FastQCFastQC Report
Sat 14 Jan 2017
SRR2935889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375595
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC30190.8037913177757957RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC27790.7398927035769912No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG25160.6698704721841345No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC23200.6176866039217774RNA PCR Primer, Index 10 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC21060.56071033959451RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT13580.3615596586749025TruSeq Adapter, Index 10 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.25559445679521825No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC8330.2217814401150175RNA PCR Primer, Index 35 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT5870.15628536056124284No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC5830.15522038365792942TruSeq Adapter, Index 10 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4920.13099215910754936No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT4900.13045967065589265No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG4500.11980990162275855TruSeq Adapter, Index 10 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC4040.10756266723465435No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT3970.10569895765385588TruSeq Adapter, Index 10 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTA3830.10197153849225896No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATAC207.0272136E-445.00000430
GCGAATT207.0272136E-445.00000415
GTTTACG302.1615833E-645.0000041
ATGGACG207.0272136E-445.0000041
CGTGACG302.1615833E-645.00000419
ATGTGCG207.0272136E-445.0000041
GAATCGG207.0272136E-445.0000041
TGAACCG207.0272136E-445.00000436
CAATTTA207.0272136E-445.00000433
TAAGTAA207.0272136E-445.00000419
TAACGCC406.7939254E-945.00000412
AGGCGAA207.0272136E-445.00000413
CATTCGT207.0272136E-445.0000047
CTACGCG207.0272136E-445.0000041
TTAACGG406.7939254E-945.0000042
CTCGCAA207.0272136E-445.00000420
ACCACGC207.0272136E-445.00000430
CCGCGTT207.0272136E-445.00000439
CCGCGCG207.0272136E-445.00000419
CCTATTA207.0272136E-445.00000412
Produced by FastQC (version 0.11.5)