358	39.0	35.0	41.0	26.0	41.0
45	35.40787792579469	38.0	35.0	40.0	26.0	41.0
46	35.320345984804604	38.0	35.0	40.0	25.0	41.0
47	35.27880566249872	38.0	35.0	40.0	25.0	41.0
48	35.176604715341895	38.0	35.0	40.0	25.0	41.0
49	35.05933869374127	38.0	35.0	40.0	24.0	41.0
50	34.96098599706995	38.0	34.0	40.0	24.0	41.0
51	33.82799052843174	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	26.0
10	25.0
11	22.0
12	19.0
13	30.0
14	42.0
15	57.0
16	91.0
17	198.0
18	365.0
19	664.0
20	1181.0
21	1895.0
22	2970.0
23	4498.0
24	7133.0
25	11891.0
26	17132.0
27	20164.0
28	20037.0
29	19291.0
30	20595.0
31	24202.0
32	30141.0
33	41953.0
34	69127.0
35	83081.0
36	92515.0
37	137422.0
38	229469.0
39	337607.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.89685189601717	18.43403972607407	21.874126946270998	19.794981431637765
2	36.93647575891792	22.479387414398147	23.09342100780212	17.49071581888181
3	30.339170726721402	22.088174167830736	31.294504446185822	16.278150659262035
4	26.020237811318182	24.410667438928826	31.081479336308814	18.487615413444175
5	22.23416578651494	31.942097373172974	28.117185104425747	17.706551735886343
6	23.609928111478315	31.646962624782805	29.519437157166706	15.223672106572177
7	75.53226465878505	5.059793533440087	14.438434806309836	4.969507001465026
8	76.90879356751049	4.189124731695683	13.850209532894961	5.051872167898879
9	70.92969575142244	6.180879697454942	16.39594903069742	6.493475520425199
10	35.44896255664202	26.56791932131784	22.23212156314947	15.750996558890668
11	26.608718612653742	22.76140506285987	31.08301250383292	19.54686382065347
12	23.47747947259037	20.76717999386733	34.807502299751285	20.947838233791014
13	22.690453476883242	21.32559367653572	36.630694013832574	19.35325883274846
14	19.26808285918708	24.370464379407856	35.76726516984089	20.59418759156417
15	18.549112466355492	23.241456849851794	37.77281864331709	20.436612040475623
16	22.477939422847605	23.24733399202753	34.364331709311436	19.91039487581343
17	22.313805321794828	24.037681850703553	32.541310347177266	21.10720248032435
18	24.2323089502913	22.631426527205207	32.727334673435315	20.408929849068176
19	22.084256073046916	26.190760110388062	30.495468638206535	21.229515178358486
20	23.14597458348949	25.397686620558073	30.816837586453616	20.639501209498825
21	22.461841163844504	26.197233484378728	31.97429389117918	19.366631460597596
22	22.159040577833807	22.537477428367005	32.36235562672481	22.94112636707438
23	20.422728356785118	25.837279820108343	32.085107832782526	21.65488399032401
24	20.57238254233246	23.88351333855746	33.205768116929576	22.338336002180505
25	22.055807297877415	25.481670130489594	30.60662328370413	21.855899287928864
26	20.753551838097508	24.534768832407757	31.01955640352969	23.692122925965045
27	21.445095567442333	23.787605192327348	31.446202855098633	23.321096385131682
28	20.12359033763756	25.34061871827195	31.31741678307383	23.218374161016662
29	20.95397090388743	25.666842697012026	30.449388436509828	22.929797962590712
30	22.147286293482335	24.50734216892099	30.889833395795712	22.45553814180096
31	22.273857790194544	25.605515996047835	28.778150659262035	23.342475554495586
32	21.422268406527888	26.369800006814074	29.76261456168444	22.445317024973598
33	21.068873292221728	24.492351197574187	29.587322408095122	24.851453102108955
34	19.693536847126165	25.774675479540733	30.533542298388472	23.998245374944634
35	20.37034513304487	25.824929303941946	29.46023985554155	24.344485707471637
36	21.781285135089092	27.3416578651494	27.644373275186535	23.232683724574972
37	21.00149909713468	25.89255902694968	29.279326087697182	23.826615788218458
38	21.339988416067595	25.812152907907738	29.625907124118427	23.22195155190624
39	21.53487104357603	23.500136281557698	29.553337194644136	25.41165548022214
40	23.64246533337876	22.664304452999897	30.39947531600286	23.29375489761848
41	20.81590065074444	24.212548124425062	30.05740860618037	24.914142618650132
42	21.70564887056659	25.093182515076144	30.403137882865998	22.798030731491263
43	21.152430922285443	24.227624271745427	31.225171203706857	23.394773602262273
44	22.010834383837008	24.03359340397261	29.92410820755681	24.031464004633573
45	21.73580116520732	23.11829239208204	29.540134918742122	25.605771523968517
46	21.987922046948995	23.950376477803143	28.828148955742567	25.2335525195053
47	21.010527750332187	24.516285646144937	30.48703621682396	23.98615038669892
48	20.679108037204866	24.522247964294234	30.530731491260944	24.26791250723996
49	22.111682736533677	23.164883649620116	30.748952335525193	23.97448127832101
50	20.175292153589318	23.28694081973357	32.043456781711015	24.4943102449661
51	20.256805560287557	22.246942182549144	30.326223978740074	27.170028278423224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	528.0
1	1213.5
2	1899.0
3	14699.5
4	27500.0
5	17982.0
6	8464.0
7	8225.5
8	7987.0
9	7928.5
10	7870.0
11	7718.5
12	7567.0
13	7296.5
14	7026.0
15	6603.5
16	6181.0
17	5941.5
18	5702.0
19	5513.0
20	5324.0
21	5261.5
22	5199.0
23	5383.0
24	5567.0
25	6049.5
26	7781.0
27	9030.0
28	10241.0
29	11452.0
30	12637.0
31	13822.0
32	15979.5
33	18137.0
34	21555.0
35	24973.0
36	27358.0
37	29743.0
38	33215.0
39	36687.0
40	38885.0
41	41083.0
42	46129.0
43	51175.0
44	58835.5
45	66496.0
46	82681.0
47	98866.0
48	107535.5
49	116205.0
50	115758.5
51	115312.0
52	103018.0
53	90724.0
54	81872.5
55	73021.0
56	67180.0
57	61339.0
58	57240.5
59	53142.0
60	48211.5
61	43281.0
62	39093.5
63	34906.0
64	30561.5
65	26217.0
66	22469.0
67	18721.0
68	15630.5
69	12540.0
70	10729.0
71	8918.0
72	7573.5
73	6229.0
74	5159.0
75	3347.0
76	2605.0
77	1857.5
78	1110.0
79	771.0
80	432.0
81	324.0
82	216.0
83	169.0
84	122.0
85	88.0
86	54.0
87	42.5
88	31.0
89	20.0
90	9.0
91	7.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1174040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.349086967288336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36810294799747	26.66863554681724
2	9.408051321052767	6.83948151323318
3	3.813850508329058	4.158899514224693
4	2.1641623800052554	3.1466130624857898
5	1.495536442964627	2.718069211403514
6	1.183950110709188	2.582130332345936
7	0.9678642102714413	2.462668624617773
8	0.8327878828021859	2.4216863341824464
9	0.7236545947932344	2.3673765420375372
>10	5.926422424246553	38.76389395716166
>50	0.07916015809877003	1.9124693558365304
>100	0.0318998914613078	2.191079413976068
>500	0.0016789416251017687	0.40252321939862595
>1k	0.0026383368394456365	1.9253460639374609
>5k	0.0	0.0
>10k+	2.3984880358596697E-4	1.4391273083415896
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16507	1.4059997955776635	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTT	3496	0.2977752035705768	No Hit
CGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTG	3065	0.26106435896562297	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	2685	0.22869748901229942	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCT	2379	0.2026336411025178	No Hit
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	1903	0.16208987768730196	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	1765	0.15033559333583182	No Hit
CGTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCT	1743	0.14846172191748153	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	1560	0.13287451875574938	No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT	1407	0.11984259480085858	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2587986780688906E-4	0.0	0.0	0.12682702463289156	0.0
2	4.2587986780688906E-4	0.0	0.0	0.49879050117542845	0.0
3	4.2587986780688906E-4	0.0	0.0	0.9384688766992607	0.0
4	4.2587986780688906E-4	0.0	0.0	1.4082995468638206	0.0
5	4.2587986780688906E-4	0.0	0.0	2.552809103608054	0.0
6	4.2587986780688906E-4	0.0	0.0	4.176092807740792	0.0
7	4.2587986780688906E-4	0.0	0.0	5.418725086027734	0.0
8	4.2587986780688906E-4	0.0	0.0	7.171476269973766	0.0
9	4.2587986780688906E-4	0.0	0.0	8.215818881809819	0.0
10	4.2587986780688906E-4	0.0	0.0	9.628036523457464	0.0
11	4.2587986780688906E-4	0.0	0.0	10.87841981533849	0.0
12	4.2587986780688906E-4	0.0	0.0	11.979830329460667	0.0
13	5.110558413682669E-4	0.0	0.0	12.580576470989064	0.0
14	5.110558413682669E-4	0.0	0.0	12.95381758713502	0.0
15	5.110558413682669E-4	0.0	0.0	13.289496098940411	0.0
16	5.110558413682669E-4	0.0	0.0	13.774573268372457	0.0
17	5.110558413682669E-4	0.0	0.0	14.33102790364894	0.0
18	5.110558413682669E-4	0.0	0.0	14.982453749446357	0.0
19	5.110558413682669E-4	0.0	0.0	15.396664508875336	0.0
20	5.110558413682669E-4	0.0	0.0	15.816582058532928	0.0
21	5.110558413682669E-4	0.0	0.0	16.285475792988315	0.0
22	5.110558413682669E-4	0.0	0.0	16.805560287554087	0.0
23	5.110558413682669E-4	0.0	0.0	17.324281966542877	0.0
24	5.110558413682669E-4	0.0	0.0	17.762341998569045	0.0
25	5.110558413682669E-4	0.0	0.0	18.153214541242207	0.0
26	5.110558413682669E-4	0.0	0.0	18.541276276787844	0.0
27	5.110558413682669E-4	0.0	0.0	18.957871963476542	0.0
28	5.110558413682669E-4	0.0	0.0	19.374552826138803	0.0
29	5.110558413682669E-4	0.0	0.0	19.793192736192974	0.0
30	5.962318149296446E-4	0.0	0.0	20.341385302034002	0.0
31	6.814077884910225E-4	0.0	0.0	20.78625941194508	0.0
32	6.814077884910225E-4	0.0	0.0	21.280620762495314	0.0
33	7.665837620524003E-4	0.0	0.0	21.754965759258628	0.0
34	8.517597356137781E-4	0.0	0.0	22.23288814691152	0.0
35	8.517597356137781E-4	0.0	0.0	22.732104527954753	0.0
36	8.517597356137781E-4	0.0	0.0	23.166161289223535	0.0
37	8.517597356137781E-4	0.0	0.0	23.628496473714694	0.0
38	8.517597356137781E-4	0.0	0.0	24.15241388709073	0.0
39	8.517597356137781E-4	0.0	0.0	24.849408878743485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACT	20	7.0333487E-4	45.0	26
CTACGCG	20	7.0333487E-4	45.0	1
CGTTTTT	8275	0.0	42.770393	1
TGCGAAG	70	0.0	41.785713	1
CGTTATT	655	0.0	39.50382	1
GCTAACG	40	3.458299E-7	39.375	1
TACGCGG	110	0.0	38.863636	2
TCGATAG	35	6.248403E-6	38.571426	1
CTAACGG	65	9.094947E-12	38.076927	2
TACGTAG	60	1.5643309E-10	37.500004	1
ACGGGAC	270	0.0	37.5	5
TGCGTAG	55	2.750312E-9	36.81818	1
CACAACG	445	0.0	36.40449	12
TACGGGA	415	0.0	36.325302	4
GCGTTAG	100	0.0	36.000004	1
ACGATAG	25	0.0021071266	36.000004	1
TGCGATC	25	0.0021071266	36.000004	19
TACGAAT	100	0.0	36.000004	12
CGAATTC	25	0.0021071266	36.000004	13
CATACCG	25	0.0021071266	36.000004	2
>>END_MODULE