FastQCFastQC Report
Sat 14 Jan 2017
SRR2935887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068190
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251712.356415993409412No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC116111.0869789082466603No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT68980.6457652664788099No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC53480.5006599949447196No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT43990.4118181222441701No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT42410.3970267461781144No Hit
CGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG40690.38092474185304115No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG36710.34366545277525534No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT35610.3333676593115457No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC34700.32484857562793135No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT26580.2488321366049111No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC25250.236381168144244TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC17230.16130089216337917No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC15920.14903715631114314TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC14080.1318117563354834No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.12956496503431036No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGT13380.12525861504039545No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT12370.11580336831462568TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC11590.1085012965858134No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT11050.10344601615817411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT137350.044.0826341
CGTTATT8950.042.234641
CGTTCTG6650.041.616541
CGTTTCT8450.041.538461
CTAACGG950.040.2631572
CGTTTTC5250.039.4285741
TTAGACG403.457917E-739.3751
ACGTCGC403.457917E-739.37520
TAGTGCG403.457917E-739.3751
GATTACG403.457917E-739.3751
GTTTTTT153350.039.1017272
GACGTCG356.247874E-638.5714319
AAGGGCG3200.037.968755
GCGAAGG2750.037.6363642
TGTTCGG301.13986775E-437.5000042
TACTCGC301.13986775E-437.50000445
TACGTAG552.748493E-936.818181
GCGATCG800.036.56259
ACGGGCC1300.036.3461575
TAACGCG250.00210704236.01
Produced by FastQC (version 0.11.5)